Abstract

Common pheasants have been most often breeded for sport hunting and for culinary meat. Lactic acid bacteria (LAB) are a part of the obligate intestinal microflora. They play important role in the development of the immune system. Concerning the common pheasants, only limited information exists in relation with LAB. The aim of our work was to study faecal lactobacilli isolated of 60 common pheasants (farm in west Slovakia) to the genus, Lactobacillus were allotted two strains of Lactobacillus brevis and two to Lactobacillus plantarum (50 isolates) identified by Maldi-Tof system expressed on the basis of high score values identification; confirmed by phenotypization and polymerase chain reaction. Undesirable enzymes β-glucuronidase and β-glucosidase showed negative reaction in L. plantarum LP13, L. plantarum LP14, L. brevis LB12. Amounts of lactic acid produced by strains were in accordance with typical representants of homofermentative lactobacilli. Strains inhibited the growth of at least of two out of 16 indicators; mostly listeriae. L. plantarum LP13 was the most active strain. LB12, LP13 and LP14 tolerate very low pH and 1% bile in growth medium. Results are contribution to the limited knowledge in this area; LP13 strain was selected for the further detailed antimicrobial studies.

Key words: Common pheasants, lactobacilli, identification, characterization.

Abbreviation

LAB, Lactic acid bacteria; CFU, colony forming unit; Maldi-Tof, matrix assisted laser desorption/ionization-time of flight mass spectrometry; mmo/l, millimol per liter; mm-millimeter; HSP, feed mixtures for animals; log10, logarithmus 10; nmol, nannomol; µg, microgram.