Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Characterization of alkaloid constitution and evaluation of antimicrobial activity of Solanum nigrum using gas chromatography mass spectrometry (GC-MS)

Huda Jasim
  • Huda Jasim
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Ameera Omran Hussein
  • Ameera Omran Hussein
  • Babylon University, Centre of Environmental Research, Iraq.
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Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Muhanned Abdulhasan Kareem
  • Muhanned Abdulhasan Kareem
  • Babylon University, Centre of Environmental Research, Iraq.
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  •  Received: 29 January 2014
  •  Accepted: 20 March 2015
  •  Published: 30 April 2015

Abstract

In this study, the alkaloid compounds of Solanum nigrum have been evaluated. The chemical compositions of the leaf methanol extract of S. nigrum were investigated using gas chromatography-mass specroscopy (GC-MS). GC-MS analysis of S. nigrum alkaloid leaf methanol extract revealed the existence of the cyclopentasiloxane-decamethyl, L-proline, ethylester, 2-ethyl-1-butanol, methyl ether, cyclopentasiloxane-ocamethyl, betanedioic acid, hydroxyl, diethyl, ester, 1.1.3.3.5.5.7.7-octamethy-l-7-(2methyl-propoxy) tetrasiloxane-1, dodecanoic acid, 3-hydroxy-, ethyl ester, cyclopentasiloxane-ocamethyl, dodecanedoic acid, bistert-butyldimethylsilyl ester, 2-pyrrolidinecarboxylic acid-5-oxo, ethyl ester, 1-dodecanamine, N.N-dimethyl, cyclooctasiloxane, hexadecamethyl, 5-keto-2, 2-dimethylheptanoic acid, ethyl ester, cyclodecasiloxane, eicosamethyl, 9.12.15-octadecatrienoic acid, octadecanoic acid, octadecenal, 9-octadecenamide, octadecane, 3-ethyl-5-(ethylbutyl), N-acetyl-L-tryptophan ethyl ester, ethyl iso-allocholate, phthalic acid, di(2-propylpentyl)ester and 17-(1.5-Dimethylhexyl)-10. 13-dimethyl-2.3.4.7.8.9.10.11. 12.13.14.15.16.17-tetradecahydro-1H. Alkaloids extract from leaf of S. nigrum were assayed for in vitro antibacterial activity against Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aerogenosa and Klebsiella pneumonia using the diffusion method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters of inhibition zones ranged from 0.8 to 2.01 mm for all treatments.

Key words: Alkaloids, antibacterial activity, gas chromatography-mass specroscopy (GC-MS) analysis, Solanum nigrum.