African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5183

AJMR Articles

Seroprevalence of hepatitis C virus in HIV seropositive children in Lagos, Nigeria

January 2016

Human immunodeficiency virus (HIV) may influence hepatitis C virus (HCV) disease through immunosuppression. The aim of this study was to determine the sero-prevalence of HCV in HIV sero-positive children in Lagos, Nigeria. 132 blood samples of children aged 1-15 years were collected at the HIV clinics of Lagos University Teaching Hospital (LUTH) and Lagos State University Teaching Hospital (LASUTH). Confirmatory...

Author(s): Oyefolu, Akeeb Oriowo Bola, Ashaka, Oluwaseyi Sedowhe, Anjorin, Abdulazeez Adeyemi, Salu, Olumuyiwa, Akinyemi, Kabiru Olusegun, Omilabu, Sunday and Abidoye, Gbadegesin

Polymerase chain reaction (PCR) technique compared to conventional bacteriological and serological techniques in diagnosis of human brucellosis in Egypt

January 2016

Direct diagnosis of human brucellosis is performed by cultivation with the disadvantage of being time consuming and the increase risk of laboratory acquired infection to laboratory personnel handling and performing culture procedures. Serological techniques as serum agglutination test (SAT) can also be used for diagnosis with the disadvantage of false negative and positive results that may occur. This study aims to...

Author(s): Hanaa Mohammed Refaat, Ola Ibrahim Ahmed, Safia Hamed Elabd and Mohammed Mohammed Sanad

Carbapenem resistance mechanisms among blood isolates of Klebsiella pneumoniae and Escherichia coli

January 2016

The purpose of this study was to characterize the different mechanisms of carbapenem resistance among blood isolates of Klebsiella pneumoniae and Escherichia coli. Meropenem resistant isolates were included. Antimicrobial susceptibility testing, phenotypic and genotypic detection of carbapenemase production were performed. Genetic relatedness of blaNDM-1 producers was determined by pulsed-filed gel electrophoresis...

Author(s): Mohamudha P. Rahamathulla, Belgode N. Harish, Laura Mataseje and Michael R. Mulvey

Molecular detection of resistance to rifampicin and isoniazid in tuberculosis patients in Senegal

January 2016

The aim of this study was to use molecular methods to determine the profile of resistance to rifampicin (RMP or RIF) and isoniazid (INH) in mycobacteria from tuberculosis patients in Senegal. Sputum samples (48) received by the mycobacterial laboratory of the National Antituberculosis Program (NATP) in Senegal between 2012 and 2014 were studied. Most of these samples came from patients in treatment failure or relapse...

Author(s): Dia M. L., Gueye P., Ba F., Cisse N. N., Balde O., Diouf B., Sarr M., Sow A. I. and Cisse M. F.

Phenotypic and genotypic methods for detection of metallo beta lactamases among carbapenem resistant Enterobacteriaceae clinical isolates in Alexandria Main University Hospital

January 2016

Dissemination of carbapenem resistant Enterobacteriaceae (CRE) poses a considerable threat to public health. The aim of the present work was to estimate the prevalence of metallo beta lactamases (MBL) among CRE isolated from Alexandria Main University Hospital, Egypt, to evaluate the performance of different phenotypic methods for the detection of MBL, and to investigate the local antimicrobial sensitivity profile of...

Author(s): Iman F. El-Ghazzawy, Marwa A. Meheissen and Doaa A. Younis

Isolation, characterization, and properties study of probiotic lactic acid bacteria of selected yoghurt from Bangladesh

January 2016

Probiotics, as functional food components, are recognized as safe microorganisms of viable single or mixed cultures with claimed health promoting effects on their host by improving the properties of the indigenous intestinal microflora. In the present study, a total of ten probiotic lactic acid bacteria were isolated comprising Lactobacillus acidophilus, Lactobacillus brevis, Bifidobacterium sp. and Streptococcus...

Author(s): M. Mizanur Rahman, K. Moazzem Hossain and S. M. Mahbubur Rahman

Safety characterization of Yersinia enterocolitica strains isolated from raw milk in Western Algeria

January 2016

Yersinia enterocolitica, an important food-borne enteric pathogen is associated with various clinical manifestations ranging from self-limited gastroenteritis to more invasive syndrome such as terminal ileitis and mesenteric lymphadenitis. The main aim of this study was to investigate the incidence of Y. enterocolitica in milk samples. For this purpose, one hundred (100) samples of raw cow’s milk were collected...

Author(s): Kh. Z. Hadef, Kh. Medjahdi, H. Beldjilali, I. Benamar and B. Moussa-Boudjemâa

Listeria monocytogenes in food: Control by monitoring the food processing environment

January 2016

Listeria monocytogenes is a foodborne pathogen that is widely dispersed in the environment; it is found in soil, water and plant material, and can grow at refrigeration temperature and at unfavourable conditions of pH (up to pH 4.7) and salt (up to 10%). It can persist in the harsh conditions of the food processing environment from which it can contaminate food. Listeriosis, infection with L. monocytogenes, can be mild...

Author(s): Dara Leong, Avelino Alvarez-Ordóñez, Piet Jooste and Kieran Jordan

Assessment of the antibacterial effects of Moringa peregrina extracts

December 2015

The antibacterial effect of Moringa peregrina (leaves, roots and seeds) ethanolic extracts were investigated. The effect of plant extracts were tested against three bacterial species: Escherichia coli (E. coli ATCC25922), Staphylococcus aureus (S. aureus ATCC 43300)Image and Klebsiella pneumoniae (K. pneumoniae ATCC 13883). M. peregrina ethanolic extracts showed significant antibacterial effect on the three tested...

Author(s): Ibrahim S. Majali, Sawsan A. Oran, Khaled M. A. khleifat, Haitham Qaralleh, Walid A. Rayyan and Osama Y. Althunibat

Production of aquatic feed grade algal powder from turtle breeding wastewater using a locally isolated Spirulina sp. JXSC-S1

December 2015

Spirulina sp. JXSC-S1 was isolated from a local turtle breeding wastewater storage pool in Jiaxing City, Zhejiang Province, China. Morphological studies revealed that the color, shape, spiral pitch, spiral wide and cell size of the strain fits the typical morphological characteristics of Spirulina platensis. Growth dynamics of the strain were studied in both Zarrouk medium and turtle breeding wastewater (TBW), and were...

Author(s): Jin Fei Luo and Lu Ping Jiang

Multidrug drug resistance in Staphylococcus aureus isolates from Clinical Specimens in Northern India

December 2015

Staphylococcus aureus is a well-known human pathogen that is primarily associated with nosocomial infections causing variety of diseases with increasing resistance to antibiotics. The goal of this study was to determine the prevalence and multi drug resistance patterns of both methicillin resistant S. aureus (MRSA) and methicillin susceptible S. aureus (MSSA) in the studied population. Out of 60 S. aureus isolated, 27...

Author(s): Mohammed Imran, Mohd Imran and Shaista Khan

Aflatoxin B1 in commercial granolas

December 2015

The aim of this study was to measure aflatoxin B1 in granola sold in stores in the city of Teresina, Piauí. Sixty samples of granola sold in four supermarkets were used. The mycological evaluation was carried out immediately, and then the samples were stored at -4°C, for later analysis of aflatoxin B1. Thirty-one strains of Aspergillus flavus were isolated. Two strains demonstrated capacity for...

Author(s): Marta Rejane Ribeiro dos SANTOS, Francisco das Chagas CARDOSO FILHO, Rodrigo Maciel CALVET, Rayllander Ribeiro ANDRADE, Rosana Martins Carneiro PIRES, Maria Marlúcia Gomes PEREIRA, Amilton Paulo Raposo COSTA and Maria Christina Sanches MURATORI

Antimicrobial activity of Taverniera Abyssinica A. Rich against human pathogenic bacteria and fungi

December 2015

Medicinal plants represent a rich source of antimicrobial agents. Even though hundreds of plant species have been tested for antimicrobial activities, the enormous mass of them have not been adequately evaluated. Taverniera abyssinica A. Rich is a widely used Ethiopian endemic medicinal plant commonly known under the local name of “Dingetegna”. Medicinal plant preparations are generally very popular in...

Author(s): Gemechu Ameya Buli, Abdella Gure and Engda Dessalegn

Trichoderma: A scientific approach against soil borne pathogens

December 2015

The members of the genus Trichoderma are widely used as bioagent for the control of phytopathogenic fungi in agriculture sectors. The members of this genus are reproducing asexually by the formation of cyanide and chlamydospore, in wild habitats they reproduce by formation of ascospores. Trichoderma species are well known for the production of cell wall degrading enzymes (CWDEs). These CWDEs, play a major role in...

Author(s): Mukesh Srivastava, Mohammad Shahid, Sonika Pandey,  Vipul Kumar, Anuradha Singh, Shubha Trivedi, Y. K. Srivastava and Shivram

Salmonella Typhimurium, Enteritidis, Infantis and Derby in pasty dulce de leche

December 2015

In order to evaluate the fate of Salmonella Typhimurium, Enteritidis, Infantis and Derby in pasty dulce de leche, aliquots of this sweet were experimentally contaminated with these pathogenic microorganisms at 102 bacterial cells per gram, and later analysed to evaluate microorganism count after storage for 0, 1, 2, 3, 5, 10 and 20 days. Salmonella Enteritidis was able to survive for up to 20 days in dulce de leche,...

Author(s): Débora Rodrigues Silveira, Nathalie Almeida Lopes, Helenice de Lima Gonzalez and Cláudio Dias Timm

Study of resistance mechanism in Enterobacter spp. with special reference to antimicrobials ceftazidime, moxifloxacin and nalidixic acid

December 2015

Microbial resistance to antimicrobials is spreading all over the world making it difficult to treat diseases effectively. This study aims to understand the mechanism of resistance towards the antimicrobials Ceftazidime, Moxifloxacin and Nalidixic acid, by using resistant Enterobacter spp. and sensitive Enterobacter spp. While there was complete inhibition of growth of sensitive Enterobacter spp. at 8 µg/ml...

Author(s): Nirbhavane, H. M. and Bagde, U. S.

Serogroups and antibiotics susceptibility pattern of avian pathogenic Escherichia coli strains responsible for colibacillosis in broiler breeding farms in the east of Algeria

December 2015

In East Algeria, 100 Escherichia coli strains were isolated from livers and spleens of 120 broiler carcasses that presented colibacillosis lesions at autopsy. Serogrouping of these strains demonstrated that 83% of their serogroups belong to the most pathogenic serogroups of Avian Pathogenic Escherichia coli (APEC): O1 (14%), O2 (53%) and O78 (16%). In vitro susceptibility to antimicrobials of veterinary significance was...

Author(s): Chafik Redha Messaï, Khatima Aït-Oudhia, Djamel Khelef, Taha Mossadek Hamdi, Nadia Safia Chenouf and Mohamed Ramzi Messaï

Chromogenic agar media for rapid detection of Enterobacteriaceae in food samples

December 2015

Recently, chromogenic media were used for rapid detection of pathogenic agents isolated from different materials (food, water and stool). Results of the present study indicated that specific and rapid identification of Enterobactiriaceae isolates from 46 food samples (egg, dairy products, meat, salads and cooked rice) as compared to conventional methods of biochemical test and plating selective media consume times, are...

Author(s): Manal A. H., Saad S. F., Zahraa A. J. and Saba T. H.

Symbiotic effectiveness of different indigenous Bradyrhizobium strains on selected Rj-genes harboring Myanmar soybean cultivars

December 2015

Many scientists are working to identify effective strains of rhizobia to increase nitrogen fixation and reduce nitrogen fertilizer application. Symbiotic nitrogen fixation of leguminous crops has become an alternative to nitrogenous fertilizer, due to its higher efficiency for nitrogen fixation. This experiment was conducted to select strains for improved nitrogen fixation of soybean (Glycine max L.). Bradyrhizobium...

Author(s): Aung Zaw Htwe, Takeo Yamakawa, Kyi Moe and Doan Cong Dien

Enhanced plant growth and/or nitrogen fixation by leguminous and non-leguminous crops after single or dual inoculation of Streptomyces griseoflavus P4 with Bradyhizobium strains

December 2015

Plant growth-promoting endophytic bacteria have been widely used during crop production for either single inoculation or co-inoculation with nitrogen-fixing bacteria. The effect of Streptomyces griseoflavus P4 on growth of various crops was studied and the effects of co-inoculation on plant growth, nodulation and nitrogen fixation by soybean was investigated. Pot experiments were conducted in an environmentally...

Author(s): Aung Zaw Htwe and Takeo Yamakawa

Developmental biology and infection cycle of Sclerotinia sclerotiorum causing stem rot of carnation in India

December 2015

Carnation (Dianthus caryophyllus L.) is a cut flower with greater stipulation in the world cut flower market. In India, carnations are cultivated under polyhouses in Nilgiris and Kodaikanal districts in the state of Tamil Nadu. Carnations cultivation is impeded by various diseases, among them stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary was found to be predominant in all varieties. Survey among...

Author(s): VINOD KUMAR Selvaraj, RAJESHKUMAR Ponnusamy, NAKKEERAN Sevugaperumal and ERAIVAN Arutkani Aiyanathan K.

Etiology and microbiology of periodontal diseases: A review

December 2015

Nowadays, there is a high prevalence of periodontal disease worldwide, and knowing the etiology is basic for its control. Biofilms that colonize the oral cavity are among the most complex of nature. Besides pathogenic microorganisms, genetic and environmental factors contribute to the development of this disease. Periodontal disease can be increased by several risk factors such as smoking, systemic diseases, medications...

Author(s): Vargas Segura A. I., Ilyina A., Segura Ceniceros E. P., Silva Belmares Y. and Méndez González L.

Arsenite-oxidizing bacteria isolated from arsenic contaminated surface and ground water of Uttar Pradesh, India

December 2015

Arsenic (As) contamination of surface and ground waters in Uttar Pradesh (UP) is geological in nature, even though some degree of As contamination may be due to anthropogenic activities. Little is known about the arsenite oxidizing bacteria in aquatic environments of UP. In this study we isolated the As resistant bacteria from both surface and ground waters of UP. Seven potential strains were isolated by culture method...

Author(s): Bilal Ahmad Tantry, Ibrahim Taher, Divya Shrivastava and Mudasar Nabi

Effects of plasmid acquisition by Pseudomonas aeruginosa clinical isolates on methicillin-resistant Staphylococcus aureus biofilm formation

December 2015

This work aimed to study the influence of plasmid acquisition on the mutual interaction of certain Pseudomonas aeruginosa clinical isolates and methicillin resistant Staphylococcus aureus (MRSA) clinical isolates. In this study, two mixed cultures were used: i) P. aeruginosa clinical isolate P14 which co-existed with MRSA isolate S14; and ii) P. aeruginosa clinical isolate P12 which co-existed with MRSAisolate S12. The...

Author(s): Sarra E. Saleh, Khaled M. Aboshanab, Mohammad M. Aboulwafa and Nadia A. Hassouna

Microalgae cultivation for biosurfactant production

November 2015

Microalgae can be used as both food and a source of bioactive compounds, such as oils, vitamins and biosurfactants. An important factor contributing to the production costs of bioactive compounds, such as biosurfactants, is the carbon source. One way to decrease production costs is by reducing carbon without a concomitant reduction in productivity. Biosurfactants have a wide range of industrial applications,...

Author(s): Elisângela Martha Radmann, Etiele Greque de Morais, Cibele Freitas de Oliveira, Kellen Zanfonato and Jorge Alberto Vieira Costa

Carbon and nitrogen sources differently influence Penicillium sp. HC1 conidiation in solid and liquid culture

November 2015

This work evaluates the effect of different carbon and nitrogen sources on conidiophore and conidia formation in Penicillium sp. HC1, a cellulolytic and xylanolytic fungi arousing industrial interest. A factorial design was used having two variables: A carbon source (glucose, sucrose, cassava starch, wheat bran, and rice flour) and a nitrogen source (tryptose, yeast extract, (NH4)2HPO4, and KNO3). The resulting 20...

Author(s): Ivonne Gutierréz-Rojas, Geraldine Tibasosa-Rodríguez, Nubia Moreno-Sarmiento, María Ximena Rodríguez-Bocanegra and Dolly Montoya

Antimicrobial resistance patterns of Salmonella in Ethiopia: A review

November 2015

Foods of animal origin have been consistently implicated in sporadic cases and outbreaks of salmonellosis, and are widely acknowledged to be a significant reservoir for Salmonella. The usage of growth promoting agents (GPAs) in food animal production is a major public health threat that can contribute to the emergence of antimicrobial resistance bacteria. Extensive and improper use of antimicrobial drugs in food animal...

Author(s): Jelalu Kemal, Berhanu Sibhat, Sissay Menkir, Yitagele Terefe and Yimer Muktar

Natural occurrence of Fusarium species and fumonisins in stored cassava chips

November 2015

Cassava chips are transformed products obtained following the fermentation and drying of cassava fresh roots. Once produced, chips can be stored for more than 180 days in conditions conducive for the development of toxigenic moulds. In this study, the incidence of Fusarium spp. is assessed from a set of 72 home-stored samples of cassava chips collected from farmers, during a 2-month monitoring survey period. Results...

Author(s): B. Onana, G. Essono, M. Nyegue, R. Tchikoua, Z. Ambang and M. Ayodele

Serological evidence and public health implication of hepatitis E virus infection in pigs found in Zaria, Kaduna State

November 2015

The presence of hepatitis E virus (HEV) infection has recently been demonstrated in humans, in Nigeria. Considering the zoonotic nature of the virus and its serious public health implication, it becomes imperative to determine its presence in animal reservoirs in the study area where pig’s husbandry is largely practiced. The level of awareness of the disease among pig farmers in the study area was also assessed...

Author(s): Alkali, B. R., Bello, M, B., Hussaini, S., and Onwuliri C. O.

Fungi and aflatoxins associated with wheat grains in Gaza governorates

November 2015

Cereal and its products are susceptible to mold damage during pre-and post-harvesting stages of the production. This study was carried out to determine the extent of fungal contamination and the aflatoxins AFB1, AFB2, AFG1, AFG2 levels of fifty wheat grain samples were collected randomly from different markets in the five Gaza governorates. The obtained results indicated that the most common molds isolated from...

Author(s): Ghada, S. R. Al-Sagga, Ahmed, M. Alian, Ferial, A. Ismail and Sherif, R. Mohamed

Molecular detection of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in captured Culicoides spp. in the northeastern regions of Mexico

November 2015

Midges of Culicoides spp. (Diptera: Ceratopogonidae) captured in 2013 (February to November) in four micro-regions located in the northeast of Mexico were analyzed to determine the presence of genetic sequences of the viruses of Bluetongue (BT) and Epizootic Hemorrhagic Disease (EHD) by reverse transcription–polymerase chain reaction  (RT-PCR). In all the micro-regions sampled, it was possible to detect the...

Author(s): Lozano-Rendón, J. A.,  Contreras-Balderas, A. J., Fernández-Salas, I., Zarate-Ramos, J. J. and Avalos-Ramírez, R.

Use of immunohistochemistry (IHC) in the detection of Newcastle disease virus (NDV) in experimentally and naturally infected birds

November 2015

The objective of the present study was to standardize an immunohistochemical reaction (IHC) for the diagnosis of Newcastle disease virus (NDV) in tissue samples. In order to achieve this aim, pigeons (Columba livia) and chicken (Gallus gallus) were experimentally infected with a pathogenic sample of NDV (São Joao do Meriti strain). The IHC reaction was standardized and tested in samples of lung, liver, spleen and...

Author(s): Adriano de Oliveira Torres Carrasco, Meire Christina Seki, Tiago Wilson Patriarca Mineo, Jayme Augusto Peres, Laura Marques Knychala, Mario Henrique Alves, Jyan Lucas Benevenute, Priscila Ikeda and Aramis Augusto Pinto

Is the recently described Macrophomina pseudophaseolina pathogenically different from Macrophomina phaseolina?

November 2015

Charcoal rot disease causes heavy yield losses to many hosts including cowpea (Vigna unguiculata) and groundnut (Arachis hypogea) in Senegal. The causal agent of the disease was a longtime considered to be Macrophomina phaseolina. However, a new Macrophomina species, Macrophomina pseudophaseolina was reported recently to also cause charcoal rot disease alone or in association with M. phaseolina on several hosts in...

Author(s): Mbaye Ndiaye, Mame P. Sarr, Ndiaga Cisse and Ibrahima Ndoye

Diversity and antibiotic susceptibilities of bacterial species from surfaces of publicly used equipment in a medical education setting

November 2015

Medical and health science colleges have firm links to hospitals and health services centres through clinical training activities. Such colleges are expected to house microorganisms of nosocomial-infection significance that can be transmitted to and from students’ hands and bodies through publicly used equipment, including food and drink vending machines, elevators and computer keyboards. This study aimed to...

Author(s): Nazik Mustafa, Badriah Ibrahim, Mariam Jasim, Mira Salim, Qumasha Abdalla and Shaima Saleh

Biofixation of carbon dioxide from coal station flue gas using Spirulina sp. LEB 18 and Scenedesmus obliquus LEB 22

November 2015

The atmospheric concentration of CO2, the main greenhouse gas (GHG), has increased rapidly since the beginning of the industrial age. Anthropogenic emissions from coal-fired power plants are responsible for approximately 7% (w/w) of global CO2 emissions. Microalgae CO2biofixation is a technology that reduces GHGs based on the use of solar energy through photosynthesis to capture and use the CO2 that is produced by...

Author(s): Jorge Alberto Vieira Costa, Michele Greque de Morais, Elisângela Martha Radmann, FabrícioButierres Santana, Felipe Camerini, Michele da Rosa Andrade Zimmerman de Souza⊃, Adriano Arruda Henrard⊃, Ana Priscila Centeno da Rosa⊃ and Lúcio Brusch

Phenotypic and genotypic characterization of Staphylococcus aureus isolates recovered from bovine milk in central highlands of Ethiopia

November 2015

Antimicrobial resistance is becoming an extremely serious global problem. The goal of this study was to determine the prevalence and phenotypic and genotypic characteristics of Staphylococcus aureus isolated from milk and milk product samples in Ethiopia and also to determine the presence of methicillin resistant Staphylococcus aureus (MRSA). A total of 577 milk and milk product samples were collected from central...

Author(s): Eyasu Tigabu, Tesfu Kassa, Daniel Asrat, Haile Alemayehu, Thomas Sinmegn, Pamela R.F. Adkins and Wondwossen Gebreyes

Diversity and distribution of soybean-nodulating bradyrhizobia isolated from major soybean-growing regions in Myanmar

October 2015

Rhizobia play an important role in plant nutrition by fixing nitrogen that is subsequently available for uptake by plants. In Myanmar, it has been difficult to characterize the nitrogen-fixing indigenous bradyrhizobia strains. In this study, 120 strains were isolated from five major soybean-growing regions of Myanmar. The strains were characterized based on sequence analysis of the 16S-23S rRNA internal transcribed...

Author(s): Aung Zaw Htwe, Takeo Yamakawa, Papa Saliou Sarr and Tomoyuki Sakata

Antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from chickens in a diagnostic laboratory

October 2015

The aim of this study was to determine the resistance profile of 24 Campylobacter jejuni and 16 Campylobacter coli isolates from chickens in a diagnostic laboratory in Nigeria. Susceptibility testing was done by a broth microdilution MIC method with MICRONAUT – S anaerob test plates (Merlin Diagnostika, GmbH, Germany). MIC assay was performed according to CLSI (formally NCCLS) methods. Resistance to ciprofloxacin...

Author(s): Sati Samuel Ngulukun, Stephen Ike Oboegbulem, Idowu Fagbamila, Israel Barde

Efficacy of oregano oil as a biocide agent against pathogens in vitro, using lux reporter gene technology

October 2015

This study aimed to determine the antimicrobial activity and strength of kill of Himalayan oregano oil (HOO) across a range of dilutions typically formulated for potential topical use in healthcare settings, using bioluminescent constructs of bacterial pathogens as real time biosensors for rapid bactericidal monitoring. Escherichia coli, Pseudomonas aeruginosa and Methicillin sensitive Staphylococcus aureus, previously...

Author(s): Nahla Omer ELtai, Vyvyan Salisbury and John Greenman

Antibacterial and hemolytic activities of Mimosa tenuiflora (Willd) Poir. (Mimosoidea)

October 2015

Mimosa tenuiflora is a shrub-sized plant native of the Northeast region of Brazil where it is popularly known as “jurema preta” and is widely used in folk medicine, especially the stem bark extract mixtures. Due to its high content of tannins and flavonoids, it is considered to have anti-inflammatory and antimicrobial activity. The antimicrobial activity of the ethanolic extracts of M. tenuiflora (EEMt) was...

Author(s): Sônia Carmem de Morais Leite, Cássio Ilan Soares Medeiros, Paula Christianne Gomes Gouveia Souto Maia, Maria Iranilda Silva Magalhães, Francisco Orlando Rafael Freitas, Hilzeth de Luna Freire Pessôa, Tiago Bezerra de Sá Sousa Nogueira, Alanna Michely Batista de Morais, Vandezita Dantas de Medeiros Mazzaro, Vanessa Passos Brustein and Geraldo Gonçalves de Almeida Filho

Efficacy of Acinetobacter baumannii bacteriophage cocktail on Acinetobacter baumannii growth

October 2015

Acinetobacter baumannii is an opportunistic pathogen which is a major cause of nosocomial infection. Bacteriophages are bacterial viruses that are used as alternative agents in the treatment of multidrug-resistant bacterial infections. In this research, our purpose was to investigate the efficacy of a cocktail of five bacteriophages. Ten (10) bacteriophage isolates were determined for the host range analysis and five...

Author(s): Thawatchai Kitti, Rapee Thummeepak, Udomluk Leungtongkam, Duangkamol Kunthalert, and Sutthirat Sitthisak

Shigellosis associated with appendicitis and peritonitis

October 2015

Appendix perforation is one of the many causes of secondary peritonitis. In this report, a case of peritonitis caused by Shigella sonnei in a 7-year-old child was presented. A 7-year-old male patient was presented with tenderness in the right lower quadrant and positive rebound tenderness test on physical examination. Abdominal ultrasonography and computed tomography revealed appendicular colitis and intraabdominal free...

Author(s): Hakan Sezgin Sayiner, Musa Abes, Burak Ekrem Citil, Hasan Ogunc Apaydin, Sadik Akgun, Ali Haydar Baykan, Halil Ibrahim Erdogdu, Haci Mehmet Inan and Selcuk Aksoz

Potential pathogenic bacterial contaminants of shared utility devices in a university setting at Al-Hofuf, Saudi Arabia

October 2015

The microbial contamination of shared devices in work places could serve as potential sources for community acquired infections. This study investigated the potential bacterial pathogens in a university work place. Swab samples collected from office and toilet doors handles/knobs, washroom tap heads, elevator buttons and computer keyboards were plated out and isolates were identified using the Vitek 2 compact automated...

Author(s): Lorina Ineta Badger-Emeka, Abdulrahman Abdulhadi Al-Sultan,  Hajer Salman Al-Dehailan,  Nouf Khaled Al-Humini, Fatimah Ahamd Al- Najja and Hani Mohammed Al-Farhan

Antimicrobial susceptibility pattern of Gram-negative bacilli isolated from a Teaching Hospital in Jeddah, Saudi Arabia

October 2015

Gram-negative bacilli (GNB) are commonly implicated in clinical diseases. However, with their increasing resistance to antimicrobial agents, treatment becomes a challenge. This study has been conducted at King Abdulaziz University Hospital in Jeddah, Saudi Arabia over a period of one month from July to August 2011. Identification and antibiotic sensitivity tests of GNB were performed using standard microbiological...

Author(s): Razina M. Zaman, Magda M. Aly and Noof R. Helmi

Prevalence of Mycobacterium leprae in the environment: A review

October 2015

The purpose of this review was to study the presence of Mycobacterium leprae in the environment and its relation with meteorological variables such as temperature and humidity. There are reports, which provide evidence that meteorological factors such as temperature and soil humidity can influence the dynamics of M. leprae. However, leprosy cases are registered both in the rainy and dry seasons, indicating that M....

Author(s): Elderson Mariano de Souza VALOIS, Franciely Maria Carrijo CAMPOS and Eliane IGNOTTI

Antibacterial in vitro assays of new -aminoethers and derivatives against Gram-negative and Gram-positive pathogenic bacteria

October 2015

A growth inhibition effect against four Gram-negative (Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella pneumoniae and Escherichia coli) and three Gram-positive (Staphylococcus aureus, Bacillus cereus and Enterococcus faecalis) pathogenic bacteria was observed for 19 of 20 tested synthetic compounds (that is seven -aminoethers, nine -aminoalcohols and four allylamines). According to the results, the...

Author(s): Natalia Duque, Mary Carabali, Neyla Benítez, Juan Castillo and Rodrigo Abonia

Effects of vegetation and seasonality on bacterial communities in Amazonian dark earth and adjacent soils

October 2015

Amazonian Dark Earth (ADE) in the Brazilian Amazon is the main evidence left by pre-Columbian indigenous populations indicating that infertile soils can be transformed into highly fertile ground. Changes in vegetation cover and seasonality are likely to influence microbial communities; however, little is known about these effects on ADE. Therefore, this study compared the effects of two land use systems in ADE and...

Author(s): Amanda Barbosa Lima, Fabiana de Souza Cannavan, Mariana Gomes Germano, Francisco Dini-Andreote, Alessandra Monteiro de Paula, Julio Cezar Franchini, Wenceslau Geraldes Teixeira and Siu Mui Tsai

Growth and anti-listerial activity of a nisin Z producer in a pork lean meat broth fermentation system

September 2015

Listeria monocytogenes is widely distributed in nature and has been isolated from numerous sources such as meat and fermented meat products. This pathogenic microorganism can resist conditions of low pH, low water activity (Aw), high salt (NaCl) concentrations and the presence of sodium nitrite, being able to survive the commercial sausage manufacturing process. The aim of this work was to evaluate the antilisterial...

Author(s): Rosinéa Aparecida de Paula, Ana Andréa Teixeira Barbosa, Sílvia Regina Sartori Machado, Margarete Alice Fontes Saraiva, Célia Alencar de Moraes and Hilário C. Mantovani

In vitro antibacterial activity of Baillonella toxisperma (Pierre) extracts against Staphylococcus aureus, Salmonella typhi, Proteus mirabilis and Bacillus cereus F3748

September 2015

This study evaluated the in vitro antibacterial activity of the ethyl acetate, acetone, methanol and hydro-ethanol mixture (2: 8) extracts of the leaves and stem-barks of Baillonella toxisperma (Pierre), harvested in the East and center regions of Cameroon, on Staphylococcus aureus, Salmonella typhi, Proteus  mirabilis and  Bacillus  cereus  F3748. These bacteria are usually responsible for diarrheal...

Author(s): S. H. Riwom Essama, J. Ngo Mbing, M. A. Nyegue, C. Ndoye Foe, M. Fodouop, S. P. Bouopda Tamo, D. E Pegnyemb and F. X. Etoa

Expression and characterization of recombinant cellulases from an isolated Klebsiella

September 2015

Seven bacteria strains that have a comparable or higher cellulose degrading capability than Cellulomonas fimi were screened out and phylogenetically analyzed. Genes encoding 1,4-endoglucosidase (celJV) and glucoside hydrolase family 8 (celG) were cloned from Klebsiella sp. strain IV-3, and then inserted separately or simultaneously into pET-22b(+), pETDuet-1 (pDin) or pDout that combined pETDuet-1 with the pelB leader....

Author(s): Qian Li, Xiao Hong, Xiwen Chen and Defu Chen,

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