African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Characterization of Aspergillus section Nigri isolates from leaf litter and soil in the Atlantic Forest of Brazil

Tatiana Faria Maia
  • Tatiana Faria Maia
  • Programa de Pos-graduacco em Fitossanidade e Biotecnologia Aplicada, Universidade Federal Rural do Rio de Janeiro, Brazil
  • Google Scholar
Jean Luiz Simoes-Araujo
  • Jean Luiz Simoes-Araujo
  • Embrapa Agrobiologia, Brazil
  • Google Scholar
Carlos Antonio Inacio
  • Carlos Antonio Inacio
  • Departamento de Entomologia e Fitopatologia, Universidade Federal Rural do Rio de Janeiro, Brazil
  • Google Scholar
Marcelo Elias Fraga*
  • Marcelo Elias Fraga*
  • Departamento de Microbiologia e Imunologia Veterinaria, Universidade Federal Rural do Rio de Janeiro, Brazil
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  •  Received: 17 September 2014
  •  Accepted: 01 December 2014
  •  Published: 04 February 2015

Abstract

Section Nigri is among the most important from the genus Aspergillus, since it possesses economical and biotechnological properties. Its identification is considered quite hard and complex. Due to this fact, Aspergillus taxonomy has been carried out by morphological, biochemical and molecular data. Morphological characterization besides molecular analyses was performed to properly identify Aspergillus section Nigri. A set of 42 Aspergillus section Nigri isolates were obtained from Brazilian Atlantic Rainforest. From these isolates, 16.7% originated from leaf litter layer and 83.3%, from soil. The isolates were divided into three different groups according to analyzed characteristics. 26 were identified as Aspergillus aculeatus, 12 as Aspergillus aculeatinus and 4 belonged to a group called “Aspergillus niger Aggregate”, considered as biseriate.

Key words: Fungi, taxonomy, morphological identification, polymerase chain reaction (PCR), ITS.