African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5183

AJMR Articles

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

June 2016

The objective of this investigation was to isolate and identify Salmonella serovars present in wastewater from the University of Nigeria, Nsukka (UNN) wastewater treatment plant and to evaluate the sensitivity and precision of different microbial typing methods (conventional and molecular), in identifying and characterizing Salmonella species. A total of 100 suspected Salmonella colonies on selective media...

Author(s): Dickson Ihenrochi Dickson, Ike Anthony Chibuogwu and Ezeonu Ifeoma Maureen

Nutritional characteristics of marandu grass (Brachiaria brizantha cv. marandu) subjected to inoculation with associative diazotrophic bacteria

June 2016

Due to the search for viable and cost-effective ways to provide nitrogen for forage grasses, inoculation with diazotrophs presents high potential as an alternative to plant nutrition by reducing the use of nitrogen fertilizers. The objective of this work was to evaluate the nutrition of marandu grass (Brachiaria brizantha cv. marandu) subjected to inoculation with associative diazotrophic bacteria grown in Cerrado soil...

Author(s): Salomao Lima Guimaraes, Carolina Silva Alves dos Santos, Edna Maria Bonfim-Silva, Analy Castilho Polizel and Eder Rodrigues Batista

Screening and evaluation of herbicidal metabolites produced by Trichoderma spp.

June 2016

Laboratory bioassays were conducted to screen and evaluate the potential herbicidal metabolites produced by Trichoderma.longibrachiatum (Tr673), T. harzianum (Tr319), T. viride (Tr347) T. koningii (Tr324) and T. asperellum (Tr85) on inhibiting seed germination (SGe) and shoot/root growth (SGr/RGr) of seedlings of Porslane (Portulaca oleracea), Barnyardgrass (Echinochloa crus-galli), and Amaranthus retroflexus L. The...

Author(s): Wenfeng Kuang, Chengfan Wang and Weili Mao

Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

June 2016

Concern with traces of antibiotics found in milk and resistant bacteria has led to the search for alternatives, mainly natural products, to treat mastitis. This study aimed at evaluating the antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from mastitic milk and at analyzing their fatty acids. The following microorganisms were isolated from milk produced by...

Author(s): Cristina Hallal de Freitas, Carolina Lambrecht Goncalves, Josiara Furtado Mendes, Deborah Murowaniecki Otero, Claudio Martin Pereira de Pereira, Helenice de Lima Gonzales and Patricia da Silva Nascente

Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat

June 2016

Seroepidemiology, molecular and entomological studies of bluetongue in sheep was carried out in Gujarat, India. Out of 980 sera sample screened, 361 (36.84 %) were found positive by c-enzyme-linked immunosorbent assay (c-ELISA). Out of the 103 blood samples tested for the presence of bluetongue virus (BTV) by reverse transcription-polymerase chain reaction (RT-PCR), 3 were found positive, producing 274 bp amplicons with...

Author(s): Chauhan H. C., Bhagat A. G. , Dadawala A. I. , Patel B. K. , Chandel B. S. , Kher H. N. , Patel M. A. and Rajgor M.

Antibacterial activity of silver-nanoparticles against Staphylococcus aureus

June 2016

In this study, the antibacterial activity of silver nanoparticles (Ag-NPs) as Ag-nano disc was assessed with respect to Gram positive bacteria. Scanning electron microscope (ESM) was used for Staphylococcus aureus by measuring the diameter of inhibition zones in culture media and Ag-NPS. Eventually, gauzes containing strain were further impregnated between 2-layer nanofilms at 1, 3, 5 and 7 ppm sized 2 × 2 cm and...

Author(s): Mahsa Ydollahi, Hamed Ahari and Amir Ali Anvar

Antibiotic susceptibility patterns of Pseudomonas aeruginosa isolated from clinical and hospital environmental samples in Nasiriyah, Iraq

June 2016

A total of one hundred and forty two swab samples (92 clinical and 50 from hospital environment) were collected for the detection of Pseudomonas aeruginosa. Out of the total samples, 29 isolates of P. aeruginosa were isolated and recorded an overall prevalence rate of 20.42% (29/242) of which 18 (19.56%) were from wounds and burns swabs of patients, and 11 (22%) were from hospital environment. The highest rate of P....

Author(s): Jawad R. Al-Zaidi

Anti-HSV type-1 activity of olive leaves extract crude form acting as a microemulsion dosage form

June 2016

Olea europeae (L.) has been reported to have antibacterial, antifungal and antiviral activities. The aim of the present study is to evaluate antiviral activity of olive leaves extract (OLE) against herpes simplex viruses (HSV) type-1 virus. Screening of antiviral activity was assessed by measuring inhibition of viral-induced cytopathic effect of in vero cells of different OLE fractions that have been successively...

Author(s): Rania Abdelmonem Khattab, Alaa El-Din Shawky Hosny, Mostafa Ali Abdelkawy, Rania Hassan Fahmy and Nariman Alaa ElMenoufy,

Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections

June 2016

Many strains of Klebsiella pneumoniae can produce several virulence factors, such as siderophores, capsules, fimbrial adhesins and antibiotic resistance enzymes, which are important in the adherence, colonization, invasion and development of the infection. Therefore the aim of this study was to investigate ten virulence gens in 32 K. pneumoniae isolated from different clinical infections: urine (13), burns (12), sputum...

Author(s): Ahmed Abduljabbar Jaloob Aljanaby and Alaa Hassan Abdulhusain Alhasani

Antibacterial activity Lactobacillus plantarum isolated from fermented vegetables and investigation of the plantaricin genes

June 2016

Escherichia coli and Staphylococcus aureus are two food pathogens that cause severe food poisonings. Another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. This study aimed to evaluate the antibacterial activity of Lactobacillus plantarum against pathogenic bacteria including E. coli and S. aureus and to study if L. plantarum with antibacterial...

Author(s): Min-Hsiu Chang, Shu-Feng Hong, Jiau-Hua Chen, Mei-Fang Lin, Chin-Shuh Chen and Shu-Chen Wang

Outbreak of Burkholderia cepacia complex associated with contaminated liquid soap for hospital use: A case study

June 2016

Burkholderia cepacia complex (BCC) is gram negative bacteria which causes different types of human infections including catheter-associated infections and respiratory tract infections especially in immunocompromised  patients. This study was conducted to decide the source, to prevent the possible hospital outbreak of BCC and to determine the epidemiological source. Eleven cartridge of liquid soaps were purchased by...

Author(s): Kaya Süer, Meryem Güvenir , Barış Otlu and Emine Tunç

Microbial safety criteria and quality of traditional Egyptian Karish cheese

June 2016

Some traditional fermented dairy products like Egyptian Karish cheese and traditional fermented milk (Laban Zeer) are manufactured in farmhouses following traditional techniques using raw milk without heat treatment or addition of selected starter cultures, these products are generally designed as “artisanal”. The representative Karish samples were analyzed for their content of total microbial,...

Author(s): Sameh Awad

Special biochemical profiles of Escherichia coli strains isolated from humans and camels by the VITEK 2 automated system in Al-Ahsa, Saudi Arabia

June 2016

The VITEK 2 automated system was used for comparing biochemical characteristics of Escherichia coli isolates from human urinary tract infections (UTIs) and camel faecal samples for the first time in the study area. Identification by the system to the species level was accurate. Recovery rate of E. coli from camel specimens was 26% and for human specimens was 33%. Based on biochemical activities, human and animal strains...

Author(s): Naser A. Al Humam

Systemic elicitation of defense related enzymes suppressing Fusarium wilt in mulberry (Morus spp.)

June 2016

Antagonist and organic amendments based bioconsortia (seri bed waste+Pf1+Bs4+Th1+neem cake) was found to lead the restriction of Fusarium solani pathogen in mulberry roots mainly by inducing inherent defense enzymes. Induction of defense enzymes such as peroxidase, polyphenoloxidase, phenylalanine ammonia lyase, phenols, catalase and superoxide dismutase, was studied in mulberry plants pretreated with bioconsortia and...

Author(s): NARAYANAN Palani, PARTHASARATHY Seethapathy, RAJALAKSHMI Jeyaraman, ARUNKUMAR Kathaperumal and VANITHA Shanmugam

Diversity of MRSA SCCmec elements in Pretoria region of South Africa: The problem of variation in the assigned SCCmec types by different multiplex-polymerase chain reaction (PCR) methods and a call for an African consensus

June 2016

The SCCmec element is one of the recommended targets for MRSA characterization and several multiplex-PCR SCCmec typing methods have been developed over the past years. However, there are no data on the consistency of the SCCmec types in clinical isolates as detected by these methods. Using different previously published, commonly used M-PCR methods, this report describes the diversity of SCCmec elements in MRSA isolates...

Author(s): John F. Antiabong, Marleen M. Kock, Tsidiso G Maphanga, Adeola M. Salawu and Marthie M. Ehlers,

Biofixation of CO2 on a pilot scale: Scaling of the process for industrial application

June 2016

The use of Spirulina in CO2 biofixation, aside from its contribution to reducing the greenhouse effect, enables the use of the biomass to obtain biocompounds. In this work, Spirulina platensis was used for CO2 biofixation under different conditions of inorganic carbon. S. platensis was inoculated into 200 L bioreactors containing modified Zarrouk’s medium (concentration of the carbon was from 1.0 to 2.0 g.L-1)....

Author(s): Felipe Camerini, Michele da Rosa Andrade Zimmermann de Souza, Michele Greque de Morais, Bruna da Silva Vaz, Etiele Greque de Morais and Jorge Alberto Vieira Costa

Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

June 2016

Soybean (Glycine max L.), the most popular oil seed crop, grown in the experimental fields of Tamil Nadu Agricultural University and adjoining areas exhibited symptoms of necrosis and stunting. The symptoms were similar to bud blight of soybean caused by the Tobacco streak virus (TSV) and the disease is most prevalent throughout the country. To study the nature of infection, sap inoculation of the soybean strain induced...

Author(s): Rajamanickam, S., Ganesamurthy, K. and Karthikeyan, G.

Phylogenetic analysis of Campylobacter jejuni from human and birds sources in Iraq

June 2016

The present study was designed for a phylogenetic tree analysis of Campylobacter species as molecular method for genetic identification of Campylobacter jejuni from human and birds sources and birds and amplified by polymerase chain reaction assay using specific primers for 16S rRNA gene of Campylobacter jejuni (GenBank: EF136575.1). In this study, the multiple sequence alignment analysis and neighbor joining...

Author(s): Huda Abdal-Hadei Ali Al-Nasrawi

Hydrophobicity and specific biofilm features of Bacillus cereus spores subjected to pH stresses

June 2016

Bacillus cereus is a foodborne pathogen that often persists on food processing surfaces due the formation of spores and biofilms. Spores of 12 selected B. cereus strains from genotypes that recurred in a pasteurized milk processing line were investigated in this study, for their surface and biofilm characteristics. The main objective was to have an insight into their persistence strategies. Spore surface hydrophobicity...

Author(s): Fadila Malek

Diclofenac inhibits virulence of Proteus mirabilis isolated from diabetic foot ulcer

June 2016

Diclofenac is an analgesic and anti-inflammatory drug, used to relief the secondary complications of diabetes. Wound infections are much more serious particularly in diabetic patients. Proteus mirabilis are Gram negative rods, show a wide range of pathogenesis based on arsenal of diverse virulence factors. Recently, P. mirabilis and other Gram negative rods were isolated from diabetic foot ulcers; moreover, these...

Author(s): Wael Abdel Halim Hegazy,

Bacteriological and molecular studies on Clostridium perfringens isolated from sheep in three Egyptian provinces

May 2016

The study was intended to determine types of Clostridium perfringens and their toxins in diseased sheep with suspected enterotoxaemia, apparently healthy in contact sheep and soil in three Egyptian provinces (one year study). A total of 800 sheep were visited regularly over a period of one year to record cases of enterotoxaemia and collection of samples for bacteriological examination and toxin genotyping using...

Author(s): Elsify A., Tarabess R., Nayel M. A., Salama A., Allaam M., Abd El-Gaber M., Hassan H., Zaghawa A. and Elballal S.

Association study of single nucleotide polymorphism of human Toll like receptor 9 and susceptibility to pulmonary tuberculosis in Egyptian population

May 2016

Toll-like receptors (TLRs) are known to play important roles in human innate immune systems. Polymorphisms in and functions of TLRs have been investigated to identify associations with specific infectious diseases including tuberculosis (TB). This study was performed for 166 samples of unrelated individual's diagnosis of pulmonary tuberculosis and 98 household healthy samples. Genomic DNA was extracted from...

Author(s): Samia M. Omran, Zeinat K. Mohamed, Zainab Zakaria, Yasser M. Abd-Elmonem and Khaled E. El Gayar,

Effect of cholesterol lowering multiplex lactic acid bacteria on lipid metabolism in a hamster model

May 2016

This study aimed to investigate the effects of a probiotic complex product including Pediococcus, Lactobacillus, and Bifidobacteria on the lipid metabolism of hamsters fed a high-fat and high-cholesterol diet. Fifty male Syrian hamsters were assigned to five experimental groups: control, high-fat plus high-cholesterol diet (HFC), and HFC supplemented with low-, medium-, and high-dose of probiotic product. The hamsters...

Author(s): Cheng-Chih Tsai, Lan-Chun Chou, Shu-E Lai and Chung-Chih Huang

Effect of non-steroidal anti-inflammatory drugs and dexamethazone on the biofilm formation and expression of some adhesion-related genes of Candida albicans and Staphylococcus aureus

May 2016

Candida albicans and Staphylococcus aureus can cause many diseases which are considered in many cases life threatening infections. Their biofilm formation on the surface of medical devices is difficult to be treated. Our study evaluates the effect of some anti-inflammatory drugs on the growth, biofilm formation and the expression of some adhesion-related genes. Antimicrobial activity of the tested drugs was determined...

Author(s): Rehab Mahmoud Abd El-Baky and Sherein G. El-Gendy

Effect of tyrosol on Staphylococcus aureus antimicrobial susceptibility, biofilm formation and virulence factors

May 2016

Staphylococcus aureus is considered the most common causative agent of community and hospital-acquired infections. The isolates with increased virulence and antimicrobial resistance, especially mecithillin resistant S. aureus (MRSA), represent a challenge in hospitals and health care facilities worldwide. The effect of tyrosol, a phenolic quorum sensing compound of Candida albicans, was studied on the sensitivity of...

Author(s): Shaymaa H. Abdel-Rhman and Dina E. Rizk

Antibacterial activity of extracts of three aromatic plants from Burkina Faso against rice pathogen, Xanthomanas oryzae

May 2016

Plant extracts can have powerful antibacterial activity and may be used as biological control against important plant pathogens. This study assessed effect of extracts of Cymbopogon citratus, Eucalyptus camaldulensis and Mentha piperita against two pathovars of Xanthomonas oryzae attacking rice. Both in vitro and in vivo approaches were used for different strains of X. oryzae originating from Burkina Faso. The three...

Author(s): Wonni I., Ouedraogo S. L., Ouedraogo I. and Sanogo L.

Foliar application of Azospirillum brasilense in soybean and seed physiological quality

May 2016

Bacteria of the Azospirillum genus have considerable potential for application in agricultural systems, either in co-inoculation and foliar application to increase crop yields, due to its role in the production of phytohormones. The objective of this research was to evaluate the yield and quality of soybean seeds produced under the effect of different doses of Azospirillum brasilense bacteria applied to the leaves....

Author(s): Alan Mario Zuffo, Adriano Teodoro Bruzi, Pedro Milanez de Rezende, Maria Laene Moreira de Carvalho, Everton Vinicius Zambiazzi, Igor Oliveri Soares and Karina Barroso Silva

Effects of growth-promoting endophytic Methylobacterium on development of Citrus rootstocks

May 2016

Endophytic Methylobacterium spp. were inoculated on citrus seed and evaluated for their ability to promote growth of Citrus limonia and Citrus sunki seedlings under commercial nursery conditions. The germination rate and seedlings growth differed according to the combination between Methylobacterium species and citrus rootstock, showing that the interaction depends on their compatibility. Methylobacterium had no effect...

Author(s): Andréa Cristina Bogas, Carlos Ivan Aguilar-Vildoso, Aline Aparecida Camargo-Neves and Welington Luiz Araújo

Removal capacity of faecal pathogens from wastewater by four wetland vegetation: Typha latifolia, Cyperus papyrus, Cyperus alternifolius and Phragmites australis

May 2016

The ability of four wetland vegetation: Typha latifolia, Cyperus papyrus, Cyperus alternifolius and Phragmites mauritianus in removing pathogenic and indicator microorganisms in the wetlands were studied in bucket experiments. The findings suggested that vegetated systems can effectively reduce faecal pathogens in wastewater. Both Salmonella species and Escherichia coli removal efficiencies were above 98%. This proved...

Author(s): H. J. Kipasika, J. Buza , W. A. Smith and K. N. Njau

Penicillium citrinum VFI-51 as biocontrol agent to control charcoal rot of sorghum (Sorghum bicolor (L.) Moench)

May 2016

In our earlier investigation, a fungal isolate Penicillium citrinum VFI-51 and its secondary metabolite was reported to have antagonistic potential against Botrytis cinerea, the causative agent of Botrytis gray mold disease in chickpea. In the present investigation, P. citrinum VFI-51 was further evaluated for its antagonistic potential against Macrophomina phaseolina, the causative agent of charcoal rot in sorghum. P....

Author(s): Meesala Sreevidya, and Subramaniam Gopalakrishnan

Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae

May 2016

Entomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations:...

Author(s): Margy A. Esparza Mora, Julie G. Chacón-Orozco, Ricardo Harakava, Janaina Ribeiro Costa Rouws and Marcelo E. Fraga

Nodular bacterial endophyte diversity associated with native Acacia spp. in desert region of Algeria

May 2016

Five species of Acacia (Acacia ehrenbergiana Hayne, A. nilotica (L.) Delile, A. seyal Delile, A. tortilis (Forssk.) Hayne and A. laeta Delile) indigenous to Tamanrasset (Algeria) were investigated for their nodulation status and nodular endophytic diversity. A. ehrenbergiana showed the highest nodulation ability across the different sites in this region, indicating the widespread occurrence of compatible rhizobia in the...

Author(s): Zineb Faiza Boukhatem, Chahinez Merabet, Abdelkader Bekki, Sonia Sekkour, Odile Domergue, Robin Dupponois and Antoine Galiana

Suppression of duck hepatitis A virus Type 1 replication by lentivirus-mediated RNA-dependent RNA polymerase (RdRp) gene-specific siRNA

May 2016

To explore the application of RNAi technology for the control of duck hepatitis A virus type 1 (DHAV-1). The RNA-dependent RNA polymerase (RdRp) gene was amplified by PCR. pEGFP-RdRp was constructed for fusion expression of EGFP-RdRp protein. According to the sequence of RdRp, three gene-specific siRNAs were designed and the corresponding shRNA was inserted into pmiRZipΔ to construct pRdRp-shRNA. pRdRp-shRNA and...

Author(s): Yong-Juan Wang, Shan-Yuan Zhu, Ping-Fu Cui, An-ping Wang, Wei-Ming Hong, Hui Lu and Wei-Yong Zuo

Efficacy of plasmid curing agent on Streptomyces longsporesflavns

May 2016

Plasmid-specified traits like lactose metabolism and L-asparginase production could be eliminated from Streptomyces longsporesflavns culture during production of fermented product (Microbial succession). In this study we mainly focus on plasmid profiling and plasmid characterization from S. longsporesflavns isolated from marine soil. Then we observe the efficacy of plasmid curing agent on S. longsporesflavns....

Author(s): T. R. Sivashankari, K. Sudha, S. Barathi and V. Karthikeyan

Incidence of zoonotic Campylobacter jejuni in fast meal meat, grill chickens and symptomatic Egyptians

May 2016

Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains of C. jejuni produce a toxin (cytolethal distending toxin) that hinders the cells from dividing and produces diffuse bloody edematous exudative enteritis. The common routes of transmission are fecal-oral, person-to-person and the eating of raw or undercooked chickens or meat. This study recognizes the incidence of zoonotic...

Author(s): Hassan A. El Fadaly, Ashraf M. A. Barakat, Khaled A. Abd El-Razik, Azza S. M. Abuelnaga and Elgabry A. Elgabry

Antimicrobial activity of several Brazilian medicinal plants against phytopathogenic bacteria

May 2016

What is currently raised as a new approach in the management of plant diseases is the development and formulation of plant based biopesticides. The objective of present study is to evaluate the antibacterial activity of aqueous extracts of twelve species belonging to seven families collected from the Northeast of Brazil against four economically important phytopathogenic bacteria. Antibacterial activities of the aqueous...

Author(s): Cibele Maria Alves da Silva, Bruna Mirely da Silva Costa, Alexandre Gomes da Silva, Elineide Barbosa de Souza, Márcia Vanusa da Silva, Maria Tereza dos Santos Correia, da Silva Ana Paula Sant´Anna and Lima Vera Lúcia de Menezes

Bacteriological quality and metal levels of boreholes and hand-dug well within the Golden Star Wassa mining areas in Ghana

May 2016

This study seeks to assess the bacteriological, physicochemical and some trace metals levels in water samples from seven boreholes and a hand-dug well within communities of Golden Star Wassa Mine Limited in Ghana. Five of the sampling sites were slightly acidic and were below the lower limit of the acceptable World Health Organization (WHO) and Ghana Standard Authority (GSA) permissible guidelines for pH. However,...

Author(s): Addo M. G., Oti-Boateng W. and Obiri-Danso K.

Endophytic microorganisms from Bauhinia monandra leaves: Isolation, antimicrobial activities and interaction with galactose-specific lectin BmoLL

May 2016

Bauhinia monandra leaves are well known hypoglycemic agent in Brazilian popular medicine from which a galactose specific lectin (BmoLL) has been purified in milligram quantities. In this work, the antimicrobial activity of endophytes isolated from B. monandra leaves and the ability of BmoLL to agglutinate the microorganisms were evaluated. After disinfection, leaves of B. monandra were fragmented and distributed in...

Author(s): S. A. F. Ramos, L. C. N. Silva, M. T. S. Correia, J. M. Araújo and L. C. B. B. Coelho

Studies on leukocidins toxins and antimicrobial resistance in Staphylococcus aureus isolated from various clinical sources

May 2016

Staphylococcus aureus toxins represent a public health challenge all over the world. This study aims to analyze the prevalence of genes encoding the staphylococcal leukocidins and their correlation with antimicrobial susceptibility and the source of isolation. For this purpose, the susceptibility of 75 S. aureus isolates to 12 antimicrobial agents was investigated. The leukocidins genes (lukD, lukE, lukF and lukS) were...

Author(s): Abdel-Halem A. Abdel-hamed, Shaymaa H. Abdel-Rhman and Mohamed A. El-Sokkary

Tannase production by Aspergillus spp. UCP1284 using cashew bagasse under solid state fermentation

April 2016

Production of tannase by Aspergillus species UCP1284 was studied using solid state fermentation and cashew bagasse as substrate. Amount of 35 strains of fungi species isolated from soil of Caatinga were used for qualitative selection of strains with potential for production of tannase. Through the selected fungi, a complex study was achieved about the influence of the variables: substrate amount, initial moisture...

Author(s): Tatiana P. S. L. Liu, Tatiana S. Porto, Keila A. Moreira, Galba M. C. Takaki, Romero Brandão-Costa, Polyanna N. Herculano and Ana L. F. Porto

Evaluation of microbiological quality of raw milk, sour milk and artisanal yoghurt from Ouagadougou, Burkina Faso

April 2016

The present study was undertaken to investigate the microbiological quality of milk and artisanal dairy products sold in Ouagadougou markets. Forty-five (45) samples of milk and dairy products including raw milk, sour milk and yoghurt were collected and analyzed for aerobic mesophilic bacteria (AMB), total coliforms (TC), thermotolerant coliforms (TTC), Staphylococcus aureus (S. aureus), lactic acid bacteria (LAB) and...

Author(s): Abel Tankoano, Donatien Kabore, Aly Savadogo, Adama Soma, Nadia Fanou-Fogny, Diarra Compaore-Sereme, Joseph D. Hounhouigan and Hagrétou Sawadogo-Lingani

Antimicrobial resistance of potential pathogenic strains isolated from eggs produced by informal farms and sold in Abidjan, Ivory Coast

April 2016

The aim of this study was to investigate the microbial quality of eggs produced by informal farms and sold in most Abidjan’s markets. Thus, a preliminary investigation was conducted to evaluate the frequency of eggs consumption by the population of the study area (Abidjan). After this investigation, an analysis of the eggs microbiological quality was performed both on the shell and the eatable (egg yolk) part. The...

Author(s): Arsène Apata, Paul Attien, Gnamien S. Traore, Haziz Sina, Lamine Baba-Moussa and Rose Koffi Nevrey

Diversity of lactic acid bacteria isolated from "kpètè-kpètè" a ferment of traditional beer “tchoukoutou” produced in Benin

April 2016

The counts and identification of lactic acid bacteria isolated from the ferment “kpètè-kpètè” of the traditional beer (tchoukoutou) collected from nine large producing cities of Benin were carried out. Out of 209 strains isolated, 135 strains were purified, identified and were member of four different types based on their morphological traits, biochemical tests, API 50CH gallery...

Author(s): N’tcha Christine, Kayodé, A.P. Polycarpe, Adjanohoun Adolphe, Sina Haziz, Tanmakpi, G. Rolland, Savadogo Aly, Dicko, H. Mamoudou and Baba-Moussa Lamine

A study of high level aminoglycoside resistant enterococci

April 2016

Enterococci are a common cause of nosocomial infection and prevalence of antibiotic resistance among them is increasing. This study aimed to identify the prevalence of high level aminoglycoside resistant enterococci at Alexandria Main University Hospital. A total of 133 enterococci strains isolated from clinical specimens were all subjected to Bauer Kirby disc diffusion to detect antibiotic susceptibility pattern. High...

Author(s): Iman F. El-Ghazawy, Hadir A. S. Okasha and Sarah M. Mazloum

Plant growth promoting rhizobacteria and their potential for biocontrol of phytopathogens

April 2016

Growth promotion and disease control by rhizobacteria are complex interrelated processes that involve direct and indirect mechanisms. The mechanisms include synthesis of some metabolites (auxin, cytokinin and gibberellins), induction of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, production of siderophore, antibiotics, hydrogen cyanide hydrogen cyanide (HCN) and volatile compounds. They also include mineral...

Author(s): Elshahat M. Ramadan, Ahmed A. AbdelHafez, Enas A. Hassan and Fekria M. Saber

An overview of food safety knowledge and practices in selected schools in the city of Al Ain, United Arab Emirates

April 2016

Safe food handling in school canteens is an important practice to protect the students from food borne illnesses. The main purpose of this study was to present an overview of food safety knowledge and hygienic practices in selected schools in Al Ain city in the Emirate of Abu Dhabi, United Arab Emirates. The study consisted of three parts. The first part tackled students’ and teachers’ behavior during...

Author(s): Aisha Abushelaibi, Baboucarr Jobe, Fatima Al Dhanhani, Sharifa Al Mansoori and Feryal Al Shamsi

In vitro activities of Trichoderma species against Phytophthora parasitica and Fusarium oxysporum

April 2016

Trichoderma species usually produce chitinases and glucanases with inhibitory effects on several phytopathogens. In this work, the effects of such enzymes activities were evaluated on Phytophthora parasitica and Fusarium oxysporum. Enriched enzyme extracts were obtained from Trichoderma asperellum, Trichoderma virens, Trichoderma gamsii and Trichoderma logibrachiatum, which were grown on supplemented media with chitin...

Author(s): E. Osorio-Hernández, J. Hernández-Morales, V. Conde-Martínez, A. C. Michel-Aceves,  J. A. Lopez-Santillan and J. A. Torres-Castillo

Biocontrol of Salmonella Typhimurium growth in tomato surface by bacteriophage P22

April 2016

The aim of this research was to evaluate the effect of bacteriophage P22 on Salmonella Typhimurium in artificially inoculated tomato surface, under simulating condition of storage (10°C) and marketing (20°C) during seven days. First, we demonstrated the in vitro ability of phage P22 to reduce host population density to undetectable levels two hours after inoculation; however, S. Typhimurium was able to regrow up...

Author(s): Osvaldo López-Cuevas, Nohelia Castro-del Campo, Karina Ramirez and Cristóbal Chaidez

Asymptomatic bacteriuria: Occurrence and antibiotic susceptibility profiles among students of a tertiary institution in Ile-Ife, Nigeria

April 2016

Asymptomatic bacteriuria, a form of urinary tract infections that involves isolation of a specified quantitative count of bacteria in an appropriately collected urine specimen obtained from a person without symptoms or signs referable to urinary infection is common, but the prevalence in population varies widely with age, sex and the presence of genitourinary abnormalities. This study therefore aimed at investigating...

Author(s): Osungunna Michael Oluwole and Adeyemi Adenike Victoria

Determination of mecA expression and other resistance mechanisms in methicillin-resistant Staphylococcus aureus isolated from Oreochromis niloticus (Nile tilapia)

April 2016

Methicillin-resistant Staphylococcus aureus is a major cause of infection worldwide. Production of β-lactamases and penicillin-binding protein 2a are the two main mechanisms of resistance in S. aureus. The aim of this work was to study the mechanisms of resistance produced by the S. aureus strain isolated from Oreochromis niloticus (Nile tilapia) during an outbreak. β-Lactamases production was detected by...

Author(s): Magdy K. Soliman, Rania Abozahra, Alkhateib Y. Gaafar, Abdelgayed M. Younes and Nawal A. Affr

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