African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Decontamination of ready-to-eat Japanese mustard green (Brassica japonica) from Escherichia coli using slightly acidic electrolyzed water

Abdulsudi Issa-Zacharia1,2,3*, Yoshinori Kamitani1, Kazuo Morita1 and Koichi Iwasaki1
1Department of Environmental Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. 2United Graduate School of Agriculture-Kagoshima University, Laboratory of Food Biosystems and Environment, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. 3Department of Food Science and Technology, Sokoine University of Agriculture, P. O. Box 3006, Morogoro, Tanzania.
Email: [email protected]

  •  Accepted: 02 September 2009
  •  Published: 31 October 2009

Abstract

In the present study, slightly acidic electrolyzed water (SAEW: pH 5.6 ± 0.21, 22 ± 1.4 mg/L available chlorine concentration; ACC) was evaluated for decontamination of Japanese mustard green (Brassica japonica) from Escherichia coli (E. coli) and its effectiveness was compared to that of sodium hypochlorite solution (NaOCl solution: pH 9.8±0.01, 110 ± 2.3 mg/L ACC). Decontamination of Japanese mustard green inoculated with E. coli was done by dipping samples into SAEW, NaOCl solution and Tap water (control) with stirring for 2, 5, 10 and 15 min. SAEW achieved E. colireduction of 1.29 -1.64 log10 CFU/g and was not significantly different from that achieved by NaOCl solution (P > 0.05). Increasing the exposure time did not significantly affect its decontamination effectiveness. The results of current study indicate that SAEW with near neutral pH and low available chlorine concentration could be used as an alternative food sanitizer for NaOCl solution to reduce the population of pathogens from the fruits and vegetables in food industry.

 

Key words: Slightly acidic electrolyzed water, ready-to-eat vegetables, decontamination, Escherichia coliMizuna.