Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 369

Full Length Research Paper

Occurrence and antimicrobial resistance of Salmonella spp. in broiler chicken neck skin from slaughterhouses in Zambia

Maureen Wakwamba Ziba1,3*, Benson Bowa1, Romina Romantini2, Violeta Di Marzio2, Cristina Marfoglia2, Salvatore Antoci2, Geoffrey Muuka1, Massimo Scacchia2, Mauro Mattioli3 and Francesco Pomilio2  
1Ministry of Fisheries and Livestock, Central Veterinary Research Institute, P. O. Box 33980, Lusaka, Zambia. 2Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Via Campo Boario, 64100, Teramo, Italy. 3Unit of Basic and Applied Bioscience, Faculty of Bioscience and Agro-Food Environmental Technology, University of Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy.  
Email: [email protected]

  •  Received: 08 April 2020
  •  Accepted: 26 May 2020
  •  Published: 30 June 2020

Abstract

Salmonella is an important zoonotic foodborne pathogen and poultry meat is considered as one of its major sources. This study evaluated Salmonella spp. detected in broiler chicken carcasses in Zambia. A total of 440 broiler neck skin samples were collected from 6 slaughter houses along the process line after evisceration and tested for Salmonella spp. Eleven samples (2.5%) were positive for Salmonella spp. The suspected isolates were serotyped according to White- Kauffmann-Le Minor scheme and tested for antimicrobial susceptibility using the Sensititre broth microdilution method. Eight serovars of Salmonella enterica were confirmed namely; S. Bolton (2), S. Enteritidis (1), S. Texas (1), S. Liverpool (1), S. Chomeday (1), S. Mbandaka (1), S. Vellore (1), S. Montevideo (1). Two isolates were not typed completely giving results as S. enterica subsp. enterica O:4:Z and Salmonella enterica subsp. enterica O:3,10:Y. Antimicrobial susceptibility showed a 20% multidrug resistance in which S. Vellore and S. Mbandaka were resistant to 5 antimicrobials namely Ampicillin, Ciprofloxacin, Gentamicin, Tetracycline, Trimethoprim. S. Enteritidis, S. Bolton and Salmonella enterica subsp. enterica O:3, 10:Y were resistant to the antimicrobial Colistin. 50% of the strains were susceptible to the antimicrobials tested. This study reported Salmonella spp. in broiler chickens that have not been reported before in Zambia and showed the presence of antimicrobial resistant strains.

Key words: Salmonella serovars, foodborne disease, broiler chicken, antimicrobial resistance.