Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 354

Full Length Research Paper

Aerobic bacterial isolates, incidence rate and associated risk factors of heifer and cow mastitis in and around Debre-Libanos district, Oromia, Ethiopia

Daniel Fisseha
  • Daniel Fisseha
  • Debre-Libanos District Animal and Fish Resource Development Office, Debre-Tsege, Ethiopia.
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Tesfaye Sisay Tessema
  • Tesfaye Sisay Tessema
  • Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia.
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Biruk Tesfaye Birhanu
  • Biruk Tesfaye Birhanu
  • Department of Microbiology, Immunology and Veterinary Public Health, Bishoftu, Ethiopia.
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  •  Received: 26 November 2018
  •  Accepted: 25 June 2019
  •  Published: 31 January 2020

Abstract

The investigation was led from February-November 2014 longitudinally to recognize aerobic bacterial isolates, estimate incidence rate, identify the associated risk factors and antimicrobial sensitivity patterns of heifer and cow mastitis in and around Debre-Libanos district. An aggregate of 31 Jersey and Holstein-Fresian cross heifers that were left for less than a month to calve were sampled and pursued for the initial two lactation stages after calving. Clinical heifer mastitis was distinguished by physical examination of the udder and milk while sub-clinical one was recognized by California mastitis test. The incidence rate of heifer mastitis per gland month at risk was observed to be 37.4%. The event of new contamination in heifers and cows was not altogether influenced by gland position and udder cleanliness (P>0.05), yet by the management system, lactation stage and dry cow therapy (P<0.01). From 231 isolates, Staphylococcus aureus (25.1%) was the most prevalent pathogen pursued by coagulase negative Staphylococcus and Streptococcus species (each 14.7%). Other bacterial isolates included Micrococcus species (10.38%), Klebsiela pneumoniea (9.95%), Esherishia coli (12.98%), Corynebacterium species (5.62%), Enterobacter aerogens (4.32%) and Bacillus species (4.32%). Antimicrobial sensitivity test demonstrated that tetracycline (71.7%) was observed to be increasingly successful antibiotic among the whole tried antibiotics against all bacterial isolates while the least effective antibiotic was observed to be penicillin G (24.2%). The present investigation has demonstrated that heifer and cow mastitis specifically; sub-clinical mastitis is a critical sickness affecting heifers' milk production.

Key words: heifer mastitis, incidence rate, risk factors, Ethiopia, antimicrobial sensitivity test