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Roselle (H.sabdariffa L.) is an economically important nutritional, medicinal, and industrial plant that belongs to the Malvaceae family and is native to Africa. In Ethiopia Roselle (H.sabdariffa L.) germplasm was introduced from different parts of Africa and maintained for breeding purposes in the Wondogenet agricultural research center, but there was no information on genetic diversity by using molecular markers. Thus,...
Cashew phyllosphere metagenomics reveals fungal phytopathogens of cashew trees from Kenya
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Cashew is an important cash crop in coastal Kenya. However, the emergence of phytopathogens poses a growing threat, leading to substantial losses that reduce both the quality and quantity of cashew products. The present study aimed to identify potential fungal pathogens associated with environmental samples from the cashew phyllosphere in coastal Kenya using an amplicon sequencing approach. The cashew samples were...
Isolation and Characterization of High Invertase-Producing Saccharomyces spp. from Palm Wine
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Palm wine, a traditional West African beverage, contains diverse microbial communities, including yeasts with valuable biotechnological potentials. This study investigated intracellular invertase production by Saccharomyces spp. isolated from palm wine samples collected across six locations in the Owo metropolitan area. Yeast cells were disrupted using three methods—mechanical grinding, sonication, and autolysis—to extract...
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Peste des petits ruminants virus (PPRV), the etiologic agent of Peste des petits ruminants (PPR), a highly contagious viral disease of small ruminants, remains a threat to small ruminant production in Nigeria. The objective of this study was to determine the prevalence of PPRV infection in tissues of apparently healthy sheep and goats slaughtered in Borno State, Nigeria. Three samples comprising lungs, lymph nodes, and...
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This study assessed the probiotic properties, antimicrobial bactericin and exopolysaccharide (EPS) production potential of lactic acid bacteria (LAB) isolated from metata ayib, a traditional Ethiopian fermented spiced cottage cheese, alongside their pathogen risks when inoculated into the product. Eighty LAB isolates were purified using MRS agar and identified through morphological, physiological, and 16S rRNA sequencing....
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Water quality is essential for human health, biodiversity, and sustainable development. Rivers, as major freshwater resources, are increasingly threatened by anthropogenic activities, affecting their physicochemical and microbiological properties. This study assesses River Ibi water quality across three sites (upstream, midstream, and downstream) during dry and rainy seasons. Standard analytical and microbiological...
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Tomato is a vital crop in Cameroon, yet local production heavily depends on costly imported hybrid seeds that are usually not adapted to local conditions. Developing high-performing local cultivars requires an understanding of the genetic diversity present in existing germplasm. This study aimed to evaluate the molecular diversity of tomato genotypes cultivated in Buea, Cameroon using simple sequence repeat (SSR) markers....
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Rhus tripartita, traditionally used to manage various diseases. However, many of these traditional uses remain scientifically unverified, especially for the species growing in Morocco. This study aims to address this gap by investigating some of the bioactive properties of methanolic extracts from the aerial parts and bark of Moroccan Rhus tripartita. The antioxidant activity was assessed using DPPH and ABTS assays....
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Cowpea is an important legume crop to farmers as a food, fodder, agronomic and income generating crop. Despite these multiple advantages, cowpea cultivation faces enormous abiotic and biotic constraints, including fungal diseases such as fusarium wilt, caused by Fusarium solani (Mart.) Sacc. One of the control measures of this disease, which causes severe yield losses, is the use of resistant varieties. However, the...
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In Ghana, viral diseases pose a major production challenge and are seen as a significant factor restricting the yield of vegetables. One of the viruses, the Cucumber Mosaic Virus (CMV), is highly destructive that correlates with yield losses from 30-50% and can infect a wider range of plant families compared to other plant viruses. A survey was therefore carried out from the month of June to October in the year 2022 to...
Xenobiotic Metabolism in the Aquatic Ecosystem: As a Biomarker for Environmental Pollution
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Xenobiotic substances with biological activities survive in the aquatic environment and are considered environmental contaminants. Detailed data analysis is required to understand xenobiotic interactions in aquatic ecosystems and their impacts in these ecosystems. Fish is known to be highly susceptible to xenobiotic interactions, and xenobiotic chemicals can cause either synergistic or antagonistic biological effects....
Drought Stress in Cassava: Management Strategies and Breeding Technologies - A Review
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Drought stress and water scarcity negatively affect cassava root development and yield production. The development, selection and deployment of cassava genotypes with enhanced drought tolerance and water use efficiency (WUE) will help to achieve food security in the face of climate change and a continuously growing population. The ability of cassava genotypes to produce optimum yields under limited water environments is...
Optimising the Mechanical Strength of Banana Pseudo-Stem Fibers (Musa Balbisiana)
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Lignocellulosic fibers are materials with a low resistance to shear forces during filtration due to their high hydrophobicity. Hence the aim of this study was designed to increase the fiber’s resistance to breaking forces. A composite design centered with four variables (soaking time, stirring speed, ratio of volume of solution to fiber mass and soda (NaoH) concentration) was used to optimize tensile strength using...
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Fish has been estimated to be 17% worldwide animal protein consumption. Fish and its products, nevertheless, have been established as a transporter of food borne diseases. This study was undertaken to determine the incidence, prevalence and antimicrobial sensitivity pattern of Salmonella enterica and Pseudomonas aeruginosa recovered from fish. Fifty (50) samples of fish were collected from Central Market, Gamji Gate and...
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Consumption of fresh khat (Catha edulis Forssk.) leaves has been shown to affect reproductive health, especially with regards to impaired male reproductive function. (-)-cathinone, the principal alkaloid in khat plant is known for its euphoric and psychostimulatory effects. Additionally, high dose and chronicity of exposure to khat extract and (-)-cathinone has been associated with impaired reproductive hormone synthesis...
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Dodonea viscosa is commonly used in traditional medicine because of its purported therapeutic benefits in the treatment of bacterial infections, cancer and inflammation among others. The present study aims to evaluate the antibacterial, antioxidant and anticancer effects of the leaf and stem extracts of Dodonea viscosa. The antibacterial properties of the extracts of D. viscosa leaves and stem were evaluated using disc...
Development of Paper-based colorimetric strip for rapid detection of Phytochemical
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One fascinating discipline in Biotechnological studies is the examination of phytochemicals as possible agents for developing medications and enhancing nutritional characteristics. The phytochemicals have impacts for many areas, like pharmaceutical development, food quality control, and studying plants. The newly suggested paper-based colorimetric method was used coupled with traditional procedures to perform chemical...
Monoclonal Antibodies Production Through Hybridoma Technology: A review
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Laboratory-made molecules known as monoclonal antibodies are designed to act as replacement antibodies and repair, improve, alter, or imitate the immune system's attack against unwanted cells. These antibodies identify specific binding sites, or epitopes, on a particular antigen. The protein sequence, antigen-binding site area, binding affinity for their targets, and downstream functional effects of mAbs are all similar....
Bacillus thuringiensis: A Nature Package for Agriculture, Health and Industrial Application
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Bacillus thuringiensis (Bt) is a naturally occurring sporulating bacterium isolated worldwide from different ecosystems. It is packed with several Cry proteins that are toxic to different organisms. The presence of Cry toxins is the main factor conferring entomopathogenic properties to Bt which makes Bt different from other Bacilli. The Cry proteins are encoded by Cry genes, there are many Cry genes. Some single strains...
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Neem tree (Azadirachta indica A. juss) products, including seed, possess lots of scientific and economic benefits. Neem seeds contain adequate amount of azadirachtin, a triterpeniod compound, which can also be obtained from other parts of the plant. The compound acts as an insect repellent by inhibiting them from feeding and disrupting their increase reproduction. However, there may be sparse literature on secure human...
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Introduction: Malaria is one of the most prevalent public health problems worldwide. Globally, nearly half the world’s population remains at risk of malaria transmitted by Anopheles mosquito vector. Chemical insecticides, which are toxic and cause environmental deprivation, have been used to control the vector. So,safe environmentally friendly and effective alternative method are need to control malaria vectors. ...
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Escherichia coli (E. coli) and Salmonella enterica (S. enterica) are Gram-negative bacteria commonly found in the gastrointestinal tract of animals and can cause illness in humans, particularly through contaminated meat products and their handling. Genetic identification techniques offer rapid and precise methods for characterizing these bacteria and revealing their genetic diversity. This study aimed to investigate the...
A Relationship between Soil bacterial Community and Ginseng Rusty Root Disease
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Ginseng rusty root disease (GRRD) is one of the major diseases of ginseng, which disease is leading to a severe decline in the quality and yield of ginseng. Soil bacterial communities’ long-term survival in soil, and it plays an important role in the growth of ginseng health. To clarify the relationship between soil bacterial community and GRRD, soil properties, bacterial community structure and diversity from eight sites...
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In Ikwoka, Southeast Nigeria Hemisus guttatus and Milvus migrans parasitus are good comparatively bio indicators due to non migration and occupation of different terrestrial environments during seasonal migrations respectively. Hemisus guttatus is rare while Milvus migrans parasitus is seasonally frequent. This study is to establish possible cause of Hemisus guttatus rare status between parasites and other environmental...
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Sesame (Sesamum indicum L.) is a significant oilseed crop that is cultivated in various regions across the globe. Its seed is well known for its high oil content (50-60%), thus earning it the title of the supreme oilseed. Nigeria, being among the top 10 largest producers of sesame, possesses immense potential to enhance production for the purpose of promoting exports. Sesame exhibits extensive genetic diversity, which can...
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A study was conducted to determine the genetic variability and patterns of diversity of finger millet (Eleusine coracana) accessions using six ISSR molecular markers. The study consisted of 72 finger millet accessions with variable seed coat colors collected from two zones of the Tigray region. The markers generated 45 loci, 30 of which were polymorphic and 15 of which were monomorphic, for an average of 7.5 loci per...
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In my research, the results of the physical-chemical analysis and sensory analysis of wine production from local and otheri valuable technical grape varieties cultivated in the diverse and rich soil-climatic conditions of our country and the produced wines were reflected. Samples were taken from the produced products, the amount of sugar content, acidity and other important indicators were determined by modern methods and...
Brewing 4.0: Unleashing the Power of Industrial Internet of Things for Smart and Sustainable Brewing
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The rapid evolution of the Internet of Things (IoT) has given rise to the Industrial Internet of Things (IIoT) otherwise known as the 4th Industrial Revolution. Embracing IIoT in the brewing industry, also known as Brewing 4.0, promises revolutionary advancements in brewery operations. Breweries like Ab InBev and Sugar Creek are advanced levels of implementation of IIoT. The implementation of which has decreased process...
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Septoria leaf blotch is one of the most devastating diseases of wheat worldwide. Understanding its genetic diversity and population structure is paramount importance in designing and sustainable management strategies to control the disease. Therefore, the present study was designed to detect and determine the genetic diversity and population structure of Z. tritici isolates collected from northern Ethiopia: Amhara and...
The Antioxidant, Protective, and Nutritional Roles of Folic Acid “Vitamin M”
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Folic acid is a water-soluble vitamin that comes after the vitamin B complex and is one of the compounds that have antioxidant activity and protective against some diseases caused by harmful oxidation. Recently, through studies and scientific research conducted on folic acid, the extreme importance of this vitamin has emerged after cases of deformity occurred in children due to a lack of nutrition from sources containing...
NEEM (OIL) AND CROP DEFENCE: (A desire for the future)
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The mission of agriculture is to improve food production to satisfy the needs of the developing global populace, without damaging the surroundings. In Present day agricultural practices, the control of pests is regularly performed via the excessive use of agrochemicals that can bring about environmental pollutants and the development of resistant pests. In this context, biopesticides can offer a better opportunity for...
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Auricularia species is a jelly- like edible fungus usually with mild flavour. It is also known as wood ear mushroom because of its ear-shaped morphology. Nigeria is blessed with many species of these edible mushrooms; however, not much research effort has been given to the cultivation of Auricularia species. Wild species of Auricularia were collected from different locations within southern Nigeria. Pure cultures of these...
ADSORPTION OF CHROMIUM (VI) AND LEAD (II) IN SYNTHETIC SOLUTIONS USING TAMARINDUS INDICA FRUIT PEEL.
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Adsorption allows minimizing the generation of toxic waste and the recovery of the metal. The objective of the work was to study the bioadsorption of Cr (VI) and Pb (II) using the dry peel of Tamarindus indica. We worked at different pH values and concentration levels. The determination of the chemical-physical parameters was carried out at the Empress Geominera Oriente. Adsorption isotherms were performed using the...
AI-Metaverse Bioinformatics: Potential future direction
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Bioinformatics has been around since the 1960s, while online molecular databases that handle data generated by disciplines in the life sciences have existed since the 1990s. This Perspective presents a forward-looking approach that amalgamates bioinformatics with new and potential fields such as generative artificial intelligence and metaverse that can catapult bioinformatics into user-friendly 3D virtual worlds with...
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The specific objectives of this research were (i) to develop broad-scale bioinformatics data from three lactic acid bacteria (LAB) strains - S. thermophilus KLDS 3.1003, SMQ-301, and LMD-9 genomes, (ii) to mine the relevant genes that confer probiotic and industrial properties to the strains, and (iii) show a more precise correlation between reported in vitro and in vivo properties of these strains and their genome...
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Meat is highly nutritious, being a good source of various nutrients especially protein, fat and vitamins. Its composition is ideal for the growth of a wide array of spoilage bacteria. Food-borne infections outbreaks affect both developing and developed nations. The infections caused by emerging pathogens like Enterohaemorrhagic E. coli 0157, Listeria monocytogenes, Vibrio vulnificus, Campylobacter specis and...
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A large part of transcripts is non-coding, which is transcribed from junk DNA; long non-coding RNAs and micro-RNAs regulate the expression levels of mRNAs. For the first time, we identified lncRNAs and miRNAs with their regulatory role in the disease tolerance of Theobroma cacao. In this study, about 2616 lncRNAs and 153 miRNAs were identified from 10 RNA-seq data representing healthy and witches’ broom diseased tissues of...
Evaluation of Artificial Insemination Delivery Services to Smallholder Dairy Farmers in Ethiopia
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The study was carried out in dairy production potential areas of Oromiya, Amhara and Sothern Nations Nationalities People’s Region (SNNPR) with the objectives of evaluating artificial insemination delivery service on small holder dairy farmers, identify major constraints of artificial insemination delivery service and identify ways of alleviating problems related to artificial insemination delivery service. The study...
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Hexavalent Cr is most toxic metal and hazardous compound worldwide. It is also considered to be cellular oxidizing pollutant causing damage, mutagenic and hematopoietic effects to living systems. Present work is focused on synthesis of zero valent iron nano particles (Fe-NPs) using Bacillus subtilis SHB 13, Achromobacter xylosoxidans SHB 204 and Surfactin and their application for Cr (VI) detoxification (100 ppm) in...
Quantitative Phytochemicals and Antioxidant Potential of Adansonia digitata leaves and stem extract
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The objective of this study was to examine the phytochemical properties of the baobab tree (Adansonia digitata) stems and leaves. The dried powder of leaves and stem extract were analyzed for the presence or absence of different phytochemicals, flavonoids, terpenoids, saponins, tannins, alkaloids, etc. The quantitative analysis revealed that the leaves and stem of A. digitata are rich in total phenols, alkaloids,...
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Dekoko is a marginalized, but economically important and endemic cool - season legume crop in Ethiopia. However, the molecular characterization of this crop has not been carried out regardless of its importance as source of protein for the rural poor in Ethiopia. This study is therefore aimed to investigate the genetic diversity and population structure of 83 Dekoko accessions collected from north Ethiopia. Three plants...
An overview of proteolytic enzymes and determination of protein content in the proteinous sample
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The world environment is changing by the socio economic factors. Most of this occurs by using chemical in industries. Digestive enzymes plays a great role in breakdown of macromolecules into micro molecules Protease also called proteinase or peptidase is a digestive enzyme that categorized under proteolytic enzymes and replaces these chemicals in industry. Protease categorized into different classes based on different...
Fermented Bamboo Shoot Health Benefits and Applications
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The term "bamboo" conjures up images of "a hollow stick," and it aids more health benefits. Mainly the tribes of northeast India ferment these nutritious bamboo shoots. Fermentation is a biotechnological process that has been used to preserve food for centuries. Fermented bamboo shoots caters a home and benefits for a variety of microorganisms. To highlight a few benefits these magical shoots are probiotic, anti-oxidant,...
Phytochemical, antioxidant and anti-proliferative activity of Epipremnum aureum
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Objective: The study was primarily carried out to assess the phytochemical contents present in the leaf of E. aureum. Herein, biochemical characterization vis-à-vis antioxidant potential and antiproliferative activity on cancer cell lines is experimented too. Methods: Healthy leaves of E. aureum after authentication were evaluated in vitro for phytochemical, antioxidant and antiproliferative activity. Various non-enzymatic...
Comparative physiological studies of parental and fusant protoplasts culture of Volvariella volvacea
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Very little information is available in the physiology, biochemistry and molecular biology of Volvariella volvacea. The current study was carried out to understand the effect of different macro- and microelements and vitamins on the mycelial growth and yield of protein by the mycelia of parental and fusant protoplasts strain of V. volvacea. The method adopted in this experiment was to study the additive and deficient...
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Application of DNA fingerprint is the method which permits documentation of genetic material with high accuracy and permanency as it is not predisposed by environment, epistatic interactions and other factors. Seed quality and purity are the important key aspects to be maintained in seed multiplication and production to enhance yield and productivity. The aim of this study was to assess genetic purity of yellow bean...
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Background: Hypertension is a common debilitating illness among people in both developed and developing countries. This study investigated the effect and possible mechanism of the antihypertensive activity of lycopene-rich extract of Solanum lycopersicon (LRESL) on Wistar rats. Methods: Sixty (60) hypertensive Wistar rats were divided into seven experimental groups viz: Group A served as a normotensive group and received...
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Lignocellulosic biomass is applauded to be the cheapest resource for second generation biofuels. However, the degradation of lignocellulosic biomass is quiet challenging due to the complexity in structure. The study investigated the saccharification potential of in-house enzymes produced from a white-rot basidiomycete strain, Polyporus ciliatus CBS 366.74 on a lignocellulosic biomass such as pretreated corn stover. The...
Genetic characterisation of Colophospermum mopane and its ecotypes using RAPD analysis
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Colophospermum mopane and its ecotypes are currently recognized on morphological and physiological characters. To add to the suite of taxonomic characters, the genetic variability of C. mopane (sensu stricto) and its ecotypes were investigated using Random Amplified Polymorphic DNA. DNA was extracted from young and mature leaves using the CTAB method. Twenty random primers were tested for their suitability and...
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AFLP markers were used for evaluating genetic diversity, population substructure, species relationships, and gene flow among eighty-five accessions belonging to the mungo-radiata group of section Ceratotropis of subgenus Ceratotropis of genus Vigna. Twelve preselected AFLP primers generated 1869 polymorphic amplification products. The number of fragments for each primer pair ranged from 44 to 296, showing 100%...
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