African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5188

Full Length Research Paper

Bacterial communities associated with the surfaces of the fresh fruits sold around Dhaka Medical College and Hospital and their anti-microbial profiles

Kabir R. B.
  • Kabir R. B.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Zaman R.
  • Zaman R.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Tania N. E. J.
  • Tania N. E. J.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Md. Asaduzzaman
  • Md. Asaduzzaman
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Haque A.
  • Haque A.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Habib F. B.
  • Habib F. B.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Tanni N. N.
  • Tanni N. N.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Nesa M.
  • Nesa M.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Chowdhury A.
  • Chowdhury A.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Md. Rahman F.
  • Md. Rahman F.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Sarker A.
  • Sarker A.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Halder K.
  • Halder K.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Sharmin N.
  • Sharmin N.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Chowdhury M.
  • Chowdhury M.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Nahar S. S.
  • Nahar S. S.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Rahman M.
  • Rahman M.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Shahid S. B.
  • Shahid S. B.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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Shamsuzzaman S. M.
  • Shamsuzzaman S. M.
  • Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh.
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  •  Received: 13 October 2022
  •  Accepted: 20 December 2022
  •  Published: 31 January 2023

Abstract

Fresh fruits are popular sources of healthy diets with low energy density. Since they are consumed raw, it may act as a source of foodborne disease and a reservoir for antibiotic-resistant organisms. This study aimed to determine microbial prevalence among the fruits sold around hospital along with antimicrobial profiles. Thirty-five different types of fruits were bought from around Dhaka Medical College and Hospital (DMCH) and analyzed for the presence of bacteria. Antibiotic sensitivity, detection of ESBL, AmpC β-lactamase, and MBL positive strains were done by standard methods followed by PCR to detect ESBL, AmpC β-lactamase and MBL genes. Twenty-seven different organisms were isolated: Klebsiella spp. (33.33%), Citrobacter spp. (29.64%), Enterobacter spp. (22.22%), Escherichia coli (11.11%) and Staphylococcus aureus (3.70%). Among them, 48.15% were resistant to different antibiotics. Only one organism (Citrobacter spp.) produced ESBL phenotypically (7.69%). Two (15.38%) were positive for AmpC β-lactamase and one of these (Enterobacter spp.) possessed SHV and CTX-M15A genes by PCR. Imipenem resistance was 84.62% of the antibiotic-resistant organisms and 10 (90.91%) were phenotypically MBL positive. By PCR, one Enterobacter spp. had MBL encoding gene OXA-48.  Fresh fruits, contaminated with pathogens, might be a source of resistant organisms' transmission and contribute to public health issues.

 

Key words: Antibiogram, bacteria, Bangladesh, fresh fruits, fruit venders around hospital.