Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Antioxidant and antimicrobial activity of different extracts from leaves and roots of Jovibarba heuffelii (Schott.) A. Löve and D. Löve

Danijela Dimitrijević1*, Milan Stanković2, Zorica Stojanović-Radić1, Vladimir RanÄ‘elović1 and Dmitar Lakušić3
1Faculty of Sciences and Mathematics, University of Niš, 18000 Niš, Serbia. 2Faculty of Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. 3Faculty of Biology, Institute of Botany and Botanical Garden, Jevremovac”, University of Belgrade, 11000 Belgrade, Serbia.
Email: [email protected]

  •  Accepted: 13 April 2012
  •  Published: 29 August 2012


The present study investigated antimicrobial and antioxidant activity, total phenolic content and flavonoid concentration of methanolic, acetone and ethyl acetate extracts from fresh leaves and roots of Jovibarba heuffelii (Schott.) A. Löve and D. Löve. The extracts were prepared and used to evaluate the antimicrobial activity against a panel of gram-positive and gram-negative bacteria as well as against fungi. Antimicrobial activity was evaluated using broth microdilution method. J. heuffelii extract showed moderate antibacterial activity, while its antifungal activity was significantly higher. The concentration of total phenolic compounds of the extracts from roots ranged from 47.98 to 31.33 mg of GAE/g. The concentrations of flavonoids in the tested plant extracts ranged from 5.26 to 16.44 mg of RuE/g. The high contents of total phenolic compounds and flavonoids indicated that these compounds contribute to the antimicrobial activity. Antioxidant activity of plant extracts was examined in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent and compared with the reference substances values (3-tert-butyl-4-hydroxyanisole (BHA), rutin, chlorogenic acid). The results pointed to high antioxidant activity of phenolic compounds in J. heuffeliiextracts.


Key words: Jovibarba heuffelii, antioxidant, phenolic compounds, flavonoids, antimicrobial activity.