African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2194

Full Length Research Paper

Antioxidant properties of antiepileptic drugs levetiracetam and clonazepam in mice brain after in vitro-induced oxidative stress

Aline de Albuquerque Oliveira
  • Aline de Albuquerque Oliveira
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Maria Isabel Linhares
  • Maria Isabel Linhares
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Adriano José Maia Chaves Filho
  • Adriano José Maia Chaves Filho
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Emiliano Ricardo Vasconcelos Rios
  • Emiliano Ricardo Vasconcelos Rios
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Camila Nayane de Carvalho Lima
  • Camila Nayane de Carvalho Lima
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Edith Teles Venancio
  • Edith Teles Venancio
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Alana Gomes de Souza
  • Alana Gomes de Souza
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Klistenes Alves de Lima
  • Klistenes Alves de Lima
  • Pharmacy Department, Faculty of Dentistry, Nursing and Pharmacy, Federal University of Ceará, Brazil
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Francisca Cléa Florenço de Sousa
  • Francisca Cléa Florenço de Sousa
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Danielle Macedo Gaspar
  • Danielle Macedo Gaspar
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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Marta Maria de França Fonteles*
  • Marta Maria de França Fonteles*
  • Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil.
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  •  Received: 16 May 2015
  •  Accepted: 18 February 2016
  •  Published: 15 April 2016

Abstract

This study aimed to investigate the in vitro antioxidant activity of the novel anticonvulsant levetiracetam and benzodiazepine clonazepam. To do this, the mice brain homogenates were incubated with levetiracetam (50, 100 or 200 g/ml) or clonazepam (50, 100 or 200 g/ml), and then, submitted to heating at 37°C for 1 h. Ascorbic acid (vitamin C, 200 g/ml) was used as reference antioxidant drug. The markers of oxidative stress, such as lipid peroxidation, nitrite-nitrate content, catalase activity, and reduced glutathione (GSH) levels, were measured in brain homogenates. The group submitted to the heating-induced oxidative stress showed an increase in lipid peroxidation, nitrite-nitrate content, and catalase activity. Previous incubation with levetiracetam and clonazepam, mainly at lower doses (50 and 100 g/ml), and similarly to vitamin C, prevented these pro-oxidative changes, reducing the lipid peroxidation, nitrite-nitrate contents and catalase activity, and increasing GSH levels. These findings demonstrate antioxidant properties of levetiracetam and clonazepam, and help to elucidate the role of protection against oxidative stress in the neuroprotective mechanism of antiepileptic drugs.

Key words: Levetiracetam, clonazepam, oxidative stress, vitamin C, antiepileptic drugs.