African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12392

Full Length Research Paper

Co-expressing house fly cytochrome P450 6A1 and human cytochrome P450 reductase in Escherichia coli to bioconvert insecticide aldrin

Yating Wu
  • Yating Wu
  • Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South Central University for Nationalities, Wuhan 430074, PR China.
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Yong Li
  • Yong Li
  • Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South Central University for Nationalities, Wuhan 430074, PR China.
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Xiaosheng Liang
  • Xiaosheng Liang
  • Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South Central University for Nationalities, Wuhan 430074, PR China.
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Nan Jiang
  • Nan Jiang
  • Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South Central University for Nationalities, Wuhan 430074, PR China.
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Yunhua Wu
  • Yunhua Wu
  • Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South Central University for Nationalities, Wuhan 430074, PR China.
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  •  Received: 26 April 2018
  •  Accepted: 27 July 2018
  •  Published: 15 August 2018

Abstract

Aldrin used in agricultural as well as industrial or home pest control is known to be a persistent organic pollutant, having chronic adverse effects on wildlife and humans. The degradation of aldrin has drawn a lot of attentions. In this study, house fly cytochrome P450 6A1 (CYP 6A1) or both house fly cytochrome P450 6A1 and human NADPH-P450 reductase (CPR) together was expressed in Escherichia coli cells to study their activity on aldrin conversion. It was found out that the membrane fractions prepared from the recombinant E. coli cells expressing human CPR with CYP 6A1 have catalytic activity on aldrin using enzyme analysis. The catalytic activity of the recombinant E. coli cells was also investigated on aldrin. After adding aldrin to the co-expression of human CPR and CYP 6A1 recombinant E. coli cells culture with 1 µmol L-1 final concentration, 163.36 nmol L-1 of dieldrin was detected after 8 h, the product of aldrin epoxidation. These results indicate that co-expressing house fly CYP 6A1 and human CPR can convert aldrin to dieldrin.

 

Key words: Aldrin, bioconversion, cytochrome P450 6A1, cytochrome P450 reductase, dieldrin.