African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Production, purification and characterization of polysaccharide lytic enzymes of a marine isolate, Bacillus cereus NRC-20 and their application in biofilm removal

Sanaa M. Ashour
  • Sanaa M. Ashour
  • Botany Department, College of Women for Arts, Sciences and Education, Ain Shams University, Cairo, Egypt
  • Google Scholar
Zeinab M. H. Kheiralla
  • Zeinab M. H. Kheiralla
  • Botany Department, College of Women for Arts, Sciences and Education, Ain Shams University, Cairo, Egypt
  • Google Scholar
Ahmed I. Eldiwany*
  • Ahmed I. Eldiwany*
  • Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, Egypt
  • Google Scholar
Dina A. Maany
  • Dina A. Maany
  • Department of Chemistry of Natural and Microbial Products, National Research Centre, Cairo, Egypt
  • Google Scholar


  •  Received: 17 April 2012
  •  Accepted: 12 May 2014
  •  Published: 25 June 2014

References

Achten WMJ, Verchot L, Mathijs E,Singh VP, Aerts R, Muys B (2008). Jatropha bio diesel production and use. Biomass and Bioenergy. 32:1063-1084.
Crossref
 
Aldridge IY, Chmurny AB, Durham DR, Roberts RL, Fan LD (1994). Proteases to inhibit and remove biofilm. European patent, 0 590 746 A1.
 
Alexeeva YV, Ivanova EP, Bakunina IY, Zvayginsteva TN (2002).Optimization of glucosidase production by Pseudoalteromonas issachenkonii. Lett. Appl. Microbiol. 35(4):343-346.
Crossref
 
Byrd SM, Pang B, Hong W, Waligora AE, Juneau AR, Chelsie E, Parsek RM, Kock DN, Wozniak JD, Swords WE (2011). Direct evaluation of Pseudomanas aeruginosa biofilm mediators in a chronic infection model. J. Infect. Immun. 79:3087 3095.
Crossref
 
Chaignon P, Sadovskaya I, Ragunah CH, Ramasubbu N, Kaplan JB, Jabbouri S (2007). Susceptibility of staphylococcal biofilms to enzymatic treatments depends on their chemical composition. Appl. Microbiol. Biotechnol. 75:125-132.
Crossref
 
Chen X, Stewart PS (2000). Biofilm removal caused by chemical treatments. Water Res.34: 4229-4233.
Crossref
 
Christensen BE (1989). The role of extracellular polysaccharide in biofilms. J. Biotechnol. 10:181-202.
Crossref
 
Costerton JWZ, Lewandowski DE, Caldwell DR, Korber and Lappin-Scott HM (1995).Microbial biofilms. Annu. Rev. Microbiol. 49:711-745.
Crossref
 
da Re S, Ghigo JM (2006). A CsgD-independent pathway for cellulose Production and biofilm formation in Escherichia coli. J. Bacteriol. 188:3073-3087.
Crossref
 
de Carvalho CCCR (2007). Biofilms: recent developments on an old battle. Recent Patents Biotechnol. 1:49-57.
Crossref
 
de Queiroz G, Day DF(2007). Antimicrobial activity and effectiveness of a combination of sodium hypochlorite and hydrogen peroxide in killing and removing Pseudomonas aeruginosa biofilms from surfaces. J. Appl. Microbiol. 103(4):794-802.
Crossref
 
Donlan MR, Costerton JW (2002). Biofilms: Survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 167-193.
Crossref
 
Fernández L, Breidenstein EM, Song D, Hancock ER (2012). Role of intracellular proteases in the antibiotic resistance, motility and biofilm formation of Pseudomanas aeruginosa. J. Antimicrob. Agents. Chemother. 56:1128 1132.
Crossref
 
Flemming HC, Schaule G, McDonogh RG (1992).Biofouling on membranes. A short review, In. Melo LF, BottTR, FletcherM, Capdeville B. (ed.), Biofilms science and technology. Kluwer Academic Press, Dordrecht, The Netherlands. Pp. 487-497.
Crossref
 
Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B (2003). Microbial α amylases: A biotechnological perspective. J. Proc. Biochem. 38:1599 1616.
Crossref
 
Hansen BJ, Nakamura KL (1985). Distribution of alginate lyase enzyme activity among strains of Bacillus circulans. Appl. Environ. Microbiol. 122:1019-1021.
 
Hansen BJ, Scott Doubet R, Ram J (1984). Alginase enzyme production by Bacillus circulans. Appl. Environ. Microbiol. 47(4):704-9.
Pubmed
 
Häussler S, Parsek RM (2010). Biofilms, new perspectives at the heart of surface-associated microbial communities. J. Bacteriol. 192(12):2941-2949.
Crossref
 
Ivanova EP, Vysotskii MV, Svetashev VI, Nedashkovskaya OI, Gorshkova MN, Mikhailov VV, Yumoto N, Shigeri Y, Tagushi T, Yushikawa S (1999). Characterization of Bacillus strains of marine origin. Int. Microbiol. 2:267-271.
Pubmed
 
Jain S, Ohman DE (2005). Alginate secretion in Pseudomonas aeruginosa. Infect. Immun.73:6429-6436.
Crossref
 
Johansen C, Falholt P, Gram L (1997). Enzymatic removal and disinfection of bacterial biofilms. Appl. Environ. Microbiol. 63(9):3724-3728.
Pubmed
 
Johanson TB, Lindsey NS, Nelson CD, Mayo AJ (2008). Extracellular proteolytic activities expressed by Bacillus pumilus isolated from endodontic and periodontal lesions. J. Med. Microbiol.57:643-651.
Crossref
 
Kaplan JB (2010). Biofilm dispersal: Mechanisms, Clinical Implications, and Potential Therapeutic Uses. JDR89(3):205-218.
 
Lequette Y, Boels G, Clarisse M, Faille C (2010). Using enzymes to remove biofilms of bacterial isolates samples in the food industry. J. Biofoul. 4:421 431.
Crossref
 
Liu L, Chi-Li M (1988). Thermostable alginate lyase from Bacillus stearothermophilus. US patent 5139945.
 
Loiselle M, Anderson K (2003). The use of cellulose in inhibiting biofilm formation from organisms commonly found on medical implants. Biofoul. 2:77-85.
Crossref
 
Lopez D, Valmakis H, Kolter R (2010). Biofilms. Cold Spring Harb. Perspect. Biol.27 May, 2010.
Crossref
 
Lowry 0H, Rosenbrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
 
Manyak DM, Weiner RM, Carlson PS, Quintero EJ (2004). Preparation and use of biofilm degrading, multiple specificity enzyme mixture. US patent,6759040.
 
May BT, Shinabarger D, Maharaj R, Kato J, Chu L, De Vault DJ, Roychoudhury S, Zielinski AN, Berry A, Rothmel KR, Misra KT, Chakrabarty MA (1991). Alginate eynthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients. Clin. Microbiol. Rev. 4 (2):191 206.
Pubmed
 
Morikawa M, Kagihiro S, Haruki M, Tanako K, Branda S, Kolter R, Kanaya S (2006). Biofilm formation by a Bacillus subtilis strain that produces γ-glutamate. Microbiol. 152:2801-2807.
Crossref
 
Namasivayam E, Ravindar DJ, mariapan K, Jiji A, Kumar M, Jayaraj R (2011). Production of extracellular pectinase by Bacillus cereus isolated from market solid waste. J. Bioanal. Biomed. 3: 070-075.
 
Oldak E, Trafny AE (2005). Secretion of protease by Pseudomonas aeruginosa biofilm exposed to ciprofloxacin. Antimicrob. agents of chemo. 49(8):328-3288.
 
Orgaz B, Kives J, Pedregosa AM, Monistrol IF, Laborda F, SanJose C (2006). Bacterial biofilms removal using fungal enzymes. J. Enz. Microb. Technol. 40:51 56.
Crossref
 
Peterson SB, Dunn AK, Klimowicz AK, Handelsman J (2006). Peptidoglycan from Bacillus cereus mediates commensalism with rhizosphere bacteria from the Cytophaga-flavobacterium group. Appl. Environ. Microbiol. 72(8):5421-5427.
Crossref
 
Seidl K, Goerke C, Wolz C, Mack D, Berger-Bächi D, Bischoff M (2008). Staphylococcus aureus CcPA affects biofilm formation. Infect. Immun. 76 (5):2044 2050.
Crossref
 
Stoudt TH, Nollstadt KH (1982). Oral hygiene enzymes and methods for preparation. US patent 4335101.
 
Sumner JB, Sisler EB (1944). A simple method for blood sugar. Arch. Biochem. 4:333-336.
 
Sutherland IW (1999). Polysaccharases for microbial exopolysaccharides. Carb. Polymers. 38 (4):319-328.
 
Walker SL, Fougialakis M, Gerezo B, Livens S (2007). Removal of microbial biofilms from dispense equipment: the effect of enzymatic pre-digestion and detergent treatment. J. Inst. Brew. 113:61-66.
Crossref
 
Wang Y, Suzuki A, Tanaka T, Kumura H, Shimazaki K (2004). Partial characterization of dextrin degrading enzyme obtained from blue cheese. J. Dairy Sci. 87:1627-1633.
Crossref
 
Xavier JB, Picioreanu C, Abdul Rani S, Van Loosdricht MCM, Stewart SP (2005). Biofilm control strategies based on enzymic disruption of biofilm. A model study. Microbiol. 151:3817-3832.
Crossref
 
Xiong Y, Liu Y (2010). Biological control of microbial attachment: a promising alternative for mitigating membrane biofouling. Appl. Microbiol. Biotechnol. DOI10.1007.