Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 160

Full Length Research Paper

Prevalence and characterization of Salmonella isolated from vegetables salads and ready to eat raw mixed vegetable salads in Abidjan, Côte d’Ivoire

Evelyne TOE
  • Evelyne TOE
  • UFR of Biological Sciences, Department of Biochemistry-Genetic, University Peleforo Gon Coulibaly, Korhogo, Côte d’Ivoire.
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Paul ATTIEN
  • Paul ATTIEN
  • Biochemistry-Microbiology Department, Agrovalorisation Laboratory, Jean Lorougnon GUEDE University, Daloa, Côte d´Ivoire.
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Aboya Jean-Luc MOROH
  • Aboya Jean-Luc MOROH
  • UFR of Biological Sciences, Department of Biochemistry-Genetic, University Peleforo Gon Coulibaly, Korhogo, Côte d’Ivoire.
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Haziz SINA
  • Haziz SINA
  • Laboratory Biology and Typing Molecular in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
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Désiré Nzébo KOUAME
  • Désiré Nzébo KOUAME
  • UFR of Biotechnologies, Biosciences Laboratory, Félix Houphouët Boigny University Abidjan Côte d’Ivoire.
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Ollo KAMBIRE
  • Ollo KAMBIRE
  • Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
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Lamine BABA-MOUSSA
  • Lamine BABA-MOUSSA
  • Laboratory Biology and Typing Molecular in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
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Nathalie GUESSENND
  • Nathalie GUESSENND
  • Laboratory of Biotechnology and Food Microbiology, Department of Food Science and Technology, University of Nanguy Abrogoua, Abidjan, Cote d’Ivoire.
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Etienne DAKO
  • Etienne DAKO
  • Laboratory of Biotechnology and Molecular Biology, School of Food Science, Nutrition and Family Studies, Faculty of Health Science and Community Services, University of Moncton, Canada.
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Adjehi T. DADIE
  • Adjehi T. DADIE
  • UFR of Biological Sciences, Department of Biochemistry-Genetic, University Peleforo Gon Coulibaly, Korhogo, Côte d’Ivoire.
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  •  Received: 18 October 2021
  •  Accepted: 04 January 2022
  •  Published: 31 January 2022

Abstract

Raw vegetables have been linked to many outbreaks of Salmonella foodborne; however there is few data on the presence of this bacteria in raw vegetables in Côte d'Ivoire. The objective of this study is to determine the prevalence, diversity and antibiotic resistance level of Salmonella strains in vegetables salads and ready-to-eat raw mixed vegetable salads in Abidjan. From a total of 552 samples, Salmonella strains were biochemically and molecularly identified by detection of the 16S rRNA gene and serotyping with specific antisera. The antibiotic resistance level was phenotypically determined by disc diffusion method and the presence of the gene encoding for resistance was determined by PCR. The prevalence of Salmonella spp in vegetables salads and ready-to-eat raw mixed vegetable salads was 8.54 and 2.61%, respectively. The serotypes identified were S. typhimurium, S. enteritidis, S. selby, S. hadar, S. typhy, S. paratyphi C and S. adamstown. It was observed that there were non-resistant (tetracycline and streptomycin) and multiresistant (nalidixic acid and ciprofloxacin) strains. Genes, such as tetA, tetB, aaa [3] -IV and QnrA were highlighted at different proportion. Vegetable’s salads and ready-to-eat raw mixed vegetable salads in Abidjan contain various serotypes of Salmonella spp. displaying resistant to antibiotics and harboring the genes encoding for resistance. It is important to make subsequent risk control to evaluate and prevent possible food poisoning.

 

Key words: Salmonella, vegetables salads, Abidjan, prevalence, antibiotic resistance.