African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method

Chikezie Ihebuzoaju Owuama
  • Chikezie Ihebuzoaju Owuama
  • Department of Microbiology, Modibbo Adama University of Technology, Yola, Nigeria.
  • Google Scholar


  •  Received: 28 March 2017
  •  Accepted: 31 May 2017
  •  Published: 21 June 2017

References

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Bradshaw LJ (1979). Laboratory manual. W.B. Saunders Company, London. 3rd edition.

 
 

Brown AM, Young PA (1947). A dilution method for the assay of streptomycin. Microbiology 1:353-360.
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CLSI (1998). Methods for Determining Bactericidal Activity of Antimicrobial Agents. Approved Guideline, NCCLS document M26-A. Clinical and Laboratory Standards Institute, 950 West Valley Roadn Suite 2500,Wayne, Pennsylvania 19087, USA.

 
 

CLSI (2012). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard, 9th ed., CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA.

 
 

Eucast (2003). Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clin. Microbiol. Infect. 6(9):509-515.

 
 

Lambert RJ, Pearson J (2000). Susceptibility testing: Accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values. J. Appl. Microbiol. 88(5):784-790.
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Owuama CI (2015). Microbiology Laboratory manual. Microtrend Digital Press, Yola. ISBN 978-978-943-328-2.

 
 

Wiegand I, Hilpert K, Hancock RE (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3(2):163-175.
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