Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 224

Full Length Research Paper

Characterization of alkaloid constitution and evaluation of antimicrobial activity of Solanum nigrum using gas chromatography mass spectrometry (GC-MS)

Huda Jasim
  • Huda Jasim
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
  • Google Scholar
Ameera Omran Hussein
  • Ameera Omran Hussein
  • Babylon University, Centre of Environmental Research, Iraq.
  • Google Scholar
Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
  • Google Scholar
Muhanned Abdulhasan Kareem
  • Muhanned Abdulhasan Kareem
  • Babylon University, Centre of Environmental Research, Iraq.
  • Google Scholar

  •  Received: 29 January 2014
  •  Accepted: 20 March 2015
  •  Published: 30 April 2015


Ajitha M, Rajanarayana K 2001. Role of oxygen free radicals in human disease. Indian Drugs.38:545-554.
Alves TM, Silva AF, Brandão M, Grandi TS, Smânia E, Smânia Júnior A, Zani C (2000). Biological Screening of Brazilian Medicinal Plants. Memórias do Instituto. Oswaldo Cruz 95:(3)367-373.
Cos P, Vlietinck AJ, Berghe DV, Maes L (2006). Anti-infective potential of natural products: How to develop a stronger in vitro proof-of-concept. J. Ethnopharmacol. 106(3) 290-302.
Evans WC (2002). Trease and Evans Pharmacognosy, 15th edition. WB Saunders Company Ltd, London. Pp. 137-139,230-240.
Haraguchi H, Kataoka S, Okamoto S, Hanafi M, Shibata K (1999). Antimicrobial triterpenes from Ilex integra and the mechanism of antifungal action. Phytother. Residence 13:151-156.
Iwu MW, Duncan AR, Okunji CO (1999). New antimicrobials of plant origin. In: Janick J (ed.), Perspectives on New Crops and New Uses. Alexandria, VA: ASHS Press. pp. 457-462.
John Britto S, Senthilkumar S (2005). Antibacterial activity of Solanum incanum L. leaf extracts. Asian J. Microbiol. Biotech. Environ. Sci. 3:65-66.
Koduru S, Grierson DS, Afolayan AJ (2006). Antimicrobial Activity of Solanum aculeastrum. Pharm. Biol. 44:266-283.
Krishnaraju AV, Rao TV, Sundararaju D (2005). Assessment of bioactivity of Indian medicinal plants using Brine shrimp (Artemia salina) lethality assay. Int. J. Appl. Sci. Eng. 2:125-134.
Kumar VP, Shashidhara S, Kumar MM, Sridhara BY (2001). Cytoprotective role of Datura Stramonium against gentamicin- induced kidney cell (vero cells) damage in vitro. Fitoterapia 72:481-486.
Misawa M (1994). Plant tissue culture. An alternative for production of useful metabolities. FAO Agriculture Services Bulletin, Rome. No. 108
Ncube NS, Afolayan AJ Okoh AI (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afr. J. Biotechnol. 7(12):1797-1806.
Palombo EA, Semple SJ (2001). Antibacterial activity of traditional Australian medicinal plants. J. Ethnopharmacol. 77(2-3):151-15.
Prescott LM, Harley JP, Klein DN (1999). Microbiology 4th ed. Bistin: The McGraw-Hill Companies Inc. pp. 685.
Purohit SS, Vyas SP (2004). Medicinal plants cultivation a scientific approach including processing and financial guidelines. 1st edition. Publishers Agrobios, Jodhpur, India. pp. 1-3.
Santh RT (2006). Antibacterial activity of Adhatoda vasica leaf extract. Asian J. Microbiol. Biotech. Environ. Sci. 8(2):287-289.
Sashikumar JM, Remya M, Janardhanan K (2003). Antimicrobial activity of ethno medicinal plants of Nilgiri biosphere reserve and Western Ghats. Asian J. Microbiol. Biotechnol. Environ. Sci. 5:183-185.
Sazada S, Arti V, Ayaz AR, Fraha J, Mukesh K (2009). Preliminary phytochemical analysis of some important medicinal and aromatic plants. 3(5-6):188-195.
Singh SK, Saroj K, Tirupathi UJ, Singh AK, Singh RH (1998). An antimicrobial principle from Speranhtus indicus. Int. J. Crude Drug 26:235-239.
Stainer RY, Ingraham JL, Wheelis ML (1986). General Microbiology, 5th ed. London: The MacMillan Press Ltd.
Venkatesan M, Vishwanathan MB, Ramesh N, Lakshmanaperumalsamy P (2005). Antibacterial potential from Indian Suregada angustifolia. J. Ethnopharmacol. 99(3):349-52.
Stanojević D, Čomić L, Stefanović O (2010). In vitro synergy between Salvia officinalis L. and some preservatives. Cent. Eur. J. Biol. 5 (4) 491-495.
Uzun E, Sariyar G, Adsersen A, Karakoc B, Otük G, Oktayoglu E, Pirildar S (2004). Traditional medicine in Sakarya Province (Turkey) and antimicrobial activities of selected species. J. Ethnopharmacol. 95(2-3):96-287.
Walton J, Brown DE (1999). Chemical from plants, perspectines on plant secondary products. Imperial College Press London UK. 2-5
World Health Organization (2002). WHO Traditional medicine strategy 2002-2005, World Health Organization.