Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 271

Full Length Research Paper

Changes in flavonoids concentration of Hawthorn (Crataegus pinnatifida) in response to exogenous amino acids

Cui Yu*
  • Cui Yu*
  • Industrial Crops Institute of Hubei Academy of Agricultural Sciences, 430064 Wuhan, China.
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De-Guo Lv
  • De-Guo Lv
  • College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China.
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Xing-Ming Hu
  • Xing-Ming Hu
  • Industrial Crops Institute of Hubei Academy of Agricultural Sciences, 430064 Wuhan, China.
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Wen Deng
  • Wen Deng
  • Industrial Crops Institute of Hubei Academy of Agricultural Sciences, 430064 Wuhan, China.
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  •  Received: 30 September 2015
  •  Accepted: 27 October 2015
  •  Published: 30 October 2015

Abstract

Hawthorn (Crataegus pinnatifida Bge.) flavonoids constituents have recently received greater attention for their health and medical properties. In order to relgulate the  flavonoids constituents, one-year-old hawthorn plants were subjected to foliar application of L-glutamic acid (GLA, 800 mg L–1 ), glycine (GLY, 20 mg L–1), L-phenylalanine (PHE, 20 mg L–1), L-arginine (ARG, 20 mg L–1). The levels of rhamnosylvitexin, rutin and hyperoside in both leaves and root exudates were significantly (p<0.05) increased in GLA and PHE treatments. Application of GLY and PHE increased the levels of rutin and hyperoside in hawthorn roots, especially for PHE treatment, and the level of hyperoside in PHE treatment was significantly (p<0.05) higher than in other treatments. No positive effects were observed for quercetin under exogenous amino acids treatments in both leaves and roots. The total flavonoids content of leaves, roots and root exudates under GLA and PHE treatments were significantly increased compared to the control. In addition, the phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activities of leaves and roots were also increased. These results suggest that some exogenous amino acids did affect the flavonoids content, which may have significance in regulation of flavonoids in hawthorn.
 
Key words: Amino acids, flavonoids, phenylalanine ammonia-lyase.