International Journal of
Plant Physiology and Biochemistry

  • Abbreviation: Int. J. Plant Physiol. Biochem.
  • Language: English
  • ISSN: 2141-2162
  • DOI: 10.5897/IJPPB
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Estimation of auxins and cytokinins requirements in sugarcane soma clones for effective in vitro regeneration procedure

Sardar Khatoon Solangi
  • Sardar Khatoon Solangi
  • Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan.
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Sadaf Tabasum Qureshi
  • Sadaf Tabasum Qureshi
  • Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan.
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Mukhtiar Khatoon Solangi
  • Mukhtiar Khatoon Solangi
  • Sindh Agriculture University of Tando Jam, Pakistan.
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Nusrat Solangi
  • Nusrat Solangi
  • Sindh Agriculture University of Tando Jam, Pakistan.
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Altaf Hussain Solangi
  • Altaf Hussain Solangi
  • Sindh Agriculture University of Tando Jam, Pakistan.
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Mehnaz Qamar
  • Mehnaz Qamar
  • Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan.
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  •  Received: 25 September 2016
  •  Accepted: 06 July 2017
  •  Published: 31 March 2018

Abstract

Three sugarcane varieties were used in this experiment to optimize specific plant hormones. The present work was conducted in 2014 at Nuclear Institute of Agriculture (NIA), Tando jam. The experiment was designated with three sugarcane varieties (NIA-2012, NIA-105 and GULABI-95) obtained from Nuclear Institute of Agriculture (NIA) Tando jam. Regeneration of plantlets was compared under different concentration of auxins and cytokinin (IAA, IBA and kinetin (2.0, 3.0 mg1-1), highly significant (p<0.05) variations were observed for all parameters of regeneration and root formation. Interactive effect of variety x treatment x concentration was non-significant for number of regenerated plantlets. Auxins and cytokinins at 3.0 + 3.0 mg1-1 concentration were most optimized and effective for regenerated plantlets and number of shoots. These concentrations should be used in the future for in vitro culture of sugarcane.

Key words: In vitro, regeneration, cytokinins, sugarcane, growth regulators.