International Journal of
Genetics and Molecular Biology

  • Abbreviation: Int. J. Genet. Mol. Biol.
  • Language: English
  • ISSN: 2006-9863
  • DOI: 10.5897/IJGMB
  • Start Year: 2009
  • Published Articles: 126

IJGMB Articles

Genetic analysis of twenty two selected genotypes of Jatropha curcas L. (physic nut) from Africa, Asia and America, using SSR and AFLP markers

July 2020

Twenty two genotypes of J. curcas L. from Africa (Senegal, Burkina Faso, Mali, Congo and Madagascar Island), Asia (Cambodia, China and India) and America (Ecuador, Dominican Republic and Brazil) selected for their vigor and their productivity were analyzed with ten SSR primer pairs and six AFLP primer combinations. The two marker approaches showed their ability to effectively reveal polymorphism among the selected...

Author(s): N’Guessan Olivier KONAN, and Guy MERGEAI  

Impact of glutathione S-transferase genes polymorphisms on human papillomavirus infection and precancerous lesions in West African women

July 2020

Genetic polymorphisms of certain classes of glutathione S-transferase (GST), enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with risk of several cancers such as cervical cancer. The aim of this study is to investigate the impact of glutathione S-transferase M1 and T1 deletion on high-risk human papillomavirus (HR-HPV) infections and on dysplasia. A case-control study was...

Author(s): Teega-Wendé Clarisse OUEDRAOGO, Florencia Wendkuuni DJIGMA, Boureima IDANI, Théodora Mahoukèdè ZOHONCON, Pegdwendé Abel SORGHO, Prosper BADO, Mah Alima Esther TRAORE, Abdoul Karim OUATTARA, Marius Ayaovi SETOR, Shoukrat Ohuwa Toyin BELLO, Simplice Damintoti KAROU, Apollinaire HORO, Kouame Privat KOUAKOU, Moutawakilou GOMINA, Mady NAYAMA, D. Callinice CAPO-CHICHI, Ambaliou SANNI, Dorcas OBIRI-YEBOAH, Simon AKPONA, Albert Théophane YONLI, Charlemagne OUEDRAOGO and Jacques SIMPORE,  

Morphological diversity patterns among selected elite Shea trees (Vitellaria paradoxa C.F. Gaertn.) from Tchologo and Bagoué districts in Northern Côte d’Ivoire

February 2020

Agromorphological diversity structure of the elite shea trees identified in village lands and conserved in situ in the districts of Bagoué and Tchologo by the shea breeding program of the University of Peleforo Gon Coulibaly (UPGC, Côte d’Ivoire), are not known. In the present study, we characterized the agromorphological parameters of 220 elite shea trees using a set of 12 quantitative traits. The...

Author(s): YAO Saraka Didier Martial, DIARRASSOUBA Nafan, ATTIKORA Affi Jean Paul, FOFANA Inza Jesus, DAGO Dougba Noel and SILUE Souleymane  

Mapping of fourteen high-risk human papillomavirus genotypes by molecular detection in sexually active women in the West African sub-region

February 2020

The aim of this study was to determine the distribution of high-risk human papillomavirus genotypes (HR-HPV) in women from the general population of five West African countries. This was a cross-sectional descriptive study, involving 2133 women from nine cities of five West African countries: Benin, Burkina Faso, Côte d'Ivoire, Niger and Togo. Women were screened for precancerous cervical lesions and HR-HPV...

Author(s): Théodora Mahoukèdè Zohoncon,, Wendkuuni Florencia Djigma,, Abdoul Karim Ouattara, Ina M.A Traore, R. Alice Ouedraogo, Esther M.A Traoré, Prosper Bado, T. Clarisse Ouedraogo, Birama Diarra, Maimouna Ilboudo, Callinice D. Capo-chichi, Dorcas Obiri-Yeboah, Simplice Damintoti Karou, Mady Nayama, Apollinaire Horo, Kouame Privat Kouakou, Moutawakilou Gomina, Souleymane Ouattara, Ambaliou Sanni, Akpona Simon, Charlemagne Ouedraogo, and Jacques Simpore,,  

Distribution of groundnut rosette disease and sequence diversity of groundnut rosette virus associated satellite RNA (Sat-RNA) in Western Kenya

February 2020

Production of groundnuts (Arachis hypogaea L.) in Western Kenya is mainly constrained by groundnut rosette disease (GRD) which cause up to 100% yield loss. This disease expresses different symptoms as a result of variations in the groundnut rosette virus (GRV) associated satellite-ribonucleic acid (GRV Sat-RNA). Over the past 20 years, no work had been done to document the status of the disease in Kenya. Additionally,...

Author(s): Benard Mukoye, Anthony Simiyu Mabele, Millicent Florence Owuor Ndonga, Bonphace Collins Mangeni and Hassan Karakacha Were  

First detection of the hemizygote frameshift variant of the DMD gene in a 13-year-old patient affected by dystrophinopathy (Duchenne DMD and Becker BMD) at Saint Camille Hospital of Ouagadougou, Burkina Faso: Case report

February 2020

Genetic diseases are poorly reported in sub-Saharan Africa, especially in Burkina Faso. The reasons for this reality are multifactorial including, the difficulty of diagnostic confirmation, financial accessibility and even the difficulty with referral of cases by medical staff. Genetic diseases, although relatively rare, exist in families and deserve special attention in our paraclinical assessments. Few cases can be...

Author(s): Théodora Mahoukèdè Zohoncon,, Paul Ouedraogo, Marius Belemgnegre, Abdoul Karim Ouattara, L. Bienvenue Tingueri  and Jacques Simpore,,

Verification of single nucleotide polymorphism (SNP) markers associated with maize (Zea mays. L) streak virus resistance in early generation maize lines

February 2020

Maize streak virus (MSV) is a devastating viral disease of maize in sub-Saharan Africa. The disease may cause up to 100% yield loss in susceptible crops. The use of molecular markers can facilitate the development of varieties resistant to the virus. The objective of this study is to assess the usefulness of Single Nucleotide Polymorphism (SNP) markers linked to MSV resistance in selecting for resistance at early...

Author(s): Solomon Shibeshi, V.O. Adetimirin, Lava Kumar, Menkir Abebe and Melaku Gedil

Characterisation of three morphotypes of Solenostemon rotundifolius [(Poir.) J. K. Morton] cultivated in Burkina Faso using quantitative traits

December 2019

Evaluation of intra specific variability is a key step toward conservation and sustainable use of species. This study was carried out to describe the morphotypes of Solenostemon rotundifolius (Lamiaceae) based on quantitative traits. Three accessions (E02, E35 and E20), representing the morphotypes “A”, “B” and “C” were characterised in Randomised Complete Block Design with three...

Author(s): Romaric Kiswendsida NANEMA, Zakaria KIEBRE, Renan Ernest TRAORE, Aminata Hamidou BA and Francis KUSI

Primary morphological characterization of West African dwarf (Djallonké) ewes from Côte d’Ivoire based on qualitative and quantitative traits

December 2019

Phenotypic characterization is used to identify and document diversity within and between distinct breeds, based on their observable attributes. Study to assess the body characteristics and variability of 204 Djallonké (West African dwarf) ewes was conducted in three agro-ecological zones (central, western and northern) of Côte d’Ivoire, from November 2016 to April 2017. Animals were described using...

Author(s): N’Goran K. Edouard, Kouadja G. Severin, Kouassi N. Cyrille, Loukou N. Etienne, Eka Jean Yves, Dayo G. K. Charles, Sangare Mamadou and Yapi-Gnaore C. Valentine

Assessment of radio-sensitivity for three cowpea genotypes to gamma irradiation

December 2019

Cowpea (Vigna unguiculata [L.] Walp) is an important food legume. However, production is limited by various biotic and abiotic constraints in sub-Saharan Africa and reduced genetic variability hinders crop improvement efforts. Gamma ray irradiation has been used as a mutagen to create variability but optimal dose determination is a prerequisite to maximize the recovery of mutant plants. In this study, three genotypes...

Author(s): Karidiatou Gnankambary, Teyouré Bénoît Joseph Batieno, Nerbéwendé Sawadogo, Mahamadou Sawadogo, Djibril Yonli and Tinga Jeremy Ouédraogo

Coexistence between (TOHO-type and BES-type) extended-spectrum β-lactamase genes of identified enterobacteria at Saint Camille Hospital, Ouagadougou, West Africa

December 2019

Coexistence between the TOHO-type and Brazilian extended-spectrum (BES)-type of extended-spectrum β-lactamase (ESBL)-produced by bacteria caused public health issue. Several studies have been reported on the coexistence between blaTEM, blaCTX-M and blaSHV genes in ESBL in broad spectrum enterobacteria. The present study involved the prevalence of coexistence of blaTOHO and blaBES genes in enterobacteria identified...

Author(s): Mètuor-Dabiré Amana, Sougué Serge, Tiemtoré Rahimatou Yasmine Wend-kuni, Zohoncon Théodora Mahoukèdè, Bangré Yasmine Aminata, Ouédraogo Paul, Kabré Elie and Simporé Jacques,

Inheritance of maize (Zea mays L.) leaf traits

December 2019

Maize leaf plays an important role in the synthesis of organic compounds. It hosts the interception of light energy and conversion to organic energy, and the capture and assimilation of carbon dioxide in order to produce food. In this study, we explore the heritability of the maize leaf length, leaf width, and the number of leaves of two maize genotypes, JNE and BLC. The estimates of heritability varied from 0.2 to...

Author(s): Seka, D., Bonny, B. S., Adjoumani, K., Alla Eby, Y. G. H., Yoboué, A. N. ,  Sié, R. S. and Adepo-Gourene B.A.  

Prevalence and genetic diversity of Plasmodium falciparum in patients attending regional health center in Daloa, Côte d'Ivoire

December 2019

Malaria is a parasitic disease defined as a major problem of public health in Côte d'Ivoire. One of the constraints of the control of this disease might be the genetic diversity of the main pathogen, Plasmodium falciparum. This study aims to evaluate the genetic diversity of Plasmodium falciparum in individuals at the regional health center in Daloa, Côte d’Ivoire. A total of 380 blood samples were...

Author(s): Mathurin Koffi, Innocent Allepo Abe, Bernardin Ahouty Ahouty, Dieudonné Silué, Thomas Konan Konan and Abiba Sanogo Tidou

Investigation of polymorphisms of exon 1 region of OsHKT1;5 gene in high yielding rice

June 2019

This study was carried out to analyze the polymorphic region of exon 1 of OsHKT1;5 of 22 high yielding rice varieties that are salt tolerant. The result of the phenotypic screening for salinity stress was done using the standard protocol of the International Rice Research Institute which recorded that all rice varieties developed very well under control condition, 85.38% were quite tolerant to salinity treatment of...

Author(s): Nguyen Thi Pha, Nguyen Thi Phung, Nguyen Thi Ngoc Truc, Do Tan Khang and Tran Dinh Gioi

The characterization of Citrus sp. from Parang Island Karimunjawa based on morphological, DNA barcoding and nutritional analysis

September 2018

The Citrus sp. from Parang Island Karimunjawa is a wild type of Citrus plant that grow on salt area and exhibits a high level of vitamin C.  Due to its ability, morphological, molecular and nutritional characterization needs to be carried out to improve its potential. The study was carried out at the Biotechnology Laboratory and the Integrated Laboratory of Diponegoro University from 2016 to 2017. The...

Author(s): Hermin Pancasakti Kusumaningrum, Anto Budiharjo, Agung Suprihadi, Yuriza Eshananda, Annisa Fadillah and Dina Rahayuning Pangestuti  

Use of expressed sequence tags-derived simple sequence repeat (SSR) markers for population studies of released and elite sweet potato

April 2018

Development of improved varieties of sweet potato to meet specific end users in the sub-Saharan African region has the potential of transforming the crop’s market, consumption and health benefits. Molecular biology techniques such as fingerprinting are key to crop improvement, and critical for numerous genetic studies. This study, carried out in Ghana, seeks to determine genetic relatedness among two populations...

Author(s): Marian Dorcas Quain, Kwadwo Adofo, David Appiah-Kubi, Ruth Naa Prempeh, John Asafu-Agyei, Belinda Akomeah and Harrison Dapaah

Unravelling genetic make-up of some Musa hybrids and selected Musa accessions using molecular and morphological characterization

March 2018

Plantains are important staples in sub-Saharan Africa, however, production has been saddled by several constraints including pests and diseases and lack of improved technologies. In regard to genetic study, morphological characterization has identified about 100 local accessions to exist in Ghana. However, limited molecular characterization has been done on plantains. Characterization based on morphologic...

Author(s): Marian D. Quain, Adelaide Agyeman, Emmanuel Okyere and Beloved M. Dzomeku  

De novo assembly and characterization of transcriptome and microsatellite marker development for Taro (Colocasia esculenta (L.) Schott.)

December 2017

Taro (Colocasia esculenta. (L.) Schott) is a genus of perennial plants that is widely distributed in the tropics or subtropics of Asia, Africa and America, which is the fourteenth most consumed vegetable of the world. However, molecular genetic research of Colocasia has been hindered by the insufficient genomic and transcriptome information. Here, the transcriptome of taro variety ‘Jingjiang Xiangsha’ from...

Author(s): Li Wang, Jianmei Yin, Peitong Zhang, Xiaoyong Han, Wenqi Guo and Chunhong Li

Broad sense heritability and genetic advance for grain yield and yield components of chickpea (Cicer arietinum L.) genotypes in western Ethiopia

November 2017

Genetic variability is a prerequisite to crop improvement. The objective of the present study was to assess and quantify the genetic variability, estimate heritability and genetic advance for yield and yield contributing characters of released and pipeline chickpea varieties based on agro- morphological traits. A total of 16 chickpea materials (8 improved varieties, 7 advanced lines, and one local check) were planted in...

Author(s): Biru Alemu, Kassahun Tesfaye,, Teklehaimanot Haileselassie and Dagnachew Lule

Genetic polymorphism of vesicular monoamine transporter 1 gene (SLC18A1) in Emirati population

May 2017

Vesicular monoamine transporters (VMATs) are an important target for biological research in neuropsychiatric disorders. Recent studies indicated that VMAT1 is expressed in the brain, thus making transporter plausible candidate genes for neuropsychiatric disorders. Furthermore, several recent genetic case-control studies have documented an association between common missense variations in the VMAT1 gene and...

Author(s): Abdullah Al Mutery, Walaa Kamal, Hawraa Abduljalil Hussein Al Bloushi, Mary Sebastian Manjuran and Naushad Rais

Biodiversity of uncultured bacteria in hypersaline lakes, Siwa oasis, Egypt, as determined by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes

April 2017

Bacterial populations within hypersaline lakes often exhibit uncultured unique species. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), followed by sequencing of 16S rRNA gene approach was applied in order to explore, for the first time, the bacterial communities within the hypersaline Lakes, Aghormy, Zeiton and Maraqi, Siwa Oasis, Egypt. The DGGE profile displayed 12 phylotypes of 16S rRNA...

Author(s): Hosam E. Elsaied, Mohamad Saad Abd El-Karim and Mai A. Wassel

The cichlid 16S gene as a phylogenetic marker: Limits of its resolution for analyzing global relationship

March 2017

The phylogenetic utility of the 16S gene in cichlids is assessed. Eighty-six (86) partial sequences belonging to 37 genera of cichlids from the Genbank was analyzed. The alignment had four hundred and sixty three (463) basepairs with 337 conserved sites and 126 variable sites. Base compositional bias is similar to that found in higher organism with Adenine having the highest average of 30.3%, followed by cytosine,...

Author(s): Olusola B. Sokefun

Genetic diversity analysis in South African taro (Colocasia esculenta) accessions using molecular tools

August 2016

Taro [Colocasia esculenta (L) Schott] belongs to the family Araceae. It is an important staple food crop grown mainly by small scale farmers in many parts of the world. Taro is grown in South Africa from the coastal parts of the northern Eastern Cape to the KwaZulu-Natal north coast. Although it is an important staple in South Africa, very little information exists on the genetic diversity of the crop. Knowledge of the...

Author(s): Mwamba Nguluta, Patrick Adebola and Michael Pillay

Comparison of Lagostomus maximus amelogenin gene (AMELX) with other mammals

August 2016

The aim of this study was to compare the Lagostomus maximus AMEL sequence to assess their similarity with the AMEL genes from other mammals. Previously, a sequence of the AMEL gene of L. maximus which corresponds to the intron 3 of the human sequence for AMELX (AMEL on the X chromosome) was reported. The comparisons made, by the use of the algorithm provided by the NCBI (Basic Local Alignment Search Tool: BLAST) allowed...

Author(s): María Beatriz Espinosa

Recurrent mutation in the HMGCL gene in a family segregating HMG-CoA lyase deficiency

July 2016

The gene HMGCL encodes 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase. Mutations in HMG-CoA lyase cause HMG-CoA lyase deficiency (HMGCLD), which is an autosomal recessive congenital disorder of metabolism. This study was designed to detect mutation in the 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL) gene in a Saudi family segregating HMG-CoA lyase deficiency (HMGCLD).Methods: Clinical and molecular genetic analysis of a...

Author(s): Essa Alharby, Omhani Malibari, Hamad S. Al-Otaibi, Muhammad Saud, Ghadeer Al-Harbi, Mohammad I. Samman, and Sulman Basit

Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria

May 2016

The present study was conducted to characterize the genetic diversity of 10 germplasm accessions of finger millet (Eleusine coracana (L) Gaertn) collected from diverse locations spread across the geographical zone of Northern Nigeria.  The digest was carried out using the molecular marker Restriction Fragment Digest (RFD on agarose gel while EcoR1 and Hind III restriction enzymes were used to cut the genomic DNA at...

Author(s): Umar I. D., Kwon-Ndung E. H. and Mailafia S.

Association of the extracellular superoxide dismutase Ala40Thr polymorphism with type 2 diabetes mellitus and its complications in Lebanese population

January 2016

Free radical-induced damage plays a significant role in the development of type 2 diabetes and its complications. Extracellular superoxide dismutase (EC-SOD) or SOD3 is one of the important scavenger enzymes that protects against oxidative damage and the formation of free radicals. EC-SOD is a copper- and zinc-containing secretory enzyme located in tissues and primarily in the extracellular matrix. EC-SOD has been...

Author(s): Nahed Saad, Mohamed E. Moustafa, and Hala A. Chamieh,

DYS459, DYS391, DYS388 and DYS19 genetic loci have high allelic frequency in patients with prostate cancer

August 2015

Prostate cancer is a significant disease in men, and a large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be established, which could aid in the early detection of the disease which is crucial for successful treatment. The first objective of this study was detection of allele frequencies of 12 Y-chromosome short tandem repeat loci from Iraqian...

Author(s): Hasanain Khaleel Shareef, Noran Jameel Ibraheem, Imad Hadi Hameed and Muhanned Abdulhasan Kareem

Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

July 2015

Alleviation of doxorubicin (DOX)-induced cytotoxicity and genotoxicity by leaf extract of bael also known as Aegle marmelos (AME) was studied in cultured Chinese hamster V79 cells. The optimum protective dose of AME was determined by treating V79 cells with different concentrations of AME before exposure to 10 µg/ml DOX and then by evaluating the cell survival and micronuclei frequency in the cytokinesis blocked...

Author(s): Ganesh Chandra Jagetia and Ponemone Venkatesh

Estimation of genetic parameters for first-lactation test-day milk yield in Holstein Friesian cows fitting random regression models

June 2015

In this study, the effects of varying order of Legendre polynomials (LP) for permanent environmental (Pe) variance structure on the estimates of genetic parameters for first-lactation milk yield were evaluated fitting random regression (RR) test-day animal models. The total data set included 6850 test-day milk yield records from 800 first-lactation Holstein Friesian cows that calved between 1997 and 2013 and the...

Author(s): Meseret S., Tamir B. and Negussie E.

Isolation and sequence analysis of a putative MerR-type-transcriptional regulator and a multidrug efflux protein of Bacillus circulans ATCC 21588: As potential targets of therapeutics

May 2015

Mercury-type transcriptional regulators (MerR-transcriptional regulator) and major facilitator superfamily (MFS) transporters usually form an important sensor-response transport system in many microorganisms. This system has been shown to be involved in the regulation and transport (efflux) of a wide and diverse array of secondary metabolites including antimicrobial agents, dyes, chemicals, metals and evem harmful...

Author(s): Khaled Mohamed Anwar Aboshanab and Mostafa Mahmoud Elshafey

Effects of cytokinin types and their concentration on in vitro shoot induction and multiplication of korarima

April 2015

Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a herbaceous perennial plant that belongs to the family Zingiberaceae in which lack of a steady supply of quality planting material is one of the bottlenecks for the exploitation of the export potential. Thus, the use of micropropagation is suggested to alleviate these problems. In micropropagation, in vitro seed germination and multiplication of hypocotyls/shoot...

Author(s): Rahiel Hagos and Hailay Gebremdhin      

Evidence of over-dominance for sickle cell trait in a population sample from Buenaventura, Colombia

February 2015

Sickle cell disease is a hereditary disease caused by a hemoglobin variant, known as hemoglobin S. This hemoglobin variant has a high frequency in areas where malaria is prevalent, because heterozygous genotype has a protective action against malaria infection. We evaluated evidence of over-dominance or deviation from Hardy- Weinberg equilibrium for the HbS gene in Buenaventura, an afro-descendent population with actual...

Author(s): Diana Carolina Ortega Cristian Fong, Heiber Cárdenas and Guillermo Barreto

Multivariate analysis of genetic divergence among Ethiopian mustard (Brassica Carinata A. Braun) landraces in Ethiopia

November 2014

Characterization of existing genetic variability is a prerequisite for further crop improvement activity. This study was designed to assess genetic variability among randomly selected Ethiopian mustard (Brassica Carinata A. Braun) genotypes from different agro ecology of Ethiopia. The present study was undertaken to determine nature of association of agronomic traits of 36 Ethiopian mustard (Brassica carinata) genotypes...

Author(s): Tesfaye Walle Mekonnen and Adugna Wakjira

Genetic polymorphism of blood potassium in goat belonging to the different breeds in Mongolia

November 2014

In goats belonging to the different breeds and sub breed, the genetic polymorphism at the determinant locus of blood potassium was revealed by flame spectrophotometer method. The kalemic systems in those breeds were characterized by a polymorphism of middle level due to the existence of the two phenotypes and of three genotypes. The polymorphic character of this system is given by the distributional discontinuity of...

Author(s): Myagmarsuren Purevdorj, Battsetseg Badgar, Dungu Dorjsuren and Myagmardulam Uherch

Use of simple sequence repeat (SSR) markers to establish genetic relationships among cassava cultivars released by different research groups in Ghanaian

October 2014

Cassava (Manihot esculenta) is an important staple crop widely cultivated in Ghana. The crop also has diverse industrial applications including starch, beer and alcohol productions. Knowledge about the state of the Ghanaian cassava genetic diversity and population structure is paramount in breeding programmes aimed at cultivar improvements or breeding of new cultivars for specific purposes. This study focused on the use...

Author(s): Peter Twumasi, Eric Warren Acquah, Marian D. Quain and Elizabeth Y. Parkes

Heliclobacter pylori associated chronic gastritis: Endoscopic and pathological findings, comparative study

March 2014

Helicobacter pylori have been established as a major etiologic factor in the pathogenesis of chronic gastritis, peptic ulcer disease and in the development of gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue lymphoma. This study was conducted on 100 patients. All underwent upper endoscopy, and antral and corpus, and duodenal biopsies were taken. Findings of endoscopic gastritis were observed in 83...

Author(s): Awwad K. A. Alenezy and Taha M. M. Hassan

Challenges in conserving and utilizing plant genetic resources (PGR)

March 2014

The problems of food and income security are of global significance and are further compounded by precedential increase in world population resulting in overexploitation of natural resources and by extension plant genetic diversity. Plant genetic resources (PGR) refer to the heritable materials contained within and among plant species of present and potential value. In the recent past, genetic diversity found in...

Author(s): Ogwu, M. C., Osawaru, M. E. and Ahana, C. M.

Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria

January 2014

Solanum L., the largest genus of the Solanaceae family, vary morphologically, is diverse in number and is ecogeographically distributed. In Nigeria, previous studies had focused mainly on chromosome morphology, genome description and medicinal values, which are insufficient for genetic affinities. This study used four highly polymorphic random amplified polymorphic DNA primers to describe both the genetic relatedness...

Author(s): Sifau, Mutiu Oyekunle, Ogunkanmi, Liasu Adebayo, Adekoya, Khalid Olajide, Oboh, Bola Olufunmilayo and Ogundipe, Oluwatoyin Temitayo

Variability assessment of seed traits in Jatropha curcas L. for improvement of oil yield

January 2014

Jatropha curcas has received considerable attention from researchers as a potential source of non-edible vegetable oil which is eminently suitable for production of liquid bio fuel, meeting international standards. For any tree improvement and breeding programme, study of variation among the populations is prerequisite as it helps in the detection of relative performance of various traits of economic value. 28 candidate...

Author(s): Sanjeev Kumar and Sanjay Singh

Insilico docking studies and evaluation of volatile oil of Zingiber officinale Rosc. and Aripiprazole for antidepressant activity

December 2013

The objective of the study was to evaluate the volatile oil of Zingiber officinale Rosc. and Aripiprazole for antidepressant activity and to carry out the docking studies for their respective binding sites. Argus Lab 4.0.1 software (Mark A. Thompson, Planaria Software   LLC, and Seattle, WA, USA) was used to perform the docking studies and the elevated plus maze, forced swim...

Author(s): Sibi P. Ittiyavirah and Meera Paul

Application of multiple displacement amplification in detection of five events of genetically modified organisms

December 2013

In this study, multiple displacement amplification (MDA) was used for transgene detection, and proved to effectively improve the sensitivity of most fluorescence polymerase chain (PCR). Five events of genetically modified plants were chosen for detection in different plant species by MDA and real-time PCRs. Individual primer/probe systems based on each transgene target were used. The extracted DNA of each sample was...

Author(s): Xi Zhou, Hongwei Gao, Liqing Zhao, Wenda Liu, Min Sun and Shubai Wang

Low genetic diversity of Hypophthalmus marginatus from the Tocantins River based on cytochrome b sequence data

November 2013

  In this study, we used a portion of mitochondrial cytochrome b to evaluate the current genetic structure of the Hypophthalmus marginatus Valenciennes 1840, from the Tocantins River in the eastern Amazonia. Genetic diversity was measured in two downstream (Abaetetuba and Cametá), and two upstream (Tucuruí and Itupiranga) stocks under influence of the Tucuruí Hydroelectric Dam....

Author(s):   Emil José Hernández-Ruz, Evonnildo Costa Gonçalves, Artur Silva and Maria Paula Cruz Schneider

Multivariate analysis of some Ethiopian field pea (Pisum sativum L.) genotypes

November 2013

  The information on the nature and degree of diversity in the genotypes is crucial for efficient utilization of existing genetic resources. Thirteen (13) field pea genotypes along with two standard and one local checks were evaluated in triplicate randomized complete block design for three consecutive years to estimate the genetic variability and identify superior genotypes that generate putative transgressive...

Author(s): Seboka Habtamu and Fikreselassie Million

Role of apoptosis-related factors in follicular atresia

October 2013

Follicular atresia is the term used for the fate of follicles which undergo degenerative changes before rupturing during ovulation. Recent studies suggest that granulosa cell apoptosis play a major role in follicular atresia. The factors which lead the cell to apoptosis and which protect the cell death, still remain complicated and more studies are needed to elucidate the whole process. Here in this review, we aimed to...

Author(s): Basak Gumus, Sevtap Kilic and Ebru Zulfikaroglu

Effects of organic insecticides, Kingbo and Azdar 10 EC, on mitotic chromosomes in root tip cells of Allium cepa

October 2013

In this study, two organic insecticides (Kingbo and Azdar 10EC) extracted from plants and used as agricultural pesticides were investigated for cytotoxic and genotoxic effects using root tips of Allium cepa assay. Three different concentrations (0.625, 1.62 mL.L-1 and 2.5 mL.L-1) were used for different periods of time (8, 16 and 24 h). A single treatment of the effects of the two organic...

Author(s): Mona S. Al-Ahmadi

Inheritance of primary yield component traits of common beans (Phaseolus Vulgaris L.): Number of seeds per pod and 1000 seed weight in an 8 × 8 diallel cross population

September 2013

  Thirty six (36) genotypes (eight parents and 28 F1 diallel crosses) were grown in randomized complete block design with two replicates during 2006 at Mandura, North western Ethiopia. The experiment was executed to study the inheritance of two primary yield component traits: number of seeds per pod and 1000 seed weight. Statistical significant difference was observed between genotypes, parents and crosses for...

Author(s): Mulugeta Atnaf, Hussein Mohammed and Habtamu Zelleke

Molecular characterization of cotton using simple sequence repeat (SSR) markers and application of genetic analysis

September 2013

  Cotton is grown worldwide for the production of fiber and an important oil seed crop. Genetic diversity and correlation between varieties are of great importance for cotton breeding. To find out polymorphism and genome changeability, DNA markers are most comprehensively used. The current work was conducted to study the genetic relatedness among 20 cotton genotypes using simple sequence repeats markers using...

Author(s): Ambreen Ijaz, Sadia Ali, Usman Ijaz, Smiullah and Tayyaba Shaheen

Two genotypes of Xanthomonas oryzae pv. oryzae virulence identified in West Africa

August 2013

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is a very destructive rice disease worldwide. The aim of the present study was to examine if the Xoo virulence pathotypes obtained using phenotypic pathotyping could be confirmed using molecular approach. After screening of 60 Operon primers with genomic DNA of two Xoo isolates (virulent pathotype, Vr and mildly virulent pathotype, MVr), 12...

Author(s): Onasanya Amos, M. M. Ekperigin, A. Afolabi, R. O. Onasanya, Abiodun A. Ojo and I. Ingelbrecht

The genetic relatedness of drug resistant E.coli isolates of human and animal origin in Nigeria

August 2013

Molecular epidemiology of human and animal ecovariants of Escherichia coli from different regions of Nigeria were studied using their antibiotic susceptibility patterns, plasmid profile and pulsed-field gel electrophoresis (PFGE). E. coli was isolated using eosin methylene blue agar (EMB) and identified by conventional microbiological technique. The isolates were tested against 14 antibiotics using the disc diffusion...

Author(s): Chijioke A. Nsofor, Christian U. Iroegbu, Douglas R. Call and Margaret A. Davies

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