African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5087

Full Length Research Paper

Phytochemical screening and antimicrobial activity of Momordica charantia L. and Morinda lucida Benth extracts from Benin

Michaelle Chokki
  • Michaelle Chokki
  • Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d’Abomey-Calavi, 01BP: 526 ISBA Champ de foire, Cotonou Bénin.
  • Google Scholar
Cheikna Zongo
  • Cheikna Zongo
  • Laboratoire de Biochimie et immunologie Appliquées (LABIA), Ecole Doctorale Sciences et Technologies, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso.
  • Google Scholar
Durand Dah-Nouvlessounon
  • Durand Dah-Nouvlessounon
  • Department of Chemistry, Physics and Environment, Dunarea de Jos” University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
  • Google Scholar
Mihaela Cudălbeanu
  • Mihaela Cudălbeanu
  • Department of Chemistry, Physics and Environment, Dunarea de Jos” University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
  • Google Scholar
Pacôme Noumavo
  • Pacôme Noumavo
  • Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d’Abomey-Calavi, 01BP: 526 ISBA Champ de foire, Cotonou Bénin.
  • Google Scholar
Ioana Otilia Ghinea
  • Ioana Otilia Ghinea
  • Department of Chemistry, Physics and Environment, Dunarea de Jos” University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
  • Google Scholar
Bianca Furdui
  • Bianca Furdui
  • Department of Chemistry, Physics and Environment, Dunarea de Jos” University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
  • Google Scholar
Aly Savadogo
  • Aly Savadogo
  • Laboratoire de Biochimie et immunologie Appliquées (LABIA), Ecole Doctorale Sciences et Technologies, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso.
  • Google Scholar
Rodica Mihaela Dinica
  • Rodica Mihaela Dinica
  • Department of Chemistry, Physics and Environment, Dunarea de Jos” University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
  • Google Scholar
Lamine Baba-Moussa
  • Lamine Baba-Moussa
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05BP1604 Cotonou, Benin.
  • Google Scholar
Farid Baba-Moussa
  • Farid Baba-Moussa
  • Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d’Abomey-Calavi, 01BP: 526 ISBA Champ de foire, Cotonou Bénin.
  • Google Scholar


  •  Received: 24 April 2020
  •  Accepted: 29 July 2020
  •  Published: 31 August 2020

Abstract

Momordica charantia and Morinda lucida are Benin’s pharmacopeia plants that are used traditionally for the treatment of infectious diseases. This study aims to investigate phytochemical profile and antimicrobial activity of both plants. The dried leaf powder is used for extraction with different solvents by ultrasonication (35 Hz) at room temperature for 2 h. TLC and the method based on coloring and precipitation differential reactions were used for preliminary screening. HPTLC analysis was performed on silica gel 60 F254, 20.0 X 10.0 cm HPTLC plates, with Toluen: Ethyl acetate: Formic Acid: Methanol (3:4:0.8:0.7 v/v) as a mobile phase. The antibacterial and antifungal activities were assessed in vitro by the method of macrodillution and solid medium agar diffusion. TLC analysis showed many spots which suggest that both of the plants extracts contain various secondary metabolites. HPTLC revealed the presence of Quercetine, cafeic acid and vanilic acid in the plants’ extract. M. charantia extracts have shown the largest inhibition diameters (25.00±0.00 mm) and inhibit more strains than M. lucida extracts. From all the tested strains, only P. aeruginosa was the most sensitive to M. charantia extracts with 50% bactericidal effect.

 

Key words: Phytochemical screening, antimicrobial activity, Momordica charantia and Morinda lucida, Benin.