African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Diagnostic value of two HIV rapid diagnostic tests 16 years after their adoption in national strategies in the Central African Republic (CAR)

Christian Diamant Mossoro-Kpinde
  • Christian Diamant Mossoro-Kpinde
  • Laboratoire National de Biologie Clinique et de Santé Publique, Bangui, Central African Republic.
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Thierry Mbesse
  • Thierry Mbesse
  • Faculté des Sciences de la Santé, Université de Bangui, Bangui, Central African Republic.
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Christelle Bobossi
  • Christelle Bobossi
  • Ecole doctorale des Sciences et Techniques, Faculté des Sciences, Université de Bangui, Bangui, Central African Republic.
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Coretha Bokia-Baguida
  • Coretha Bokia-Baguida
  • Ecole doctorale des Sciences et Techniques, Faculté des Sciences, Université de Bangui, Bangui, Central African Republic.
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Simplice Arthur Sombot
  • Simplice Arthur Sombot
  • Ecole doctorale des Sciences et Techniques, Faculté des Sciences, Université de Bangui, Bangui, Central African Republic.
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Nina Esther Ontsira Ngoyi
  • Nina Esther Ontsira Ngoyi
  • Laboratoire de Bactériologie-Virologie du Centre Hospitalier Universitaire de Brazzaville, Republic of Congo.
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Hermione Dahlia Mossoro-Kpinde
  • Hermione Dahlia Mossoro-Kpinde
  • Faculté des Sciences de la Santé, Université de Bangui, Bangui, Central African Republic.
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Rosine Feissona
  • Rosine Feissona
  • Laboratoire National de Biologie Clinique et de Santé Publique, Bangui, Central African Republic.
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Gérard Gresenguet
  • Gérard Gresenguet
  • Faculté des Sciences de la Santé, Université de Bangui, Bangui, Central African Republic.
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  •  Received: 23 June 2021
  •  Accepted: 15 September 2021
  •  Published: 17 November 2021

Abstract

The broad genetic diversity of HIV requires regular reassessment of adopted HIV diagnostic tests. The objective of this study was to reassess the performances of Determine® HIV 1/2 and Uni-gold® HIV1/2, 2 HIV rapid diagnostic tests of the national algorithm, adopted since 2005 in Central African Republic. A reference prospective panel of 150 plasma samples were tested in duplicate with Determine® HIV 1/2 and Uni-gold® HIV, according to reference serological immune-enzymatic method. Analytical and virological characteristics were calculated. Sensitivity, specificity, positive and negative predictive values (PPV, NPV) of Determine® HIV 1/2 were 100%, as well as the specificity and NPV of Uni-gold® HIV 1/2. Uni-gold® HIV 1/2 sensitivity and PPV were 96 and 92%, respectively. The Cohen k coefficient was close to 1 for the 2 tests, 1 for Determine® HIV 1/2 and 0.97 for Uni-gold® HIV 1/2. Except for the sensitivity of Uni-gold® HIV 1/2, the performances of 2 tests were acceptable and in perfect agreement with the reference tests. These results require a revision of the algorithm. In addition, an exploration in molecular biology is indicated to determine the subtypes of the HIV strains of the 4 samples that tested false negative with Uni-gold® HIV 1/2.

 

Key words: HIV, rapid diagnosis test, sensitivity, specificity, Africa, Central African Republic.