Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3823

Full Length Research Paper

Effect of fermentation temperature and time on the chemical composition of bush tea (Athrixia phylicoides DC.)

L. N. Hlahla*, F. N. Mudau and I. K. Mariga
Department of Soil Science, Plant Production and Remote Sensing, University of Limpopo, Private Bag X1106, Sovenga, 0727, Republic of South Africa.
Email: [email protected]

  •  Accepted: 01 April 2010
  •  Published: 04 May 2010


A study was conducted to determine the effect of fermentation temperature and time on chemical composition of bush tea (Athrixia phylicoides DC.). Bush tea was fermented in incubators at different temperatures and for different times for quality improvement. Treatments for fermentation temperature consisted of control (24°C) room temperature, 30, 34, 38 and 42°C where the tea leaves were fermented for 30 min. Treatments for fermentation time consisted ofcontrol (0), 60, 90 and 120 min at an incubator temperature of 22 - 26°C. Acompletely randomized design (CRD) was used with three replicates for both evaluations. The chemical analysis (polyphenols, tannins and antioxidants) were done using Waterman and Mole (1994) method. The results of this study demonstrated that fermentation temperature significantly increases polyphenols at 30, 34 and 38°C whereas tannin content showed a great reduction at 38 and 42°C. Increasing fermentation time achieved a significant increase in both polyphenols (60 and 90 min) and tannin contents (90 and 120 min). However, changes in either fermentation temperature or time did not give any significant influence on antioxidant content of bush tea.


Key words: Polyphenols, tannins, antioxidants, flavour.