African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2140

Full Length Research Paper

Rats cholestasis instigated by 𝛼-Naphthylisothiocyanate and the impacts of green tea (Camellia sinensis) antioxidant

Wael M. Al-Amoudi
  • Wael M. Al-Amoudi
  • Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
  • Google Scholar


  •  Received: 27 December 2018
  •  Accepted: 01 February 2019
  •  Published: 22 February 2019

Abstract

𝛼-Naphthylisothiocyanate (ANIT) is known to provoke liver damage with intrahepatic cholestasis. This ANIT is accepted to be important for assessing the effect of medicine instigated cholestasis. This assessment investigated the effect of green tea (Camellia sinensis) in contrast to that of Vitamin C (VC) against the hepatotoxicity of ANIT. Rats were orally treated with green tea portion (GT50, 75, 100 mg/kg) and CV (250 mg/kg) following 12 h of ANIT mixture (75 mg/kg). Rats were killed 24 h after treatment. Rats treated with ANIT demonstrated hepatocyte damage and cholestasis appeared as changes in serum biomarker levels, among others; increase in entire cholesterol, triglycerides, phospholipids, and lipid peroxide, and furthermore, an extension in hepatic lipid peroxide, a decline in glutathione and myeloperoxidase activity and a decrease in hepatic superoxide dismutase. The utilization of GT (75 mg/kg) to rats treated with ANIT blocked hepatic cell mischief and cholestasis and alleviated these serum and hepatic biochemical changes, while the use of GT (50 or 100 mg/kg) was less impressive. Both VC and ANIT-treated rats kept up a vital separation from liver cell hurt, yet not cholestasis, and reduced serum lipid peroxide, hepatic lipid peroxide, and myeloperoxidase action. These results demonstrate that the GT guarantees ANIT-affected liver harm (Cholestasis in the rat) more satisfactorily than VC.

Key words: Antioxidant, 𝛼-Naphthylisothiocyanate, cholestasis, green tea, Vitamin C.