African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Molecular typing and cytotoxicity testing of Staphylococcus aureus isolated from milk, meat and clinical sources

Williams L. L.*
  • Williams L. L.*
  • Center of Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, the North Carolina Research Campus, Kannapolis, NC 28081, North Carolina, United States
  • Google Scholar
Ajayi O. A.
  • Ajayi O. A.
  • 2. Department of Food and Animal Sciences, Alabama Agricultural and Mechanical University, Food Microbiology and Immunochemistry Laboratory, Normal, AL. 35762, United States; 3. Department of Food Science and Technology, Bowen University, Iwo, Osun State, Nigeria
  • Google Scholar
Johnson, J.U
  • Johnson, J.U
  • Department of Food and Animal Sciences, Alabama Agricultural and Mechanical University, Food Microbiology and Immunochemistry Laboratory, Normal, AL. 35762, United States
  • Google Scholar
Carter B.
  • Carter B.
  • Center of Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, the North Carolina Research Campus, Kannapolis, NC 28081, North Carolina, United States
  • Google Scholar
Khatiwada J
  • Khatiwada J
  • Center of Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, the North Carolina Research Campus, Kannapolis, NC 28081, North Carolina, United States
  • Google Scholar


  •  Received: 09 December 2013
  •  Accepted: 21 February 2014
  •  Published: 19 March 2014

References

Ajayi O, Williams L, Oluwoye J, Johnson JU (2009). Antimicrobial resistance testing of "Staphylococcus" isolates with spiral gradient endpoint technology. Int. J. Sci. Soc.1:60-69.
 
Ajayi O, Williams LL, Oluwoye J, Johnson JU, Okafor F, Goode Sanders O, Wilson T (2011). Epidemiological Approaches to Food Safety. J. Food Prot. Trends. 31:560-568.
 
Almeida RA, Matthews KR, Cifrian E, Guidry AJ, Oliver SP (1996). Staphylococcus aureus invasion of bovine mammary epithelial cells. J. Dairy Sci. 79:1021-1026.
Crossref
 
Bayles KW, Wesson CA, Liou LE, Fox LK, Bohach GA, Trumble WR (1998). Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells. Infect. Immun. 66:336-342.
Pubmed
 
Bhunia AK (2008). Staphylococcus aureus. Foodborne Microbial Pathogens (Heldman, D. R. editor) Springer Science media, New York, NY pp. 125-134.
 
Buxser S, Bonventre PF (1981). Staphylococcal Enterotoxins Fail to Disrupt Membrane Integrity or Synthetic Functions of Henle 407 Intestinal Cells. Infect. Immunity 929-934.
Pubmed
 
Chye FY, Abdullah A, Ayob MK (2004). Bacteriological quality and safety of raw milk in Malaysia. Food Microbiol. 21:535-541.
Crossref
 
Comprehensive Reviews in Food Science and Food Safety (2006). Antimicrobial Resistance: Implications for the Food System.
 
Dice LR (1945). Measures of the amount of ecologic association between species. Ecology 26:297-302.
Crossref
 
Fan Y, Pan F, Paoli GC, Xiao Y, Sheng H, Shi X (2008). Development of a multiplex PCR method for detection of the genes encoding 16S rRNA, coagulase, methicillin resistance and enterotoxins in Staphylococcus aureus. J. Rapid Methods Autom. Microbiol. 16:394-411.
Crossref
 
FDA-Bacteriological Analytical Manual (FDA, 1998).
 
Foschino R, Invernizzi A, Barucco R, Stradiotto K (2002). Microbial composition, Including the incidence of pathogens, of goat milk from the Bergamo region of Italy during a lactation year. J. Dairy Res. 69:213-225.
Crossref
 
Ghebremedhin B, Layer F, Ko¨nig W, Ko¨nig B (2008). Genetic Classification and distinguishing of Staphylococcus Species Based on Different Partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf Gene Sequences. J. Clini. Microbiol. 46:1019-1025.
Crossref
 
Jablonski LM, Bohach G (2001). Staphylococcus aureus. In: M.P. Doyle, L.R. Beuchat and T.J. Montville, Editors, Food microbiology: fundamentals and frontiers, ASM Press, Washington, DC, pp. 411-434.
 
Jarraud S, Mougel C, ThioulouseJ, Lina G, Meugnier H,Forey F, Nesme X, Etienne J, Vandenesch F (2002). Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect. and Immun. 70:631-641.
Crossref
 
Jonas D, Walev I, Berger T, Liebetrau M, Palmer M, Bhakdi S (1994). Novel path to apoptosis: small transmembrane pores created by staphylococcal a toxin in T lymphocytes evokeinternucleosomal DNA degradation. Infect. Immun. 62:1304-1312.
Pubmed
 
Jørgensen HJ, Mathisen T, Løvseth A, Omoe K, Qvale KS, Loncarevic S (2005). An outbreak of staphylococcal food poisoning caused by enterotoxin H in mashed potato made with raw milk. FEMS Microbiol. 252:267 - 272.
Crossref
 
Karahan M, Cetinkaya B (2006). Coagulase gene polymorphisms detected by PCR in Staphylococcus aureus isolated from subclinical bovine mastitis in Turkey. Vet. J. 174:428-431.
Crossref
 
Lowy FD (2003). Antimicrobial resistance: the example of Staphylococcus aureus. J. Clin. Investigation 111:1265-1273.
Crossref
 
Maldonado Y, Fiser J C, Nakatsu CH, Bhunia AK (2005). Cytotoxicity Potential and Genotypic Characterization of Escherichia coli Isolates from Environmental and Food Sources. Applied and Environ. Microbiol. 71:1890-1898.
Crossref
 
Mason WJ, Blevins JS, Beenken K, Wibowo N, Ojha N, Smeltzer MS (2001). Multiplex PCR Protocol for the Diagnosis of Staphylococcal Infection. J. Clin. Microbiol. 39:3332-3338.
Crossref
 
Menzies BE, Kourteva I (1998). Internalization of Staphylococcus aureus by endothelial cells induces apoptosis. Infect. Immun. 66:5994-5998.
Pubmed
 
Michelim L, Lahude M, Araújo PR, Giovanaz DSH, Müller G, Delamare APL, daCostaSOP Echeverrigaray S (2005). Pathogenicity Factors and Antimicrobial Resistance of Staphylococcus epidermidis associated with nosocomial infections occurring in intensive careunits. Brazilian J. Microbiol. 36:17-23.
Crossref
 
Moran JH, Schnellmann RG (1996). A rapid β-NADH-linked fluorescence assay for lactate dehydrogenase in cellular death. J. Pharm. Toxicological Methods 36:41-44.
Crossref
 
Nı´ Eidhin D, Perkins S, Francois P, Vaudaux P, Ho¨o¨k M, Foster TJ (1998).Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol. Microbiol. 30:245-257.
Crossref
 
Nuzzo I, Sanges MR, Folgore A, Carratelli CR (2000). Apoptosis of human Keratinocytes after bacterial invasion. FEMS Immunol. Med. Microbiol. 27:235-240.
Crossref
 
Olsen JE (2000). DNA-based methods for detection of food-borne bacterial pathogens. Food Res. Int. 33:257-266.
Crossref
 
Peacock SJ, Moore CE, Justice A, Kantzanou M, Story L, Mackie K, O'Neill G, Day NPJ (2002). Virulent Combinations of Adhesin and Toxin Genes in Natural Populations of Staphylococcus aureus. Infect. Immun. 70:4987-4996.
http://dx.doi.org/10.1128/IAI.70.9.4987-4996.2002
PMid:12183545 PMCid:PMC128268
 
Reed KD, Stemper ME, Shukla SK (2007). Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols. Methods in Molecular Biology. Editor Y. Ji. Humana Press Inc. Vol. 391.
 
Revazishvili T, Bakanidze L, Gomelauri T, Zhgenti E, Chanturia G, Kekelidze M, RajannaC, Kreger A, Sulakvelidze A (2006). Genetic Background and Antibiotic Resistance of Staphylococcus aureus Strains isolated in the Republic of Georgia. J. Clin. Microbiol. 44:3477-3483.
Crossref
 
Riyaz-Ul-Hassan S, Vermar V, Nabi Qazi G (2008). Evaluation of three different molecular markers for the detection of Staphylococcus aureus by polymerase chain reaction. Food Microbiol. 25: 452-459.
Crossref
 
Roberts PH, Davis KC, Garstka WR, Bhunia AK (2001). Lactate dehydrogenase release assay from Vero cells to distinguish verotoxin producing Escherichia coli from non-verotoxin producing strains. J. Microbiol. Methods 43:171-181.
Crossref
 
Santos RL, Tsolis RM, Ba¨umler AJ, Smith III R, and Adams LG (2001). Salmonella enterica Serovar Typhimurium Induces Cell Death in Bovine Monocyte-Derived Macrophages by EarlysipB-Dependent and Delayed sipB-Independent Mechanisms. Infection and immunity. 69:2293-2301.
Crossref
 
Siboo IR, Cheung AL, Bayer AS, Sullam PM (2001). Clumping Factor A Mediates Binding of Staphylococcus aureus to Human Platelets. Infect. Immunity 69:3120-3127.
Crossref
 
Suzuki E, Kuwahara-Arai K, Richardson JF, Hiramatsu K (1993). Distribution of mec regulator gene in methicillin-resistant Staphylococcus clinical strains. Antimicrob. Agents Chemother. 37:1219-1226.
Crossref
 
Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B (1995). Interpreting Chromosomal DNA Restriction Patterns Produced by Pulsed-Field Gel Electrophoresis: Criteria for Bacterial Strain Typing. J. Clin. Microbiol. 33:2233-2239.
Pubmed
 
Tristan A, Ying L, Bes M, Etienne J, Vandenesch F, Lina G (2003). Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections. J. Clin. Microbiol. 41:4465-4467.
Crossref
 
Tucker KA, Reilly SS, Leslie CS, Hudson MC (2000). Intracellular Staphylococcus aureusinduces apoptosis in mouse osteoblasts. FEMS Microbiol. Lett. 186:151-156.
Crossref
 
van Belkum A, van Leeuwen W, Kaufmann ME, Cookson B, Forey F, Etienne J, Goering R, Tenover F, Steward C, O'Brien F, Grubb W, Tassios P, Legakis N, Morvan A, El Solh N, De Ryck R, Struelens M, Salmenlinna S, Vuopio-Varkila J, Kooistra M, Talens A, Witte W, Verbrugh H (1998). Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study. J. Clin. Microbiol. 36:1653-1659.
Pubmed
 
Vancraeynest, D. Haesebrouck, F. Hermans, K (2006). Multiplex PCR assay for the detection of high virulence rabbit Staphylococcus aureus strains. Veterinary Microbiol. 121:368-372.
Crossref
 
Waters AE, Contente-Cuomo T, Buchhagen J, Liu CM, Watson L, et al. (2011). Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry. Clin. Infect. Dis 52:1227-1230.
Crossref
 
Wesson CA, Deringer J, Liou LE, Bayles KW, Bohach GA, Trumble WR (2000). Apoptosis induced by Staphylococcus aureus in epithelial cells utilizes a mechanism involving Caspases 8 and 3. Infect. Immun. 68:2998-3001.
Crossref
 
Xie Y, He Y, Gehring A, Hu Y, Li Q, Tu SI, Shi X (2011). Genotypes and Toxin Gene Profiles of Staphylococcus aureus Clinical Isolates from China. PLoS ONE 6(12): e28276. doi:10.1371/journal.pone.0028276.
Crossref
 
Zhang K, Sparling J, Chow BL, Elsayed S, Hussain Z, Church DL, Gregson DB, LouieT, Conly JM (2004). New Quadriplex PCR Assay for Detection of Methicillin and Mupirocin Resistance and Simultaneous Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci. J. Clin. Microbiol. 42:4947-4955.
Crossref