Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 126

JYFR Articles

Comparative study on solubilization of tri-calcium phosphate (TCP) by phosphate solubilizing fungi (PSF) isolated from Nsukka pepper plant rhizosphere and root free soil

July 2013

Phosphate solubilizing fungi (PSF) possessing the ability to solubilize insoluble phosphate was isolated from the rhizospheric soil of Nsukka pepper plant. Twelve (12) fungal strains were isolated from both root-free soil (non-rhizosphere) and rhizosphere of Nsukka pepper plant. Of the 12 fungi isolated, 6 strains were from Nsukka pepper plant rhizosphere whereas the other six were from root- free soil. PF...

Author(s): Onyia, Chiadikobi Ejikeme and Anyanwu, Chukwudi Uzoma

Aspergillus niger-specific ribonucleic acid extraction method

July 2013

Extraction of high quality RNA from Aspergillus niger mycelia, a tissue very rich in ribonucleases was achieved using a protocol involving the use of TRIZOL reagent and the ribonuclease inhibitor (RNaseOUT). RNA was successfully isolated using this protocol that involved addition of RNaseOUT at the initial homogenizing step and the final dilution/ solubilisation steps of the RNA extraction. A sharply...

Author(s): Abdullahi Balarabe Sallau, Fiona Henriquez, Andrew Jonathan Nok, Sani Ibrahim, Caroline Sommerville and Craig Roberts

Isolation and characterization of yeast strains from local food crops

June 2013

Isolation and identification of yeast from Manihot esculenta, Zea mays, Cola acuminata andSorghum bicolor was done using the spread plate technique. Morphological, cultural, physiological and molecular characterizations were carried out resulting in determination of the species. Four isolates belonging to different genera which include Pichia, Kluyveromyces,...

Author(s): Abosede Margaret Ebabhi, Adedotun Adeyinka Adekunle, Wahab Oluwanisola Okunowo and Akinniyi Adediran Osuntoki

The effects of culture parameters on the conidial germination and yields of Ophiocordyceps sinensis

June 2013

Ophiocordyceps sinensis is an insect-born fungus with various biological and pharmacological activities. Artificial cultivation of the fungus-insect complex with fruiting body is not successful for the market mainly due to the lack of optimal conditions for fungus-insect interaction. Mass production of the pathogenic conidia as fungal inocula for effective insect infection is one of the critical steps. In...

Author(s): Caiying Mei, Yi Zhang, Xiongmin Mao, Keqing Jiang, Li Cao, Xun Yan and Richou Han

Mycotoxicological studies of an Aspergillus oryzae strain

May 2013

The safety of Aspergillus oryzae used in industrial fermentations of food-grade products has long been recognized. However, production of fungal toxic secondary metabolites is strain-specific and environment-dependent. For these reasons, the present study aimed to conduct a mycotoxicological study of the strain H/6.28.1 of A. oryzae (from the collection of the Cuban Institute of Sugar Cane...

Author(s): A. Sosa, E. Kobashigawa, J. Galindo, R. Bocourt, C. H. Corassin and C. A. F. Oliveira

Brewer’s yeast (Saccharamyces cervisiae) has hypolipidemic effect in hyperlipidemic model

May 2013

The yeast has been used for food since ancient time, brewing beer, wine and other alcoholic drinks and in baking industry to expand or to raise dough. It is relatively inexpensive food stuff that contains many kinds of beneficial nutrients [vitamins, minerals, nucleic acid, glutathione, amino acids etc]. In addition brewer's yeast is commonly known as food supplement and traditional bowel movement...

Author(s): Aisha Mohamed Alfitori, Abubaker Bashir and Ahmed Fathi Bahriz

Effect of environmental conditions on the growth of Cryptosporiopsis spp. causing leaf and nut blight on cashew (Anacardium occidentale Linn.)

March 2013

A new disease (cashew leaf and nut blight) in Tanzania caused by a fungus related to the genus Cryptosporiopsis was identified in 2006. The present work investigated the effects of environmental factors on the growth of Cryptosporiopsis spp. causing blight on cashew. The mycelial growth, colony character and sporulation pattern of 10 fungal isolates, grown on seven different culture media...

Author(s): Dominic Menge,, Martha Makobe, Shamte Shomari and Andreas. V. Tiedemann

In vitro antifungal effects of Fumaria vaillantii Loisel. essential oil on Aspergillus flavus

March 2013

      In order to identify the chemical composition of essential oil of Fumaria vaillantii, the leaves with young branches of this plant which grows in a village in Kerman Province at full flowering stage in May 2012 were collected. The sample was cleaned and then dried in the shade, and essential oil hydrodistillation method was performed. The main oil content from the plant of F. vaillantii was 0.25%...

Author(s): M. Moghtader

Isolation of Aspergillus niger from three varieties of Bambara nuts for simultaneous production of phytase and tannase

February 2013

The aim of this work was to isolate strain of Aspergillus niger for simultaneous production of phytase and tannase enzymes. In this study, A. niger strains were isolated from Bambara nuts (Vigna subterranea) of three different color seed coats. Growth of colonies in plates with tannic acid and phytic acid sodium salt-containing media indicate their ability to synthesize both enzymes. The strain...

Author(s): H. V. Difo, E. Onyike, D. A. Ameh, G. C. Njoku and U. S. Ndidi

Antagonistic confrontation of Trichoderma spp against fruit rot pathogens on Sapodilla (Manilkara zapota L.)

February 2013

Antagonistic potentials of five Trichoderma species that is Trichoderma viride, Trichoderma harzianum, Trichoderma koningii, Trichoderma pseudokoningii and Trichoderma virens were tested against fruit rots pathogens of sapodilla (Manilkara zapota L.) under laboratoryconditions. Dual culture experiment of tested pathogens and Trichoderma spp revealed...

Author(s): U. N. Bhale, P. M. Wagh and J. N. Rajkonda

Yeasts in marine and estuarine environments

December 2012

Yeasts and other fungi are prevalent in marine and estuarine ecosystems where they play an important role in the food web. Marine yeasts are unique in performing fermentations under high salt concentrations. The mechanism underlying the high salt tolerance involves the ability to accumulate high concentrations of sodium without becoming intoxicated, and the exclusion of excessive sodium from the cytoplasm. Overall, the...

Author(s): Kathiresan Kandasamy, Nabeel M. Alikunhi and Manivannan Subramanian

Antifungal effects of the essential oil from Thymus vulgaris L. and comparison with synthetic thymol on Aspergillus niger

December 2012

The antifungal effects of the essential oil from Thymus vulgaris L. and comparison with synthetic thymol on Aspergillus niger growth was studied. The chemical composition of the essential oil of T. vulgaris, the aerial parts of this plant which is grown in a village in Kerman Province at full flowering stage in June 2012 were collected. The sample was...

Author(s): M. Moghtader

The surface display of phytase on yeast cell and activity assay of the displayed protein

November 2012

Phytase is a new-style enzyme used in animal feed additive. It can increase phosphorus availability, decrease environmental phosphorus pollution and improve the performance of animals. Phytase gene was cloned by reverse transcription-polymerase chain reaction (RT-PCR) using first strand cDNA as template after reverse transcripting Aspergillus niger total RNA. The phyA gene was cloned...

Author(s): Shumin Yao, Jing Huang and Jingli Tan

Some new rust fungi (Uredinales) from Fairy Meadows, Northern Areas, Pakistan

November 2012

Puccinia opizii on Carex curta is reported here as a new record for Pakistan. Furthermore,Coleosporium lycopi, Cronartium ribicola, Melampsora epitea, Peridermium thomsonii, Phragmidium rosae-moschatae, Puccinia carthami, Puccinia chrysanthemi, Puccinia circaeae, Puccinia graminis, Puccinia komarovii and Uromyces trifolii are additions to the rust flora of Fairy...


Studies on biodiversity of some mushrooms collected in Lagos State, Nigeria using biotechnological methods

October 2012

The biodiversity of mushrooms in Lagos State, Nigeria was studied using modern biotechnological method of DNA sequence analyses. Sixteen mushrooms were collected in Lagos State for 12 months. The mushrooms collected include: Agaricus campestris, Coprinus comatus, Daldinia concetrica, Ganoderma adspersum, Ganoderma applanatum, Ganoderma lucidum, Mycena haematopus, Mycena sp., Pleurotus...

Author(s): Bankole, P. O., and Adekunle, A. A.

Fungi associated with the spoilage of berry and their reaction to electromagnetic field

October 2012

Fungi responsible for the spoilage of some berry, namely pawpaw (Carica papaya), sweet orange (Citrus sinensis), banana (Musa acuminata), garden egg (Solanum melongena), lemon (Citrus limoni), and tangelo (Citrus tangelo) with respect to their control using electromagnetic field were investigated. Fungi isolated include the genera of Aspergillus, Penicillium, Rhizopus, Articulospora, Gonatobotryum, Varicosporium,...

Author(s): B. J. Akinyele and C. O. Akinkunmi

Halophilic fungi in a polyhaline estuarine habitat

May 2012

Halophilic mycobiota was isolated from Mandovi estuary and it was dominated by Aspergillusand Penicillium species. Cladosporium and Eurotium were found in lesser numbers while obligate halophiles were found only amongst the aspergilla and they were all identified asAspergillus penicillioides. Some aspergilli and all the isolates...

Author(s): Valerie Gonsalves, Shweta Nayak and Sarita Nazareth

A comparative study of ethanol production from various agro residues by using Saccharomyces cerevisiae and Candida albicans

March 2012

In this study, ethanol produced from fruits of pineapple, orange and sweet lime was investigated. Effect of different constant times [24 and 72 h, and 8 days (in submerged fermentation)]; pH (3.5 to 8.5), temperature (78°C) and autoclave pre-treatment (121°C, 20min) were also studied to improve the yield of ethanol in fruits. Yeast (Saccharomyces cerevisiaeand Candida alb cans) are used for...

Author(s): Jayant Mishra, Deepesh Kumar, Sumeru Samanta and Manoj Kumar Vishwakarma

Generation of a cDNA library reveals the presence of a Ty-1/copia transposon in Phellinus rimosus (Hymenochaetaceae, Basidiomycota)

March 2012

Lignocellulolytic fungi have significant potential as bio-convertors with a particular capacity to degrade recalcitrant natural composts, such as lignin. We generated a cDNA library of a lignocellulolytic fungus, the Phellinus rimosus (Hymenochaetaceae, Basidiomycota), and a typical species of polypore mushroom from the Brazilian semi-arid region. To the best of our knowledge, this is the first...

Author(s): Luis Cesar Maffei Sartini Paulillo, Sizenando José de Andrade Junior, Abelmon da Silva Gesteira, Marco Brotto, and Aristóteles Góes-Neto

Antimicrobial activity and physicochemical properties of oils from tropical macrofungi

January 2012

Nutriceutics potential and physicochemical properties of the oil extract from five species of macrofungi which include Ganoderma lucidium, Pleurotus tuberregium, Termytomyces robustus, Schizophyllum commune and Trametes versicolor were investigated using standard chemical and microbiological methods. The oil was extracted using Soxhlet method of extraction. Disc diffusion and agar dilution...

Author(s): David, O. M., Fagbohun, E. D., Oluyege, A. O. and Adegbuyi, A.

First qualitative survey of filamentous fungi in Dal Lake, Kashmir

January 2012

Filamentous fungi comprehend a heterogeneous group of heterotrophic microorganisms that act as saprobes or parasites or, less frequently as symbionts living in association with other organisms. Water samples obtained seasonally from April 2010 to March 2011 at sixteen different sites of Dal Lake, Kashmir were serially diluted five folds followed by spread plate technique for the isolation of filamentous fungi, spreading...

Author(s): Suhaib A. Bandh, Azra N. Kamili, Bashir A. Ganai, Samira Saleem, Bashir A. Loneand Humera Nissa

Effect of some chemicals on growth, melanogenesis, pathogenicity and metabolic activities of Rhizoctonia solani.

October 2011

Morphogenesis, cell differentiation, sclerotial formation and melanogenesis of a fungus depend on growth conditions. The present work aims to study the effect of some chemicals on the growth parameters of Rhizoctonia solani, which is the main causal organism of many diseases as damping off disease of many crops such as Phaseolus vulgaris. Different concentrations of hydrogen peroxide H2O2 and...

Author(s): Amany H. Aboellil and Neveen M. Mohammed

Biochemical characterisation of two novel laccases from Magnaporthe grisea

October 2011

Laccases are widely distributed oxido-reductases that catalyse the biological oxidation-reduction of polyphenols with a concomitant reduction of molecular oxygen to water. Genome analysis ofMagnaporthe grisea using bioinformatic approach showed the presence of multiple laccases, which encode proteins with three domains of multicopper oxidase. The transcript levels of all M. grisea laccases were...

Author(s): Kaminee Ranka and Bharat B. Chattoo

Effect of carbon, nitrogen and trace elements on growth and sporulation of the Termitomyces striatus (Beeli) Heim

September 2011

Nutritional studies namely carbon, nitrogen and trace element requirements of Termitomyces striatus have been carried out. Amongst all the carbon compounds used, the most favourable in order of effectiveness are D (+) glucose, D (+) sucrose, maltose and D (+) raffinose. The fungus showed poor growth with lactose. Sodium nitrite served as the best inorganic nitrogen source for the growth of this fungus. Ammonium acetate,...

Author(s): Simerjit Kaur

Growth and in vitro phosphate solubilising ability of Scleroderma sinnamariense: A tropical mycorrhiza fungus isolated from Gnetum africanum ectomycorrhiza root tips

September 2011

The growth and ability of Scleroderma sinnamariense isolated from Gnetum africanumectomycorrhiza root tips to solubilise calcium tetrahydrogen diorthophosphate, calcium phytate, hydroxyapatite and amorphous iron phosphate was assessed in axenic culture under a range of conditions. S. sinnamariense grew on all P sources when NH4+ was sole nitrogen source, but failed to grow on amorphous iron...

Author(s): Eneke Esoeyang Tambe Bechem

Comparative analysis of bioethanol production by different strains of immobilized marine yeast

September 2011

Yeasts are well known for bioethanol production. However, marine yeasts are less known for the activity. In the present context of increasing demand for energy and biofuel, the microbial synthesis of ethanol using cellulosic waste materials has gained recent importance. The present study deals with the identification of potential marine yeasts for ethanol production. Ten species of marine yeasts were cultured for 24,...

Author(s): P. Senthilraja, K. Kathiresan and K. Saravanakumar

Screening of indigenous yeast isolates obtained from traditional fermented foods of Western Himalayas for probiotic attributes

September 2011

Twenty three indigenous isolates of yeast (Saccharomyces cerevisiae) obtained from various traditional fermented foods and traditional inocula of Western Himalayas (Himachal Pradesh) were subjected to in vitro probiotic tests. All the isolates were found to be intrinsically tolerant to upper gastrointestinal transit and this property was isolate dependent. Reduction in viability (in terms of log CFU/ml cells) was more...

Author(s): Aditi Sourabh, Sarbjit Singh Kanwar, and Om Prakash Sharma

Alternaria jacinthicola, a new fungal species causing blight leaf disease on water hyacinth [Eichhornia crassipes (Martius) Solms-Laubach]

August 2011

IWater hyacinth (Eichhornia crassipes) causes environmental, agricultural and health problems in Mali. This is particularly severe in the District of Bamako and the irrigation systems of the “Office du Niger” area. During two years survey for fungal pathogens of water hyacinth infested areas, isolate Mlb684 was collected from diseased plant. This fungal isolate was identified as a potential mycoherbicide for...

Author(s): Karim Dagno, Julien Crovadore, François Lefort, Rachid Lahlali, LudivineLassoisand M. Haïssam Jijakli

Imaging flow cytometric analysis of Schizosaccharomyces pombe morphology

August 2011

The morphology of Schizosaccharomyces pombe can be rapidly monitored in asynchronous, G2-rich populations using imaging flow cytometry (IFC). Cell morphology was analyzed in terms of length and aspect ratio before and after exposure to several toxins. The toxins target the DNA (hydroxyurea and phleomycin) or cytoskeletal elements (thiabendazole, carbendazim and latrunculin A) and exert well-characterized...

Author(s): Radha Pyati, Lindsay L. Elvir, C. Erica Charles, Umawattee Seenath, and Tom D. Wolkow

Fungicide toxicity against the growth of lineages of the fungus Metarhizium anisopliae var. anisopliae (Mestch.) Sorokin

July 2011

In this work, the fungicidal action of three agrochemicals (Sphere®, Nativo® and Bendazol®) used in soybean for control of fungal diseases on the lineages CG-28 and CG-30 of Metarhizium anisopliae var. anisopliae was evaluated. It was found that the fungicides inhibited the vegetative growth of the lineages at the concentrations indicated for the field, thereby showing its antifungal...

Author(s): Sideney B. Onofre, Cristiane R. Kasburg, Danusa de Freitas, Silvana Damin, Andréia Vilani, Jéssica A. Queiroz and Francini Y. Kagimura

The research of infection process and biological characteristics of Rhizoctonia solani AG-1 IB on soybean

July 2011

The isolate Rhizoctonia solani AG-1 IB collected from the diseased leaves of soybean were identified by the method of internal transcribed spacer sequence analysis. In this study, we focus on the biological characteristics and infection process of AG-1IB. Morphological, nucleus, chromosome, and infection process were observed. Typical infectious structures as infection cushion and appressorium were observed...

Author(s): Aiping Zheng and Yanran Wang

Physiological variability among isolates of Phomopsis azadirachtae from Tamil Nadu

May 2011

16 isolates of Phomopsis azadirachtae, causing die-back of neem were isolated from 16 different geographical regions of Tamil Nadu. Their growth response to physical (temperature and pH) and chemical (carbon and nitrogen sources) factors were investigated. The toxicity of culture filtrate of each isolate on neem seed germination was also studied. Several isolates exhibited maximum growth at 35°C. Maximum growth...

Author(s): K. Girish, and S. Shankara Bhat,

Use of isozyme analyses and PCR based methods RAPD and RFLP for assessment of biochemical and genetic diversity of morphologically similar ectomycorrhizal Lactarius deliciosus from India

May 2011

The present study was undertaken to assess the genetic diversity and phylogenetic relationship among the morphologically similar and ecologically variant isolates of Lactariusdeliciosus using polymerase chain reaction (PCR) based techniques random amplification of polymorphic DNA (RAPD), rDNA analysis and isozyme patterns. Two major groups were defined amongst the total isolates by cluster analyses. Four bands of...

Author(s): Mukesh K. Upadhyay, A. K. Pandey and R. C. Rajak

Two new schifffnerulaceous fungi from Kerala, India

May 2011

Two new species namely, Sarcinella loranthacearum and Schiffnerula meliosmatis are collected from Peppara and Neyyar Wildlife sanctuaries and Silent Valley National park are described and illustrated in detail. Key words: Black mildews, new species, Kerala, India.

Author(s): V. B. Hosagoudar, J. Thomas and D. K. Agarwal

Gasteroid mycobiota of Rio Grande do Sul, Brazil: Boletales

April 2011

Boletales is an order of the subclass Phallomycetidae, which comprises of a wide variety of morphological types of macrofungi, including the boletes and earthballs. In this paper, the gasteroid members of the Boletales from Rio Grande do Sul State, in southern Brazil, were revised. Specimens were collected during the years 2006 to 2009, analyzed macro and microscopically and the collections are preserved...

Author(s): Vagner G. Cortez , Iuri G. Baseia and Rosa Mara B. Silveira

In vitro antimicrobial studies of Nodulisporium specie: An endophytic fungus

April 2011

Nodulisporium sp. an endophytic fungus identified by 28s ribosomal gene sequencing isolated from a medicinal plant, Nothapodytes foetida was studied for its in vitro antimicrobial activity. Dual culture studies were carried out for antifungal activity where maximum antagonistic activity was against Alternaria alternata and Colletotrichum gleosporoides. For antibacterial...

Author(s): S. Rehman, Tariq Mir, A. Kour, P. H. Qazi, P. Sultan and A. S. Shawl

Occurrence and identification of yeast species isolated from Egyptian Karish cheese

April 2011

This study aims at identifying the diversity and abundance of yeast associated with Egyptian Karish cheese, employing comparison between conventional laboratory techniques and API20 kits techniques in yeast identification. A total of one hundred samples (fifty each) of Egyptian raw and pasteurized Karish cheese milk were randomly collected from farmers and markets in Cairo and Giza Districts. The occurrence of yeast in...

Author(s): Neveen S. M. Soliman and Salwa A. Aly

Amylolytic enzymes produced by the fungus Colletotrichum gloeosporioides in rice semi-solid fermentation

March 2011

Amylases are enzymes that hydrolyze starch, releasing several products including dextrines and small polymers of glucose units. This work investigated the capacity of the endophytic fungus Colletotrichum gloeosporioides of producing the amylolytic enzymes ï¡-amylase and glucoamylase through fermentation in semi-solid medium of residues of the processing of rice, without supplementation. The ï¡-amylase...

Author(s): Sideney Becker Onofre, Paula Steilmann, Julia Bertolini, Daniele Rotta, Aline Sartori Francini, Yumi Kagimura, Sara Ângela Groff and Luciana Mazzali

Survey of Spilocaea oleagina, causal agent of olive leaf spot, in North of Iran

March 2011

Olive leaf spot or peacock spot, caused by the fungal pathogen Spilocaea oleagina, can cause reduced growth and yield in olive trees (Olea eurapaea). Investigations were carried out during 2007-2010 to measure the prevalence and severity of olive leaf spot in the northern olive growing regions of Iran. The susceptibility of ten cultivars (Amygdalifolia, Blaidy, Koronakei, Mary, Manzanillo, Mission, Rooghany,...

Author(s): S. J. Sanei and S. E. Razavi

Anti-mycobacterium activity from culture filtrates obtained from the dematiaceous fungus C10

March 2011

The aim of this work was to increase the concentration of substances with anti-mycobacterium activity in culture filtrates obtained from the dematiaceous fungus C10. An experimental design was employed to study the effect of glucose, potato infusion and Senna reticulata infusion. The anti-mycobacterium activity was determined by evaluating the growth of bacteria in culture medium containing “culture...

Author(s): João V. B. de Souza, Alita M. Lima, Eveleise S. de J. Martins and Julia I. Salem

Production of cellulase by different co-culture of Aspergillus niger and Penicillium chrysogenum from waste paper, cotton waste and baggase

February 2011

Cellulases are a group of hydrolytic enzymes capable of degrading cellulose to the smaller glucose units. These enzymes are produced by fungi and bacteria. The solid waste of sugar, paper and industry using baggase ans paper waste and cotton waste was fermented byAspergillus niger and Penicillium chrysogenum in solid state fermentation. There is attempts to transfer the various industrial carbon waste to...

Author(s): Jayant M ,Rashmi J, Shailendra M and Deepesh Y

Optimization of cellulase production by Aspergillus niger and Tricoderma viride using sugar cane waste

February 2011

Sugar cane waste was used as substrates for the production of cellulase enzyme usingAspergillus niger and Tricoderm aviride. From the fermentation studies, sugar cane waste gave the best result with an enzyme activity value of (using A. niger and T. aviride) 2.7 and 2.6 IU/ml respectively. It gave the maximum enzyme activity at about the 10 days of cultivation, suggesting that is the optimum...

Author(s): Ali A. Juwaied, Ahmed Abdulamier Hussain Al-amiery, Hamdan A. Adday and Umar Anaam

Morphology and taxonomy of Sarcoscypha ololosokwaniensis sp. nov.: A new Ascomycota species from Serengeti National Park-Tanzania

January 2011

Traditional taxonomy emphasizes the morphological features to characterize a taxon. Sarcoscypha is a genus in Sarcoscyphaceae family which display wide array of morphological variations. The genus is widespread in northern hemisphere and boreal regions, but also occurs in sub tropical areas and in the southern hemisphere. Both macro and micromorphological features including (ascocarp size, colour, shape, exterior...

Author(s): Donatha Damian Tibuhwa

Preparation and application of a novel environmentally friendly cucumber seed coating agent

January 2011

A novel cucumber seed coating agent was made from natural polysaccharide, fertilizer and microelement, etc., which achieved a good effect on increasing cucumber yield and antifeeding against pests. The foremost difference between this seed coating agent and conventional ones was that it controlled pests through the approach of repelling pests and enhancing the immunity of seeds, but not by killing pests. Results...

Author(s): Zeng Defang, Tu Renjie and Wu Shan

Dermatophytoses in rural school children associated with livestock keeping in Plateau state, Nigeria

January 2011

This article describes a study undertaken to evaluate the prevalence of dermatophytoses among one hundred and ten children between the ages of 3 to 14 years in rural primary school children of Barkin-Ladi Local Government Area, in association with the family livestock keeping, family hygiene level, environmental conditions of the schools and identify interventions to improve environmental health. Out of the number...

Author(s): I. D. Chukwu, O. O. C. Chukwu, Chuku A., Israel B., and B. I. Enweani 

Studies on the mycoflora associated with sugarcane factory waste and pollution of River Nile in upper Egypt

December 2010

Sixty-nine species and four varieties which belong to twenty eight genera of terrestrial fungi were recovered from polluted and nonpolluted water and mud samples on glucose and cellulose-Czapek's agar at 28ËšC. The most common species from the two substrates on the two types of media were Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium cladosporioides, Fusarium oxysporum, Mycosphaerella...

Author(s): A. H. M. El-Said, T. H. Sohair and A. G. El-Hadi

Antifungal activity of extracts obtained from actinomycetes

December 2010

In the present investigation an attempt has been done on isolation of actinomycetes from the soil, extracting the antifungal compounds from these isolated actinomycetes and then testing the extract against the growth of Alternaria sps, Aspergillus niger, Aspergillus flavus, Fusarium sps, and Rhizopus stolonifer. During the investigation it was found that nearly all the extracts were effective against the test fungi and...

Author(s): Harpreet Sharma and Leena Parihar

Improved antimicrobial activity of the Tanzanian edible mushroom Coprinus cinereus (Schaeff) Gray by chicken manure supplemented solid sisal wastes substrates

December 2010

The Tanzanian edible mushroom species Coprinus cinereus was grown on sisal waste substrates supplemented with chicken manure with the aim to evaluate the effects of the chicken manure supplement on the antimicrobial activity of the mushroom’s extracts. Crude ethyl acetate extracts were prepared from the mushroom’s fruiting bodies harvested at pre-capping, capping and post capping stages, and the...

Author(s): Liberata Nyang’oso Mwita, Anthony Manoni Mshandete and Sylvester Leonard Lyantagaye

FLAG tag module for PCR based gene targeting

November 2010

Epitope tagging of yeast proteins has become an efficient tool for biochemical analysis of protein of interest. The epitope-tagged proteins can be used for western blotting, immunoprecipitation and immunofluorescence experiments without the need to raise specific antibodies, thus saving considerable time and expense. We have constructed plasmid containing FLAG tag with kanMX6 module, which allows selection of...

Author(s): Rajeev Ranjan, Sumit Kumar Verma and Shakil Ahmed

Identification of Candida glabrata and Candida parapsilosis strains by polymerase chain reaction assay using RPS0 gene fragment

November 2010

Two Candida species were identified by the amplification of the RPS0 gene intron fragment. For this, two pairs of primers were used in PCR analysis performed with genomic DNA of clinical isolates of Candida. The primers designed are highly specific for their respective species and produce amplicons of the expected sizes and fail to amplify any DNA fragment from the other species tested. For Candida glabrata, the size of...

Author(s): Emira Noumi, Mejdi Snoussi, Maria del Pilar Vercher, Eulogio Valentin, Lucas Del Castillo and Amina Bakhrouf

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