African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Cloning and expression of Bacillus thuringiensis cry1Ia in Escherichia coli and its insecticidal activity

Gunjan Bharti*
  • Gunjan Bharti*
  • Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad - 05, Karnataka. India
  • Google Scholar
P.U. Krishnaraj
  • P.U. Krishnaraj
  • Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad - 05, Karnataka. India
  • Google Scholar


  •  Received: 08 July 2014
  •  Accepted: 22 August 2014
  •  Published: 03 September 2014

References

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Bergamasco VB, Goncalves JF, Polanczk RA, Desiderio JA, Lemos MV (2011) . Expression of a new Bacillus thuringiensis cry1Ia gene in Escherichia coli with strong activity against cotton pests. Aust. J. Basic Appl. Sci. 5(12): 526-533.

 
 

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Cheng JC, Hsieh FC, Liu BL, Kao SS (2005) . Cloning and expression of Cry1Ab, Cry1C and Cry1Da genes from Bacillus thuringiensis var. aizawai., 6th Pacific conference on the Biotechnology of Bacillus thuringiensis and its Environment impact, Victoria BC.

 
 

Choi SK, Shin BS, Kong EM, Rho HM, Park SH (2000). Cloning of a new Bacillus thuringiensis Cry1I-type crystal protein. Curr. Microbiol. 41: 6569.
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Crickmore N, Zeigler DR, Schnepf E, Van Rie J, Lereclus D, Baum J,Bravo A, Dean DH (1998). Bacillus thuringiensis toxin nomenclature[online]http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/index.html

 
 

Gleave AP, Williams R, Hedges RJ (1993). Screening by polymerase chain reaction of Bacillus thuringiensis serotypes for the presence of CryV-like insecticidal protein genes and characterization of a cryV gene cloned from Bacillus thuringiensis subsp kurstaki. Appl. Environ. Microbiol. 59:1683- 1687.
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Grossi-de-Sa MF, Magalhaes MQ, Silva MS, Silva SMB, Dias SC, Nakasu YET, Brunetta PSF, Oliveira GR, Oliveira OB, Neto RS, Oliveira LHB, Soares MAZ, Ayub HA., Siqueira A, Figueira ELZ (2007) . Susceptibility of Anthonomus grandis (Cotton Boll Weevil) and Spodoptera frugiperda (Fall Armyworm) to a Cry1Ia-type Toxin from a Brazilian Bacillus thuringiensis Strain. J. Biochem. Mol. Biol. 40(5):773-782.
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Kuo WS, Chak KF (1996). Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA. Appl. Environ. Microbiol. 62(4):1369-1377.
Pubmed

 
 

Lamptey J, Hendrick CV, Tomes NJ, Brown S, Dean DH (1991).Bacillus. In: Levin MA, Seider RJ, Rogul M (eds) Microbial Ecology :Principles, Methods and Applications, McGraw-Hill, New York. pp.20-22.

 
 

Lorence A, Darszon A, Quintero R, Diaz C, Lievano A, Bravo A (1995) . Delta- endotoxins induce cation channels in Spodoptera frugiperda brush border membranes in suspension and in planar lipid bilayers. FEBS Lett. 360:217-222.
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Pietrantonio PV, Gill SS (1996). Bacillus thuringiensis delta -endotoxins : action on the insect midgut (eds. M.J. Lehane and P.F. Billingsley) . Chapman and Hall, London. pp. 345-372 .

 
 

Pooja AS, Krishnaraj PU, Prashanthi SK (2013). Profile of cry from native Bacillus thuringiensis isolates and expression of Cry1I. Afr. J. Biotechnol.12(22):3545-3562.

Academic Journals

 
 

Santos RC, Marcellino LH, Monnerat RG, Gander EG (2003). Mechanical damage in cotton buds caused by the boll weevil. Pesquisa Agropecuária Brasileira. 38:1351-1355
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Sambrook J, Russell DW (2001) . Molecular Cloning: A laboratory Manual. Cold Spring Harbor Laboratory Press.

 
 

Schwartz JL, Masson L, Brousseau R, Rousseaeu E (1993). Lepidopteran specific crystal toxins from., Garneau Bacillus thuringiensis form cation and anion selective channels in planar lipid bilayers . J. Membr. Biol. 132: 53-62.
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Selvapandiyan A, Reddy VS, Kumar PA, Tewari KK, Bhatnagar RK (1998) . Transformation of Nicotiana tabacum with a native cry1Ia5 gene confers complete protection against Heliothis armigera. Mol. Breed. 4:473-478.
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Song F, Zhang J, Gu A, Wu Y,Han L, He K, Chen Z, Yao J, Hu Y, Li G, Huang D (2003). Identification of cry1I type genes from Bacillus thuringiensis strains and characterization of a novel cry1I type gene. Appl. Environ. Microbiol. 69(9):5207-5211.
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Tounsi S, Zouari N, Jaoua S (2003). Cloning and study of the expression of a novel cry1Ia-type gene from Bacillus thuringiensis subsp kurstaki. J. Appl. Microbiol. 95:23-28.
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