African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Cloning and expression of a novel cry gene that is potentially active against nematodes

Janaína Fernandes Gonçalves
  • Janaína Fernandes Gonçalves
  • Departamento de Biologia Aplicada à Agropecuária, Univ Estadual Paulista, Via de Acesso Prof. Paulo D Castellane s/n, 14884-900, Jaboticabal-SP, Brazil
  • Search for this author on:
  • Google Scholar
Marcelo Luiz de Laia
  • Marcelo Luiz de Laia
  • Departamento de Tecnologia, Univ Estadual Paulista, Via de Acesso Prof. Paulo D Castellane s/n, 14884-900, Jaboticabal-SP, Brazil
  • Search for this author on:
  • Google Scholar
Bruno Flávio Figueiredo Barbosa
  • Bruno Flávio Figueiredo Barbosa
  • Departamento de Fitossanidade, Univ Estadual Paulista Via de Acesso Prof. Paulo D Castellane s/n Rural 14884-900 - Jaboticabal, SP - Brazil
  • Search for this author on:
  • Google Scholar
Vivian Boter Bergamasco
  • Vivian Boter Bergamasco
  • Departamento de Biologia Aplicada à Agropecuária, Univ Estadual Paulista, Via de Acesso Prof. Paulo D Castellane s/n, 14884-900, Jaboticabal-SP, Brazil
  • Search for this author on:
  • Google Scholar
Jaime Maia dos Santos
  • Jaime Maia dos Santos
  • Departamento de Fitossanidade, Univ Estadual Paulista Via de Acesso Prof. Paulo D Castellane s/n Rural 14884-900 - Jaboticabal, SP - Brazil
  • Search for this author on:
  • Google Scholar
Alisson Lucrecio da Costa
  • Alisson Lucrecio da Costa
  • Departamento de Ciência do Solo, Universidade Federal de Lavras, Brazil
  • Search for this author on:
  • Google Scholar
Manoel Victor Franco Lemos*
  • Manoel Victor Franco Lemos*
  • Departamento de Biologia Aplicada à Agropecuária, Univ Estadual Paulista, Via de Acesso Prof. Paulo D Castellane s/n, 14884-900, Jaboticabal-SP, Brazil
  • Search for this author on:
  • Google Scholar


  •  Accepted: 17 February 2014
  •  Published: 05 March 2014

References

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25:3389-3402.
http://dx.doi.org/10.1093/nar/25.17.3389
PMid:9254694 PMCid:PMC146917
 
Alves SB, Almeida JEM (1998). Desenvolvimento, potencial de uso e comercialização de produtos microbianos [Development, potential of use and commercialisation of microbial products]. In: Alves, S. B. (org.) Controle Microbiano de Insetos [Microbial control of insects]. 2a ed. Piracicaba: FEALQ, p. 1163.
 
Andrassy I (1992). A short census of free-living nematodes. Fund. Appl. Nematol. 15:187-188.
 
Atkinson HJ, Urwin PE, Mcpherson MJ (2003). Engineering plants for nematode resistance. Annu. Rev. Phytopathol. 41:615-639.
http://dx.doi.org/10.1146/annurev.phyto.41.052002.095737
PMid:12730388
 
Bates D, Maechler M, Bolker B, Walker S (2013). lme4: Linear mixed-effects models using Eigen and S4. R package version 1.0-5. http://CRAN.R-project.org/package=lme4.
 
Bergamasco VB, Gonçalves JF, Polanczyk RA, Desidério JA, Lemos MVF (2011). Expression of a new Bacillus thuringiensis cry1Ia gene in Escherichia coli with Strong Activity Against Cotton Pests. Aus. J. Basic Appl. Sci. 5:526-533.
 
Berón CM, Salerno GL (2007). Cloning and Characterization of a Novel Crystal Protein from a native Bacillus thuringiensis isolate highly active against Aedes aegypti. Curr. Microbiol. 54:271-276.
http://dx.doi.org/10.1007/s00284-006-0299-8
PMid:17334846
 
Bhalla R, Dalal M, Panguluri SK, Jagadish B, Mandaokar AD, Singh AK, Kumar PA (2005). Isolation, characterization and expression of a novel vegetative insecticidal protein gene of Bacillus thuringiensis. FEMS Microbiol. Lett. 243:467-472.
http://dx.doi.org/10.1016/j.femsle.2005.01.011
PMid:15686851
 
Birch ANE, Begg GS, Squire GR (2011). How agro-ecological research helps to address food security issues under new IPM and pesticide reduction policies for global crop production systems. J. Exp. Bot. 62(10):3251-3261.
http://dx.doi.org/10.1093/jxb/err064
PMid:21669880
 
Bobrowski VL, Fiúza, LM, Pasquali G, Bodanese-Zanettini MH (2003). Genes de Bacillus thuringiensis: uma estratégia para conferir resistência a insetos em plantas [Bacillus thuringiensis genes: an approach to confer insect resistance to plants]. Ciência Rural 34:843-850.
http://dx.doi.org/10.1590/S0103-84782003000500008
 
Bravo A, Gill SS, Soberón M (2007). Mode of action of Bacillus thuringiensis Cry and Cyt toxins and potential for insect control. Toxicon. 49:423-435.
http://dx.doi.org/10.1016/j.toxicon.2006.11.022
PMid:17198720 PMCid:PMC1857359
 
Brodie BB (1971). Differential vertical movement of non volatile nematicides in soil. J. Nematol. 3:292-295.
PMid:19322385 PMCid:PMC2619877
 
Brown CR, Mojtahedi H, Santo, GS (1991). Resistance to Columbia root-knot nematode in Solanum ssp. and in hybrids of S. hougasii with tetraploid cultivated potato. Am. J. Potato 68:445-452.
http://dx.doi.org/10.1007/BF02853782
 
Bukhari DAA, Shakoori AR (2009). Cloning and expression of Bacillus thuringiensis cry11 crystal protein gene in Escherichia coli. Mol. Biol. Rep. 36:1661-1670.
http://dx.doi.org/10.1007/s11033-008-9366-5
PMid:18821029
 
Bulla LA Jr, Bechtel DB, Kramer KJ, Shethna YI, Aronson AI, Fitz-James PC (1980). Ultrastructure, physiology and biochemistry of Bacillus thuringiensis. Cr. Rev. Microbiol. 8:147-204.
http://dx.doi.org/10.3109/10408418009081124
PMid:7000441
 
Capalbo DMF, Vilas-Boas GFLT, Arantes OMN, Suzuki M (2005). Bacillus thuringiensis: formulações e plantas transgênicas [Bacillus thuringiensis: formulations and transgenic plants]. Biotecnologia ciência & desenvolvimento, Editora Folha de Viçosa, Brasil 34:76-83.
 
Chak KF, Tseng MY, Yamamoto T (1994). Expression of the crystal protein gene under the control of the a-amylase promoter in Bacillus thuringiensis strains. Appl. Environ. Microbiol. 60:2304-2310.
PMid:8074511 PMCid:PMC201647
 
Crickmore N, Baum J, Bravo A, Lereclus D, Narva K, Sampson K, Schnepf E, Sun M, Zeigler DR (2013). Bacillus thuringiensis toxin nomenclature. Available at: <http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/ Accessed on: 2014.
 
Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van rie J, Lereclus D, Baum J, Dean D (1998). Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:807-813.
PMid:9729610 PMCid:PMC98935
 
Ewing B, Green P (1998). Basecalling of automated sequencer traces using phred II Error probabilities. Genome Res. 8:186-194.
http://dx.doi.org/10.1101/gr.8.3.175
PMid:9521922
 
Ewing B, Hillier L, Wendl M, Green P (1998). Basecalling of automated sequencer traces using phred I Accuracy assessment. Genome Res. 8:175-185.
http://dx.doi.org/10.1101/gr.8.3.175
PMid:9521921
 
Freitas LG, Neves WD, Marra BM, Almeida AMS, Romeiro RS (2005). Isolamento e seleção de rizobactérias para o controle do nematoide formadores de galhas (Meloidogyne spp.) na cultura do tomateiro. Nematol. Bras. 2:215-220.
 
Glare TR, O'callagham M (2000). Bacillus thuringiensis: biology, ecology and safety. Chichester: John Wiley and Sons p. 350.
 
Gonçalves JF, Laia ML, Cordeiro JX, Lemos MVF (2009). Identification of new cry genes in Bacillus thuringiensis isolates by multiplex PCR and RFLP. Res. J. Agric. Biol. Sci. 5:326-337.
 
Gordon D, Abajian C, Green P (1998). Consed: a graphical tool for sequence finishing. Genome Res. 8:195-202.
http://dx.doi.org/10.1101/gr.8.3.195
PMid:9521923
 
Gould F, Blair N, Reid M, Rennie TL, Lopez J, Micinski S (2002). Bacillus thuringiensis-toxin resistance management: Stable isotope assessment of alternate host use by Helicoverpa zea. Proc. Natl. Acad. Sci. 99:16581-16586.
http://dx.doi.org/10.1073/pnas.242382499
PMid:12464681 PMCid:PMC139186
 
Griffitts JS, Whitacre JL, Stevens DE, Aroian RV (2001). Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme. Science 293:860-864.
http://dx.doi.org/10.1126/science.1062441
PMid:11486087
 
Hardin SH, Jones LB, Homayouni R, McCollum JC (1996). Octamer - primed Cycle Sequencing: Design of an Optimized Primer Library. Genome Methods 6:545-550.
 
Hoss S, Arndt M, Baumgarte S, Tebbe C.C, Nguyen HT, Jehle JA (2008). Effects of transgenic corn and Cry1Ab protein on the nematode, Caenorhabditis elegans. Ecotox. Environ. Safe 70 334-340
http://dx.doi.org/10.1016/j.ecoenv.2007.10.017
PMid:18068780
 
Huang Z, Guan C, Guan X (2004). Cloning, characterization and expression of a new cry1Ab gene from Bacillus thuringiensis WB9. Biotechnol. Lett. 26:1557-1561.
http://dx.doi.org/10.1023/B:BILE.0000045652.00137.1f
PMid:15604796
 
Huehne PS, Lertwiriyawong B, Chanpaisaeng J (2005). Cloning of Bacillus thuringiensis cryIE(a) gene effecting Spodoptera exigua in Thailand. Nucleotide sequence from EMBL/GenBank/DDBJ databases. <https://www.ncbi.nlm.nih.gov/nuccore/GI:142751>
 
Jenkins W R (1964). A rapid centrifugal flotation technique for separating nematodes from soil. Plant Dis. Rep. 48:692-695.
 
Kao SS, Hsieh FC, Tzeng CC, Tsai YS (2003). Cloning and expression of the insecticidal crystal protein gene Cry1Ca9 of Bacillus thuringiensis G10-01A from Taiwan granaries. Curr. Microbiol. 47:295-299.
http://dx.doi.org/10.1007/s00284-002-3982-4
PMid:14629010
 
Karim S, Riazuddin S, Gould F, Dean DH (2000). Determination of receptor binding properties of Bacillus thuringiensis d-endotoxins to cotton bollworm (Helicoverpa zea) and pink bollworm (Pectinophora gossypiella) midgut brush border membrane vesicles. Pestic. Biochem. Physiol. 67:198-216.
http://dx.doi.org/10.1006/pest.2000.2491
 
Kostova M, Naimov S, Dukiandjiev S, Minkov I (2008). Molecular characterization of Bulgarian Bacillus thuringiensis isolates. C. R. Acad. Bulg. Des Sci. 61: 475-480.
 
Kumar S, Birah A, Chaudhary B, Burma PK, Gupta GP, PentaL D (2005). Plant codon optimized cry genes of Bacillus thuringiensis can be expressed as soluble proteins in Escherichia coli BL21 Codon Plus strain as NusA-Cry protein fusions. J. Invertebr. Pathol. 88:83-86.
http://dx.doi.org/10.1016/j.jip.2004.09.002
PMid:15707873
 
Kumar S, UDAYasuriyan V (2004). Cloning of cry2Aa and cry2Ab genes from new isolates of Bacillus thuringiensis and their expression in recombinant Bacillus thuringiensis and Escherichia coli strains. World J. Microbiol. Biotechnol. 20:11-17.
http://dx.doi.org/10.1023/B:WIBI.0000013285.15036.0d
 
Li XQ, Wei JZ, Tan A, Aroian RV (2007). Resistance to root-knot nematode in tomato roots expressing a nematicidal Bacillus thuringiensis crystal protein. Plant Biotechnol. pp. 455-464.
http://dx.doi.org/10.1111/j.1467-7652.2007.00257.x
PMid:17451491
 
Bravo A, Likitvivatanavong S, Gill SS, Soberón M (2011). Bacillus thuringiensis: A story of a successful bioinsecticide Insect Biochem Mol Biol. 41: 423 - 431.
http://dx.doi.org/10.1016/j.ibmb.2011.02.006
PMid:21376122 PMCid:PMC3689885
 
Lucas JA (2011). Advances in plant disease and pest management. J. Agr. Sci. 149: 91-114.
http://dx.doi.org/10.1017/S0021859610000997
 
Luo H, Xiong J, Zhou Q, Xia L, Yu Z (2013). The effects of Bacillus thuringiensis Cry6A on the survival, growth, reproduction, locomotion, and behavioral response of Caenorhabditis elegans. Appl. Microbiol. Biotechnol. 97(23):10135-10142
http://dx.doi.org/10.1007/s00253-013-5249-3
PMid:24100681
 
Marroquin LD, Elyassnia D, Griffitts JS, Feitelson JS, Aroian R (2000). Bacillus thuringiensis (Bt) Toxin Susceptibility and Isolation of Resistance Mutants in the Nematode Caenorhabditis elegans. Genetics 155:1693-1699.
PMid:10924467 PMCid:PMC1461216
 
Moar WJ, Trumble JT, Hice RH, Backman PA (1994). Insecticidal Activity of the CryllA Protein from the NRD-12 Isolate of Bacillus thuringiensis subsp. kurstaki Expressed in Escherichia coli and Bacillus thuringiensis and in a Leaf- Colonizing Strain of Bacillus cereus. Appl. Environ. Microbiol. 60:896-902.
PMid:8161182 PMCid:PMC201407
 
Polanczyk RA, Valicente F H, Barreto MR (2008). Utilização de Bacillus thuringiensis no controle de pragas agrícolas na América Latina [The use of Bacillus thuringiensis in the control of agricultural pests in Latin America]. In S.B. Alves SB, Lopes RB Controle microbiano de pragas na América Latina: avanços e desafios [Microbial control of pests in Latin America: advances and challenges]. Piracicaba, FEALQ, Chapter 4: 111-136.
 
Porcar M, Juarez-Perez V (2003). PCR-based identification of Bacillus thuringiensis pesticidal crystal genes. Fems Microbiol. Rev. 26: 419-432.
http://dx.doi.org/10.1111/j.1574-6976.2003.tb00624.x
PMid:12586389
 
Rozen S, Skaletsky HJ (2000). Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp. 365-386 Source code available at http://fokker.wi.mit.edu/primer3/. http://frodo.wi.mit.edu/primer3/.
 
Sambrook J, Russell D W (2001). Molecular Cloning: A Laboratory Manual. NY. 3.ed.
 
Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P (2011). Bacillus thuringiensis a century of research, development and commercial applications. Plant. Biotechnol. J. 9:283-300
http://dx.doi.org/10.1111/j.1467-7652.2011.00595.x
PMid:21375687
 
Sauka HD, Cozzi GJ, Benintende BG (2005). Screening of cry2 genes in Bacillus thuringiensis isolates from Argentina. Anton. van Leeuwen. 88:163-165.
http://dx.doi.org/10.1007/s10482-005-3368-2
PMid:16096692
 
Sayyed RZ, Patel PR (2011). Biocontrol Potential of Siderophore Producing Heavy Metal Resistant Alcaligenes sp. and Pseudomonas aeruginosa RZS3 vis-a`-vis Organophosphorus Fungicide. Indian J. Microbiol. 51(3):266-272
http://dx.doi.org/10.1007/s12088-011-0170-x
PMid:22754001 PMCid:PMC3209905
 
Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998). Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62: 775 - 806.
PMid:9729609 PMCid:PMC98934
 
Shi Y, Ma W, Yuan M, Sun F, Pang Y (2007). Cloning of vip1/vip2 genes and expression of Vip1Ca/Vip2Ac proteins in Bacillus thuringiensis. W. J. Microbiol. Biotechnol. 23:501-507.
http://dx.doi.org/10.1007/s11274-006-9252-z
 
Silva AR, Santos JM (2007). Nematoides na Cultura da Batata no Brasil. 1. ed. Itapetininga: Associação Brasileira da Batata, p. 55
 
Starr JL, Cook R, Bridge J (2002). Plant resistance to parasitic nematodes. Wallingford: CABI, 258 p.
http://dx.doi.org/10.1079/9780851994666.0000
 
Sun M, Liu Z, Yu Z (1999). Crystal protein gene cry032 active against plant parasitic nematodes from Bacillus thuringiensis YBT-032. Nucleotide sequence from EMBL/GenBank/DDBJ databases.<http://www.ncbi.nlm.nih.gov/nuccore/GI:17979618>
 
Sun Y, Wei W, Ding X, Xia L, Yuan Z (2007). Detection of chromosomally located and plasmid-borne genes on 20 kb DNA fragments in parasporal crystals from Bacillus thuringiensis. Arch. Microbiol. 188:327-332.
http://dx.doi.org/10.1007/s00203-007-0252-7
PMid:17516045
 
Tabashnik BE, Gassmann AJ, Crowder DW, Carrière Y (2008). Insect resistance to B.t crops: evidence versus theory. Nat. Biotechnol. 26:199-202.
http://dx.doi.org/10.1038/nbt1382
PMid:18259177
 
Zhang LL, Lin J, Luo L, Guan CY, Zhang Q L, Zhang Y, Huang ZP, Guan X (2007). A novel Bacillus thuringiensis strain LLB6, isolated from bryophytes, and its new cry2Ac-type gene. Lett. Appl. Microbiol. 44:301 - 307.
http://dx.doi.org/10.1111/j.1472-765X.2006.02072.x
PMid:17309508
 
Zheng D, Cruz N, Armengol G, Sevrez C, Olaya J, Yuan Z, Orduz S, Crickmore N (2007). Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis Subsp. israelensis in Asticcacaulis excentricus. Curr. Microbiol. 54:58-62.
http://dx.doi.org/10.1007/s00284-006-0352-7
PMid:17160360
 
Wei JZ, Hale K, Carta L, Platzer E, Wong CT, Fang SCT, Aroian RV (2003). Bacillus thuringiensis crystal proteins that target nematodes. PNAS 100(5):2760-2765.
http://dx.doi.org/10.1073/pnas.0538072100
PMid:12598644 PMCid:PMC151414
 
Wickham H (2009). ggplot2: elegant graphics for data analysis. Springer New York.
 
Wilson K (1987) Large Scale CsCl Preparation of Bacterial Genomic DNA. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds.). Cur. Prot. Mol. Biol. ISBN: 047150338X. Greene Publishing Associates and Wiley-Interscience. pp:243-245.
 
Wu Y, Lei CF, Yi D, Liu PM, Gao MY (2011). Novel Bacillus thuringiensis δ-Endotoxin Active against Locusta migratoria manilensis. Appl. Environ. Microbiol. 77(10):3227-3233.
http://dx.doi.org/10.1128/AEM.02462-10
PMid:21441319 PMCid:PMC3126473

 

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 10
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES