International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 103

Full Length Research Paper

Yeasts from a traditional ferment “Rabilé”: Molecular and physiological characterization

Ibrahim KEITA
  • Ibrahim KEITA
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako, BP: E 3206, Colline de Badalabougou, Bamako, Mali.
  • Google Scholar
Iliassou MOGMENGA
  • Iliassou MOGMENGA
  • Laboratoire de Microbiologie et Biotechnologie Microbienne (LAMBM), Université Joseph KI-ZERBO, 03 PB 7031 Ouagadougou 03, Burkina Faso.
  • Google Scholar
Marius Kounbèsiounè SOMDA
  • Marius Kounbèsiounè SOMDA
  • Laboratoire de Microbiologie et Biotechnologie Microbienne (LAMBM), Université Joseph KI-ZERBO, 03 PB 7031 Ouagadougou 03, Burkina Faso.
  • Google Scholar
Lassina DOUMBIA
  • Lassina DOUMBIA
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako, BP: E 3206, Colline de Badalabougou, Bamako, Mali.
  • Google Scholar
Aboubakar S. OUATTARA
  • Aboubakar S. OUATTARA
  • Laboratoire de Microbiologie et Biotechnologie Microbienne (LAMBM), Université Joseph KI-ZERBO, 03 PB 7031 Ouagadougou 03, Burkina Faso.
  • Google Scholar
Ousmane A. KOITA
  • Ousmane A. KOITA
  • Laboratoire de Biologie Moléculaire Appliquée (LBMA), Université des Sciences, des Techniques et des Technologies de Bamako, BP: E 3206, Colline de Badalabougou, Bamako, Mali.
  • Google Scholar
Alfred S. TRAORE
  • Alfred S. TRAORE
  • Laboratoire de Microbiologie et Biotechnologie Microbienne (LAMBM), Université Joseph KI-ZERBO, 03 PB 7031 Ouagadougou 03, Burkina Faso.
  • Google Scholar


  •  Received: 26 July 2021
  •  Accepted: 04 January 2022
  •  Published: 28 February 2022

Abstract

Rabilé” is a popular traditional ferment in Burkina Faso, consisting mainly of yeasts. It is used as a food supplement or additive like Single Cell Protein (SCP). The present work focused on identifying yeast microbiota in local food, and studying their growth kinetic parameters. “Rabilé” sampling from the 13 regions of Burkina Faso was used to isolate yeast strains. Molecular methods, including PCR-RFLP, Sanger Sequencing, and Single Locus Analysis, were applied for strain identification. The kinetic parameters were determined in batch culture. The results show 390 isolates belonging to 12 species with a predominance of Saccharomyces cerevisiae, followed by Cutaneotrichosporon curvatus. Among the selected strains, S. cerevisiae OG22 and Kluyveromyces marxianus KY01 showed the highest maximum growth rate (0.566 and 0.568 h-1) concerning kinetic parameters. These results demonstrate that “Rabilé” is an important biotope of yeast strains, and could be a potential food supplement.

 

Key words: Yeast, Rabilé, traditional ferment, molecular identification, kinetic parameters.