Full Length Research Paper
ABSTRACT
Medicinal plant extracts are known to possess breast cancer antidote. The present investigation is focused on anticancer efficacy of various parts of Annona squamosa. The organic (ethanol) extracts from various parts of Annona squamosa were prepared using soxhlet apparatus and tested for in vitro anticancer efficacy on Breast cancer cell line MCF-7 by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results obtained from MTT assay showed that the inhibitory concentration values of bark, peel and seed were found to be approximately 20, 30 and 10 μg/ml, respectively The ethanolic seed extract had high anticancer activity with IC50 value of 10 ug/ml, reveals that A. squamosa inhibits the proliferation of MCF-7 by inducing apoptosis. The plant investigated has anti-cancer activity; hence further studies should be carried out for the isolation of the lead molecules from the parts of the plant to treat the breast cancer.
Key words: Annona squamosa, MCF-7 cell line, 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, anti-cancer activity.
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONCLUSION
CONFLICT OF INTERESTS
ACKNOWLEDGEMENT
REFERENCES
Avni GD, Ghulam NQ, Ramesh KG, Mahmoud El-Tamer, Jaswant Singh, Ajit KS, Yashbir SB, Subhash CT, Hari KB (2008). Medicinal Plants and Cancer Chemoprevention. Curr. Drug Metab. 9(7):581-591. |
|
Biba VS, Amily A, Sangeetha S, Remani P (2014). Anticancer, Antioxidant and Antimicrobial activity of Annonaceae family. World J. Pharm. Pharmaceutical Sci. 3(6):1595-1604. |
|
Chen Y, Chen JW, Li X (2011). Cytotoxic bistetrahydrofuran annonaceous acetogenins from the seeds of Annona squamosa. J. Natural Products 74:2477-2481. |
|
Craig Hopp D, Feras QA, Zhe-Ming Gu, McLaughlin JL (1998). Mono-THF ring annonaceous acetogenins from Annona squamosa. Int. J. Phytochem. 47(5):803-809. |
|
Edmondson JM, Linda SA, Andrew OM (1998). A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. J Tissue Cult. Methods 11:15-17. |
|
Feras QA, Xiao-Xi Liu, Jerry LM (1998). Annonaceous Acetogenins: Recent Progress. J. Natural Products 62:504-540. |
|
Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM (2010). Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int. J. Cancer. 127(12):2893-2917. |
|
Guangdong Food and Drug Administration (2004). Guangdong Chinese Materia Medica Standards. Guangdong Science and Technology Press,Guangdong. 1:194. |
|
Jerry LM (2008). Paw Paw and Cancer: Annonaceous Acetogenins from Discovery to Commercial Products. J. Natural Products 71(7):1311-1321. |
|
Kamaraj C, Bagavan A, Elango G, Zahir AA, Rajakumar G, Marimuthu S, Santhoshkumar T, Rahuman AA (2011a). Larvicidal activity of medicinal plant extracts against Anopheles subpictus & Culex tritaeniorhynchus. Indian J. Med. Res. 134:101-106. |
|
Kamaraj C, Rahuman AA, Elango G, Bagavan A, Zahir AA (2011b). Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, Haemonchus contortus. Parasitol. Res. 109:37-45. |
|
Liaw CC, Wu TY, Chang FR, Wu YC (2010). Historic perspectives on Annonaceous acetogenins from the chemical bench to preclinical trials. Planta Medica. 76:1390-1404. |
|
Lima LARL, Piment LPS, Boaventura MAD (2010). Acetogenins from Annona cornifolia and their antioxidant capacity. Food Chem. 122:1129-1138. |
|
Martino-Roaro Gerardo Zepeda-Vallejo, Eduardo Madrigal-Bujaidar (2008). Anti carcinogenic and genotoxic effects produced by Acetogenins isolated from Annona muricata. Abstracts 180:32-246. |
|
Neha P, Dushyant B (2011). Phytochemical and Pharmacological Review on Annona squamosa Linn. Int. J. Res. Pharmaceutical Biomed. Sci. 2(4):404-1412. |
|
Rajsekhar S (2011). Pharmacognosy and pharmacology of Annona squamosa: A Review. Int. J. Pharm. Life Sci. 2(10):1183-1189. |
|
Saelee C, Thongrakard V, Tencomnao T (2011). Effects of Thai medicinal herb extracts with anti-psoriatic activity on the expression on NF-kB signaling biomarkers in HaCaT keratinocytes. Molecules 16:3908-3932. |
|
Sumitra C, Krunal N (2013). In vitro and in vivo Methods for Anticancer Activity Evaluation and Some Indian Medicinal Plants Possessing Anticancer Properties: An Overview. J. Pharmacogn. Phytochem. 2(1):14-19. |
|
Suzanne AE, Aboagye EO, Ali S, Anderson AS, Armes J, Berditchevski F, Blaydes JP, Brennan K, Brown NJ, Bryant HE, Bundred NJ, Burchell JM, Campbell AM, Carroll JS, Clarke RB, Coles CE, Cook GJ, Cox A, Curtin NJ, Dekker LV, Silva Idos S, Duffy SW, Easton DF, Eccles DM, Edwards DR, Edwards J, Evans D, Fenlon DF, Flanagan JM, Foster C, Gallagher WM, Garcia-Closas M, Gee JM, Gescher AJ, Goh V, Groves AM, Harvey AJ, Harvie M, Hennessy BT, Hiscox S, Holen I, Howell SJ, Howell A, Hubbard G, Hulbert-Williams N, Hunter MS, Jasani B, Jones LJ, Key TJ, Kirwan CC, Kong A, Kunkler IH, Langdon SP, Leach MO, Mann DJ, Marshall JF, Martin L, Martin SG, Macdougall JE, Miles DW, Miller WR, Morris JR, Moss SM, Mullan P, Natrajan R, O'Connor JP, O'Connor R, Palmieri C, Pharoah PD, Rakha EA, Reed E, Robinson SP, Sahai E, Saxton JM, Schmid P, Smalley MJ, Speirs V, Stein R, Stingl J, Streuli CH, Tutt AN, Velikova G, Walker RA, Watson CJ, Williams KJ, Young LS, Thompson AM (2013). Critical research gaps and translational priorities for the successful prevention and treatment of breast cancer. Breast Cancer Res. 15(5):R92. |
|
Yang HJ, Zhang N, Li X, Chen JW, Cai BC (2009). Structure–activity relationships of diverse annonaceous acetogenins against human tumor cells. Bioorganic Medicinal Chem. Lett. 19:2199-2202. |
Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0