International Journal of
Science and Technology Education Research

  • Abbreviation: Int. J. Sci. Technol. Educ. Res.
  • Language: English
  • ISSN: 2141-6559
  • DOI: 10.5897/IJSTER
  • Start Year: 2010
  • Published Articles: 79

Full Length Research Paper

Laboratory report on quantification of anacardic acids from pistachio hulls

Aborah Martin
  • Aborah Martin
  • Department of Chemistry and Advanced Chemical Methodologies, School of Science and Technology, University of Camerino, Italy.
  • Google Scholar

  •  Received: 30 January 2022
  •  Accepted: 31 March 2022
  •  Published: 30 November 2022


Pistachio hull contains anacardic acids. It is a salicylic acid with a side chain either saturated or unsaturated. This work focused on the efficient extraction of anacardic acids using solvent. Six aliquots were weighed and extracted with hexane, ethyl acetate, and mixture of H2O/MeOH/HCl. Hexane was the most efficient solvent due to its ability to dissolve the anacardic acids. Only one anacardic acid standard was used to quantify all of them since the side chain does not affect the wavelength to a greater extent. HPLC-DAD was used for quantification purposes and HPLC-MS and retention time were used for qualitative analysis.


Key words: Anacardic acid, maceration, salicylic acid, pistachio hulls.