African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Seasonal influence on bioactivity of seaweeds against plant pathogenic bacteria Xanthomonas axonopodis pv. citri (Hasse) Vauterin et al.

Kulanthaiyesu Arunkumar* and Sadaiyappa Ramasubbu Sivakumar      
Marine Algal Research Division, Department of Botany, Alagappa Government Arts College (Alagappa University), Karaikudi-630 003, Sivagangai-Dt, Tamil Ndu, India.  
Email: Tel: -91-4565224521. Fax: -91-4565227497

  •  Accepted: 25 January 2012
  •  Published: 30 May 2012

Abstract

In the present study, thin-layer chromatography (TLC) method was adopted for the fractionation of antibacterial substances from the crude extracts obtained in 1:1(v/v) chloroform : methanol of red seaweeds comprising Portieria hornemannii (Lyngbye) P. Silva, Gracilaria crassa (Harvey) J. Ag., brown Dictyota bartyrensiana Lamouroux,Hydroclathrus clathratus (C.Agardh) M.A.Howe, Sargassum wightii Greville, green Ulva (Enteromorpha) compressa (Linnaeus) Nees, and Halimeda macroloba Decaisne collected along the Coast of Pamban (Rameswaram, Gulf of Mannar, India) at four different seasons namely post-monsoon (February), summer (May), pre-monsoon (August) and monsoon (November) seasons for the year 2009. Fractionated substances detected under UV (Mechanical + disinfection) and iodine were tested for antibacterial activity against the plant pathogenic bacterium Xanthomonas axonopodis pv. citri (Hasse) Vauterin et al. which cause canker in citrus. Number of active fractions with high antibacterial activity was recorded from the crude extracts obtained in seaweeds collected during monsoon and post-monsoon whereas seaweeds collected during summer and pre-monsoon showed low antibacterial activity. UV active substances fractionated at R0.23 from red seaweedPorteiria hornemannii showed maximum antibacterial activity among the fractionated substances obtained on TLC from the crude extracts of all the seaweeds investigated.

 

Key words: Seaweeds, antibacterial activity, Xanthomonas axonopodis pv. Citri, thin-layer chromatography (TLC) fractions.