African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Full Length Research Paper

Assessment of antimalarial activity and proteomics analysis of Dioscorea membranacea Pierre.

Phunuch Muhamad
  • Phunuch Muhamad
  • Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathumtani 12121, Thailand.
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Luxana Panrit
  • Luxana Panrit
  • Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathumtani 12121, Thailand.
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Artitiya Thiengsusuk
  • Artitiya Thiengsusuk
  • Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathumtani 12121, Thailand.
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Wanna Chaijaroenkul
  • Wanna Chaijaroenkul
  • Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12121, Thailand.
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Kesara Na-Bangchang
  • Kesara Na-Bangchang
  • Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12121, Thailand.
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  •  Received: 15 June 2018
  •  Accepted: 03 July 2018
  •  Published: 22 July 2018

Abstract

Multidrug resistance Plasmodium falciparum remains a significant global health problem worldwide. New alternative antimalarial drugs are urgently needed. Dioscorea membranacea Pierre. is a Thai-medicinal plant that has been shown to exhibit a wide range of pharmacological activities. The study aimed to investigate antimalarial activity and possible protein targets of action of the crude ethanolic extract of the rhizome of this plant.  The in vitro antimalarial activity expressed as IC50 (concentration that inhibits the parasite growth by 50%) of the extract against 3D7 chloroquine-sensitive P. falciparum and K1 chloroquine-resistant P. falciparum clones were 10.1 (8.8-10.3) and 9.3 (9.17-9.63) µg/ml [median (range)], respectively. The cytotoxicity of against the human fibroblast cell OUMS-36T-1F was 96.4 (96.3-96.5) µg/ml.  The selectivity index (SI) for the 3D7 and K1 clones was 9.5 and 10.4, respectively. Preliminary investigation of the protein targets of action in 3D7 P. falciparum clone revealed 13 up-regulated protein spots and 14 down-regulated protein spots. For further development of D. membranacea Pierre. as a promising antimalarial drug candidate, identification of these proteins by mass spectrometry and investigation of their mode of antimalarial actions are encouraged.  

Key words: Malaria, proteomics, Dioscorea membranacea Pierre.

 

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