African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2191

Full Length Research Paper

Feasibility study of manufacturing coated tablet lipase from Yarrowia lipolytica

Wazé A. Mireille Alloue-Boraud*
  • Wazé A. Mireille Alloue-Boraud*
  • 1. Walloon Centre of Industrial Biology (CWBI) Unit of Bio-Industries University of Liège Gembloux Agrobio-tech, Passage des Déportés, 2-B-5030 Gembloux, Belgium. 2. Department of Food Science and Technology, Laboratory of Biotechnology and Food Microbiology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.
  • Google Scholar
Karim Amighi
  • Karim Amighi
  • Laboratory of Biopharmaceutics and galenic Pharmaceutical, Free University of Brussels, Campus de la plaine, boulevard du triomphe CP207, 1050 Bruxelles, Belgium.
  • Google Scholar
Florent K. N’Guessan
  • Florent K. N’Guessan
  • Department of Food Science and Technology, Laboratory of Biotechnology and Food Microbiology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.
  • Google Scholar
Rose Koffi-Nevry
  • Rose Koffi-Nevry
  • Department of Food Science and Technology, Laboratory of Biotechnology and Food Microbiology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.
  • Google Scholar
Jacqueline Destain
  • Jacqueline Destain
  • Walloon Centre of Industrial Biology (CWBI) Unit of Bio-Industries University of Liège Gembloux Agrobio-tech, Passage des Déportés, 2-B-5030 Gembloux, Belgium.
  • Google Scholar


  •  Accepted: 11 February 2014
  •  Published: 22 February 2014

Abstract

Many studies have provided evidence to use microbial enzyme lipase such as Yarrowia lipolytica lipase as substitute of pancreatic lipase. However Y. lipolytica lipase lowest resistance at acid pH (1 to 2) limits its application in pharmaceutical industries. In order to improve Y. lipolytica lipase resistance to low acid pH, our study was devoted more particularly to coating lipase tablets by Eudragit L30D-55. Lipase tablets were evaluated for weight uniformity, enzyme content, friability, hardness, according to European pharmacopeia. In vitro release was studied using pH 1.2 acidic buffer and pH 6.8 phosphate buffer. Study revealed that the prepared tablets were able to sustain enzyme release into the intestine. Y. lipolytica lipase tablet was compared to Creon 150 mg (commercial drug). After 3 h incubation, coated tablets did not release lipase at pH 1.2 but was disintegrated totality at pH 6.8 same as Creon 150 mg at 17 min. After 8, 12 and 24 months storage at 25 and 37°C, enzyme tablets retain their enzymatic activity and proprieties.

Key words: Tablet, creon, Eudragit L30D-55, in vitro release.