African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Novel simple diagnostic methods compared to advanced ones for the diganosis of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasmas hominis in patients with complicated urinary tract infections

Hala Badawi*
  • Hala Badawi*
  • Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Aisha Abu Aitta
  • Aisha Abu Aitta
  • Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Maisa Omar
  • Maisa Omar
  • Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Ahmed Ismail
  • Ahmed Ismail
  • Urosurgery Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Hanem Mohamed
  • Hanem Mohamed
  • Biochemistry Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Manal El Said
  • Manal El Said
  • Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Doaa Gamal
  • Doaa Gamal
  • Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Samah Saad El Dine
  • Samah Saad El Dine
  • Microbiology Department, Theodor Bilharz Research Institute, Giza, Egypt.
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Mohamed Ali Saber
  • Mohamed Ali Saber
  • Biochemistry Department, Theodor Bilharz Research Institute, Giza, Egypt.
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  •  Accepted: 23 January 2014
  •  Published: 19 February 2014

References

Al-Sweih NA, Al-Fadli AH, Omu AE, Rotimi VO (2012). Prevalence of Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum infections and seminal quality in infertile and fertile men in Kuwait. J. Androl. 33(6):1323-1329.
http://dx.doi.org/10.2164/jandrol.111.013821
PMid:22052774
 
Bakardzhie I, Pehlivanov G, Kovachev E (2011). Correlations between Contemporary Methods in the Diagnosis of Chlamydia Trachomatis urogenital infections. J. IMAB 17(1):158-160. /DOI: 10.5272/jimab.2011171.158
http://dx.doi.org/10.5272/jimab.2011171.158
 
Biernat-Sudolska M, Rojek-Zakrzewska D, Zawilińska B, Magdalena K-V (2013). The need to verify of positive Mycoplasma hominis results obtained using the Mycoplasma IST2 tests. J of Lab Diag, 49 (1):5-8.
 
Centers for Disease Control (CDC) (2010). Sexually transmitted diseases surveillance, 2010.
 
Cheah F-C, Anderson TP, Darlow BA, Murdoch DR (2005). Comparison of the mycoplasma duo test with PCR for detection of Ureaplasma species in endotracheal aspirates from premature infants. J Clin Microbiol, 43(1):509. DOI: 10.1128/JCM.43.1.509-510.2005.
http://dx.doi.org/10.1128/JCM.43.1.509-510.2005
 
Chernesky M, Luinstra K, Sellors J, Schachter J, Moncada J, Caul O, Paul I, Mikaelian L, Toye B, Paavonen J, Mahony J (1998). Can Serology diagnose upper genital tract Chlamydia trachomatis infection? Studies on women with pelvic pain, with or without chlamydial plasmid dna in endometrial biopsy tissue. Sex. Transm. Dis. 25(1):14-19.
http://dx.doi.org/10.1097/00007435-199801000-00004
PMid:9437779
 
Clegg A, Passey M, Yoannes M, Michael A (1997). High rates of genital mycoplasma infection in the highlands of Papua New Guinea determined both by culture and by a commercial detection kit. J. Clin. Microbiol. 35:197-200.
PMid:8968907 PMCid:PMC229538
 
Cunningham KA, Beagley KW (2008). Male genital tract chlamydial infection: Implications for pathology and infertility. Biol. Reprod. 79(2):180-189.
http://dx.doi.org/10.1095/biolreprod.108.067835
PMid:18480466
 
Dean D, Turingan RS, Thomann H-U, Zolotova A, Rothschild J, Joseph SJ, Read TD, Tan E, Selden RF (2012). A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis. PLoS ONE 7(12): e51685. doi:10.1371/ journal.pone.0051685.
 
Ekiel AM, Friedek DA, Romanik MK, Jozwiak J, Martirosian G (2009). Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in women with cervical dysplasia in Katowice, Poland. J. Korean Med. Sci. 24(6):1177-1181.
http://dx.doi.org/10.3346/jkms.2009.24.6.1177
PMid:19949678 PMCid:PMC2775870
 
El-Mishad AM, Mokhtar S, Adu Aitta AM, Ahmed A (1992). Ocurrence of U. urealyticum, M. hominis and anaerobic bacteria in urine of patients with UTI and haematobiasis. Med. J. Cairo Univ. 60(1):167-176.
 
El-Sayed M, Badwy W, Bakr A (2006). Rapid hybridization probe assay and PCR for detection of Chlamydia trachomatis in urinary tract infections: a prospective study, Curr. Microbiol., 53(5):379-383.
http://dx.doi.org/10.1007/s00284-005-0449-4
PMid:17019645
 
Fernández-Benítez C, Mejuto-López P, Otero-Guerra L, Margolles-Martins MJ, Suárez-Leiva P, Vazquez F, Chlamydial Primary Care Group (2013). Prevalence of genital Chlamydia trachomatis infection among young men and women in Spain. BMC Infect. Dis. 13:388.
http://dx.doi.org/10.1186/1471-2334-13-388
PMid:23968487 PMCid:PMC3765329
 
Gaydos CA, Theodore M, Dalesio N, Wood BJ, Quinn TC (2004). Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens. J. Clin. Microbiol. 42(7):3041-3045.
http://dx.doi.org/10.1128/JCM.42.7.3041-3045.2004
PMid:15243057 PMCid:PMC446239
 
Gdoura R, Kchaou W, Chaari C, Znazen A, Keskes L, Rebai T, Hammami A (2008). Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples. J. Androl. 29(2):198-206.
http://dx.doi.org/10.2164/jandrol.107.003566
PMid:18077823
 
Geisler WM (2010). Duration of untreated, uncomplicated Chlamydia trachomatis genital infection and factors associated with chlamydia resolution: a review of human studies. J. Infect. Dis. 201(2):S104-113.
http://dx.doi.org/10.1086/652402
PMid:20470048
 
Ghanaat J, Afshari JT, Ghazvini K, Malvandi M (2008). Prevalence of genital chlamydia in Iranian males with urethritis attending clinics in Mashhad. Eastern Mediterranean Health J. 14(6):1333-1337.
PMid:19161108
 
Ghazvini K, Ahmadnia H, Ghanaat J (2012). Frequency of Chlamydia trachomatis among male patients with urethritis in northeast of iran detected by polymerase chain reaction. Saudi J. Kidney Dis. Transpl. 23(2):316-320.
PMid:22382226
 
González-Pedraza A, Ortiz C, Mota R, Dávila R, Dickinson E (2003). Role of bacteria associated with sexually transmitted infections in the etiology of lower urinary tract infection in primary care. Enferm Infecc. Microbiol. Clin. 21(2):89-92.
http://dx.doi.org/10.1157/13042865
PMid:12586032
 
Goulet M, Dular R, Tully JG, Billowes G, Kasatiya S (1995). Isolation of Mycoplasma pneumoniae from the human urogenital tract. J Clin Microbiol, 33(11):2823-2825.
PMid:8576326 PMCid:PMC228587
 
Hopfe M, Deenen R, Degrandi D, Ko¨hrer K, Henrich B (2013). Host Cell Responses to Persistent Mycoplasmas - Different Stages in Infection of HeLa Cells with Mycoplasma hominis. PLoS ONE 8(1): e54219. doi:10.1371/journal.pone. 0054219.
 
Kamel RM (2013). Screening for Chlamydia trachomatis infection among infertile women in Saudi Arabia. Int J Women's Health, 5: 277-284.
http://dx.doi.org/10.2147/IJWH.S46678
PMid:23785247 PMCid:PMC3682642
 
Kılıç D, Basar MM, kaygusuz S,Yilmaz E, Basar H, Batislam E (2004). Prevelance and treatment of chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in patients with non-gonococcal urethritis. Jpn. J. Infect Dis. (8):17-20.
PMid:14985631
 
Lee Y-S, Lee K-S (2013). Chlamydia and Male Lower Urinary Tract Diseases. Korean J. Urol. 54:73-77.
http://dx.doi.org/10.4111/kju.2013.54.2.73
PMid:23550267 PMCid:PMC3580308
 
Leutscher PDC, Ramarokoto C-E, Hoffmann S, Jensen JS, Ramaniraka V, Randrianasolo B, Raharisolo C, Migliani, R, Christensen N (2008).Coexistence of urogenital schistosomiasis and sexually transmitted infection in women and men living in an area where Schistosoma haematobium is endemic. Clin. Infect. Dis. 47(6):775.
http://dx.doi.org/10.1086/591127
PMid:18680415
 
Maeda S-I, Deguchi T, Ishiko H, Matsumoto T, Naito S, Kumon H, Tsukamoto T, Onodera S, Kamidono S (2004). Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. Int. J. Urol. 11(9):750-754.
http://dx.doi.org/10.1111/j.1442-2042.2004.00887.x
PMid:15379939
 
Mangin D, Murdoch D, Wells JE, Coughlan E, Bagshaw S, Corwin P, Chambers S, Toop L (2012). Chlamydia trachomatis testing sensitivity in midstream compared with first-void urine specimens. Ann. Fam. Med. 10:50-53. doi:10.1370/afm.1323.
http://dx.doi.org/10.1370/afm.1323
 
Marrazo J, Stamm WE (1998). New approaches of chlamydial infection. Curr. Top Clin. Infect. Dis.18:37-59.
 
Nassar F (2007). Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum in Patients with Sterile Pyuria in Gaza-Palestine. Master thesis in Microbiology, Biological Sciences, Faculty of Science, The islamic University-Gaza.
 
Paavonen J, Eggert-Kruse W (1999). Chlamydia trachomatis: impact on human reproduction. Hum. Reprod. Update 5(5):433-447.
http://dx.doi.org/10.1093/humupd/5.5.433
PMid:10582782
 
Sethi S, Singh G, Samanta P, Sharma M (2012). Mycoplasma genitalium: An emerging sexually transmitted pathogen. Indian J. Med. Res. 136(6):942-955.
PMid:23391789 PMCid:PMC3612323
 
Takahashi S, Takeyama K, Miyamoto S, Ichihara K, Maeda T, Kunishima Y, Matsukawa M, Tsukamoto T (2006). Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum DNAs in urine from asymptomatic healthy young Japanese men. J. Infect. Chemother. 12(5): 269-271.
http://dx.doi.org/10.1007/s10156-006-0462-y
PMid:17109090

 

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