African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2162

Full Length Research Paper

Evaluation of the antioxidant activity, antimicrobial effect and acute toxicity from leaves of Allophylus edulis (A. St.-Hil., A. Juss. Cambess &.) Hieron. ex Niederl

Cleide Adriane Signor Tirloni
  • Cleide Adriane Signor Tirloni
  • Faculdade de Ciencias da Saude - FCS, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil.
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Luis Fernando Benites Macorini
  • Luis Fernando Benites Macorini
  • Faculdade de Ciencias da Saude - FCS, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil.
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Uilson Pereira dos Santos
  • Uilson Pereira dos Santos
  • Faculdade de Ciencias da Saude - FCS, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil.
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Paola dos Santos da Rocha
  • Paola dos Santos da Rocha
  • Faculdade de Ciencias Biologicas e Ambientais - FCBA, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil
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Salete Verônica Barros
  • Salete Verônica Barros
  • Faculdade de Ciencias Biologicas e Ambientais - FCBA, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil
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Adriana Mary Mestriner Felipe de Mello
  • Adriana Mary Mestriner Felipe de Mello
  • Faculdade de Ciencias Biologicas e da Saude, Centro Universitario da Grande Dourados, Jardim Universitario, CEP 79824-900, Dourados, Mato Grosso do Sul, Brasil.
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Maria do Carmo Vieira
  • Maria do Carmo Vieira
  • Faculdade de Agronomia - FCA, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil.
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Kely de Picoli Souza
  • Kely de Picoli Souza
  • 1. Faculdade de Ciencias da Saude - FCS, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil., 2. Faculdade de Ciencias Biologicas e Ambientais - FCBA, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil.
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Edson Lucas dos Santos*
  • Edson Lucas dos Santos*
  • 1. Faculdade de Ciencias da Saude - FCS, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil., 2. Faculdade de Ciencias Biologicas e Ambientais - FCBA, Universidade Federal da Grande Dourados, CEP 79804-970, Dourados, Mato Grosso do Sul, Brasil.
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  •  Received: 09 January 2015
  •  Accepted: 16 March 2015
  •  Published: 22 March 2015

Abstract

Allophylus edulis is a Brazilian plant  commonly used in the mid-west region of Brazil for treatment of disorders  related to oxidative stress such as diabetes, inflammation, hypertension and digestive diseases. The aims of the present study were to quantify flavonoids and phenolic compounds, evaluate the antioxidant activity, antimicrobial effect and acute toxicity of leaves of A. edulis. Ethanolic (EEAE) and aqueous (AEAE) extracts of A. edulis were prepared. The antioxidant activity was determined by 2.2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging,  lipid peroxidation and oxidative hemolysis induced by 2.2'-azobis (2-amidinopropane). The antimicrobial assays of diffusion on solid media and broth microdilution were performed against Staphylococcus aureus, Escherichia coli and Candida albicans. The acute toxicity was assessed in Wistar rats treated with doses of 2 and 5 g/kg of body weight. The EEAE presented higher concentrations of flavonoids and phenolic compounds, and higher activity of scavenge DPPH free radicals. In addition, it was more effective against S. aureus compared to AEAE. The extracts were unsuccessful against E. coli and C. albicans. The EEAE prevented the lipid peroxidation in human erythrocytes and inhibited oxidative hemolysis in all  the concentrations assessed. During the evaluation of acute toxicity, the dose of 5 g/kg of body weight increased hepatic mass. Together, these results demonstrated that the EEAE of leaves of A. edulis is more effective than the AEAE, showing antioxidant activity and antimicrobial effect against S. aureus, as well as low toxicity.

Key words: Cocum, native Brazilian plant, lipid peroxidation, DPPH, AAPH, TBARS, malondialdehyde, Sapindaceae.