Journal of
Toxicology and Environmental Health Sciences

  • Abbreviation: J. Toxicol. Environ. Health Sci.
  • Language: English
  • ISSN: 2006-9820
  • DOI: 10.5897/JTEHS
  • Start Year: 2009
  • Published Articles: 212

Full Length Research Paper

Hepatotoxic and neurotoxic effects of combined lead and di-(2-ethylhexyl) phthalate exposure: Activation of total -, Ca2+- and Na+K+- ATPases in the liver of male rats

Esther Omugha Abam
  • Esther Omugha Abam
  • Biochemistry Unit, Department of Chemical and Food Sciences, College of Natural and Applied Sciences, Bells University of Technology, Ota, Ogun State, Nigeria.
  • Google Scholar
Seun Elizabeth Kuyooro
  • Seun Elizabeth Kuyooro
  • Biochemistry Unit, Department of Chemical and Food Sciences, College of Natural and Applied Sciences, Bells University of Technology, Ota, Ogun State, Nigeria.
  • Google Scholar
Shawai Abubakar
  • Shawai Abubakar
  • Biochemistry Unit, Department of Chemical and Food Sciences, College of Natural and Applied Sciences, Bells University of Technology, Ota, Ogun State, Nigeria.
  • Google Scholar
Esther Chineyenwa Dim
  • Esther Chineyenwa Dim
  • Biochemistry Unit, Department of Chemical and Food Sciences, College of Natural and Applied Sciences, Bells University of Technology, Ota, Ogun State, Nigeria.
  • Google Scholar


  •  Received: 29 June 2020
  •  Accepted: 11 August 2020
  •  Published: 30 June 2021

References

Abam E, Okediran BS, Odukoya OO, Adamson I, Ademuyiwa, O (2008). Reversal of ionoregulatory disruptions in occupational lead exposure by vitamin C. Environmental Toxicology and Pharmacology 26(3):297-304.
Crossref

 

Ademuyiwa O, Agarwal R, Chandra R, Behari, JR (2009). Lead-induced phospholipidosis and cholesterogenesis in rat tissues. Chemico-Biological Interactions 179(2-3):314-320.
Crossref

 

Ademuyiwa O, Arowolo T, Ojo DA, Odukoya OO, Yusuf AA, Akinhanmi TF (2002). Lead levels in blood and urine of some residents of Abeokuta, Nigeria. Trace elements. Electrolytes 19(2):63-69.

 

Ademuyiwa O, Ugbaja RN, Idumebor F, Adebawo O (2005). Plasma lipid profiles and risk of cardiovascular disease in occupational lead exposure in Abeokuta, Nigeria. Lipids in Health and Disease 4(1):1-7.
Crossref

 

Ademuyiwa O, Ugbaja RN, Rotimi SO, Abam E, Okediran BS, Dosumu OA, Onunkwor BO (2007). Erythrocyte acetylcholinesterase activity as a surrogate indicator of lead-induced neurotoxicity in occupational lead exposure in Abeokuta, Nigeria. vironmental Toxicology and Pharmacology 24(2):183-188.
Crossref

 

Adeniyi FAA, Anetor JI (1999). Lead poisoning in two distant states of Nigeria an indication of the real size of the problem. African Journal of Medical and Health Sciences 28(1-2):107-112.

 

Adeniyi TT, Ajayi GO, Akinloye OA (2008). Effect of Ascorbic acid and Allium sativum on tissue lead in female Rattus navigicus. Nigerian Journal of Health and Biomedical Sciences 7(2):38-41.
Crossref

 

Afolabi OK, Fatoki J, Ugbaja RN, Adekunle AS, Adedosu OT, Ademuyiwa O (2015). Arsenic and di(2- ethylhexyl) phthalate interact to alter individual effects on some oxidative stress indices in rat. Asian Journal of Pharmacology and Toxicology 3(8):24-30.

 

Afolabi OK, Ugbaja RN, Ademuyiwa O (2016). Combined arsenic and di-(2-ethylhexyl) phthalate exposure elicits responses in brain ATPases different from hepatic and renal activities in rats. Journal of Toxicology and Environmental Health Sciences 8(2):6-14.
Crossref

 

Allouche L, Hamadouche M, Touabti A, Khennouf S (2011). Effect of Long-term Exposure to Low or Moderate Lead Concentrations on Growth, Lipid Profile and Liver Function in Albino Rats. Advances in Biological Research 5(6):339-347.

 

Basaran N, Underger U (2000). Effects of lead on immune parameters in occupationally exposed workers. American Journal of Industrial Medicine 38(3):349-354.
Crossref

 

Blaustein MP, Juhaszova M, Golovina VA (1998). The cellular mechanism of action of cardiotonic steroids: a new hypothesisClinical and Experimental Hypertension 20(5-6):691-703.
Crossref

 

Blystone CR, Kissling GE, Bishop JB, Chapin RE, Wolfe GW, Foster PM (2010). Determination of the di-(2-ethylhexyl) phthalate NOAEL for reproductive development in the rat: importance of the retention of extra animals to adulthood.Toxicological Sciences 116(2):640-646.
Crossref

 

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 7(72):248-254.
Crossref

 

Capiod T (2011). Cell proliferation, calcium influx and calcium channels. Biochimie 3(12):2075-2079.
Crossref

 

Cecil KM, Brubaker CJ, Adler CM, Dietrich KN, Altaye M, Egelhoff JC, Wessel S, Elangovan I, Hornung R, Jarvis K, Lanphear BP (2008). Decreased Brain Volume in Adults with Childhood Lead Exposure. Journal Medicine 5(5):0741-0750.
Crossref

 

Dhanya CR, Indu AR, Deepadevi KV, Kurup PA (2003). Inhibition of membrane Na+ K+ ATPase of the brain, liver and RBC in rats administered Di-(2-ethylhexyl) phthalate (DEHP), a plasticizer used in polyvinyl chloride (PVC) blood storage bag. Indian Journal of Experimental Biology 41(8):814-820.

 

Dosumu O, Onunkwor B, Odukoya O, Arowolo T, Ademuyiwa O (2005). Biomarkers of lead exposure in auto-mechanics in Abeokuta, Nigeria. Trace Elem. Electrolyte 22(3):185-191.
Crossref

 

Engel SM, Miodovnik A, Canfield RL, Zhu C, Silva MJ, Calafat AM, Wolff MS (2010). Prenatal phthalate exposure is associated with childhood behavior and executive functioning. Environmental Health Perspectives 118(4):565-571.
Crossref

 

Evans DJ (1969). Membrane adenosine triphosphatase of Escherichia coli: activation by calcium ions and inhibition by monovalent cations. Journal of Bacteriology 100(2):914-922.
Crossref

 

Foster PMD, Mylchreest E, Gaido KW, Sar M (2001). Effects of phthalate esters on the developing reproductive tract of male rats. Human Reproduction Update 7(3):231-235.
Crossref

 

Fromme H, Kutcher T, Otto T, Pilz K, Muller J, Wenzer A (2012). Occurrence of phthalates and bisphenol A and F in the environment. Water Research 36(6):1429-1438.
Crossref

 

Grün F (2010). Obesogens. Current Opinion in Endocrinology, Diabetes and Obesity 17(5):453-459.
Crossref

 

Hesketh JE, Loudon JB, Reading HW, Glen IM (1978). The effect of lithium treatment on erythrocyte membrane ATPase activities and erythrocyte ion content. British Journal of Clinical Pharmacology 5(4):323-329.
Crossref

 

Hjerten S, Pan H (1983). Purification and characterization of two forms of a low-affinity Ca2+-ATPase from erythrocyte membrane. Biochimica et Biophysica Acta 728(2):281-288.
Crossref

 

Horisberger JD, Geering K (2009). Brain Na K-ATPase. Encyclopedia of Neuroscience. Science Direct. Elsevier Ltd. pp. 395-399.
Crossref

 

Horn O, Nalli S, Cooper D, Nicell J (2004). Plasticizer metabolites in the environment. Water Research 38(17):3693-3698.
Crossref

 

Ito Y, Kamijima M, Nakajima T (2019). Di(2-ethylhexyl) phthalate-induced toxicity and peroxisome proliferator-activated receptor alpha: a review. Environmental Health and Preventive Medicine 24(1):1-9.
Crossref

 

Jangid AP, Shekhawat VPS, Pareek H, Yadav D, Sharma P, John PJ (2016). Effect of lead on human blood antioxidant enzymes and glutathione. International Journal of Biochemistry Research and Review 13(1):1-9.
Crossref

 

Jia Y, Liu T, Zhou L, et al. Effects of Di-(2-ethylhexyl) Phthalate on lipid Metabolism by the JAK/STAT Pathway in Rats. International Journal of Environmental Research and Public Health 13(11):1085.
Crossref

 

Jiang T, Zi-jiang X (2008). The Na-K-ATPase and Calcium-Signaling Microdomains. Physiology 23(4):205-211.
Crossref

 

Juhaszova M, Blaustein MP (1997). Na+ pump low and high ouabain affinity alpha subunit isoforms are differently distributed in cells. Proceedings of the National Academy of Sciences of the United States of America 94(5):1800-1805.
Crossref

 

Kamrin MA (2009). Phthalate risks, phthalate regulation, and public health: a review. Journal of Toxicology and Environmental Health 12(2):157-174.
Crossref

 

Klaassen CD, Watkins JB (Eds) (2015). In Cassaret and Doull's Essentials of Toxicology. McGraw Hill Education, LANGE, New York P 277.

 

Klöting N, Hesselbarth N, Gericke M, Kunath A, Biemann R, Chakaroun R, Kosacka J, Kovacs P, Kern M, Stumvoll M, Fischer B (2015). Di-(2-ethylhexyl)-phthalate (DEHP) causes impaired adipocyte function and alters serum metabolites. PloS one 10(12):e0143190.
Crossref

 

Kosnet JM, Charles E, Becker T (1998). Basic Chemical Pharmacology. 7th ed. Appleton and Langs. Pub. pp. 321-322.

 

Krsti? D, Krinulovi? K, Vasi? V (2008). Inhibition of Na+K+ -ATPase and Mg2+-ATPase by metal ions and prevention and recovery of inhibited activities by chelators. Journal of Enzyme Inhibition and Medicinal Chemistry 20(5):469-476.
Crossref

 

Lowry OH, Lopez JA (1946). The determination of inorganic phosphate in the presence of labile phosphate esters. Journal of Biological Chemistry 162:421-428.
Crossref

 

Markovac J, Goldstein GW (1988). Lead activates protein kinase C in immature rat brain microvessels. Toxicology and Applied Pharmacology 96(1):14-23.
Crossref

 

Mata AM Sepulveda MR (2010). Plasma membrane Ca2+-ATPases in the nervous system during development and ageing. World Journal of Biological Chemistry 26(7):229-234.
Crossref

 

Matsuoka S, Nicoll DA, Hryshko LV, Levitsky DO, Weiss JN, Philipson KD (1995). Regulation of the cardiac Na+-Ca2+ exchanger by Ca2+ -mutational analysis of the Ca2+ - binding domain. Journal of General Physiology 105(3):403-420.
Crossref

 

Mayeux R, Stern Y (2012). Epidemiology of Alzheimer DiseaseCold Spring Harbor Perspectives in Medicine 2(8):a006239.
Crossref

 

Medesins Sans Frontiers (2012). Lead poisoning crisis in Zamfara state northern Nigeria paper. MSF Briefing Paper. May, 2012.

 

Miodovnik A, Engel SM, Zhu C, Ye X, Soorya LV, Silva MJ, Calafat AM, Wolff MS (2011). Endocrine disruptors and childhood social impairment. NeuroToxicology 32(2):261-267.
Crossref

 

Mitchell FE, Price SC Hinton RH, Grasso P, Bridges JW (1985). Time and dose-response study of the effects on rats of the plasticizer di(2-ethylhexyl) phthalate.Toxicology and Applied Pharmacology 81(3):371-392.
Crossref

 

Moore ED, Etter EF, Philipson KD, Carrington WA, Fogarty KE, Lifshitz LM, Fay FS (1993). Coupling of the Na+/Ca2+ exchanger, Na+/K+ pump and sarcoplasmic reticulum in smooth muscle. Nature 365(6447):657-660.
Crossref

 

Mushtaq M, Srivastava SP, Seth PK (1980). Effect of di-2-ethylhexyl phthalate (DEHP) on glycogen metabolism in rat liver. Toxicology 16(2):153-161.
Crossref

 

National Toxicology Program - Center for the evaluation of risks to human reproduction (NTP-CERHR) (2006). Monograph on the potential human reproductive and developmental effects of di(2-ethylhexyl)phthalate (DEHP).

 

Nriagu JO, Blankson ML, Ockran K (1996). Childhood lead poisoning in Africa, a growing public health problem.Science of the Total Environment 81(2):93-1000.
Crossref

 

Ohnishi T, Suzuki T, Suzuki Y, Ozawa K (1982). A comparative study of plasma membrane Mg2+- ATPase activities in normal, regenerating and malignant cells. Biochem. Biophys. Acta 684(1):67-74.
Crossref

 

Okediran BS, Abam E, Odukoya OO, Adamson I, Ademuyiwa O (2009). Membrane, intracellular, plasma and urinary sodium and potassium in occupational lead exposure: effects of vitamin C supplementation. Trace Elements and Electrolytes 26(2):49-59.
Crossref

 

Onunkwor B, Dosunmu O, Odukoya OO, Arowolo T, Ademuyiwa O (2004). Biomarkers of lead exposure in petrol station attendants and auto-mechanics in Abeokuta, Nigeria: effects of 2-week ascorbic acid supplementation. Environmental Toxicology and Pharmacology 17(3):169-176.
Crossref

 

Padmakumaran NKG, Deepadevi KV, Arun P, Manoj KV, Anitha S, Lekshmi LR, Kurup PA (1998). Toxic effect of systemic administration of low doses of the plasticiser di- (2 ethylhexyl) phthalate (DEHP) in rats. Indian Journal of Experimental Biology 36:264.

 

Pracheta M, Singh L (2009). Effect of lead nitrate (Pb(NO3)2 on plant nutrition, as well as physical and chemical parameters on Lobia (Vigna unguiculata Linn. Walp.). Journal of Plant Development Sciences 1(2):49-56.

 

Prasanthi RPJ, Reddy GH, Chetty CS, Reddy GR, (2006). Influence of calcium and zinc on lead-induced alterations in ATPases in the developing mouse brain. Journal of Pharmacology and Toxicology 1(3):245-252.
Crossref

 

Rastogi SK (2008). Renal effects of environmental and occupational lead exposure. Indian Journal of Occupational and Environmental Medicine 12(3):103-106.
Crossref

 

Rowdhwal SSS, Cheng J (2018). Toxic Effects of Di-2-ethylhexyl Phthalate: An Overview. Hindawi BioMed Research International pp. 1-10.
Crossref

 

Rusyn I, Peters J, Cunningham M (2006). Modes of action and species-specific effects of di-(2-ethylhexyl) phthalate in the liver. Critical Reviews in Toxicology 36(5):459-479.
Crossref

 

Sanders T, Liu Y, Buchner V, Tchounwou PB (2009). Neurotoxic Effects and Biomarkers of Lead Exposure: A Review. Reviews on Environmental Health 24(1):15-45.
Crossref

 

Sharma S, Sharma V, Pracheta M, Sharma SH (2011a). Therapeutic potential of hydromethanolic root extract of Withania somnifera on neurological parameters in swiss albino mice subjected to lead nitrate. International Journal of Current Pharmaceutical Research 3(2):52-56.

 

Sharma V, Sharma S, Pracheta M, Sharma SH (2011b). Lead Induced hepatotoxicity in male swiss albino mice: The protective potential of the hydromethanolic extract of Withania somnifera. International Journal of Pharmaceutical Sciences Review and Research 7:116-121.

 

Song H, Lee MY, Kinsey SP, Weber DJ, Blaustein MP (2006). An N-terminal sequence targets and tethers Na+ pump alpha2 subunits to specialized plasma membrane microdomains. Journal of Biological Chemistry 281(18):12929-12940.
Crossref

 

Stahlhut RW, van Wijngaarden E, Dye TD, Cook S, Swan SH (2007). Concentrations of urinary phthalate metabolites are associated with increased waist circumference and insulin resistance in adult U.S. males. Environmental Health Perspectives 115(6):876-882.
Crossref

 

Stewart WF, Schwartz BS (2007). Effects of lead on the adult brain: a 15-year exploration. American Journal of Industrial Medicine 50(10):729-739.
Crossref

 

Tian J, Xie ZJ (2008). The Na+-K+-ATPase and calcium-signaling microdomains. Physiology 23:205-211.
Crossref

 

Wang Y, Kim J, Schmit MB, Cho TS, Fang C, Cai H, (2019). A bed nucleus of stria terminalis microcircuit regulating inflammation-associated modulation of feeding. Nature Communications 10:2769.
Crossref

 

Wójtowicz AK, Sitarz-G?ownia AM, Szcz?sna M, Szychowski KA (2019). The Action of di-(2-ethylhexyl) phthalate (DEHP) in mouse cerebral cells involves an impairment in aryl hydrocarbon receptor (AhR) signaling. Neurotoxicity Research 35(1):183-195.
Crossref

 

Xu J, Ling-jun L, Chen WU, Xiao-feng W, Wen-yu FU, Lihong X (2008). Lead induces oxidative stress, DNA damage and alteration of p53, Bax and Bcl-2 expressions in mice. Food and Chemical Toxicology 46(5):1488-1494.
Crossref

 

Ye H, Ha M, Yang M, Yue P, Xie Z, Liu C (2017). Di2-ethylhexyl phthalate disrupts thyroid hormone homeostasis through activating the Ras/Akt/TRHr pathway and inducing hepatic enzymes. Scientific Reports 7:40153; 
Crossref

 

Yücebilgiç G, Bilgin R, Tamer L, Tükel S (2003). Effects of lead on Na+K+ ATPase and Ca2+ ATPase activities and lipid peroxidation in blood of workers. International Journal of Toxicology 2(2):95-97.
Crossref

 

Zhang W, Shen XY, Zhang WW, Chen H, Xu WP, Wei W (2017). The effects of di 2-ethyl hexyl phthalate (DEHP) on cellular lipid accumulation in HepG2 cells and its potential mechanisms in the molecular level. Toxicology Mechanisms and Methods 27(4):245-252.
Crossref