African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 406

Full Length Research Paper

Influence of the genetic polymorphism of haptoglobin in the occurrence of retinopathy and nephropathy in diabetics subjects

SAGNE René Ngor
  • SAGNE René Ngor
  • Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal.
  • Google Scholar
DJITE Moustapha
  • DJITE Moustapha
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
KANDJI Pape Matar
  • KANDJI Pape Matar
  • Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal.
  • Google Scholar
DIOP Jean Pascal Demba
  • DIOP Jean Pascal Demba
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
BARRY Nene Oumou Kesso
  • BARRY Nene Oumou Kesso
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
THIOUNE Ndeye Marieme
  • THIOUNE Ndeye Marieme
  • Laboratory of Biochemistry, University Hospital Fann, Dakar, Senegal.
  • Google Scholar
NDOUR El Hadji Malick
  • NDOUR El Hadji Malick
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
GUEYE-TALL Fatou
  • GUEYE-TALL Fatou
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
NDIAYE-DIALLO Rokhaya
  • NDIAYE-DIALLO Rokhaya
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
LOPEZ-SALL Philomène
  • LOPEZ-SALL PhilomeÌ€ne
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
CISSE Aynina
  • CISSE Aynina
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
DIOP Papa Amadou
  • DIOP Papa Amadou
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar
NDOUR-MBAYE Maimouna
  • NDOUR-MBAYE Maimouna
  • Department of Internal Medicine, Abass Ndao Hospital Center, Dakar, Senegal.
  • Google Scholar
GUEYE Papa Madieye
  • GUEYE Papa Madieye
  • Laboratory of Pharmaceutical Biochemistry, Faculty of Medicine, Pharmacy, Cheikh Anta Diop University, Dakar, Senegal.
  • Google Scholar


  •  Received: 27 October 2020
  •  Accepted: 11 January 2021
  •  Published: 31 January 2021

Abstract

The objective of this study was to determine the frequency of haptoglobin (Hp) genotypes and their association with nephropathy and retinopathy in diabetic subjects. This is a case-control study conducted in diabetic subjects developing nephropathy and/or retinopathy. Each patient was matched to a control of the same sex and the same age ± 2 years. Hp genotyping was performed by conventional PCR without enzymatic digestion on the Proflex® System PCR (Biosystems, Spain) and the biochemical parameters were determined using enzymatic techniques with the Cobas c311 system (Roche Diagnostics, Switzerland). The study population consisted of 60 diabetic subjects with an average age of 56 years and a sex ratio of 0.43. The Hp2-2 genotype was more frequent in diabetic subjects (40%) compared to control subjects (28.3%) (p= 0.18). The distribution of Hp1-1 and Hp2-1 genotypes in diabetic subjects showed rates of 46.7 and 13.3% respectively.  Multivariate analysis showed that the Hp 2-2 genotype was more associated with nephropathy (OR=1.77; p=0.8). These results showed an association between Hp polymorphism and diabetic microangiopathy. Thus, Hp2-2 genotype could be associated with a risk factor predisposing to the onset of diabetic kidney disease.

 

Key words: Polymorphism, gene, haptoglobin, diabetes, nephropathy, retinopathy.