Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 157

Full Length Research Paper

Antimicrobial activity of crude extract from Millettia ferruginea leaves and barks against selected bacterial pathogens and Candida albicans

Mesay Bahiru
  • Mesay Bahiru
  • Department of Biology, College of Natural and Computational Science, Dilla University, Ethiopia.
  • Google Scholar
Geremew Tafesse
  • Geremew Tafesse
  • Department of Biology, College of Natural and Computational Science, Dilla University, Ethiopia.
  • Google Scholar
Nitin M. Chauhan
  • Nitin M. Chauhan
  • Department of Biology, College of Natural and Computational Science, Dilla University, Ethiopia.
  • Google Scholar
Ermias Assefa
  • Ermias Assefa
  • Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia.
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  •  Received: 26 February 2019
  •  Published: 31 August 2020

Abstract

The progressive increase in antimicrobial resistance was the major attribute among human pathogens that has given rise to the need to investigate other sources of therapy from various sources such as medicinal plants. The aim of this study was to assess the antibacterial activity of crude extract of leaves and bark of Millettia ferruginea against human pathogenic bacteria (Staphylococcus aureus and Shigella boydii) and Candida albicans. Maceration technique was employed for extraction of the plant by using solvents of different polarity levels. Antibacterial and antifungal activities of each crude extracts were evaluated at a concentration of 100, 200 and 300 mg/ml via agar well diffusion assay. The standard drugs of 30 μg/ml and 1 ml of DMSO were used as positive and negative control respectively. Minimum inhibitory concentration was also determined. The result of the study indicated that all the three solvent extracts of leaf of M. ferruginea did not show antibacterial activity for test pathogens. Acetone and chloroform bark extracts showed antibacterial activity at all three concentrations. Minimum inhibitory concentration (MIC) for acetone bark extracts were 12.5 and 25 mg/ml for S. aureus and S. boydii respectively and also for chloroform extracts 25 and 50 mg/ml for S. aureus and S. boydii respectively. Ethanol bark extracts showed antibacterial activity only for S. aureus at higher concentrations (200 and 300 mg/ml) with minimum inhibitory concentration (MIC) of 200 mg/ml. Acetone extract at 300 mg/ml was insignificantly different from the antibiotic Gentamicine (P = 0.12). The present results concluded that out of three leaf extracts and three bark extracts, only chloroform and acetone extracts of bark have antibacterial activity against either S. aureus or S. boydii, while none of the prepared extracts had antifungal action.

 

Key words: Millettia ferruginea, MIC, in vitro natural antimicrobial assay, Staphylococcus aureus, Shigella boydii.