African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5124

Full Length Research Paper

Virulence and multi-resistance of gram-negative bacilli strains isolated from some artisanal fermented dairy products sold in secondary schools in Benin

Majoie Géroxie TOHOYESSOU
  • Majoie Géroxie TOHOYESSOU
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Cotonou, Benin.
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Wassiyath MOUSSE
  • Wassiyath MOUSSE
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Cotonou, Benin.
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Chimène NANOUKON
  • Chimène NANOUKON
  • Laboratory of Molecular Biology and Bioinformatics Applied to Genomics, National High School of Biosciences and Applied Biotechnologies of Dassa, National University of Sciences, Technologies, Engineering and Mathematics of Abomey, Dassa-Zoumè, Benin.
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Haziz SINA
  • Haziz SINA
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Cotonou, Benin.
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Akim SOCOHOU
  • Akim SOCOHOU
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Cotonou, Benin.
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Edwige AKA
  • Edwige AKA
  • Microbiological Resources Management Unit (UGMI), Pastor Institute of Ivory Coast, 01 BP 490 Abidjan 01, Ivory Coast.
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Rodrigue TOLLO
  • Rodrigue TOLLO
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Cotonou, Benin.
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Farid BABA-MOUSSA
  • Farid BABA-MOUSSA
  • Laboratory of Microbiology and Food Technology, Department of Plant Biology, Cotonou Benin, University of Abomey-Calavi, Benin.
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Adolphe ADJANOHOUN
  • Adolphe ADJANOHOUN
  • Benin National Institute of Agricultural Research, Cotonou, Benin.
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Lamine BABA-MOUSSA
  • Lamine BABA-MOUSSA
  • Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Cotonou, Benin.
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  •  Received: 11 January 2021
  •  Accepted: 19 April 2021
  •  Published: 30 April 2021

Abstract

This work aims at assessing toxin production capability and antibiotic resistance profiles of thermo-tolerant Gram-negative bacilli strains isolated from three types of fermented dairy products (yoghurt, dèguè millet and dèguè couscous). Samples collected in Abomey-Calavi and Cotonou were analyzed for microbial, biochemical and molecular parameters. Samples were contaminated with thermo-tolerant Gram-negative bacilli strains at 13.88%. The high contamination rate was recorded with the samples of dèguè couscous and the lowest contaminated samples were dèguè millet. Morning samples were more contaminated. Klebsiella pneumoniae, Enterobacter aerogenes, Escherichia coli and Enterobacter cloacae were the most identified bacteria. The most isolated species in the dry season was E. coli. In the rainy season, K. pneumoniae and E. cloacae were the most isolated species. K. pneumoniae was the most formative of biofilm (38.9%). About 12% of the isolated strains were extended-spectrum beta-lactamases (ESBL) producers. The higher resistance rate was observed with amoxicillin and doxycylin (92.3%). Imipenem was the most efficient molecule on the isolated strains with 00% of resistance. The blaTEM gene was detected in 51.43% of the isolated strains followed by blaSHV (37.14%), blaCTX-M (8.57%) and blaOXA-1 (2.86%). It is necessary to train sales people on good hygiene practices for food during their production and their distribution.
 
Key words: Fermented milk products, thermo-tolerant gram-negative bacilli, toxins, antibiotics resistance, Benin.