Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3753

Full Length Research Paper

Chemical investigation, toxic potential and acetylcholinesterase inhibitory effect of Parkia platycephala leaf and seed extracts

Rachel de Moura Nunes Fernandes
  • Rachel de Moura Nunes Fernandes
  • Postgraduate Program in Biodiversity and Biotechnology - BIONORTE, Federal University of Tocantins, Palmas, TO, Brazil.
  • Google Scholar
Maria Angelica Melo Rodrigues
  • Maria Angelica Melo Rodrigues
  • Environmental Engineering Course, Federal University of Tocantins, Palmas, TO, Brazil
  • Google Scholar
Juliane Farinelli Panontin
  • Juliane Farinelli Panontin
  • Postgraduate Program in Environmental Sciences - CIAMB, Federal University of Tocantins, Palmas, TO, Brazil
  • Google Scholar
Daniela Ribeiro Alves
  • Daniela Ribeiro Alves
  • Laboratory of Chemistry of Natural Products, Center for Science and Technology, State University of Ceará, Fortaleza, CE, Brazil
  • Google Scholar
Selene Maia Morais
  • Selene Maia Morais
  • Laboratory of Chemistry of Natural Products, Center for Science and Technology, State University of Ceará, Fortaleza, CE, Brazil
  • Google Scholar
Ilsamar Mendes Soares
  • Ilsamar Mendes Soares
  • Degree Course in Biological Sciences, Federal Institute of Tocantins, Araguatins, TO, Brazil
  • Google Scholar
Elisandra Scapin
  • Elisandra Scapin
  • Postgraduate Program in Biodiversity and Biotechnology - BIONORTE, Federal University of Tocantins, Palmas, TO, Brazil. 2 Environmental Engineering Course, Federal University of Tocantins, Palmas, TO, Brazil 3 Postgraduate Program in Environmental Sciences - CIAMB, Federal University of Tocantins, Palmas, TO, Brazil
  • Google Scholar


  •  Received: 16 June 2021
  •  Accepted: 10 September 2021
  •  Published: 30 September 2021

Abstract

The present work evaluated the inhibitor potential of acetylcholinesterase, the preliminary toxicity and determination of active components of different leaf and seed extracts of Parkia platycephala. All extracts were obtained through hot extraction in a closed system (Soxhlet). To obtain the leaf and seed hexanic, methanolic, and ethanolic extracts (LHE, LME, LEE, and SHE, SME, and SEE), sequential extraction was performed on the same plant sample using hexane, methanol, and hydroethanol solution (70%). Preliminary Phytochemical analysis and the characterization by gas chromatography coupled to the mass spectrometer (GC-MS) was performed. The content of phenols total flavonoids and the antioxidant potential was then quantified.The preliminary toxicity against Artemia salina was also evaluated and the potential for acetylcholinesterase inhibition was determined. The presence of tannins, flavonoids, saponins, phytosterols/triterpenoids and alkaloids were detected in phytochemical analysis. The leaf extracts showed antioxidant potential, LEM (IC50 = 30.19 ± 0.75 μg/ml) and LEE (IC50 = 40.62 ± 0.65 μg/ml). The analysis by GC-MS indicated a diversity of volatile compounds, evidencing urs-12-ene (triterpenoid) and 1,2,3-benzenetriol (phenol) in the leaf extracts, and linoelaidic acid (fatty acid), (Z)-9-octadecenamide, tricycle [20.8.0.0 (7.16)] triacontane,1(22),7(16)-diepoxy-, (Z)-7-hexadecenal (fatty aldehyde) in the seed extracts. The preliminary toxicity analysis demonstrated that the use of P. platyceplaha leave and seeds for medicinal purposes is relatively safe. All the extracts inhibited acetylcholinesterase, compared to the physostigmine control, with IC50 values in the range of 9.85 to 15.68 mg/ml. Thus, these data support the use of P. platycephala as a potential therapy for Alzheimer's disease.

Key words: Phytochemistry, antioxidant activity, toxicity, fava-de-bolota, Alzheimer.