African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Responses of commercial chickpea (Cicer arietinum L.) cultivars to varying moisture stress conditions under rain-fed production system in Ethiopia

Article in Press

Chickpea is an economically important crop grown by nearly one million Ethiopian smallholder farmers. The crop is often considered as “stress-loving”, but moisture stress at critical growth stages could be detrimental. Yield of chickpea is commonly affected by terminal drought stress in the rainfed production system in Ethiopia. The lack of proper field-screening methods has hindered the development of drought tolerant...

Author(s):Lijalem Korbu, Asnake Fikre, Kassahun Tesfaye, Assefa Funga, Dagnachew Bekele and Chris O. Ojiewo

Diversity of arbuscular mycorrhizal fungi in the three agroecological zones of the Central African Republic

Article in Press

Arbuscular mycorrhizal fungi (AMF) are organisms that can establish symbioses with 80% of terrestrial plants. Soils were collected in three agroecological zones (AEZ) of the Central African Republic (CAR). The aim of this research is to study the diversity of AMF in the CAR. The spore extraction and enumeration, granulometric and physicochemical analyses of each soil were performed. The results showed that AEZ3 has a large...

Author(s):Mathurin DJAMNDO DJASBE, , Hubert Dieu Béni ELIAN , Tahir Abdoulaye Diop, Denis Omokolo Ndoumou

Chitin deacetylase of Bemisia tabaci (Hemiptera: Aleyrodidae) as new target for pesticide discovery: An in silico investigation and hints for insecticide discovery

Article in Press

Chitin deacetylases are enzymes responsible for structuring chitin of chitin-producing organisms. Chitin deacetylases are well studied in fungi and bacteria, but the canonical structure was just recently characterized for an insect, the silk moth Bombyx mori (Lepidoptera: Bombycidae) (PDB: 5ZNT; 5ZNS). The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a polyphagous insect that causes severe loses in agriculture every...

Author(s):Ciro Pedro Guidotti Pinto

Effect of intercropping aerobic rice with leafy vegetables on crop growth, yield and its economic efficiency

Article in Press

Aerobic rice production system provides a sustainable alternative to the traditional rice cultivation. To evaluate the agronomic and economic effect of intercropping aerobic rice with four leafy vegetables, field experiments were conducted at University of Agriculture Science Bengalore research station, India during 2017 and 2018. The experiments consisted of 9 treatments with 4 replications and, a Randomized Completely...

Author(s):Sylvestre Habimana ,Saidi Rumanzi MbarakaAntoine Karangwa, Francois Xavier Rucamumihigo, Fabrice Rwalinda Musana, Eularie Mutamuliza, Ivan Gasangwa, Christian Nkurunziza, Jean Claude Abayisenga, Athanase Rusanganwa Cyamweshi

Improving the viral resistance in potato by classical and molecular breeding methods: A review

Article in Press

Potato is a versatile crop, and it has owned one of the richest gene pools of any other cultivated plants. However, numerous biotic agents influence the crop, resulting in variable degrees of yield loss in both quantity and quality for growers. There are more than 40 different virus diseases infecting potatoes worldwide. The most detrimental major potato viruses are potato virus Y, potato leafroll virus, potato virus A,...

Author(s):Molla Gererme Taye, Taller Janos, Istvan Cernak, and Gyula Vida,

The Determination of Chemical Composition in Some Vinegars and Compliance with Food Legislation

Article in Press

This paper covers that the six different brand sold (genuine-organic-synthetic apples and grapes) in Turkey and spontaneous fermentation vinegars produced with seven fruits (apple, white and red grapes, mandarin, pear, carrot, melon) in the laboratory had been analysed according to pH value, acidity, density, the amount of dry matter, color measurement, the ash content and the water activity. The aim of the study was to...

Author(s):Ahmet Dogan Duman, Alaeddin Türkmen

TrichoPlus (Trichoderma asperellum) as a promoter of vegetative growth in soybeans

Article in Press

Fungi of the genus Trichoderma are found naturally in the soil and used as an active ingredient in biofungicides and also have activity as promoters of plant growth. This work aimed to evaluate the efficiency of the product TrichoPlus, containing Trichoderma asperellum as a promoter of soybean plant growth. Four independent experiments were carried out, in different periods between 2019 and 2020. The experiments were...

Author(s):Aloisio F. Chagas Junior, Lillian F. B. Chagas, Brigitte S. O. Colonia, Magno R. Carvalho Filho; Luciane O. Miller

The reconstruction of metabolic pathways in selected bacterial and yeast strains for the production of bio-ethylene from crude glycerol: A mini review

Article in Press

The role of bioengineering in industrial processes cannot be ignored, especially in circumstances where the processes are uneconomical, demand high energy, use finite resources and emit huge amounts of carbon dioxide. Ethylene is a two-carbon unsaturated hydrocarbon that is industry's most important building block for polyester fibres, plastics, and ethylene glycol. For decades, ethylene production has relied on...

Author(s):Idan Chiyanzu

GENETIC VARIABILITY ANALYSIS OF ELITE SORGHUM (Sorghum bicolor (L) Moench) GENOTYPES UNDER DRY LOWLAND AREAS OF ETHIOPIA

Article in Press

Narrow genetic base, information on the genetic variation, early maturing and drought tolerant sorghum genotypes are the most important limiting factors for sorghum yield improvement under moisture stress areas. Therefore, the experiment was conducted to quantify the genetic variation available among sorghum genotypes for drought tolerance related traits. A total of 42 sorghum genotypes were evaluated using alpha lattice...

Author(s):Temesgen Begna

IN-VITRO PROPAGATION OF VCF 0517 SUGARCANE VARIETY USING MERISTEM CULTURE.

Article in Press

VCF 0517 is newly released sugarcane variety in India which is high yielding, high tillering, better quality, suitable for wide row planting, high jaggery yield and quality and good rationing ability. The objective of this study was to evaluate conditions required for the in vitro propagation of VCF 0517 sugarcane genotype. Callus regeneration was done using meristem explant on MS + 2,4-D (0-5mgL-1) media to determine most...

Author(s):BRIAN SSEMUGENZE, YUSUF ABBAS, NAGESHA, N.

A SURVEY OF GENETICALLY MODIFIED FOODS IN NAMIBIA SUPERMARKETS

Article in Press

The present study was carried out to survey genetically modified organisms (GMOs) in foods and feeds found in local supermarkets in Namibia. Little is known of the presence and prevalence of GMOs in the local supermarkets or information available on the labelling provided on some. The aim was to verify the presence of GMO’s in the foods and feeds using molecular extraction and screening methodologies. Nine samples (5...

Author(s):Faith Fransisca Kavishe, Percy Chimwamurombe and Jeya Kennedy

Molecular identification of fungi causing canker and dieback diseases on Vangueria infausta (Burch) subsp. rotundata (Robyns) and Berchemia discolor (Klotzsch) Hemsl in lower Eastern Kenya

Article in Press

Vangueria infausta (Burch) subsp. rotundata (Robyns) and Berchemia discolor (Klotzsch) Hemsl are drought tolerant multipurpose fruit trees indigenous to the lower Eastern Kenya. These fruit tree species are candidates for dryland agroforestry and could provide food, medicine and other commodities required by the local communities. Surveys conducted during this study showed that they suffer from dieback and canker. This...

Author(s):Susan Karani, Jane Njuguna , Alice Muchugi, , Steven Runo and Joseph Machua

Distribution of Mutations in G-6-PD Gene. A Review.

Article in Press

Exploring molecular techniques to unwind possible mutations in the exons, introns and promoter regions of glucose-6-phosphate dehydrogenase (G-6-PD) gene is indispensable. Such information would improve neonatal health and haemolytic diseases including malaria control especially in malaria-endemic areas as the severity of these diseases is associated with the type of molecular variants. G-6-PD deficiency literature was...

Author(s):Jelani I, Alhassan AJ and Abdullahi HL

Characterization and potential applications of Bromelain extracted from pineapple (Ananas comosus) juice processing waste (peel) for recovery of Silver from used X-ray films

Article in Press

Bromelain is a proteolytic enzyme, which is prominently found in all parts of a pineapple plant (Ananas comosus). It has found immense applications in the pharmaceutical industry as well as the food, cosmetic, and leather industries. However, bromelain from the pineapple fruit peels is a less explored source for making valuable products. Therefore, the objective of this study was to investigate the potential application of...

Author(s):Muhammed Seid Anbesaw

Anticancer and Antimicrobial Properties of Porous Starch Mediated Silver Nanoparticles

Article in Press

Health issues involving inadequate treatment of diseases like cancer and microbial infections continue to be the subject of much ongoing recent research. Biosynthesized Silver Nanoparticles (AgNPs) were characterized using Transmission Electron Microscopy (TEM), Zeta Sizer, Ultra-violet (UV), and Fourier Transform Infrared (FTIR) Spectroscopy. Antimicrobial activity was evaluated in selected gram-positive and...

Author(s):Shiara Ramdath, John Jason Mellem and Londiwe Simphiwe Mbatha

OPTIMIZATION OF CALLUS INDUCTION FOR WHOLE PLANT REGENERATION IN RICE (Oryza sativa L.) CULTIVAR NERICA-4

Article in Press

The interspecific rice hybrid of the Asian Oryza sativa and the African Oryza glaberrima, New Rice for Africa (NERICA-4) development was initiated due to threatening global food insecurities in part due to its desirable agronomical features compared to other NERICA cultivars. It is fairly tolerant to drought, has quicker growth rate and high yields. However, production is still faced by numerous challenges, leading to...

Author(s):Patroba O. Odeny, Joyce M. Luganji, Joel Masanga, Godfrey M. Ngure, Steve M. Runo

Meiotic Chromosomes Behavious in Chlorophytum stenopetalum Bak

Article in Press

Chromosomes behavior at meiosis in Chlorophytum stenopetalum Bak. was investigated especially at pachytene /diakinesis and metaphase. The flower buds at right age were harvested, fixed in Cornoy’s solution (3 part of absolute alcohol and 1 part of acetic alcohol) and preserved in a refrigerator at -4°C for at least thirty minutes. The flower buds were then hydrolyzed in 10% HCl for 3-5 minutes. Prepared slides were...

Author(s):Omokanye Babatunde Sikiru

REGULATION OF SALT AND DROUGHT STRESS RESPONSES BY SDR IN ARABIDOPSIS AND IDENTIFICATION OF PROTEINS INTERACTING WITH SDR

Article in Press

The ubiquitin/26S proteasome pathway is key to protein degradation in plants. Its specificity often orchestrated by ubiquitin-protein ligases (or E3s), which facilitate the translocation of ubiquitin to appropriate targets. F-box protein is one of the subunit of E3 ligases SCF (Skp1-Cullin/CDC53-F-box). It has been reported that F-box protein is not only related to plant growth but also abiotic stress. In this study, the...

Author(s):Justus Obara, Wilfred Abincha

HEAVY METALS AND VITAMIN CONTENT OF “KUNUN-ZAKI” DRINK, PULP AND THEIR FREEZE-DRIED FORMS

Article in Press

Significant adverse health effects of certain heavy metals were reported in the dried samples of food. This study was aimed at evaluating vitamins, and heavy metals content of unsweetened/sweetened “kunun-zaki” drink/pulp, before/after lyophilizing. The material tested were unsweetened and sweetened “kunun-zaki” drink- UKD, and its pulp- UKP, sweetened “kunun-zaki” drink- SKD, and its pulp- SKP before and after...

Author(s):Esienanwan Esien Efiong, Emmanuel Hala Kwon-Ndung, Abraham Ehinomhen Ubhenin, Jonathan Maduka Nwaedozie

Contact activities Piper guineense(Schum and Thonn) And Eugenia aromaticum(L).(Merril and Perryl) Extracts against Larvae of Hide Beetles, Dermestes maculatus(Degger) (Coleoptera: Dermestidae)

Article in Press

Contact activities of Piper guineense(Schum and Thonn) and Eugenia aromaticum( L). (Merril and Perryl) extracts in the control of Dermestes maculatus larvae infesting stored fish (Clarias gariepinus) was investigated under laboratory condition (28 ±3oC and 65± 5% R,H.). The extracts were tested by application of 2.0µL each to ten third instars larvae using micro pipette at a concentration of 6.00, 10.00 and...

Author(s):Jatau, A., Yahaya, M. A., Majeed, Q. And Sokoto, M. B.

Using marker assisted backcrossing procedure to introgress Co-42 and Co-5 anthracnose resistance genes from G2333 into a susceptible commercial bush bean variety in Uganda

Article in Press

Anthracnose caused by Colletotrichum lindemuthianum is an important disease of common bean worldwide. In Uganda, two commercial bush bean varieties NABE13 and NABE14 with acceptable red seed types are highly susceptible. Our objective was to transfer the Co-42 and Co-5 anthracnose resistance genes from donor parent G2333 to NABE14 using a backcrossing program aided by molecular markers. The SH18 and SAB3 markers were...

Author(s):Annet Namayanja, Annet Namusoke, Jane Mukabaranga, Nampijja Shakirah and Paul Gepts

Effect of Growth Regulator Concentrations on in vitro Multiplication of three Elite Sugarcane (Saccharum officinarum L.) genotypes using Shoot tip Culture

Article in Press

Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. This results in shortage of quality planting materials. Micropropagation is currently the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely...

Author(s):Gerema Amente, Tileye Feyissa

Quality Evaluation of Low Free Fatty Acid and High Free Fatty Acid Crude Palm Oil and Variation of Total Fatty Matter and Fatty Acid composition in Nigerian Palm Oil

Article in Press

The quality of Palm oil determines its consumer and market acceptability and price, and this correlates directly to its Moisture (%), Free Fatty Acid (% FFA) and Total Fatty Matter (%TFM) content. Therefore, businesses utilizing palm oil for production are very particular about these quality parameters. The aim of the present study was to evaluate the quality of Low free fatty acid (LFFA) and high free fatty acid (HFFA)...

Author(s):IGILE GO, IWARA AI, EKPE OO, ESSIEN NM, EGBUNG GE

Antimalarial potential and protective properties of Azadirachta indica (Dongoyaro) ethanolic stem bark extract in mice infected with Plasmodium berghei NK65

Article in Press

The study determine the in vivo antiplasmodial potential and protective properties of Azadirachta indica (Dongoyaro) ethanolic stem bark extract in mice infected with Plasmodium berghei NK65. The GC-MS, qualitative, quantitative and antioxidant scavenging activities of the stem bark extract of A. indica were determined using standard procedure. The acute toxicity and in vivo antiplasmodial activities of the extract were...

Author(s):Momoh Johnson Oshiobugie, Adeniyi Michael Olaniyi and Aderele Oluwaseun Raphael

New Elite Sugarcane Varieties, 'Tafach Shenkora' and 'Kay Shenkora' , for Commercial Production in Ethiopia

Article in Press

'Tafach Shenkora' and 'Kay Shenkora' sugarcane (Saccharum spp.) are the names assigned to sugarcane varieties with accession numbers Acc. 43 and Acc. 139, respectively. They were developed by Sugar Corporation, Research and Development and were registered and released in June 2019 by the National Variety Releasing Committee of Ethiopia to growers in three sugar estates in Ethiopia. 'Tafach Shenkora' and 'Kay...

Author(s):Esayas Tena, Firew Mekbib, Abebech Seife, Zinaw Dilnesaw and Feyissa Tadesse

Molecular Characterization, Optimization of Polyhydroxybutyrate (PHB) production using agro-wastes as substrate by Bacillus cereus

Article in Press

Non biodegradability of plastics has discouraged its use and hence alternative eco friendly polyhydroxybutyrates have gained popularity. The use of cost effective and easily available substrates in the production of polyhydroxy butyrate (PHB) enhances its application and further reduces its cost and hence PHB can be efficiently used instead of plastics. The present work was performed to study the optimum production of...

Author(s):Jasmine R, Hannah S

Isolation and characterization of lactobacilli in raw milk derived whey and its potential use as probiotic

Article in Press

The main purpose of this study was to isolate lactobacillus from raw milk derived whey and to determine its potential use as probiotics. Fifteen samples of milk were obtained and tagged (LS1 to LS15) from a homogeneous group of Holstein cows and later at our laboratory the milk was processed to obtain whey, which was inoculated in MRS medium where we got 30 bacterial isolates and selected those who were bacilli gram...

Author(s):Guel-García G. Paulina, Rodríguez Castillejos Guadalupe C., Hernández Mendoza José L.

The adults of Anthonomus grandis as disseminator of Fusarium chlamydosporum and use of oregano extracts for their control

Article in Press

The Cotton Weevil is an insect that causes considerable losses to the cotton industry. Its larvae grow inside the cotton bolls, damaging them. It enters diapause during the winter and becomes active again in the first months of the year. Pheromone traps can be used to estimate their populations and implement control measures. This work analyzes samples of weevil populations collected in traps placed on mexican...

Author(s):Jesus DiCarlo Quiroz Velasquez; J. Luis Hernandez-Mendoza, Guadalupe Rodriguez Castillejos; Gerardo Sanchez Ramos; Cristian Lizarazo Ortega; Jesus Gerardo Garcia Olivares

GENETIC DIVERSITY OF PROLACTIN GENE IN JAPANESE QUAIL (Coturnix coturnix japonica)

Article in Press

This study was carried out to investigate the genetic diversity of prolactin gene (PRL gene) in Japanese quails using PCR-RFLP marker. One hundred and thirty nine quails were sampled for PRL loci analysis. DNA was extracted from the samples. Polymerase chain reaction (PCR) and electrophoresis was used to characterize a 24 base pair (bp) insertion/deletion in a 358 bp PCR product. Test for Hardy-Weinberg Equilibrium were...

Author(s):FRANCISCA OSEAHUMHEN EICHIE, ADEBOWALE EMMANUEL SALAKO, HENRY OSAMEDE OSAIYUWU

Phytoremediation: A general Review

Article in Press

This paper makes a general review of phytoremediation emphasising a bit on phytoextraction, in terms of factors that affect it and possible enhancements of such factors for optimization. Phytoremediation is a diverse collection of plant-based technologies that are used to clean contaminants such as heavy metals and metalloids (HMs) from environments, especially soil and water bodies. HMs are toxic to most biota, but some...

Author(s):TAOLO BONNIE TUMEDISO, B MOSEKI

Antifouling Activity of Amphiora anceps on Marine Diatoms: a Comparison with Copper Sulphate

Article in Press

The search for environment-friendly and non-toxic antifouling paint components has led to the investigation of natural products from seaweeds. The defence metabolites utilized by seaweed to deter redundant epibiosis have potential for harnessing and use in antifouling applications. A search for new non toxic antifouling paints has been tested in collected seaweed such as Amphiora anceps. The seaweed was extracted with...

Author(s):Murugan Suresh, Palanisamy Iyapparaj, Perumal Anantharaman

REVEALING EXPRESSION OF R GENES HOMOLOGUES AGAINST CLCUV IN GOSSYPIUM ARBOREUM AND G. HIRSUTUM COTTON GENOTYPES

Article in Press

Cotton leaf curl virus (CLCuV) is a major threat, limiting the fiber yield in Gossypium hirsutum. Resistance (R) genes are present in G. arboreum against this virus but these R genes are yet not known. Some resistance gene analogs (RGAs) of nucleotide binding site leucine rich repeats (NBS-LRR) have been reported in G. hirsutum. In the present study, RGAs from G. hirsutum and ESTs predicted as cell surface receptor genes...

Author(s):RAKHSHANDA MUSHTAQ, Muhammad Asif, Aftab Bashir

CANE YIELD AND JUICE VOLUME DETERMINES ETHANOL YIELD IN SWEET SORGHUM (Sorghum bicolor L. Moench)

Article in Press

Sweet sorghum (Sorghum bicolor L. Moench) contains fermentable sugars in the stem that can be converted to ethanol. The current study aimed at evaluating the performance of three sweet sorghum genotypes with five checks and contributes towards availing suitable sweet sorghum for industrial ethanol production. Field studies were carried out in Kenya at varied locations in a randomized complete block design with three...

Author(s):

Stabilization of corn oil by phenolic camellia oil cake extract during storage

Article in Press

Camellia oil cake extracts was used as a source of phenolic compounds. Isopropanol, normal hexane and diethyl ether were evaluated as different solvents to extract these phenolic compounds which examined by Folin-Ciocalteu assay. The antioxidant potential of camellia oil cake extracts was measured by ferric reducing antioxidant power assay and results showed that the antioxidant activity strongly depend on the solvent....

Author(s):FEI SONG, Hui WANG

Efficacy of botanical pesticides against Acanthoscelides obtectus (Coleoptera: Chrysomelidae): A strategy for insect pest control

Article in Press

Laboratory experiments were carried out to investigate the efficacy of botanical insecticidal materials against a major insect pest of stored beans, Acanthoscelides obtectus. The materials employed by small scale farmers to reduce the problem of bean postharvest losses caused by A. obtectus were identified and collected from Dar es Salaam and also from Kimuli and Mabira villages in Kagera region in Tanzania. The materials...

Author(s):Rugumamu Costancia Peter

Genetic improvement for green pod yield and number of pods in two segregation populations of pea (Pisum sativum L.)

Article in Press

This study was conducted at the Experimental Farm of The Faculty of Agriculture, South Valley University, Qena Governorate during three successive winter seasons of 2011/2012, 2012/2013 and 2013/2014 to improve green pod yield/plant (g) of field pea. The breeding materials used in this study were F3, F4 and F5 generations of the two field pea crosses Alaska X Little Marvel (population 1) and Alaska X Dwarf Gray sugar...

Author(s):Rashwan A., M., A

Hypolipidemic, Hypoglycemic and Anti-obesity effects of Opuntia ficus indica aqueous extract in rabbits

Article in Press

Opuntia ficus indica is used in traditional medicine for the treatment of hyperlipidemia, hypercholesterolemia, diabetes and dermal burns. The aim of this study was to analyze and compare the effects caused by the Opuntia ficus indica aqueous extract on serum and lipid parameters in white male rabbits (initial mean weight 1.95 kg). Rabbits were divided into two groups (n=6), the first group represented the control group,...

Author(s):HALMI S

COMPARATIVE STUDIES ON PRODUCTION OF BIOETHANOL FROM PEELS OF COCOYAM AND SWEET POTATO USING Aspergillus niger AND Saccharomyces cerevisiae

Article in Press

The aim of this research is to compare the potential of sweet potato and cocoyam peels (non-edible waste material) for bioethanol production using sequential monocultures of Aspergillus niger and Saccharomyces cerevisiae and co-cultures of Aspergillus niger and Saccharomyces cerevisiae. Each sample was dried and ground; and was subjected to heat pretreatment. Direct fermentation of pretreated sweet potato and cocoyam peels...

Author(s):DANIEL UDENZE and Victoria Adegunloye

USE OF MARKER ASSISTED SELECTION (MAS) FOR THE CHARACERIZATION AND INTROGRESSION OF RUST RESISTANCE GENES USING AN F2 ROBIN/KWALE POPULATION

Article in Press

Breeding for durably resistant varieties is amongst the best strategies to exterminate the threat posed by stem rust race Ug99 of wheat. This race has continued to spread and ascertain itself as a danger to global wheat production and consequently food security. The continuous swift development of new DNA marker technologies has proved invaluable in breeding for durable rust resistance to stem rust race Ug99 and its...

Author(s):BERNICE NGINA WAWERU, PETER NJOROGE NJAU, GODWIN KAMAU MACHARIA, OLIVER KIPLAGAT

A COMPARATIVE STUDY ON THE PROXIMATE AND PHYTOCHEMICAL ANALYSIS OF THE MALE AND FEMALE PARTS OF CARICA PAPAYA

Article in Press

The phytochemical analysis of the male and female plant parts (leaves, flower and bark) of Carica papaya was investigated. The analysis revealed the presence of some plant bioactive components. There was a difference in the bio-active component of the male and female leaves, both leaves revealed the presence of alkaloid, tannin, phlobotanins, saponin, flavonoid, anthraquinones, phenol and cardiac glycosides. Terpenes,...

Author(s):DEBORAH ONIFADE

Lactic acid bacteria fermentation of coconut milk and its effect on the nutritional, phytochemical, antibacterial and sensory properties of virgin coconut oil produced

Article in Press

Coconut oil has profound health benefits but the high content of fatty acids is a concern to many consumers, several processing methods have failed to produce oil with considerable change in fatty acid content. In this study, selected lactic acid bacteria including Lactobacillus plantarum, Lactobacillus pentosus, Leuconostoc mesenteroides and Enterococcus faecium were used to ferment coconut milk for production of virgin...

Author(s):Olateru Comfort T., Popoola Bukola M., Alagbe Gbolahan O. Ajao Omobayonle

A novel method for DNA extraction from animal blood by adding bacterial lysozyme

Article in Press

The DNA quality is one of the main conditioning factors for the individual identification. Therefore, appropriate methodologies are required for their extraction. A new DNA extraction method from sheep blood was implemented based on the hexadecyl trimethyl ammonium bromide method (CTAB 2%), 1) temperature changes, cell lysis and variation of washes (CTABM), and 2) addition of an incubation process with bacterial lysozyme...

Author(s):Fidel M. Torres-Lemus, Hilda V. Silva-Rojas, Omar Hernández-Mendo, Maria M. Crosby-Galvan.

Yield and Stability Analysis of Promising Mung Bean (Vigna radiata L.) Genotypes in Ethiopia

Article in Press

Nine mung bean genotypes were evaluated for grain yield performance at four locations in 2016 main cropping season. The experiment was arranged in 3x3 lattice design with three replicates. Additive main effect and multiplicative interaction (AMMI) combined analysis of variance revealed that genotype, location and genotype x location interaction (GLI) significantly affected (p

Author(s):Hashim Samir Amsalu Berhanu Tumsa Kidane

Strain improvement of B. Parabrevis SR2729 for enhanced biodegradation of chlorpyrifos

Article in Press

Chlorpyrifos (CP) is of major concern for researchers and its biological degradation has been routinely practiced. Organophosphate hydrolase (OPH) responsible for the degradation of the agent was hyper-produced by the exposure of parental strain (PS) to EthBr for 70 minutes. Out of hundreds, the mutant strain SR2729-B (MS) showed 2.6 times greater zone of hydrolysis of CP, with OPH activity of 2.4 folds greater as compared...

Author(s):Samreen Rasool and Muhammad Anjum Zia

Tri-Phala Residue: A Low-Cost Substrate for Gallic Acid and Isoquercitrin Production by Aspergillus niger ATCC 16888 Under Solid-State Fermentation in Packed-bed Bioreactor

Article in Press

Tri-Phala residue (TR) was studied as a substrate for isoquercitrin and gallic acid productions by solid-state fermentation of A. niger in the packed bed bioreactor aerated at of 0.1, 0.3 and 0.5 vvm. The contents of biomass, isoquercitrin and gallic acid in the fermented TR along the height of the bioreactor were analyzed. The highest fungal growth rate was found on middle position in the SSF with 0.5 vvm aeration as...

Author(s):Teerin CHYSIRICHOTE and Pattarabhorn PAKAWEERACHAT

“PCR-based identification of Prevotella copri sp. Associated with New Onset Rheumatoid Arthritis (NORA)”

Article in Press

Rheumatoid arthritis (RA) is a prevalent systemic autoimmune disease, caused by a combination of genetic and environmental factors. Here we performed 16S sequencing on 30 samples from rheumatoid arthritis patients and controls. We identified the presence of Prevotella copri as strongly correlated with disease in new-onset untreated rheumatoid arthritis (NORA) patients. Increases in Prevotella abundance correlated with a...

Author(s):Veena K D , Prabhurajeshwar and Rashmi S B

Multi-Environment Yield Trials Analysis of Stability through AMMI and GGE Biplot Model of Cowpea (Vigna Unguiculata L.) Genotypes in Ethiopia

Article in Press

Developing high yielding cultivars with wider adaptability is the ultimate goal of any plant breeders. However, attaining this goal is complicated by genotype environment interaction. Sixteen cowpea lines including two standard checks were arranged in 4x4 triple lattice design and evaluated at seven locations for grain yield performance in 2016 main cropping season. Combined analysis of AMMI revealed that genotype,...

Author(s):Multi-environment trial, Cowpea, GEI, Broad adaptation

Qualitative Screening of Lipolytic Bacteria from Lipid–Rich Environmental Samples of Wolkite Town

Article in Press

Abstract The majority of lipolytic enzymes in industrial use are currently attracting an enormous attention because of their bio-technological applications. Lipase producing bacteria were isolated from five fat containing soils of oil mills, abattoir, garage, Sewage and cafeteria and were grown on nutrient agar. In order to identify the best lipid producing bacteria, the bacterial strain were screened in tributyrin...

Author(s):Mulugeta Belay, Getu Hailu , Ewenetu Azene, Eyuel Abasa, Meseret Neguse, Hayat Abdo, Getachew Tayachew

Molecular characterization of some bacteria with bioleaching potentials from Agbaja mines of Kogi State, Nigeria

Article in Press

Molecular studies were carried out on two bacterial species isolated from iron ore mines of Agbaja in Kogi State, Nigeria. This is with a view to exploring their bioleaching potentials. Preliminary morphological and biochemical tests shows that the two bacteria strains belong to Acidithiobacillus and Leptospirillum species respectively. X-ray diffraction analysis conducted on the raw iron ore gave concentrations of iron to...

Author(s): Ekpa, E,Onwurah I.N.E and Ezeanyika, L.U.S.

Disruption of C-terminal Domain Impairs in planta GCN2-dependent Phosphorylation of Arabidopsis eIF2α-subunit

Article in Press

Elucidation of mechanisms by which plants respond to stress is crucial for developing stress tolerant crops. Unlike plants, stress response mediated by eIF2α-kinases is well described in yeast and mammalian systems. We isolated a NaCl-tolerant mutant (JP2) with 35S enhancers upstream of AtGCN2. Under NaCl-stress, JP2 had 1.4 fold AtGCN2 transcripts than control. JP2 phenotype was however inconsistent, but prompted enquiry...

Author(s):Indieka S. A and Dominy P. J#

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