African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Côte d’Ivoire

Audrey A. Addablah
  • Audrey A. Addablah
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar
Solange Kakou-Ngazoa
  • Solange Kakou-Ngazoa
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar
Eric E. Akpa
  • Eric E. Akpa
  • Laboratoire de Pharmacodynamie Biochimique, Université Félix Houphouët-Boigny, 01 BP V34 Abidjan 01, Côte d’Ivoire.
  • Google Scholar
Emmanuella Adioumani
  • Emmanuella Adioumani
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar
Fred M’Bourou Ndombi
  • Fred M’Bourou Ndombi
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar
Serge Aoussi
  • Serge Aoussi
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar
Mireille Dosso
  • Mireille Dosso
  • Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar


  •  Received: 01 June 2021
  •  Accepted: 14 September 2021
  •  Published: 31 October 2021

Abstract

The application of bacteriophages biocontrol requires the formulation of genetically distinct bacteriophages in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered a cheap, reproducible, and readily applicable tool in detecting phage diversity compared to other molecular techniques such as whole-genome sequencing. We used in this study the RAPD-PCR technique to assess the genetic diversity of 28 bacteriophages infecting Pseudomonas aeruginosa and Staphylococcus aureus. According to their RAPD profiles, isolated phages were grouped into 2 main clusters which included phages from the same host. The typing by RAPD-PCR of newly isolated phages was useful to assess the genetic diversity bypassing previous whole-genome sequencing analysis. These genetically distinct phages lytic against P. aeruginosa and S. aureus could potentially be used in a phage cocktail for biocontrol against these clinically and industrially relevant bacteria.

 

Key words: Phages, genetic diversity, RAPD PCR, P. aeruginosa, S. aureus.