African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2108

Full Length Research Paper

Evaluation of antioxidant capacity of the aqueous extract of Cynara scolymus L. (Asteraceae) in vitro and in Saccharomyces cerevisiae

George Laylson da Silva Oliveira
  • George Laylson da Silva Oliveira
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
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Francisco Rodrigo de Asevedo Mendes de Oliveira
  • Francisco Rodrigo de Asevedo Mendes de Oliveira
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
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Marcus Vinícius Oliveira Barros de Alencar
  • Marcus Vinícius Oliveira Barros de Alencar
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
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Antonio Luiz Gomes Junior
  • Antonio Luiz Gomes Junior
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
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Alexandre Araujo de Souza
  • Alexandre Araujo de Souza
  • Federal University of Piauí, CCN, Chemistry Department, Ininga, Teresina, PI, 64048-901, Brazil.
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Ana Amélia de Carvalho Melo Cavalcante
  • Ana Amélia de Carvalho Melo Cavalcante
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
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Rivelilson Mendes de Freitas
  • Rivelilson Mendes de Freitas
  • Post-Graduation Program in Pharmaceutics Science, Federal University of Piaui, Teresina, PI, 64048-901, Brazil.
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  •  Accepted: 03 February 2014
  •  Published: 08 February 2014

Abstract

The present study evaluated the antioxidant capacity of the aqueous extract of the leaves of Cynara scolymus on Saccharomyces cerevisiae strains, proficient and deficient in antioxidant defenses, and by in vitro methods with 1,1-diphenyl-2-picrylhydrazyl (DPPH•), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), inhibition of hydrogen peroxide, lipid peroxidation, formation of nitric oxide, and removal of the hydroxyl radical. A simultaneous quantitative analysis to total phenolics, flavonoids and hydrolysable tannin was also performed, and according to the results obtained, phenolic compounds (661.15 ± 20.11 mg GAE/g of E), flavonoids (123.96 ± 11.47 mg R/g of E) and hydrolysable tannin (14.25 ± 3.18 mg GAE/g of E) were detected in the aqueous extract of C. scolymus. The aqueous extract at study showed high antioxidant capacity in all antioxidant methods in vitro and exhibited significant antioxidant capacity to protect strains of S. cerevisiae from oxidative damage induced by hydrogen peroxide. The analysis of the correlation made between the content of phenolic compounds and the different antioxidants in vitro methods, indicated that these compounds are mainly responsible for the antioxidant capacity of the aqueous extract of C. scolymus. Therefore, this study suggests that the aqueous extract of leaves of C. scolymus is a great natural source of compounds with antioxidant capacity.

Key words: Antioxidant capacity, Cynara scolymus, Saccharomyces cerevisiae, phenolic compounds.