African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5234

Full Length Research Paper

Molecular typing of Salmonella enterica serovars Typhimurium and Enteritidis isolated from Taif area of Saudi Arabia by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)

Ohud M. Al-Harthi
  • Ohud M. Al-Harthi
  • Biotechnology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia
  • Google Scholar
Hayam S. Abdelkader*
  • Hayam S. Abdelkader*
  • 1. Biotechnology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia; 2. Microbiology and Immunology Department, Faculty of Pharmacy, Modern University for Technology and Information, Egypt.
  • Google Scholar
and Eman M. Halawani
  • and Eman M. Halawani
  • Biology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia
  • Google Scholar


  •  Accepted: 09 February 2015
  •  Published: 04 March 2015

References

Abdullahi M (2010). Incidence and antimicrobial susceptibility pattern of Salmonella species in children attending some hospitals in Kano Metropolis, Kano State –Nigeria. Bayero. J. Pure Appl. Sci. 3(1): 202 – 206.

 

Al-Nakhli HM, Al-Ogaily ZH, Nassar TJ (1999). Representative Salmonella serovars isolated from poultry and poultry environments in Saudi Arabia. Rev. Sci. Tech. Int. Epiz. 18 (3): 700-709.

 
 

Ammari S, Laglaoui A, En-nanei L, Bertrand S, Wildemauwe C, Barrijal S, Abid M (2009). Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco. J. Infect. Dev. Ctries. 3(9): 695-703.
Crossref

 
 

Anderson ES, Ward LR, De Saxe MJ, De Sa JDH (1977). Bacteriophage typing designations of Salmonella Typhimurium. J. Hyg. 78(2): 297-300.
Crossref

 
 

Andrews HL, Baumler AJ (2005). Salmonella species. In: Fratamico, P. M., Bhunia, A. K. and Smith, J. L. (Eds.). Foodborne pathogens: Microbiology and Molecular Biology. United Kingdom: Horizon Scientific Press Ltd. pp. 327-339.

 
 

Bhunia AK (2008). Foodborne microbial pathogens: Mechanisms and pathogenesis. United States of America: Springer Science. Business Media, LLC.

 
 

Boyen F, Haesebrouck F, Maes D, Van Immerseel F, Ducatelle R, Pasmans F (2008). Non-typhoidal Salmonella infections in pigs: a closer look at epidemiology, pathogenesis and control. Vet. Microbiol. 130(1-2): 1-19.
Crossref

 
 

Bugarel M, Granier SA, Weill FX, Fach P, Brisabois A (2011). A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium. BMC Microbiology 27(11):151.
Crossref

 
 

CDC, Centers for Disease Control and Prevention (2011). Vital signs: incidence and trends of infection with pathogens transmitted commonly through food - Foodborne diseases active surveillance network, 10 U.S. sites, 1996- 2010. MMWR 60(22):749-755.
Pubmed

 
 

Chansiripornchai N, Ramasoota P, Bangtrakuknonth A. Sasipreeyajan J, Svenson SB (2000). Application of romly amplified polymorphic DNA (RAPD) analysis for typing avian Salmonella enterica subsp enterica. FEMS. Immun. Med. Microbiol. 29(3):221-225.
Crossref

 
 

Crump JA, Medalla FM, Joyce KW, Krueger AL, Hoekstra RM, Whichard JM, Barzilay EB, Emerging Infections Program NARMS Working Group (2011). Antimicrobial resistance among invasive nontyphoidal Salmonella Enterica isolates in the United States: National Antimicrobial Resistance Monitoring System 1996 to 2007 Antimicrob. Agents Chemother. 55(3): 1148–1154.
Crossref

 
 

Dos Santos LR, Ribeiro RA, de Oliveira SD, Rodrigues LB, Flores ML, Lopes RFF, do Nascimento VP (2008). RAPD/PCR phage typing of Salmonella enteritidis isolated from poultry food poisoning outbreaks. Sao Paulo Arq. Inst. Biol. 75: 91-94.

 
 

Franklin K, Lingohr EJ, Yoshida C, Anjum M, Bodrossy L, Clark CG, Kropinski AM, Karmali MA (2011). Rapid genoserotyping tool for classification of Salmonella serovars. J. Clin. Microbiol. 49(8):2954-65.
Crossref

 
 

Garaizar J, Lopez-Molina N, Laconcha I, Lau Baggesen D, Rementeria AVivanco A, Audicana A, Perales I (2000). Suitability of PCR fingerprinting infrequentrestriction- site PCR pulsed-field gel electrophoresis combined with computerized gel analysis in library typing of Salmonella enterica serovar Enteritidis. Appl. Environ. Microbiol. 66(12):5273-5281.
Crossref

 
 

Grassl GA, Finlay BB (2008). Pathogenesis of enteric Salmonella infections. Curr. Opin. Gastroenterol. 24(1):22-26.
Crossref

 
 

Grimont PAD, Weill FX (2007). Antigenic Formulas of the Salmonella Serovars 9th edition WHO Collaborating Centre for Reference Research on Salmonella Paris: Pasteur Institute Paris France 1-67.

 
 

Halawani E, Shohayeb M (2006). Epidemiological Typing of Salmonellla enterica Isolates Causing Acute Food Poisoning in Saudi Arabia Based on Plasmid Profiles Antibiograms. Am. Eur. J. Sci. Res. 3(2): 178-187.

 
 

Halawani E, Shohayeb M (2008). Molecular Characterization of Multiple Antibiotic Resistance in Salmonella enterica Serovar Typhimurium Eenteritidis Isolated in Saudi Arabia. World. J. Med. Sci. 3(2):65-70.

 
 

Hanes D (2003). Nontyphoid Salmonella in Henegariu O Heerema NA, Dlough, Vance GH, Vogt PH (Eds). Int. book of foodborne pathogens. New York, pp.146-158.

 
 

Hilton AC, Banks JG, Penn CW (1996). Rom amplification of polymorphic DNA (RAPD) of Salmonella: strain differentiation characterization of amplified sequences. J. Appl. Bacteriol. 81(6): 575–584.
Crossref

 
 

Hunter PR, Gaston MA (1988). Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26(11):2465-2466.
Pubmed

 
 

Jegadeeshkumar D, Saritha V, Moorthy K, Suresh Kumar BT (2010). Prevalence antibiotic resistance RAPD analysis of food isolates of Salmonella species. Int. J. Biol. Tech. 1:50-55.

 
 

Jin JD, Lee DS, Shin EK, Kim SJ, Jung R, Hanh TW (2006). Molecular typing by rom amplification of polymorphic DNA (RAPD) detection of virulence genes of S enterica subsp enterica serovar Gallinarum biovar Gallinarum. J. Vet. Med. Sci. 68(2):1321-1326.
Crossref

 
 

Lee HY, Su LH, Tsai MH, Kim SW, Chang HH, Jung SI, Park KH, Perera J, Carlos C, Tan BH, Kumarasinghe G, So T, Chongthaleong A, Hsueh PR, Liu JW, Song JH, Chiu CH (2009). High rate of reduced susceptibility to ciprofloxacin ceftriaxone among nontyphoid Salmonella clinical isolates in Asia. Antimicrob. Agents Chemother. 53(6): 2696-2699.
Crossref

 
 

Lim H, Lee H, Hong CH, Bahk GJ, Choi WS (2005). Comparison of four molecular typing methods for the differentiation of Salmonella spp. Int. J. Food. Microbiol. 105(3): 411 – 418.
Crossref

 
 

Malik GM, Al-Wabel AA, Ahmed MEK, Bilal NE, Sheno AK, Abdalla M, Mekki TE (1994). Pattern of antibiotic sensitivity of Salmonella isolates in Asir Region, Southern part of Saudi Arabia. Saudi Med. J. 15:48-51.

 
 

Mare L, Dick LM, Verwalt ML (2001). Chansiripornchai N, Ramasoota P, Bangtrakuknonth A. Sasipreeyajan J, Svenson SB (2000). Application of romly amplified polymorphic DNA (RAPD) analysis for typing avian Salmonella enterica subsp enterica FEMS Immun. Med. Microbiol. 29(3):221-225.

 
 

Maripi A, Suresh Raja SS, Ponmurugan P, Gurusubramanian G (2007). Rom Amplification of Polymorphic DNA (RAPD) of Salmonella enteritidis isolated from chicken samples. Biotechnology 6(2):278-282.
Crossref

 
 

Meenu K (2002). Study of heterogeneity in Indian isolates of Salmonella paratyphi B MSc Thesis Indian Veterinary Research Institute UP India.

 
 

Millemann Y, Lesage-Descauses MC, Lafont JP, Chaslus-Dancla E (1996). Comparison of rom amplified polymorphic DNA analysis enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella. FEMS Immunol. Med. Microbiol. 14(2-3):129-134.
Crossref

 
 

Mishu B, Kohler J, Lee LA (1994). Outbreak of Salmonella enteritidis infections in the United States 1985-1991. J. Infect. Dis. 169:547-552.
Crossref

 
 

Morshed R, Peighambari SM (2010). Drug resistance plasmid profile from amplified polymorphic DNA analysis of Iranian isolates of Salmonella Enteritidis. New Microbiol. 33(1):47-56.
Pubmed

 
 

Moussa IM, Ashgan MH, Mahmoud MH, Al-Doss AA (2011). Rapid detection of Salmonella enterica in food of animal origins collected from Riyadh King Saudi Arabia. Afr. J. Microbiol. Res. 5(15): 2173-2178.
Crossref

 
 

Moussa IM, Gassem MA, Al-Doss AA, Mahmoud WA, Abdel Mawgood, AL (2010). Using molecular techniques for rapid detection of Salmonella Serovars in frozen chicken chicken products collected from Riyadh Saudi Arabia. Afr. J. Biotechnol. 9(5):612-619.

 
 

Nabbut NH, Barbour EK, Al-Nakhli HM (1982). Salmonella species serotypes isolated from farm animals animal feed sewage sludge in Saudi Arabia. Bull. World Health Org. 60 (5):803-807.
Pubmed

 
 

Nayak R, Stewart T, Wang RF, Lin J, Cerniglia CE, Kenney PB (2004). Genetic diversity virulence gene determinants of antibiotic-resistant Salmonella isolated from pre-harvest turkey production sources. Int. J. Food Microbiol. 91(1): 51-62.
Crossref

 
 

Ohud MA, Halawani EM, Abdelkader HS (2010). Detection of Salmonella strains in clinical samples from Saudi Arabia by invA and hilA polymerase chain reaction (PCR)-based assays. Afr. J. Microbiol. Res. 6(25):5410-5416.

 
 

Ohud MA, (2012). Molecular Characterization of Salmonella enterica Causing Gastroenteritis by DNA Fingerprinting. MSc.Thesis, Microbiology Department, Faculty of Science, Taif University, p. 252.

 
 

Panhotra BR, Saxena AK, Al-Ghamdi AM (2004). Emerging nalidixic acid ciprofloxacin resistance in non-typhoidal Salmonella isolated from patients having acute diarrhoeal disease. Ann. Saudi Med. 24(5): 332-336.
Pubmed

 
 

Parry CM (2006). Epidemiological clinical aspects of human typhoid fever In Matroeni P, Maskell D (Eds) Salmonella infections: Clinical immunological molecular aspects, New York: Cambridge University Press. pp.1-18.
Crossref

 
 

Pires SM, Vigre H, Makela P, Hald T (2010). Using outbreak data for source attribution of human salmonellosis campylobacteriosis in Europe. Foodborne Pathog. Dis. 7(11):1351-1361.
Crossref

 
 

Pui CF, Wong WC, Chai LC, Tunung R, Jeyaletchumi P, Noor Hidayah MS, Ubong A, Farinazleen MG, Cheah YK, Son R (2011). Salmonella A foodborne pathogen. Int. Food. Res. J. 18:465-473.

 
 

Quintaes BR, Leal NC, Reis EMF, Fonseca EL, Hofero E (2002). Conventional molecular typing of Salmonella Typhi strains from Brazil. Rev. Inst. Med. Trop. S. Paulo. 44(6): 315-319.
Crossref

 
 

Rezk NA, Mansour H, Ghoneim NH, Rifaat MM (2012). Typing of Salmonella Typhi strains isolated from Egypt by RAPD PCR. 3 Biotech. 2:17-25.

 
 

Rodrigues DC, Taxue RV, Rowe B (1990). International increase in Salmonella enteritidis: a new pemic. Epidemiol. Infect. 105(1):21–27.
Crossref

 
 

Stella IS, Muinah AF, Helen AG, Francisca ON, Olusimbo AA, Bolanle O (2011). Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria. Int. J. Mol. Epidemiol. Genet. 2(1):73-77.

 
 

Terajima J, Nakamur A, Watanabe H (1998). Epidemiological analysis of Salmonella enterica Enteritidis isolates in Japan by phage-typing pulsed-field gel electrophoresis. Epidemiol. Infect. 120(3):223-229.
Crossref

 
 

Tsen HY, Lin JS (2001). Analysis of Salmonella enteritidis strains isolated from food-poisoning cases in Taiwan by pulsed field gel electrophoresis plasmid profile phage typing. J. Appl. Microbiol. 91(1):72-79.
Crossref

 
 

Un-Ho J, Tae-Wook C, June-Ki K, Kyung-Soo N, Sang-Do H, Cheorl-Ho K (2000) Differentiation of Salmonella typhimurium from Gram negative Intestinal Microbes by Randomly Amplified Polymorphic DNA (RAPD) Fingerprinting. J. Microbiol. 38(1):8-10.

 
 

Ward LR, De Sa JDH, Rowe B (1987). A phage-typing scheme for Salmonella Enteritidis. Epidemiol Infect. 99(2):291-294.
Crossref

 
 

Weigel RM, Qiao B, Teferedegne B, Suh DK, Barber DA, Isaacson RE, White BA (2004). Comparison of pulsed field gel electrophoresis repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity inferring transmission of Salmonella. Vet. Microbiol. 100(3-4):205-217.
Crossref

 
 

WHO, World Health Organization. (2005). Drug-resistant Salmonella. Fact sheet.no.139. à Salmonella (non-typhoidal) Fact sheet N°139, Updated August 2013.

 
 

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22):6531-6535.
Crossref

 
 

Yang B, Qu D, Zhang X, Shen J, Cui S, Shi Y, Xi M, Sheng, Zhi S, Meng J (2010). Prevalence characterization of Salmonella serovars in retail meats of marketplace in Shaanxi China. Int. J. Food. Microbiol. 141(1-2):63-72.
Crossref

 
 

Yaqoob E, Hussain I, Rahman SU (2007). Molecular characterization by using Rom Amplified Polymorphic DNA (RAPD) analysis of Salmonella enteritidis isolates recovered from avian human sources. Pak. Vet. J. 27:102-104.