African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5200

Full Length Research Paper

Isolation and application of a chlorpyrifos-degrading Bacillus licheniformis ZHU-1

Jiangwei Zhu1, Yan Zhao2 and Jiangping Qiu1*
  1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China. 2Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, PR China.
Email: [email protected]

  •  Accepted: 07 October 2010
  •  Published: 18 November 2010



A strain ZHU-1 capable of utilizing chlorpyrifos as the sole carbon sources and energy was isolated from soil. ZHU-1 was identified as Bacillus licheniformis based on analysis of morphology, physiological and biochemical characters and 16S rRNA. The addition of ZHU-1 to soil treated with chlorpyrifos resulted in a higher degradation rate than noninoculated soils, the degradation rate of chlorpyrifos (100 mg kg-1) could reach 99% or above after 14 days. The microbial manure added by strain ZHU-1 can be applied not only as fertilizer, but also in degrading chlorpyrifos residue in soil. This study may provide basis for prevention and control of pesticides pollution.


Key words: Chlorpyrifos, degradation, Bacillus licheniformis, microbial manure.