African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 688

Full Length Research Paper

Hairy roots production in Phyllanthus odontadenius Müll. Arg. by seedlings transformed with Agrobacterium rhizogenes A4RS/pHKN29

Kikakedimau Nakweti R.*
  • Kikakedimau Nakweti R.*
  • Laboratoire de Biotechnologie et Biologie moléculaire, Division Sciences de la vie, CGEA/CREN-K., Kinshasa, République Démocratique du Congo.
  • Google Scholar
Virginie Vaissayre
  • Virginie Vaissayre
  • Rhizogenes Lab, UMR-DIADE, Institut de Recherche pour le Développement (IRD), Montpellier, France.
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Diamuini Ndofunsu A.
  • Diamuini Ndofunsu A.
  • Laboratoire de Biotechnologie et Biologie moléculaire, Division Sciences de la vie, CGEA/CREN-K., Kinshasa, République Démocratique du Congo.
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Luyindula Ndiku S.
  • Luyindula Ndiku S.
  • Laboratoire de Biotechnologie et Biologie moléculaire, Division Sciences de la vie, CGEA/CREN-K., Kinshasa, République Démocratique du Congo.
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Jocelyne Bonneau
  • Jocelyne Bonneau
  • Rhizogenes Lab, UMR-DIADE, Institut de Recherche pour le Développement (IRD), Montpellier, France.
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Claudine Franche
  • Claudine Franche
  • Rhizogenes Lab, UMR-DIADE, Institut de Recherche pour le Développement (IRD), Montpellier, France.
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  •  Received: 03 March 2009
  •  Accepted: 25 December 2014
  •  Published: 28 February 2015

Abstract

Phyllanthus odontadenius Müll. Arg is one of the most important medicinal plants in the genus Phyllanthus (Phyllanthaceae). It is used in different regions in the world for the treatment of various diseases for example malaria caused by Plasmodium falciparum causing millions deaths in tropical and subtropical regions. In this work, we experimented P. odontadenius seeds germination and seedlings infected by Agrobacterium rhizogenes A4RS/pHKN9. We obtained 36% of seeds that germinated for 84.6% of seedlings transformed which showed positive gfp activities. The number of hairy roots formed in infectious sites for seedlings infected with A. rhizogenes A4RS/pHKN29 is valued at 6.3±2.71 against 0 for control seedlings. Diagrams showed that mean length for the new-formed roots were 1.92±0.55 cm and 1.59±0.49 cm for hairy root.   

 

Key words: Phyllanthus odontadenius, hairy roots, Agrobacterium rhizogenes, malaria, secondary metabolites.