African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

AJMR Articles

Phytochemical screening and antibacterial properties of selected Nigerian long pepper (Capsicum frutescens) fruits

September 2015

Five varieties of Nigerian long pepper (Capsicum frutescens) fruits (var. baccatum, chacoense, fingerh, maxima and minima) were studied for their antibacterial activities.  Methods described by Association of Official Analytical Chemists (AOAC) were employed to determine the phytochemicals in the pepper fruits. The Capsicum frutescens fruits showed antibacterial activity (10.33 - 32.66 mm zones of inhibition)...

Author(s): Bello, I., Boboye, B. E. and Akinyosoye, F. A.

Expression and characterization of recombinant cellulases from an isolated Klebsiella

September 2015

Seven bacteria strains that have a comparable or higher cellulose degrading capability than Cellulomonas fimi were screened out and phylogenetically analyzed. Genes encoding 1,4-endoglucosidase (celJV) and glucoside hydrolase family 8 (celG) were cloned from Klebsiella sp. strain IV-3, and then inserted separately or simultaneously into pET-22b(+), pETDuet-1 (pDin) or pDout that combined pETDuet-1 with the pelB leader....

Author(s): Qian Li, Xiao Hong, Xiwen Chen and Defu Chen,

Investigation of specific microorganisms in the salt lakes of Southern Russia

September 2015

Specific microorganisms in extreme reservoirs with total dissolved solids of 195.7 to 585.8 g/l in Southern Russia were studied. Halophilic community of phototrophic and heterotrophic microorganisms was found. In the laboratory, an experiment was carried out to study the degradation ability of microorganisms in relation to organic content of waste water fishing enterprises - brines and bitterns (salt solutions). After...

Author(s): Bataeva, Y. V., Dzerzhinskaya, I. S., Astafyeva, O. V., Satkalieva, M. С., Yakovleva L. V., Kondratenko E. I., Magzanova D. K. and Baimuhambetova A. S.

Rhizobium tropici exopolysaccharides as carriers improve the symbiosis of cowpea-Bradyrhizobium-Paenibacillus

September 2015

Exopolysaccharides (EPS) may represent a viable inoculant carrier to replace peat and reduce production costs. In addition, the use of plant growth-promoting bacteria (PGPB), as Paenibacillus, in association with rhizobia represents a new technology that can improve the biological nitrogen fixation (BNF) and crop productivity. Thus, the present research aimed to characterize the EPS that are produced by R. tropici and...

Author(s): Artenisa Cerqueira Rodrigues, Claire Tondo Vendruscolo, Angelita da Silveira Moreira, Maria Vanilda Santos Santana, José de Paula Oliveira, Aurenivia Bonifacio and Márcia do Vale Barreto Figueiredo

Drug resistance pattern of Salmonella and Shigella species isolated from selected hospitals in Anyigba, Kogi State, Nigeria

September 2015

This work was designed to assess the status of Salmonella and Shigella infections in Anyigba, Kogi State, Nigeria with a view to making informed statements that may guide policy makers and public health experts in the containment efforts against these infections. Salmonella and Shigella species isolated from human faecal samples were examined for resistance to some antibiotic agents using disc diffusion methods. Drug...

Author(s): Helen Ojomachenwu Ocean, Emmanuel Aniebonam Eze, Chibuzor Nwadibe Eze and Matthew Chekwube Enebe

Immunity towards tuberculosis infection: A review

September 2015

Immune response represents the initial arm of host defense against Koch’s bacillus. This review describes and discusses current knowledge of the host’s immune response to Mycobacterium tuberculosis infection. To improve the diagnosis of tuberculosis, more rapid diagnostic techniques have been investigated in recent years, such as mediators, receptors and activators of immunity, gamma-interferon, tumor...

Author(s): Martínez-Romero Aurora, Ortega-Sánchez José Luis, Urtiz-Estrada Norma, Calleros-Rincón Esperanza Yasmín, Pérez-Morales Rebeca and Alba- Romero José de Jesús

Nosocomial infections in post-operative wounds due to Staphylococcus aureus and Pseudomonas aeruginosa in Benue State Nigeria

September 2015

Three hundred (300) post-operative wound swab specimens were aseptically collected from four hospitals and investigated. The four hospitals were Federal Medical Centre, Makurdi (FMCM), General Hospital, Gboko (GHG), General Hospital, Otukpo (GHO) and General Hospital North Bank, Makurdi (GHNBM). The swabs were cultured and organisms identified according to standard procedures. A prevalence rate of bacterial isolates...

Author(s): Iduh U. M., Chollom C. S., Nuhu A., Spencer T. H. I., Nura M. B., Ashcroft O. F. and Faruku N

Isolation and identification of lactic acid and non-acid lactic bacteria from “dèguè” of Western Africa traditional fermented millet-based food

September 2015

Déguè is a traditional fermented millet-based food which is consumed in Burkina Faso and other countries of West Africa. A total of 125 strains of bacteria were selected from 16 samples of déguè. Isolates were studied by determination of morphological and biochemical characteristics. Among the 125 strains of lactic acid bacteria selected, 68 strains were identified as Lactobacillus and 57...

Author(s): Cheik Amadou Tidiane OUATTARA, Marius Kounbèsiounè SOMDA, Rachel MOYEN and Alfred Sabedenedjo TRAORE

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

September 2015

Anadenanthera colubrina (Benth) Brenan, a plant known in the Northeastern Region of Brazil as angico, is widely used in traditional folk medicine to treat respiratory and inflammatory diseases. This study aimed to evaluate the antifungal activity, determine the minimum inhibitory concentration (MIC), the minimum fungicidal concentration and the fungal kinetics (death curve) in addition to the interactive effect of the...

Author(s): Luanne Eugênia Nunes, Anna Paula Porto Viana, Wilma Raianny Vieira da Rocha, Vannuty Dorneles de Sena Cunha, Raïssa Mayer Ramalho Catão and Edja Maria Melo de Brito Costa

Seroprevalence of Ehrlichia canis in dogs from Monterrey, Mexico

September 2015

Infection by Ehrlichia canis in dogs causes the worldwide tick-borne disease called canine monocytic ehrlichiosis (CME), and the presence of E. canis has been serologically demonstrated in all continents, with prevalence ranging from 0.2 to 80%. In southern Mexico, a prevalence of 44% was found, whereas in the northwest part of the country it varies from 21 to 49%. In the present study, a commercial kit for the...

Author(s): Salinas-Meléndez, J. A., Cantú-Martínez, M.A., Wong-González, A., Hernández-Escareño, J. J., Ávalos-Ramírez, R., Zárate-Ramos, J. J. and Riojas-Valdés, V. M.

Fermented milk enriched with passion fruit peel flour (passiflora edulis): Physicochemical and sensory aspects and lactic acid bacteria viability

September 2015

This study aimed to evaluate the physicochemical parameters, total and thermotolerant coliforms, lactic acid bacteria viability, instrumental color, and sensory analysis of fermented milks added to passion fruit peel flour (PFPF), throughout 29 days of storage, except composition. Four fermented milk treatments were prepared as follows: 1, fermented milk without addition of PFPF; 2, fermented milk added with 1%...

Author(s): Juliana Aparecida Célia, Marco Antônio Pereira da Silva, Kênia Borges de Oliveira, Jéssica Leal Freitas e Souza, Diene Gonçalves Souza, Ligia Campos de Moura, Richard Marins da Silva, Caroline Cagnin , Bheatriz Silva Morais de Freitas, Geovana Rocha Plácido and Márcio Caliari

Detection of Mycobacterium bovis in bovine carcasses by multiplex-PCR

September 2015

The causative agent of bovine tuberculosis (BTB) is Mycobacterium bovis, a bacterium belonging to the M. tuberculosis complex (MTC). The definitive diagnosis is achieved through isolation and identification of M. bovis from clinical samples, using a combination of traditional culture and biochemical methods, which is considered the “gold standard”. This procedure is cumbersome and time-consuming. We...

Author(s): Ricardo César Tavares Carvalho, Vinicius Silva Castro, Flávia Galindo Silvestre Silva, Carlos Adam Conte Junior, Walter Lilenbaum, Vânia Margaret Flosi Paschoalin and Eduardo Eustáquio de Souza Figueiredo

Antioxidant and in-vitro anthelminthic potentials of methanol extracts of barks and leaves of Voacanga africana and Rauwolfia vomitoria

September 2015

Voacanga africana (Stapf) and Rauwolfia vomitoria (Afzel) (Apocynaceae) are traditional plants widely used in folkloric medicine. Methanol extracts of V. africana bark (VAB) and leaves (VAL), and R. vomitoria bark (RVB) and leaves (RVL) were evaluated for antioxidant and anthelmintic potentials. The antioxidant properties of the extracts were determined by the DPPH free radical scavenging method using ascorbic acid as...

Author(s): Francis Adu, John Antwi Apenteng, William Gariba Akanwariwiak, George Henry Sam, David Ntinagyei Mintah and Edna Beyeman Bortsie

Microbiological load of yoghurt sold in Omoku schools, Rivers State, Nigeria

August 2015

Sachets of various brands of yoghurt were randomly purchased from different retail outlets within Omoku schools and its pH and microbiological quality were determined using standard method. Total bacterial count (TBC) and Coliform count were done using standard plate count method after making serial dilutions of yoghurt samples. Nutrient agar (NA) was used for enumeration of TBC. NA plates were incubated at 37°C for...

Author(s): Chimezie Gabriel Dirisu, Gloria Lily and Ebere Igwe

Effects of lithium compounds on the growth of white-rot fungi

August 2015

Identifying the most suitable lithium compounds for fungal growth is important for the lithium (Li) enrichment of mushrooms. However, enrichment thus far has been carried out using LiCl. In an effort to identify an alternative source of lithium, we investigated the effect of five lithium compounds on lag phase, growth rate and biomass of ten species of white-rot fungi. The objective of this work was to make a screening...

Author(s): Mateus D. Nunes, Willian L. Cardoso, José M. R. Luz and Maria C. M. Kasuya

Detection of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae using the ESBL NDP test and flow cytometric assay in comparison to the standard disc diffusion

August 2015

This study was undertaken to evaluate the comparison among three different assays: extended-spectrum beta-lactamases (ESBL) Nordmann/ Dortet/ Poirel (NDP) test, flow cytometric assay and disc diffusion method for the detection of ESBL production. Sixty clinical isolates of Klebsiella pneumoniae were isolated from patients’ clinical samples admitted to Suez-Canal University Hospital, Ismailia Governorate. The...

Author(s): Samaa Taha, Nahed Youssef, Amany Elkazaz and Hazem Ramadan

Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria

August 2015

The aim of this work was to determine the antibiotic susceptibility pattern of the Staphylococcus aureus isolates from orthopaedic patients and their plasmid carriage. A total number of 39 S. aureus were isolated from wound, skin and bed of orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria with the use of API STAPH identification kit. Antibiotics susceptibility test was done using disc...

Author(s): Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B.

Carbon dioxide mitigation by microalga in a vertical tubular reactor with recycling of the culture medium

August 2015

Microalgae use photosynthesis as their principal metabolic mechanism to obtain organic carbon from the inorganic carbon contained in CO2 using solar energy. This process releases oxygen into the atmosphere. The aim of this study was to determine the most appropriate biomass concentration for growing Spirulina sp. LEB 18 (Cyanobacteria) in a vertical tubular photobioreactor with biomass removal and recycling of the...

Author(s): Michele Greque de Morais, Cleber Klasener da Silva, Adriano Arruda Henrard and Jorge Alberto Vieira Costa

Congruence between the drug resistance pattern of Escherichia coli and Proteus spp. isolated from humans and those from wild animals

August 2015

As human populations grow and transform landscapes, contact with wildlife concomitantly increases. Disease emergence has been an important consequence of these contacts, with many of emerging infectious diseases in humans arising from wildlife reservoirs. Drug resistance is a very important dimension of disease emergence and tracing the source is a veritable containment strategy. Escherichia coli and Proteus spp. have...

Author(s): Eze, E. A., Mustapha, J. K., Eze, C.N. and Enebe, M. C.

Physiological and sanitary potential of peanut seed treated with cinnamon powder

August 2015

This study aimed to assess the physiological and sanitary potential of peanut seeds cv. BR1, treated with powdered cinnamon (Cinnamomum zeylanicum). The experiment was conducted in the laboratories of the Seed and Plant Pathology and Microbiology at the Federal Rural University of Pernambuco / Academic Unit of Garanhuns (UFRPE / UAG). The experimental design was completely randomized, with four replications of 50 seeds....

Author(s): Adrielle Naiana Ribeiro Soares, Edilma Pereira Gonçalves, Jeandson Silva Viana, Priscila Cordeiro Souto and Mácio Farias de Moura

Isolation and identification of Sparicotyle chrysophrii (Monogenea: Microcotylidae) from gilthead sea bream (Sparus aurata L.) in the Mediterranean Sea, Greece

August 2015

The gilthead sea bream is one of the main aquacultured fish species in the Mediterranean Sea. Sparicotyle chrysophrii is an ectoparasite specific for sea bream and able to cause mortality in the Mediterranean aquaculture system. In this study, a total of 20 gilthead sea bream were sampled for parasitological research at Heraklion Bay, Crete, Greece. Gills of the fish were dissected and examined for parasites using a...

Author(s): Jin Woo Jun

Isolation and genetic characterization of endophytic and rhizospheric microorganisms from Butia purpurascens Glassman

August 2015

Interactions between plants and microorganism are complex and can affect the establishment of plant communities and change ecosystem properties. These interactions are of interest for researchers and have many ecological and biotechnological applications. The present study is the first report on the microbial diversity associated with Butia purpurascens Glassman, and the goal was to isolate and identify the genetic...

Author(s): Cintia Faria da Silva, Jaqueline Alves Senabio, Liliane Círa Pinheiro, Marcos Antônio Soares and Edson Luiz Souchie

Response of single and co-inoculation of plant growth promoting rhizobacteria on growth, flowering and nutrient content of chrysanthemum

August 2015

A pot experiment was conducted in the screen-house of Department of Horticulture, College of Agriculture, CCS Haryana Agricultural University, Hisar during the two successive seasons of 2011-12 and 2012-13 to investigate the potential effect of different strains of Bacillus (BS1- SYB101, BS2- SB155 and BS3- SB127), Pseudomonas (PS1- WPS73, PS2- CPA152 and PS3-P20) and their combination on growth, flowering and nutrient...

Author(s): Anop Kumari, R. K. Goyal, Mahesh Choudhary and S. S. Sindhu

Isolation and characterization of Lactobacillus plantarum BLS29 as a potential probiotic starter culture for pork sausage production

August 2015

The aim of this study was to isolate and characterize lactic acid bacteria (LAB) from naturally fermented pork sausages and test them for use as a probiotic starter culture in the production of fermented sausages. LAB (n=26) were isolated from natural fermented pork sausages. Isolates were identified using 16S rDNA or Internal Transcribed Spacer (ITS) region sequencing. After that, strains were characterized based on...

Author(s): Luciana Senter,, Jamile Queiroz Pereira, Manoela Alano Vieira, Eliane Maria Zandonai Michielin and Eduardo Cesar Tondo,

Action of proteases of the nematophagous fungi Pochonia chlamydosporia on Ascaris suum eggs of collared peccary (Pecari tajacu)

August 2015

Among the parasites of domestic and wild swine, Ascaris suum stands out; a nematode that can lead to growth retardation and reduction in weight gain due to its action, especially in young animals. The objective of this study was to test the ovicidal action of proteases from Pochonia chlamydosporia (VC4) on A. suum eggs in an assay with Petri dishes. The fungus P. chlamydosporia (VC4) was grown in Erlenmeyers flasks with...

Author(s): Filippe Elias de Freitas Soares, José Humberto de Queiroz, Jackson Victor de Araújo, Maria Gorete Ramos Rodrigues, Alexandre de Oliveira Tavela, Anderson Rocha Aguiar, Tracy Lacerda,  Carolina Magri Ferraz, Maria Cristina Valdetaro Rangel,  Thiago Senna, Andreia Luíza Araújo, Tarcízio de Paula Rego, Caio Colodette Sena and Fabio Ribeiro Braga

Isolation and optimization of amylase producing bacteria and actinomycetes from soil samples of Maraki and Tewedros campus, University of Gondar, North West Ethiopia

August 2015

The objective of the present study was to isolate, identify and optimize potential amylase producing bacteria and actinomycetes from soil samples. The soil samples were collected from Maraki and Tewedros campus, University of Gondar. Isolation was done by serial dilution and spread plate method. Primary screening of amylolytic activity of the isolates was performed by starch agar plate method. The submerged state...

Author(s): Gebreselema Gebreyohannes

Lactic acid bacteria associated with the digestive tract and skin of Sea bream (Sparus aurata) cultured in Tunisia

August 2015

Thirty-seven (37) enterococcal isolates were recovered from the skin and intestines of the sea bream (Sparus aurata), the most economically important fish species of the Mediterranean sea from Tunisian fish farming sites, to investigate their antimicrobial potential. All isolates were identified to the species level using genotypic tools. An investigation employing 16S rDNA sequencing in combination with randomly...

Author(s): Ouissal Chahad Bourouni, Jorge Barros-Velàzquez, Pilar Calo-Mata and Monia El Bour

Culturable bacterial diversity and hydrolytic enzymes from drass, a cold desert in India

August 2015

Bacterial diversity of composite soil sample of drass was explored and screened for various hydrolytic enzymes. About 600 bacterial strains were isolated using six different growth media, that is, R2A, nutrient agar, King’s B media, tryptic soy agar, Luria-Bertani agar and minimal media (100 isolates picked randomly from each media). These bacterial isolates were further differentiated on the basis of...

Author(s): Puja Gupta and Jyoti Vakhlu

Occurrence of begomoviruses in cotton-vegetable agro ecosystem in India

July 2015

Begomoviruses are the important pathogens of variety of crop plants and are responsible for causing huge economic losses. It is assumed that cotton vegetable agro eco system plays a vital role in the complex etiology of the diseases caused by begomoviruses. Therefore, the present work was planned to study the relationship among begomoviruses infecting cotton, chilli, radish, tomato and papaya. For this purpose, the...

Author(s): S. I. Kaur, S. S. Kang and A. Sharma

Evaluation of hygienic-sanitary quality of honey from Apis mellifera L. obtained in semi-arid region of Piauí, Brazil

July 2015

This study aimed to evaluate the quality of honey from Apis mellifera L. obtained in Piauí, Brazil. The completely randomized design (CRD) was used in the experiments. Two treatments of honey were prepared: one from beekeepers that use Extraction Units for Bee Products (EUBP) with Best practices for beekeeping (T1), and another one from those which use EUBP without the best practices (T2). Parameters analyzed...

Author(s): Rosana Martins Carneiro Pires, Sinevaldo Gonçalves de Moura, Francisco das Chagas Cardoso Filho, Aline Marques Monte, Lúcio Fernandes Pires, Maria Cristina Affonso Lorezon, Rômulo Oliveira Barros, Maria Marlúcia Gomes Pereira and Maria Christina Sanches Muratori

Cultivation and nutritional studies of an edible mushroom from North Brazil

July 2015

The vertical mycelial growth to investigate the feasibility of Pleurotus ostreatoroseus DPUA 1720 production in lignocellulosic Amazonic residues was evaluated. Mycelial development was carried out in cupuaçu exocarp (Theobroma grandiflorum Willd Former Spreng Schum), açai seed (Euterpe oleracea) and sawdust as substrates. Each residue was supplemented with rice bran, crown and pineapple peel. The average...

Author(s): Tamiris Rio Branco da Fonseca, Taciana de Amorim Silva, Mircella Marialva Alecrim, Raimundo Felipe da Cruz Filho and Maria Francisca Simas Teixeira

Effect of processing steps and storage on microbiota and volatile amines content of salted semi preserved cuttlefish (Sepia officinalis)

July 2015

Microbial and volatile amines changes during processing steps and storage in optimal and abuse temperatures of semipreserved cuttlefish and their efficiency for quality was investigated. After osmotic treatment the initial microbial contamination and the total volatile basic nitrogen (TVBN) decreased sharply. The microbiota changed to Gram positive bacteria in which species of Bacillus and Staphylococcus were dominant....

Author(s): Ibrahima Cissé, Manuela Hernandez-Herrero, Artur X. Roig-Sagues and Buenaventura Guamis Lopes

Bacteriophage based self-assembled monolayer (SAM) on gold surface used for detection of Escherichia coli by electrochemical analysis

July 2015

Bacteriophages (or phages) are parasites that infect specific bacteria. This host-selectivity can be useful to identify bacterial contaminants in food, water, environment etc. In the present study, phage was isolated from stagnant water and cultivated by overlay method against host bacteria that is E. coli. Phage titer was calculated to be 107 pfu/ml using 10-fold dilutions. Plaque reaction activity was observed...

Author(s): Vipin Singh,  Pranay Jain and Swati Dahiya

Evaluation of antibacterial activities of Barleria Prionitis Linn

July 2015

In the present study, 1 g/ml each of Barleria prionitis leaves (BPL) and B. prionitis stem (BPS) were extracted from different solvents like petroleum ether, chloroform ethyl acetate and methanol. Ethyl acetate extract of BPL showed maximum inhibition zone on Gram positive Bacillus pumilus (9.83 mm) and methanol extract of BPS showed minimum inhibition zone on Gram negative Escherichia coli (0.16 mm). Petroleum ether...

Author(s): Patel Bharatkumar K., Chandel B. S., Chauhan H. C., Patel Kirit B., Parth Falguni M., Patel Manoj V., Patel Sanjiv I., Pandya R. P. and Shah Jignesh D.

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

July 2015

The major human fungal pathogens are species from the genus Candida, especially Candida albicans. C. albicans sample preservation from human source is essential for clinical purposes and epidemiologic studies; however, little is known about its conservation for DNA extraction and analysis. Therefore, the aim of this study was to evaluate and compare two storage conditions of C. albicans for performing DNA extraction and...

Author(s): Alessandro Menna Alves, Camila Perelló Ferrúa, Pedro Henrique de Azambuja Carvalho, Sandra Beatriz Chaves Tarquinio, Adriana Etges, Fernanda Nedel, and Rafael Guerra Lund

Diversity and distribution of endophytic bacterial community in the Noni (Morinda citrifolia L.) plant

July 2015

Noni (Morinda citrifolia L.) is a plant used by traditional cultures and also in modern health care products. Various chemical substances are derived from the plant and include, but are not limited to anthraquinone flavonol glycosides, iridoid glycosides, lipids glycosides and triterpenoids. Also commonly found on the plant are endophytic bacteria however, there are no reports on endophytic bacterial community of Noni....

Author(s): Yang Liu#, Yinhu Li#, Su Yao, Hui Wang, Yanhua Cao, Jie Li, Feirong Bai, Chuangzhao Qiu, Xin Feng, Wenkui Dai and Chi Cheng

In vitro antagonistic activity of Pseudomonas spp. against Rhizoctonia soloni

July 2015

The present research work deals with the in-vitro study of antagonistic activity of Pseudomonas spp. against plantpathogenic fungi Rhizoctonia soloni. The bacterial strains were isolated from rhizospheric soil of Korea district of Chhattisgarh. A total of 28 bacterial cultures were isolated from 25 representative soil samples collected from five blocks of Korea district of Chhattisgarh, out of which four were identified...

Author(s): Swati Rose Toppo and Preeti Tiwari

Effects of practices of maize farmers and traders in Ghana on contamination of maize by aflatoxins: Case study of Ejura-Sekyeredumase Municipality

July 2015

Contamination of maize by aflatoxins is of major concern to governments and the international community because high degrees of aflatoxin in food render the food unsafe for human consumption. The disposal of such foods also constitutes an economic loss in food production. This paper reports the findings of a study conducted during the 2013 minor maize farming season in Ejura-Sekyeredumase Municipality in the Ashanti...

Author(s): Joseph Oppong Akowuah, Lena Dzifa Mensah, Chian Chan and Anthony Roskilly

Characterization of phosphate solubilizing rhizobacteria isolated from lentil growing areas of Ethiopia

July 2015

Lentil (Lens culnaris Medik.) is an invaluable source of dietary protein for the majority of Ethiopian population. The low availability of phosphorus is one of the major soil related stresses that limit the production of this crop. This study was aimed at isolation and characterization of phosphate solubilizing bacteria from lentil growing areas of Ethiopia and evaluating their effects on growth of this crop under...

Author(s): Mulissa J. Midekssa, Carolin R. Löscher, Ruth A. Schmitz and Fassil Assefa

Phosphate solubilizing rhizospheric bacterial communities of different crops of Korea District of Chhattisgarh, India

July 2015

Occurrence of Phosphate Solubilizing Bacteria (PSB) in the rhizospheric soil of different crops of Korea District of Chhattisgarh, India was studied. Phosphate solubilizing bacteria colonize the plant roots, affect plant growth positively and add nutrients to the soil. A good number of PSB were isolated, purified and identified from twenty-five different representative soil samples collected from five blocks of Korea...

Author(s): Swati Rose Toppo and Preeti Tiwari

Antifungal and enzyme activity of endophytic fungi isolated from Ocimum sanctum and Aloe vera

July 2015

The present study was carried out to isolate endophytic fungi from Ocimum sanctum and Aloe vera and to assess their antifungal activity against Rhizoctonia solani, Fusarium oxysporum, Colletotrichum falcatum and Helminthosporium maydis. The isolated fungi were further evaluated for production of extracellular enzymes such as amylase, cellulase, chitinase, pectinase, laccase, lipase and urease. During the study 18...

Author(s): Rahul Yadav, Ajay Veer Singh, Samiksha Joshi and Manish Kumar

Occurrence of Salmonella sp. and Escherichia coli in free-living and captive wild birds from 2010-2013 in Guarapuava, Paraná, Brazil

July 2015

Birds are increasingly close to men, and many times are raised as pets. However, many times, these animals may carry and spread enterobacteria that are deleterious to human health. Salmonella sp. is considered one of the most common zoonoses in the world, causing important losses to public health. Although, Escherichia coli is an important commensal in the gastrointestinal tract of most animals, it may cause disease...

Author(s): Suelen Aparecida Suphoronski, Tânia de Freitas Raso, Nádia Cristine Weinert, Meire Christina Seki and Adriano de Oliveira Torres Carrasco

Efficacy of Aeromonas hydrophila S-layer bacterins with different protein profiles as a vaccine in Nile tilapia (Oreochromis niloticus)

July 2015

Farming of Nile tilapia is in full development throughout the world. However, an increase is still seen in the prevalence and severity of bacterial diseases. The aim of this study was to develop a vaccine against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus) based on protein patterns of S-layer proteins. The proteins were extracted using glycine and NaOH with subsequent visualization through SDS-PAGE....

Author(s): Izabela P. de Santana Lacerda, Yasmin S. Gonçalves, Samira T. Leal de Oliveira, Fábio N. Demarqui, Cristina da C. Krewer, Gisele V. Gouveia, Wagner P. Félix and Mateus M. Costa

Antifungal activity of Citharexylum quadrangulare Jacq. extracts against phytopathogenic fungi

July 2015

Antifungal activity of Citharexylum quadrangulare Jacq., an exotic tree introduced in Tunisia many years ago, was evaluated. Organic extracts using hexane, ethyl acetate and methanol solvents together with aqueous extracts tested at different concentrations were prepared from different organs (roots, stems, leaves and flowers). The fungitoxic activity for all extracts was evaluated against five phypathogenic fungi...

Author(s): Asma El Ayeb-Zakhama, Naiima Boughalleb-M’Hamdi and Fethia Harzallah-Skhiri

Assessment of fungal pathogens associated with orange spoilage

July 2015

Citrus sinensis also known as sweet orange is the most popular of the citrus fruits. It is widely cultivated in most regions of the world possessing a rich source of vitamin C, flavonoids, phenolic compounds and pectin. This research was conducted to investigate the assessments of fungal pathogens associated with orange fruit spoilage sold in five markets in Benin metropolis and the possible public health implications....

Author(s): Oviasogie, F. E., Ogofure, A. G., Beshiru, A., Ode, J. N. and Omeje F. I.

Evaluation of the antagonistic effect of six mixed cultures of lactic acid bacteria, isolated from the Ethiopian fermented milk ergo, against some foodborne pathogens inoculated into the Ethiopian cottage cheese ayib

July 2015

The antagonistic effect of mixed lactic acid bacterial cultures against foodborne pathogens (Staphylocuccus aureus ATCC 25923, Shigella boydii clinical isolate, Pseudomonas aeruginosa ATCC 25853) was evaluated in pasteurized ayib stored at room temperature. The lactic acid bacteria (LAB) were tested for acid tolerance at pH 2.0, 2.5, 3.0 and 3.5 for three and six h and for bile tolerance for 24 and 48 h at 0.3% (w/v)...

Author(s): Jermen Mamo Nigatu, Fassil Assefa Tuji and Anteneh Tesfaye Tefera

Virulence factors expressed by Mycobacterium ulcerans strains: Results of a descriptive study

July 2015

Mycobacterium ulcerans is a slow-growing mycobacterium responsible for Buruli ulcer. The pathogenic virulence of MU is being linked to the expression of toxin called Mycolactone, whose form varies according to the origin of the productive strains. Forms A and B are secreted by African and Malaysian strains, C by Australian strains and D by Asian strains. Forms E and F of mycolactone are secreted by animal mycolactone...

Author(s): AKA N’guetta, KAKOU-NGAZOA E. Solange, COULIBALY N. D.,  VAKOU Sabine, KOUADIO Kouamé, AOUSSI Serge, KADIO M. Constance, KOUAKOU Hélène, AKE Julien, N’GUESSAN Raymond and DOSSO  Mireille

Evaluation of bacteriological quality and essential elements in commercially bottled/packaged water produced and marketed in Southeastern Nigeria

July 2015

The bacteriological quality and levels of essential mineral elements and organic acids were evaluated in 33 randomly selected bottled/packaged water samples produced and marketed in Southeastern Nigeria. The microbiological assay, essential mineral elements and organic acids were determined using American Public Health Association (APHA) standard methods. The distinguishing characteristics of the microorganisms...

Author(s): Onoja Uwakwe Simon, Nweze, Innocent Emeka, Agbo Matthias Onyebuchi, Nnamani Petra Obioma, Eke Felicia Nkechi, Ivoke Njoku, Eyo Joseph Ekpenyong,  Attama Chinedu  Ifeanyi and Ejere Vincent Chikwendu

Prevalence of Brucella antibodies in marketed cow milk in Benue State, Nigeria

July 2015

Brucellosis is an endemic disease in the animal population in Nigeria and of great public health importance, particularly among livestock owners and workers who are ignorant of the risk of Brucella infection. A total of 102 milk samples comprising 62 from cattle herds and 40 from milk vendors in Benue State, Nigeria were screened for Brucella antibodies by the milk ring test (MRT). The herds for the study were randomly...

Author(s): Daniel Douglas Ior, and Cornelius Chijioke Chukwu

Optimization of bioprocess for enhanced production of alkaline protease by a Bacillus subtilis SHmIIIa through Plackett-Burman design

July 2015

Optimal conditions for the maximum production of alkaline protease by Bacillus subtilis SHmIIIa were evaluated by Plackett-Burman design. Nine process parameters namely, pH, temperature, agitation, inoculum, glucose, peptone, KH2PO4, FeSO4 and tween 20 at two levels were selected for the design. Out of nine selected parameters, six parameters (pH 9.75, agitation 225 rpm, inoculum 3.5%, glucose 5.5 g/L, peptone 3.5 g/L...

Author(s): S. Jeevan Chandra, P. Pallavi, B. S. Anuradha and S. Ram Reddy

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