African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5188

Table of Content: 4 May; 4(9)

May 2010

Development and evaluation of colloidal gold immunochromatographic strip for detection of Escherichia coli O157

  Escherichia coli O157:H7 is a serious and common human pathogen that can cause diarrhoea, haemorrhagic colitis, and haemolytic uraemic syndrome (HUS). In this study, the synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates probe specific to E. coli O157:H7 were performed, and the preparation of colloidal gold immunochromatographic strip based the...

Author(s):   Xihong Zhao, Xiaowei He, Wenmei Li , Yao Liu, Liansheng Yangand Jihua Wang  

May 2010

Efficiency of filtration technique for isolation of leptospires from surface waters: Role of different membranes with different pore size and materials

  The objective of this research focused on evaluation of membrane filters application for isolation of Leptospira from surface water samples. The filter materials evaluated included nitrocellulose (0.22 and 0.45 µm pore diameters), polyvinylidene fluoride (Durapore 0.22 and 0.40 μm pore diameters), nylon mesh (37 μm), and glass fiber (1.0 µm). Millipore polyvinylidene fluoride...

Author(s):   Hami Kaboosi, Mohammad Reza Razavi, and Ashraf al Sadat Noohi  

May 2010

Antibiotic resistance, integrons and Salmonella genomic island 1 among Salmonella Schwarzengrund in broiler chicken and pig

  Salmonella Schwarzengrund is an infrequent serovar isolated from humans in Taiwan. However, this serovar is which is highly invasive, is found in humans as report by two independent epidemiologic surveys. The present study delineated the widespread resistance to fluoroquinolone in S. Schwarzengrund isolated from both poultry and swine carcesses in Taiwan. We conducted the present study to...

Author(s): Yu-Chih Wang, Yi-Chih Chang, Hsiao-Li Chuang, Chien-Chao Chiu, Kuang-Sheng Yeh, Chao-Chin Chang, Shih-Ling Hsuan and Ter-Hsin Chen,

May 2010

Effects of Rhizobium innoculation, lime and molybdenum on nitrogen fixation of nodulated Phaseolus vulgaris L.

  A field and glass house experiment was conducted with the aim of evaluatingRhizobium inoculation, molybdenum (Mo) and lime supply on growth and nitrogen fixation of nodulated Phaseolus vulgaris. The experiment was laid in a split-split plot design. The experimental treatments consisted of 2 levels ofRhizobium inoculation (with rhizobia and without rhizobia) 3 levels of Mo (0, 6 and 12 g...

Author(s): Sylvie Bambara and Patrick A. Ndakidemi

May 2010

The identification of the first isolate of influenza B virus using a duplex RT-PCR DNA sequencing in Saudi Arabia (B/Riyadh/01/2007)

  Although Influenza virus infections threaten thousands of lives each year worldwide and bear the risk of been an epidemic, little is known about the circulating strains in Saudi Arabia. The availability of a rapid and accurate diagnostic approach is essential for effective treatment and disease control. In the current study, we optimized a duplex RT-PCR assay for the simultaneous detection of...

Author(s): Fahad N. Almajhdi

May 2010

Antibiotic sensitivity of human genital mycoplasmas

  The therapeutic failure of antimycoplasma drugs, subject to resistance is vital for specific microbiological investigation to overcome urogenital diseases among women. The susceptibility of 5 Mycoplasma hominis strains and 11 strains ofUreaplasma urealyticum were studied against six antibiotics namely: minocycline, lincomycin, erythromycin, tetracycline, doxycycline and rosaramicin....

Author(s): Jafar Khan, Robeena Farzand and Pir Bux Ghumro

May 2010

Detection of adhesin genes and slime production among Staphylococci in orthopaedic surgical wounds

This study was aimed at investigating: (i) three adhesin genes (clf A, fnb A andcna) in Staphylococus aureus strains, (ii) the presence of slime (ica A and ica D genes) in both Staphylococus epidermidis and S. aureus strains isolated from surgical wounds. The slime and adhesin genes were detected by multiplex PCR. The ica A/ica D positivity rates...

Author(s): Nizami Duran, Yunus Dogramaci, Burcin Ozer, Cemil Demir and Aydiner Kalaci

May 2010

Determination of microbiological characteristics of Turkish Karin Kaymagi cheeses packaged in different materials

  The aim of this study is to transport to dairy plants the making technique of Karin Kaymagi cheese and to make them hygienic cheese. Also, the effects of different packaging materials (artificial case, barrel and tripe) on microorganism counts in the cheese samples were determined. In this research, four different Karin Kaymagi cheese samples were prepared from white cheese, civil cheese, whey cheese,...

Author(s): Salih Özdemir, Filiz Yangılar and Cihat Ozdemir

May 2010

Evaluation of salivary gland scintigraphy, magnetic resonance and diffusion-weighted imaging in clinical diagnosis of Sjögren’s Syndrome

  To assess diagnostic effectiveness of Sjögren’s syndrome (SS), salivary gland scintigraphy (SGS), magnetic resonance (MR) and diffusion-weighted imaging (DWI) were performed in 54 patients (primary SS, secondary SS and connective tissue disease (CTD) but without SS). Results showed that SGS and diffusion-weighted magnetic resonance imaging (MRI) provided high-resolution images of the...

Author(s): Mei Li, Hao-Jie Dai, Zhen-Chang Wang, Zhen-Gang Wang, Li Cui, Qing-Gang Xu and Jing Li

May 2010

Emulsifying bacteria in produce water from Niger-Delta, Nigeria

  The study reports the diversity of emulsifying bacteria in produce water obtained from Bonny Oil Terminal, Niger Delta, Nigeria. Physico–chemical analysis of produce water showed a high chloride ion content of 650.0 mg/L. Total aerobic mesophilic bacterial count was 8.6 x 106 CFU/mL while the oil utilizing bacterial count was 1.32 x 106 CFU/mL. The predominant bacteria genera...

Author(s): S. A. Balogun and O. E. Fagade

May 2010

Preliminary studies on Piliostigma thonningii Schum leaf extract: Phytochemical screening and in vitro antimalarial activity

  Different parts of Piliostigma thonningii Scum (Caesalpinioideae) have been used medicinally. The roots and twigs have been used locally in the treatment of dysentery, fever, respiratory ailments, snake bites, hookworm and skin infections in eastern Nigeria. The leaf extracts has been used for various ethnomedicinal purposes including the treatment of malaria all over eastern Nigeria....

Author(s): A. Kwaji, P. U. Bassi, M. Aoill, C. M. Nneji and G. Ademowo

May 2010

Systematic position of Moesziomyces penicillariae among Ustilaginaceae

  We revisited the systematic position of the fungus Moesziomyces penicillariae, the causal agent of smut of pearl millet, by using morphological characters, germination pattern of teliospores and molecular analysis of ribosomal sequences. Samples of smutted ears of pearl millet were harvested in Senegal (West Africa). Compared to the description of Moesziomyces genus sensus Vánky,our...

Author(s): Gnagna Diagne-Leye, Teresa Almaraz-Lopez, Maïmouna Sy-Ndir, Gérard Mascarell, Amadou Tidiane Ba and Christophe Roux,

May 2010

Antimicrobial properties of chilli lipoxygenase products

  Plant oxylipins, the metabolic products of polyunsaturated fatty acids have been variously implicated in control of microbial pathogens. To get a better insight into the biological activities of oxylipins, in vitro growth inhibition assays were used to investigate the direct antimicrobial activities of 9 and 13-lipoxygenase (LOX) products against a set of plant pathogenic bacteria and fungi. LOX...

Author(s): A. Sucharitha and P. Uma Maheswari Devi

May 2010

Cloning, expression, purification and characterization of the carboxylesterase Yeig from Escherichia coli k12

  In this work, enzyme YeiG from Escherichia coli K12-MG1655 which has no definitely known biochemical function has been cloned, expressed, purified and characterized. Alignment studies show that YeiG has an alpha/beta-hydrolase fold with catalytic triad formed by Ser145, Asp223 and His256 at active sites and Ser145 is located in the conserved motif Gly–Xaa–Ser–Xaa–Gly....

Author(s): Jie Lu, Xiao-Lin Jiang, Zhen Li, Hong-Fen Luan, Hong Chen, Xiu-Juan Wang and Chi-Bun Ching

May 2010

A survey on epidemiology of urinary tract infections and resistance pattern of uropathogens in an Iranian 1000-bed tertiary care hospital

  Urinary tract infections (UTIs) are one of the most common infectious diseases diagnosed in communities and hospitalized patients. The aim of this study was to determine frequency of occurrence and antimicrobial susceptibility patterns of uropathogens in Milad hospital of Tehran, Iran. In a prospective study from March to June 2009, a total of 11308 urine sample from patients admitted in Milad hospital of...

Author(s): Ava Behrooozi, Mohammad Rahbar  and Jalil Vand Yousefi

May 2010

Penicillium verruculosum RS7PF: A root fungal endophyte associated with an ethno-medicinal plant of the indigenous tribes of Eastern India

  Potentilla fulgens L., an ethno-medicinal plant of the ethnic tribes of Meghalaya in the Eastern Himalayan range of India was screened for root fungal endophytes. This plant is distributed in the wild habitats and in the traditionally preserved ‘Sacred Groves’ of Meghalaya in Eastern India, which is of late getting over-exploited for its medicinal value. Isolation of endophytes in water agar...

Author(s): R. K. Bhagobaty, S. R. Joshi and R. Kumar

May 2010

Comparison of PCR and BANA hydrolysis in detecting oral anaerobes in subgingival plaque

  Culture has always been recognized as the gold standard for detecting oral anaerobes in dental plaque samples. However, many of the bacterial morphotypes observed by microscopy are difficult to culture, thus necessitating the need for alternative methods of detection. The objective of this study was to evaluate the sensitivity and specificity of BANA (N-benzoyl-DL-arginine-B-naphthylamide) and PCR (Polymerase...

Author(s): Claude Bayingana, Ashley Pretorius and Charlene W. J. Africa

May 2010

Application of PCR-RFLP of gap gene method as a molecular typing tool for coagulase negative Staphylococci from bovine and human origin identified with VITEK 2

  The aim of this study was to apply the Restriction Fragment Length Polymorphism (RFLP) of Glyceraldehyde-3-Phosphate Dehydrogenase encoding gene (gap) for testing its performance as a molecular typing tool in coagulase negative staphylococci (CNS) isolates from bovine mastitis (n = 59) and human clinical cases (n = 13) identified with VITEK 2. According to the phenotypic identification results, bovine...

Author(s): Emel Banu Buyukunal Bal, Mehmet Ali Bal, Taner Isevi and Erkan Yula

May 2010

Screening, identification and statistic optimization of a novel exopolysaccharide producing Lactobacillus paracasei HCT

  The exopolysaccharides (EPS) producing ability was evaluated by phenol-sulphuric acid method and the Lactobacillus HCT was the highest yield EPS-producing strain, which was isolated from feces of Bama centenarian in Guangxi of China. It was identified as Lactobacillus paracasei HCT with carbohydrate assimilation profiling and 16S rRNA gene sequencing. L. paracasei HCT was found...

Author(s): Rihua Xu, Shimin Ma, Yang Wang, Lisha Liu  and Pinglan Li

May 2010

Observation on the occurrence and transmission pattern of Salmonella gallinarum in commercial poultry farms in Ogun State, South Western Nigeria

  Reports of heavy mortality in three commercial layer poultry farms and two meat-type turkey farms in Ogun State were received separately between March and December, 2008 at the Veterinary Teaching Hospital (VTH), University of Agriculture, Abeokuta, Nigeria. We investigated the cause of mortality, isolated and serotyped the aetiological agent, and carried out antimicrobial susceptibility testing. All five...

Author(s): Agbaje M, Davies R, Oyekunle M. A, Ojo O. E, Fasina F. O and Akinduti P. A

May 2010

The Copepod parasite of the gills of four teleost fishes caught from the gulf of Annaba (Algeria)

  The examination of the gills of 960 fishes belonging to four species: Diplodus annularis, Pagellus erythrinus, Lithognatus mormyrus and Mullus barbatuscollected from the Gulf of Annaba, enabled us to identify 13 species of parasitic copepods: Caligus ligusticus, Caligus diaphanus, Clavellotis sp, Clavellotis sargi, Clavellotis pagri, Clavellotis...

Author(s): Chahinez Boualleg, Hadda Ferhati, Nouha Kaouachi, Mourad Bensouilah and Sonia Ternengo

May 2010

Comparison of extract bio-activities of in- situ and vitro grown selected bryophyte species

  The antimicrobial activity of DMSO extracts of three bryophyte species, two mosses and a liverwort (Atrichum undulatum (Hedw.) P. Beauv., Marchantia polymorpha L. ssp. ruderalis Bischl. and Boisselier, Physcomitrella patens (Hedw.) Bruch and Schimp.) grown in nature and in axenic culture was evaluated by microdilution method against eight bacterial species (Escherichia...

Author(s): Aneta Sabovljević, Marina Soković, Jasmina Glamočlija, Ana Ćirić, Milorad Vujičić, Boris Pejin and Marko Sabovljević

May 2010

Creation of alternative energy by bio-ethanol production from pineapple waste and the usage of its properties for engine

  The world is facing problems of fossil fuels depletion. The problem of using fossil fuels can have an environmental impact due to the oxidation of CO2, SO2 and NOX emission. In order to overcome those problems, there is an alternative fuel that can replace fossil fuel such as bioethanol and biodiesel. Bioethanol can be derived from biomass with many different varieties of feedstocks such as...

Author(s): A. B. M. S. Hossain and A. R. Fazliny

May 2010

Isolation and molecular characterization of phosphate solubilizing Enterobacter and Exiguobacterium species from paddy fields of Eastern Uttar Pradesh, India

  Six phosphate solubilizing bacteria (PSB) were isolated from paddy fields of Eastern Uttar Pradesh, India harboring low available phosphorus. Taxonomic delineation employing morphological, biochemical, 16S rRNA gene sequences and phylogenetic affiliations suggests that they are members of Enterobacter andExiguobacterium genera. Of the six isolates, Enterobacter sp. LCR1 and...

Author(s):   Arvind Kumar, Poonam Bhargava and Lal Chand Rai  

May 2010

Mitochondrion activity and dispersal of Aspergillus fumigatus and Rhizopus oryzae

  We investigated the effects of anti-inflammatory and anti-mitochondrial compounds on spore dispersal in the pathogens, Aspergillus fumigatus andRhizopus oryzae. When acetylsalicylic acid (ASA) and other non steroidal anti-inflammatory drugs (NSAIDs) were added to bio-assays of A. fumigatus and R. oryzae, spore-releasing structures were targeted first at lower concentrations....

Author(s): Desmond M. Ncango, Chantel W. Swart, Carolina H. Pohl, Pieter W. J. Van Wyk and Johan L. F. Kock  

May 2010

The potential influence of high cholesterol diet-induced oxidative stress on composition and properties of red blood cells in rabbits

  Atherosclerosis and heart diseases are major causes of morbidity and mortality in adults in industrialized nations. The aim of this study was to assess the potential influence of high-cholesterol diet-induced oxidative stress on composition and properties of red blood cells (RBCs) in rabbits. Thus, percentage of hematocrit, RBCs, white blood cells (WBCs) and platelets counts, total cholesterol (TC), low...

Author(s):   Mohamed Anwar K. Abdelhalim  

May 2010

Staphylococcal cassette chromosome mec (SCCmec) analysis and antimicrobial susceptibility profiles of methicillin-resistant staphylococcus aureus (MRSA) isolates in a teaching hospital, Shantou, China

  Methicillin-resistant staphylococcus aureus (MRSA) infection has now become a major public health concern. The aim of this study is to determine the antibiotic resistance pattern and prevalence of different staphylococcal cassette chromosome mec (SCCmec) types among the MRSA isolates from a teaching hospital in Shantou, China. The minimum inhibitory concentrations (MICs) ofseventeen antimicrobial...

Author(s): Qing Peng, Bing Hou, Shuqin Zhou, Yuanchun Huang, Dexing Hua, Fen Yao and Yuan shu Qian

May 2010

Occurrence of tetracycline-resistant Aeromonas hydrophila infection in Korean cyprinid loach (Misgurnus anguillicaudatus)

  In this paper, we described a mass mortality of cyprinid loaches, Misgurnus anguillicaudatus from a private fish farm in Korea. Diffuse bleeding was observed on the skin around the anal orifice. Bacterial pathogens from kidneys samples of moribund fish were cultured, identified and confirmed to be Aeromonas hydrophila using morphological, biochemical test and genetical analysis. The...

Author(s): Jin Woo Jun, Ji Hyung Kim, Dennis K. Gomez, Casiano H. Choresca Jr, Jee Eun Han, Sang Phil Shin and Se Chang Park

May 2010

Isolation and characterization of Streptomyces species with antifungal activity from selected national parks in Kenya

  Microorganisms and their natural products are potentially important for the biological control of crop diseases without detrimental effects to the environment. In this study, acetonitrile-methanol extracts of 361 actinobacterial isolates obtained from Aberdares, Arabuko Sokoke, Lake Bogoria, Mt Kenya, Kakamega, Ruma, Shimba Hills and Imenti forest national parks in Kenya were screened for antagonism...

Author(s): James Oluoch Nonoh, Wilber Lwande, Daniel Masiga, Rafi Herrmann, James K. Presnail, Eric Schepers, Matilda Angela Okech, Richard Bagine, Paul Mungai, Aggrey Bernard Nyende and Hamadi Iddi Boga

May 2010

Demineralized bone matrix gelatin as scaffold for tissue engineering

  The aim of the present study was to do a comparative light microscopical and histochemical analysis of bone regeneration processes in rabbits, with the use of demineralized bone matrix gelatin scaffolds. Our histological results showed that no gelatin scaffold was extruded, indicating that neither body fluid nor blood swept the gelatin scaffold from the implantation site, nor was the gelatin scaffold...

Author(s): Yong Pan, Shiwu Dong, Yong Hao, Tongwei Chu, Changqing Li, Zhengfeng Zhang and Yue Zhou

May 2010

Differentiation of Salmonella enterica based on PCR detection of selected somatic and flagellar antigens

  Serotyping is the basis of Salmonella surveillance. However, the limitations of the traditional serotyping have stimulated rapid research and development in DNA-based serotyping. The aim of this study was to apply a combination of sequential multiplex PCRs targeting the O, H and Vi antigens to serotype a panel of 122 recently isolated human- and foodborne- Salmonella strains. The mPCR that...

Author(s): Mohd Elbagir Elhassan Nori and Kwai Lin Thong

May 2010

Effect of blood group and demographic characteristics on malaria infection, oxidative stress and haemoglobin levels in south western Nigeria

  Malaria infection generates oxidative stress which has serious effect on the haemoglobin (Hb) level of the infected individuals. This work studied the effect of blood group, age and gender on prevalence of malaria, oxidative stress and Hb level. 5 ml of blood samples were collected into EDTA bottle from 120 volunteered adult males and non-pregnant females. The plasma was separated and used to quantify...

Author(s): O.M. Akanbi , J.A. Badaki , O.Y. Adeniran , and O.O. Olotu 

May 2010

Frequency of extended spectrum beta-lactamase (ESBLs) producing Escherichia coli and klebseilla pneumonia isolated from urine in an Iranian 1000-bed tertiary care hospital

  Betal-lactam antibiotics are the most frequently prescribed antibiotics. Emerging resistance to these antibiotics among gram-negative bacilli limited their utility. This study was done to determine the frequency of extended spectrum beta-lactmase (ESBLs) producing Escherichia coli and Klebsiella pneumoniae isolated from urine in Milad Hospital of Tehran. This study was done...

Author(s): Ava Behrooozi, Mohammad Rahbar and Jalil Vand Yousefi

May 2010

Antimicrobial activity of the lichen Lecanora frustulosa and Parmeliopsis hyperopta and their divaricatic acid and zeorin constituents

  Antibacterial and antifungal activity of the acetone, methanol and aqueous extracts of the lichen Lecanora frustulosa and Parmeliopsis hyperopta and their divaricatic acid and zeorin constituents has been screened in vitro against the following species of  microorganisms: Bacillus mycoides, Bacillus subtilis,Staphylococcus aureus, Enterobacter...

Author(s): Kosanić Marijana, Ranković Branislav and Sukdolak Slobodan

May 2010

Isolation of Escherichia coli O157:H7 from manure fertilized farms and raw vegetables grown on it, in Tabriz city in Iran

  Concern for pathogens in vegetables has risen because of increasing number of outbreaks of foodborne illnesses caused by consumption of fresh and minimally processed vegetables. Using bovine feces as fertilizer, which is the common organic fertilizer on farmlands in Iran, can pose risk of spreading and transmission of enteric pathogens such as Escherichia coli O157:H7 both by direct contact and...

Author(s): Jalil Khandaghi, Vadood Razavilar and Abolfazl Barzgari

May 2010

Function analysis of the predicted protein-tyrosine phosphatases gene (DR2161) in Deinococcus radiodurans

  Protein phosphorylation on tyrosine has been demonstrated to occur in a wide array of bacterial species and appears to be ubiquitous among prokaryotes. InDeinococcus radiodurans, after the predicted protein-tyrosine phosphatase (PTP) gene DR2161 was deleted, the radiation resistance of the bacterium changed a little. The natural resistance-associated macrophage protein gene DR1709 was the possible target of...

Author(s): Shu Haiyan and Tian Baoming