Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 298

Full Length Research Paper

The lupeol content in Machaerium species by HPLC-APCI-MS/MS and the allelopathic action

Luciana Sayuri Tahira
  • Luciana Sayuri Tahira
  • Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Campus leste, CEP 03828-000, São Paulo, Brazil.
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Robson Akito Tino
  • Robson Akito Tino
  • Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Campus leste, CEP 03828-000, São Paulo, Brazil.
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Charlyana Carvalho Bento
  • Charlyana Carvalho Bento
  • Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Campus leste, CEP 03828-000, São Paulo, Brazil.
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Marcelo Marucci Pereira Tangerina
  • Marcelo Marucci Pereira Tangerina
  • Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, CEP 05508-090, São Paulo, Brazil.
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Luiz Fernando Rolim de Almeida
  • Luiz Fernando Rolim de Almeida
  • Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho”, cp 610, CEP 18618-689, UNESP, Botucatu, São Paulo, Brazil.
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Danilo Miralha Franco
  • Danilo Miralha Franco
  • Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho”, cp 610, CEP 18618-689, UNESP, Botucatu, São Paulo, Brazil.
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Angela Lucia Bagnatori Sartori4
  • Angela Lucia Bagnatori Sartori4
  • Universidade Federal do Mato Grosso do Sul, Campo Grande- MS, CEP 79070-900, Brazil.
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Miriam Sannomiya
  • Miriam Sannomiya
  • Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Campus leste, CEP 03828-000, São Paulo, Brazil.
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  •  Received: 23 April 2021
  •  Accepted: 19 May 2021
  •  Published: 31 May 2021

Abstract

The use of extracts from plants and/or allelopathic compounds from natural products can minimize the use of pesticides in agriculture. This is a chemical study of Machaerium eriocarpum branch extract and the evaluation of the allelopathic potential of Machaerium hirtum (Vell.) Stellfed and M. eriocarpum Benth (Fabaceae) leaves and branches extracts. The triterpene lupeol was isolated from the hydroethanolic extract of M. eriocarpum branches. The quantification of this compound in all extracts was performed by HPLC-APCI-MS/MS and the highest amount of lupeol was observed in the leaves extract of M. eriocarpum. Allelopathy tests were carried out against seeds of Sorghum bicolor L. (sorghum), evaluating the final percentage of germination, germination speed index (GSI), root growth and number of lateral roots. The leaves extracts of both species were effective in the inhibition of the root growth as well as of the lateral roots. The branches extract of M. hirtum showed inhibitory effect in the root growth and lateral roots only in 50 and 100 mg.L-1 doses. The lupeol showed mainly a strong inhibition of seed germination, root growth and lateral root appearance at 100 μM. This study showed the potentiality of Machaerium extracts and lupeol as bioherbicides.

Key words: Allelopathy, lupeol, Machaerium, Fabaceae, Sorghum bicolor, phytoxicity