African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Performance of dried blood spot (DBS) PUNCHER and dried blood spots to measure HIV-1 viral load

Anoumou Dagnra
  • Anoumou Dagnra
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar
Mounerou Salou
  • Mounerou Salou
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar
Amivi Ehlan
  • Amivi Ehlan
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar
Ahouefa Konou
  • Ahouefa Konou
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar
Alassane Ouro-Medeli
  • Alassane Ouro-Medeli
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar
Sika Dossim
  • Sika Dossim
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar
Komlan Ali-Edje
  • Komlan Ali-Edje
  • Laboratoire BIOLIM, Faculté des Sciences de la Santé, Université de Lomé, Lomé, Togo.
  • Google Scholar


  •  Received: 17 July 2016
  •  Accepted: 06 September 2016
  •  Published: 21 September 2016

References

Alvarez-Mu-oz MT, Zaragoza-Rodríguez S, Rojas-Montes O, Palacios-Saucedo G, Vázquez-Rosales G, Gómez-Delgado A, Torres J, Mu-oz O (2005). High correlation of human immunodeficiency virus type-1 viral load measured in dried-blood spot samples and in plasma under different storage conditions. Arch. Med. Res. 36(4):382-386.
Crossref

 

Arredondo M, Garrido C, Parkin N, Zahonero N, Bertagnolio S, Soriano V, de Mendoza C (2012). Comparison of HIV-1 RNA measurements obtained by using plasma and dried blood spots in the automated abbott real-time viral load assay. J. Clin. Microbiol. 50(3):569-572.
Crossref

 
 

Bertagnolio S, Parkin NT, Jordan M, Brooks J, García-Lerma JG (2010). Dried blood spots for HIV-1 drug resistance and viral load assaying: A review of current knowledge and WHO efforts for global HIV drug resistance surveillance. AIDS Rev. 12(4):195-208

 
 

Brambilla D, Jennings C, Aldrovandi G, Bremer J, Comeau AM, Cassol SA, Dickover R, Jackson JB, Pitt J, Sullivan JL, Butcher A, Grosso L, Reichelderfer P, Fiscus SA (2003). Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays for human immunodeficiency virus RNA: measurement, precision, and RNA stability. J. Clin. Microbiol . 41(5):1888-1893.
Crossref

 
 

Bland JM, DG (1986). Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1(8476):307-310.
Crossref

 
 

Cassol S, Gill MJ, Pilon R, Cormier M, Voigt RF, Willoughby B, Forbes J (1997). Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper. J. Clin. Microbiol. 35(11):2795-27801.

 
 

Garrido C, Zahonero N, Corral A, Arredondo M, Soriano V, de Mendoza C (2009). Correlation between human immunodeficiency virus type 1 (HIV-1) RNA measurements obtained with dried blood spots and those obtained with plasma by use of Nuclisens EasyQ HIV-1 and Abbott RealTime HIV load assays. J. Clin. Microbiol. 47(4):1031-1036
Crossref

 
 

Hamers RL, Smit PW, Stevens W, Schuurman R, Rinke de Wit TF (2009). Dried fluid spots for HIV type-1 viral load and resistance genotyping: a systematic review. Antivir. Ther. 14(5):619-629.

 
 

Johannessen A, Garrido C, Zahonero N, Sandvik L, Naman E, Kivuyo SL, Kasubi MJ, Gundersen SG, Bruun JN, de Mendoza C (2009). Dried blood spots perform well in viral load monitoring of patients who receive antiretroviral treatment in rural Tanzania. Clin. Infect. Dis. 49(6):976-981.
Crossref

 
 

Johannessen A, Garrido C, Zahonero N, Naman E, de Mendoza C (2011). HIV-1 drug resistance assaying from dried blood spots collected in rural Tanzania using the ViroSeq HIV-1 Genotyping System. J. Antimicrob. Chemother. 66(2):260-264
Crossref

 
 

Kane CT, Ndiaye HD, Diallo S, Ndiaye I, Wade AS, Diaw PA, Gaye-Diallo A, Mboup S (2008). Quantitation of HIV-1 RNA in dried blood spots by the real-time NucliSENS EasyQ HIV-1 assay in Senegal. J. Virol. Methods 148(1-2):291-295.
Crossref

 
 

Marconi A, Balestrieri M, Comastri G, Pulvirenti FR, Gennari W, Tagliazucchi S, Pecorari M, Borghi V, Marri D, Zazzi M (2009). Evaluation of the Abbott Real-Time HIV-1 quantitative assay with dried blood spot specimens. Clin. Microbiol. Infect. 15(1):93-97
Crossref

 
 

Mbida AD, Sosso S, Flori P, Saoudin H, Lawrence P, Monny-Lobé M, Oyono Y, Ndzi E, Cappelli G, Lucht F, Pozzetto B, Oukem-Boyer OO, Bourlet T (2009). Measure of viral load by using the Abbott Real-Time HIV-1 assay on dried blood and plasma spot specimens collected in 2 rural dispensaries in Cameroon. J. Acquir. Immune. Defic Syndr. 52(1):9-16
Crossref

 
 

Mellors JW, Mu-oz A, Giorgi JV, Margolick JB, Tassoni CJ, Gupta P, Kingsley LA, Todd JA, Saah AJ, Detels R, Phair JP, Rinaldo CR Jr (1997). Plasma viral load and CD4+ lymphocytes as prognostic markers of HIV-1 infection. Ann. Intern. Med. 126(12):946-954.
Crossref

 
 

Monleau M, Butel C, Delaporte E, Boillot F, Peeters M (2010). Effect of storage conditions of dried plasma and blood spots on HIV-1 RNA quantification and PCR amplification for drug resistance genotyping. J. Antimicrob. Chemother. 65(8):1562-1566.
Crossref

 
 

Neogi U, Gupta S, Rodridges R, Sahoo PN, Rao SD, Rewari BB, Shastri S, Costa AD, Shet A (2012). Dried blood spot HIV-1 RNA quantification: a useful tool for viral load monitoring among HIV-infected individuals in India. Indian J. Med. Res. 136(6):956-962.

 
 

WHO (2010). Technical Brief on HIV viral load technologies. Available at: View.

 
 

WHO (2010). Manel for HIV drug resistance testing using dried blood spot specimen, March 2010. Available at: 

View.

 
 

WHO (2012). Antiretroviral therapy for HIV infection in adults and adolescents : recommendations for public health approach.-2010 rev. Available at: 

View, accessed on June 13 2012

 
 

Yilmaz G (2001). Diagnosis of HIV infection and laboratory monitoring of its therapy. J. Clin. Virol. 21(3):187-196.
Crossref