African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5145

AJMR Articles

Efficacy of oregano oil as a biocide agent against pathogens in vitro, using lux reporter gene technology

October 2015

This study aimed to determine the antimicrobial activity and strength of kill of Himalayan oregano oil (HOO) across a range of dilutions typically formulated for potential topical use in healthcare settings, using bioluminescent constructs of bacterial pathogens as real time biosensors for rapid bactericidal monitoring. Escherichia coli, Pseudomonas aeruginosa and Methicillin sensitive Staphylococcus aureus, previously...

Author(s): Nahla Omer ELtai, Vyvyan Salisbury and John Greenman

Antibacterial and hemolytic activities of Mimosa tenuiflora (Willd) Poir. (Mimosoidea)

October 2015

Mimosa tenuiflora is a shrub-sized plant native of the Northeast region of Brazil where it is popularly known as “jurema preta” and is widely used in folk medicine, especially the stem bark extract mixtures. Due to its high content of tannins and flavonoids, it is considered to have anti-inflammatory and antimicrobial activity. The antimicrobial activity of the ethanolic extracts of M. tenuiflora (EEMt) was...

Author(s): Sônia Carmem de Morais Leite, Cássio Ilan Soares Medeiros, Paula Christianne Gomes Gouveia Souto Maia, Maria Iranilda Silva Magalhães, Francisco Orlando Rafael Freitas, Hilzeth de Luna Freire Pessôa, Tiago Bezerra de Sá Sousa Nogueira, Alanna Michely Batista de Morais, Vandezita Dantas de Medeiros Mazzaro, Vanessa Passos Brustein and Geraldo Gonçalves de Almeida Filho

Efficacy of Acinetobacter baumannii bacteriophage cocktail on Acinetobacter baumannii growth

October 2015

Acinetobacter baumannii is an opportunistic pathogen which is a major cause of nosocomial infection. Bacteriophages are bacterial viruses that are used as alternative agents in the treatment of multidrug-resistant bacterial infections. In this research, our purpose was to investigate the efficacy of a cocktail of five bacteriophages. Ten (10) bacteriophage isolates were determined for the host range analysis and five...

Author(s): Thawatchai Kitti, Rapee Thummeepak, Udomluk Leungtongkam, Duangkamol Kunthalert, and Sutthirat Sitthisak

Antimicrobial susceptibility pattern of Gram-negative bacilli isolated from a Teaching Hospital in Jeddah, Saudi Arabia

October 2015

Gram-negative bacilli (GNB) are commonly implicated in clinical diseases. However, with their increasing resistance to antimicrobial agents, treatment becomes a challenge. This study has been conducted at King Abdulaziz University Hospital in Jeddah, Saudi Arabia over a period of one month from July to August 2011. Identification and antibiotic sensitivity tests of GNB were performed using standard microbiological...

Author(s): Razina M. Zaman, Magda M. Aly and Noof R. Helmi

Potential pathogenic bacterial contaminants of shared utility devices in a university setting at Al-Hofuf, Saudi Arabia

October 2015

The microbial contamination of shared devices in work places could serve as potential sources for community acquired infections. This study investigated the potential bacterial pathogens in a university work place. Swab samples collected from office and toilet doors handles/knobs, washroom tap heads, elevator buttons and computer keyboards were plated out and isolates were identified using the Vitek 2 compact automated...

Author(s): Lorina Ineta Badger-Emeka, Abdulrahman Abdulhadi Al-Sultan,  Hajer Salman Al-Dehailan,  Nouf Khaled Al-Humini, Fatimah Ahamd Al- Najja and Hani Mohammed Al-Farhan

Shigellosis associated with appendicitis and peritonitis

October 2015

Appendix perforation is one of the many causes of secondary peritonitis. In this report, a case of peritonitis caused by Shigella sonnei in a 7-year-old child was presented. A 7-year-old male patient was presented with tenderness in the right lower quadrant and positive rebound tenderness test on physical examination. Abdominal ultrasonography and computed tomography revealed appendicular colitis and intraabdominal free...

Author(s): Hakan Sezgin Sayiner, Musa Abes, Burak Ekrem Citil, Hasan Ogunc Apaydin, Sadik Akgun, Ali Haydar Baykan, Halil Ibrahim Erdogdu, Haci Mehmet Inan and Selcuk Aksoz

Effects of vegetation and seasonality on bacterial communities in Amazonian dark earth and adjacent soils

October 2015

Amazonian Dark Earth (ADE) in the Brazilian Amazon is the main evidence left by pre-Columbian indigenous populations indicating that infertile soils can be transformed into highly fertile ground. Changes in vegetation cover and seasonality are likely to influence microbial communities; however, little is known about these effects on ADE. Therefore, this study compared the effects of two land use systems in ADE and...

Author(s): Amanda Barbosa Lima, Fabiana de Souza Cannavan, Mariana Gomes Germano, Francisco Dini-Andreote, Alessandra Monteiro de Paula, Julio Cezar Franchini, Wenceslau Geraldes Teixeira and Siu Mui Tsai

Antibacterial in vitro assays of new -aminoethers and derivatives against Gram-negative and Gram-positive pathogenic bacteria

October 2015

A growth inhibition effect against four Gram-negative (Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella pneumoniae and Escherichia coli) and three Gram-positive (Staphylococcus aureus, Bacillus cereus and Enterococcus faecalis) pathogenic bacteria was observed for 19 of 20 tested synthetic compounds (that is seven -aminoethers, nine -aminoalcohols and four allylamines). According to the results, the...

Author(s): Natalia Duque, Mary Carabali, Neyla Benítez, Juan Castillo and Rodrigo Abonia

Prevalence of Mycobacterium leprae in the environment: A review

October 2015

The purpose of this review was to study the presence of Mycobacterium leprae in the environment and its relation with meteorological variables such as temperature and humidity. There are reports, which provide evidence that meteorological factors such as temperature and soil humidity can influence the dynamics of M. leprae. However, leprosy cases are registered both in the rainy and dry seasons, indicating that M....

Author(s): Elderson Mariano de Souza VALOIS, Franciely Maria Carrijo CAMPOS and Eliane IGNOTTI

Growth and anti-listerial activity of a nisin Z producer in a pork lean meat broth fermentation system

September 2015

Listeria monocytogenes is widely distributed in nature and has been isolated from numerous sources such as meat and fermented meat products. This pathogenic microorganism can resist conditions of low pH, low water activity (Aw), high salt (NaCl) concentrations and the presence of sodium nitrite, being able to survive the commercial sausage manufacturing process. The aim of this work was to evaluate the antilisterial...

Author(s): Rosinéa Aparecida de Paula, Ana Andréa Teixeira Barbosa, Sílvia Regina Sartori Machado, Margarete Alice Fontes Saraiva, Célia Alencar de Moraes and Hilário C. Mantovani

In vitro antibacterial activity of Baillonella toxisperma (Pierre) extracts against Staphylococcus aureus, Salmonella typhi, Proteus mirabilis and Bacillus cereus F3748

September 2015

This study evaluated the in vitro antibacterial activity of the ethyl acetate, acetone, methanol and hydro-ethanol mixture (2: 8) extracts of the leaves and stem-barks of Baillonella toxisperma (Pierre), harvested in the East and center regions of Cameroon, on Staphylococcus aureus, Salmonella typhi, Proteus  mirabilis and  Bacillus  cereus  F3748. These bacteria are usually responsible for diarrheal...

Author(s): S. H. Riwom Essama, J. Ngo Mbing, M. A. Nyegue, C. Ndoye Foe, M. Fodouop, S. P. Bouopda Tamo, D. E Pegnyemb and F. X. Etoa

A novel method to optimize culture conditions for biomass and sporulation of the nematophagous fungus Metarhizium anisopliae SQZ-1-21 and the entomopathogenic fungus Metarhizium anisopliae RS-4-1

September 2015

Metarhizium anisopliae is a fungus that offers great potential for the biocontrol of a wide range of host species. In this study, we developed a novel two-stage cultivation method to optimize nutrition and environmental conditions for M. anisopliae SQZ-1-21 and M. anisopliae RS-4-1. Both species were cultured on basal medium (19.00 g sucrose, 4.06 g soy peptone, 1.00 g K2HPO4, 0.50 g KCl, 0.50 g MgSO4, 0.01 g FeSO4 and...

Author(s): Li Gao

Phytochemical screening and antibacterial properties of selected Nigerian long pepper (Capsicum frutescens) fruits

September 2015

Five varieties of Nigerian long pepper (Capsicum frutescens) fruits (var. baccatum, chacoense, fingerh, maxima and minima) were studied for their antibacterial activities.  Methods described by Association of Official Analytical Chemists (AOAC) were employed to determine the phytochemicals in the pepper fruits. The Capsicum frutescens fruits showed antibacterial activity (10.33 - 32.66 mm zones of inhibition)...

Author(s): Bello, I., Boboye, B. E. and Akinyosoye, F. A.

Expression and characterization of recombinant cellulases from an isolated Klebsiella

September 2015

Seven bacteria strains that have a comparable or higher cellulose degrading capability than Cellulomonas fimi were screened out and phylogenetically analyzed. Genes encoding 1,4-endoglucosidase (celJV) and glucoside hydrolase family 8 (celG) were cloned from Klebsiella sp. strain IV-3, and then inserted separately or simultaneously into pET-22b(+), pETDuet-1 (pDin) or pDout that combined pETDuet-1 with the pelB leader....

Author(s): Qian Li, Xiao Hong, Xiwen Chen and Defu Chen,

Investigation of specific microorganisms in the salt lakes of Southern Russia

September 2015

Specific microorganisms in extreme reservoirs with total dissolved solids of 195.7 to 585.8 g/l in Southern Russia were studied. Halophilic community of phototrophic and heterotrophic microorganisms was found. In the laboratory, an experiment was carried out to study the degradation ability of microorganisms in relation to organic content of waste water fishing enterprises - brines and bitterns (salt solutions). After...

Author(s): Bataeva, Y. V., Dzerzhinskaya, I. S., Astafyeva, O. V., Satkalieva, M. С., Yakovleva L. V., Kondratenko E. I., Magzanova D. K. and Baimuhambetova A. S.

Rhizobium tropici exopolysaccharides as carriers improve the symbiosis of cowpea-Bradyrhizobium-Paenibacillus

September 2015

Exopolysaccharides (EPS) may represent a viable inoculant carrier to replace peat and reduce production costs. In addition, the use of plant growth-promoting bacteria (PGPB), as Paenibacillus, in association with rhizobia represents a new technology that can improve the biological nitrogen fixation (BNF) and crop productivity. Thus, the present research aimed to characterize the EPS that are produced by R. tropici and...

Author(s): Artenisa Cerqueira Rodrigues, Claire Tondo Vendruscolo, Angelita da Silveira Moreira, Maria Vanilda Santos Santana, José de Paula Oliveira, Aurenivia Bonifacio and Márcia do Vale Barreto Figueiredo

Drug resistance pattern of Salmonella and Shigella species isolated from selected hospitals in Anyigba, Kogi State, Nigeria

September 2015

This work was designed to assess the status of Salmonella and Shigella infections in Anyigba, Kogi State, Nigeria with a view to making informed statements that may guide policy makers and public health experts in the containment efforts against these infections. Salmonella and Shigella species isolated from human faecal samples were examined for resistance to some antibiotic agents using disc diffusion methods. Drug...

Author(s): Helen Ojomachenwu Ocean, Emmanuel Aniebonam Eze, Chibuzor Nwadibe Eze and Matthew Chekwube Enebe

Immunity towards tuberculosis infection: A review

September 2015

Immune response represents the initial arm of host defense against Koch’s bacillus. This review describes and discusses current knowledge of the host’s immune response to Mycobacterium tuberculosis infection. To improve the diagnosis of tuberculosis, more rapid diagnostic techniques have been investigated in recent years, such as mediators, receptors and activators of immunity, gamma-interferon, tumor...

Author(s): Martínez-Romero Aurora, Ortega-Sánchez José Luis, Urtiz-Estrada Norma, Calleros-Rincón Esperanza Yasmín, Pérez-Morales Rebeca and Alba- Romero José de Jesús

In vitro evaluation of antifungal activity and interactive effect of Anadenanthera colubrina (Benth)

September 2015

Anadenanthera colubrina (Benth) Brenan, a plant known in the Northeastern Region of Brazil as angico, is widely used in traditional folk medicine to treat respiratory and inflammatory diseases. This study aimed to evaluate the antifungal activity, determine the minimum inhibitory concentration (MIC), the minimum fungicidal concentration and the fungal kinetics (death curve) in addition to the interactive effect of the...

Author(s): Luanne Eugênia Nunes, Anna Paula Porto Viana, Wilma Raianny Vieira da Rocha, Vannuty Dorneles de Sena Cunha, Raïssa Mayer Ramalho Catão and Edja Maria Melo de Brito Costa

Isolation and identification of lactic acid and non-acid lactic bacteria from “dèguè” of Western Africa traditional fermented millet-based food

September 2015

Déguè is a traditional fermented millet-based food which is consumed in Burkina Faso and other countries of West Africa. A total of 125 strains of bacteria were selected from 16 samples of déguè. Isolates were studied by determination of morphological and biochemical characteristics. Among the 125 strains of lactic acid bacteria selected, 68 strains were identified as Lactobacillus and 57...

Author(s): Cheik Amadou Tidiane OUATTARA, Marius Kounbèsiounè SOMDA, Rachel MOYEN and Alfred Sabedenedjo TRAORE

Nosocomial infections in post-operative wounds due to Staphylococcus aureus and Pseudomonas aeruginosa in Benue State Nigeria

September 2015

Three hundred (300) post-operative wound swab specimens were aseptically collected from four hospitals and investigated. The four hospitals were Federal Medical Centre, Makurdi (FMCM), General Hospital, Gboko (GHG), General Hospital, Otukpo (GHO) and General Hospital North Bank, Makurdi (GHNBM). The swabs were cultured and organisms identified according to standard procedures. A prevalence rate of bacterial isolates...

Author(s): Iduh U. M., Chollom C. S., Nuhu A., Spencer T. H. I., Nura M. B., Ashcroft O. F. and Faruku N

Antioxidant and in-vitro anthelminthic potentials of methanol extracts of barks and leaves of Voacanga africana and Rauwolfia vomitoria

September 2015

Voacanga africana (Stapf) and Rauwolfia vomitoria (Afzel) (Apocynaceae) are traditional plants widely used in folkloric medicine. Methanol extracts of V. africana bark (VAB) and leaves (VAL), and R. vomitoria bark (RVB) and leaves (RVL) were evaluated for antioxidant and anthelmintic potentials. The antioxidant properties of the extracts were determined by the DPPH free radical scavenging method using ascorbic acid as...

Author(s): Francis Adu, John Antwi Apenteng, William Gariba Akanwariwiak, George Henry Sam, David Ntinagyei Mintah and Edna Beyeman Bortsie

Detection of Mycobacterium bovis in bovine carcasses by multiplex-PCR

September 2015

The causative agent of bovine tuberculosis (BTB) is Mycobacterium bovis, a bacterium belonging to the M. tuberculosis complex (MTC). The definitive diagnosis is achieved through isolation and identification of M. bovis from clinical samples, using a combination of traditional culture and biochemical methods, which is considered the “gold standard”. This procedure is cumbersome and time-consuming. We...

Author(s): Ricardo César Tavares Carvalho, Vinicius Silva Castro, Flávia Galindo Silvestre Silva, Carlos Adam Conte Junior, Walter Lilenbaum, Vânia Margaret Flosi Paschoalin and Eduardo Eustáquio de Souza Figueiredo

Seroprevalence of Ehrlichia canis in dogs from Monterrey, Mexico

September 2015

Infection by Ehrlichia canis in dogs causes the worldwide tick-borne disease called canine monocytic ehrlichiosis (CME), and the presence of E. canis has been serologically demonstrated in all continents, with prevalence ranging from 0.2 to 80%. In southern Mexico, a prevalence of 44% was found, whereas in the northwest part of the country it varies from 21 to 49%. In the present study, a commercial kit for the...

Author(s): Salinas-Meléndez, J. A., Cantú-Martínez, M.A., Wong-González, A., Hernández-Escareño, J. J., Ávalos-Ramírez, R., Zárate-Ramos, J. J. and Riojas-Valdés, V. M.

Fermented milk enriched with passion fruit peel flour (passiflora edulis): Physicochemical and sensory aspects and lactic acid bacteria viability

September 2015

This study aimed to evaluate the physicochemical parameters, total and thermotolerant coliforms, lactic acid bacteria viability, instrumental color, and sensory analysis of fermented milks added to passion fruit peel flour (PFPF), throughout 29 days of storage, except composition. Four fermented milk treatments were prepared as follows: 1, fermented milk without addition of PFPF; 2, fermented milk added with 1%...

Author(s): Juliana Aparecida Célia, Marco Antônio Pereira da Silva, Kênia Borges de Oliveira, Jéssica Leal Freitas e Souza, Diene Gonçalves Souza, Ligia Campos de Moura, Richard Marins da Silva, Caroline Cagnin , Bheatriz Silva Morais de Freitas, Geovana Rocha Plácido and Márcio Caliari

Microbiological load of yoghurt sold in Omoku schools, Rivers State, Nigeria

August 2015

Sachets of various brands of yoghurt were randomly purchased from different retail outlets within Omoku schools and its pH and microbiological quality were determined using standard method. Total bacterial count (TBC) and Coliform count were done using standard plate count method after making serial dilutions of yoghurt samples. Nutrient agar (NA) was used for enumeration of TBC. NA plates were incubated at 37°C for...

Author(s): Chimezie Gabriel Dirisu, Gloria Lily and Ebere Igwe

Effects of lithium compounds on the growth of white-rot fungi

August 2015

Identifying the most suitable lithium compounds for fungal growth is important for the lithium (Li) enrichment of mushrooms. However, enrichment thus far has been carried out using LiCl. In an effort to identify an alternative source of lithium, we investigated the effect of five lithium compounds on lag phase, growth rate and biomass of ten species of white-rot fungi. The objective of this work was to make a screening...

Author(s): Mateus D. Nunes, Willian L. Cardoso, José M. R. Luz and Maria C. M. Kasuya

Detection of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae using the ESBL NDP test and flow cytometric assay in comparison to the standard disc diffusion

August 2015

This study was undertaken to evaluate the comparison among three different assays: extended-spectrum beta-lactamases (ESBL) Nordmann/ Dortet/ Poirel (NDP) test, flow cytometric assay and disc diffusion method for the detection of ESBL production. Sixty clinical isolates of Klebsiella pneumoniae were isolated from patients’ clinical samples admitted to Suez-Canal University Hospital, Ismailia Governorate. The...

Author(s): Samaa Taha, Nahed Youssef, Amany Elkazaz and Hazem Ramadan

Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria

August 2015

The aim of this work was to determine the antibiotic susceptibility pattern of the Staphylococcus aureus isolates from orthopaedic patients and their plasmid carriage. A total number of 39 S. aureus were isolated from wound, skin and bed of orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria with the use of API STAPH identification kit. Antibiotics susceptibility test was done using disc...

Author(s): Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B.

Carbon dioxide mitigation by microalga in a vertical tubular reactor with recycling of the culture medium

August 2015

Microalgae use photosynthesis as their principal metabolic mechanism to obtain organic carbon from the inorganic carbon contained in CO2 using solar energy. This process releases oxygen into the atmosphere. The aim of this study was to determine the most appropriate biomass concentration for growing Spirulina sp. LEB 18 (Cyanobacteria) in a vertical tubular photobioreactor with biomass removal and recycling of the...

Author(s): Michele Greque de Morais, Cleber Klasener da Silva, Adriano Arruda Henrard and Jorge Alberto Vieira Costa

Congruence between the drug resistance pattern of Escherichia coli and Proteus spp. isolated from humans and those from wild animals

August 2015

As human populations grow and transform landscapes, contact with wildlife concomitantly increases. Disease emergence has been an important consequence of these contacts, with many of emerging infectious diseases in humans arising from wildlife reservoirs. Drug resistance is a very important dimension of disease emergence and tracing the source is a veritable containment strategy. Escherichia coli and Proteus spp. have...

Author(s): Eze, E. A., Mustapha, J. K., Eze, C.N. and Enebe, M. C.

Physiological and sanitary potential of peanut seed treated with cinnamon powder

August 2015

This study aimed to assess the physiological and sanitary potential of peanut seeds cv. BR1, treated with powdered cinnamon (Cinnamomum zeylanicum). The experiment was conducted in the laboratories of the Seed and Plant Pathology and Microbiology at the Federal Rural University of Pernambuco / Academic Unit of Garanhuns (UFRPE / UAG). The experimental design was completely randomized, with four replications of 50 seeds....

Author(s): Adrielle Naiana Ribeiro Soares, Edilma Pereira Gonçalves, Jeandson Silva Viana, Priscila Cordeiro Souto and Mácio Farias de Moura

Isolation and identification of Sparicotyle chrysophrii (Monogenea: Microcotylidae) from gilthead sea bream (Sparus aurata L.) in the Mediterranean Sea, Greece

August 2015

The gilthead sea bream is one of the main aquacultured fish species in the Mediterranean Sea. Sparicotyle chrysophrii is an ectoparasite specific for sea bream and able to cause mortality in the Mediterranean aquaculture system. In this study, a total of 20 gilthead sea bream were sampled for parasitological research at Heraklion Bay, Crete, Greece. Gills of the fish were dissected and examined for parasites using a...

Author(s): Jin Woo Jun

Isolation and genetic characterization of endophytic and rhizospheric microorganisms from Butia purpurascens Glassman

August 2015

Interactions between plants and microorganism are complex and can affect the establishment of plant communities and change ecosystem properties. These interactions are of interest for researchers and have many ecological and biotechnological applications. The present study is the first report on the microbial diversity associated with Butia purpurascens Glassman, and the goal was to isolate and identify the genetic...

Author(s): Cintia Faria da Silva, Jaqueline Alves Senabio, Liliane Círa Pinheiro, Marcos Antônio Soares and Edson Luiz Souchie

Response of single and co-inoculation of plant growth promoting rhizobacteria on growth, flowering and nutrient content of chrysanthemum

August 2015

A pot experiment was conducted in the screen-house of Department of Horticulture, College of Agriculture, CCS Haryana Agricultural University, Hisar during the two successive seasons of 2011-12 and 2012-13 to investigate the potential effect of different strains of Bacillus (BS1- SYB101, BS2- SB155 and BS3- SB127), Pseudomonas (PS1- WPS73, PS2- CPA152 and PS3-P20) and their combination on growth, flowering and nutrient...

Author(s): Anop Kumari, R. K. Goyal, Mahesh Choudhary and S. S. Sindhu

Isolation and characterization of Lactobacillus plantarum BLS29 as a potential probiotic starter culture for pork sausage production

August 2015

The aim of this study was to isolate and characterize lactic acid bacteria (LAB) from naturally fermented pork sausages and test them for use as a probiotic starter culture in the production of fermented sausages. LAB (n=26) were isolated from natural fermented pork sausages. Isolates were identified using 16S rDNA or Internal Transcribed Spacer (ITS) region sequencing. After that, strains were characterized based on...

Author(s): Luciana Senter,, Jamile Queiroz Pereira, Manoela Alano Vieira, Eliane Maria Zandonai Michielin and Eduardo Cesar Tondo,

Isolation and optimization of amylase producing bacteria and actinomycetes from soil samples of Maraki and Tewedros campus, University of Gondar, North West Ethiopia

August 2015

The objective of the present study was to isolate, identify and optimize potential amylase producing bacteria and actinomycetes from soil samples. The soil samples were collected from Maraki and Tewedros campus, University of Gondar. Isolation was done by serial dilution and spread plate method. Primary screening of amylolytic activity of the isolates was performed by starch agar plate method. The submerged state...

Author(s): Gebreselema Gebreyohannes

Action of proteases of the nematophagous fungi Pochonia chlamydosporia on Ascaris suum eggs of collared peccary (Pecari tajacu)

August 2015

Among the parasites of domestic and wild swine, Ascaris suum stands out; a nematode that can lead to growth retardation and reduction in weight gain due to its action, especially in young animals. The objective of this study was to test the ovicidal action of proteases from Pochonia chlamydosporia (VC4) on A. suum eggs in an assay with Petri dishes. The fungus P. chlamydosporia (VC4) was grown in Erlenmeyers flasks with...

Author(s): Filippe Elias de Freitas Soares, José Humberto de Queiroz, Jackson Victor de Araújo, Maria Gorete Ramos Rodrigues, Alexandre de Oliveira Tavela, Anderson Rocha Aguiar, Tracy Lacerda,  Carolina Magri Ferraz, Maria Cristina Valdetaro Rangel,  Thiago Senna, Andreia Luíza Araújo, Tarcízio de Paula Rego, Caio Colodette Sena and Fabio Ribeiro Braga

Culturable bacterial diversity and hydrolytic enzymes from drass, a cold desert in India

August 2015

Bacterial diversity of composite soil sample of drass was explored and screened for various hydrolytic enzymes. About 600 bacterial strains were isolated using six different growth media, that is, R2A, nutrient agar, King’s B media, tryptic soy agar, Luria-Bertani agar and minimal media (100 isolates picked randomly from each media). These bacterial isolates were further differentiated on the basis of...

Author(s): Puja Gupta and Jyoti Vakhlu

Lactic acid bacteria associated with the digestive tract and skin of Sea bream (Sparus aurata) cultured in Tunisia

August 2015

Thirty-seven (37) enterococcal isolates were recovered from the skin and intestines of the sea bream (Sparus aurata), the most economically important fish species of the Mediterranean sea from Tunisian fish farming sites, to investigate their antimicrobial potential. All isolates were identified to the species level using genotypic tools. An investigation employing 16S rDNA sequencing in combination with randomly...

Author(s): Ouissal Chahad Bourouni, Jorge Barros-Velàzquez, Pilar Calo-Mata and Monia El Bour

Evaluation of hygienic-sanitary quality of honey from Apis mellifera L. obtained in semi-arid region of Piauí, Brazil

July 2015

This study aimed to evaluate the quality of honey from Apis mellifera L. obtained in Piauí, Brazil. The completely randomized design (CRD) was used in the experiments. Two treatments of honey were prepared: one from beekeepers that use Extraction Units for Bee Products (EUBP) with Best practices for beekeeping (T1), and another one from those which use EUBP without the best practices (T2). Parameters analyzed...

Author(s): Rosana Martins Carneiro Pires, Sinevaldo Gonçalves de Moura, Francisco das Chagas Cardoso Filho, Aline Marques Monte, Lúcio Fernandes Pires, Maria Cristina Affonso Lorezon, Rômulo Oliveira Barros, Maria Marlúcia Gomes Pereira and Maria Christina Sanches Muratori

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

July 2015

The major human fungal pathogens are species from the genus Candida, especially Candida albicans. C. albicans sample preservation from human source is essential for clinical purposes and epidemiologic studies; however, little is known about its conservation for DNA extraction and analysis. Therefore, the aim of this study was to evaluate and compare two storage conditions of C. albicans for performing DNA extraction and...

Author(s): Alessandro Menna Alves, Camila Perelló Ferrúa, Pedro Henrique de Azambuja Carvalho, Sandra Beatriz Chaves Tarquinio, Adriana Etges, Fernanda Nedel, and Rafael Guerra Lund

Evaluation of antibacterial activities of Barleria Prionitis Linn

July 2015

In the present study, 1 g/ml each of Barleria prionitis leaves (BPL) and B. prionitis stem (BPS) were extracted from different solvents like petroleum ether, chloroform ethyl acetate and methanol. Ethyl acetate extract of BPL showed maximum inhibition zone on Gram positive Bacillus pumilus (9.83 mm) and methanol extract of BPS showed minimum inhibition zone on Gram negative Escherichia coli (0.16 mm). Petroleum ether...

Author(s): Patel Bharatkumar K., Chandel B. S., Chauhan H. C., Patel Kirit B., Parth Falguni M., Patel Manoj V., Patel Sanjiv I., Pandya R. P. and Shah Jignesh D.

Bacteriophage based self-assembled monolayer (SAM) on gold surface used for detection of Escherichia coli by electrochemical analysis

July 2015

Bacteriophages (or phages) are parasites that infect specific bacteria. This host-selectivity can be useful to identify bacterial contaminants in food, water, environment etc. In the present study, phage was isolated from stagnant water and cultivated by overlay method against host bacteria that is E. coli. Phage titer was calculated to be 107 pfu/ml using 10-fold dilutions. Plaque reaction activity was observed...

Author(s): Vipin Singh,  Pranay Jain and Swati Dahiya

Effect of processing steps and storage on microbiota and volatile amines content of salted semi preserved cuttlefish (Sepia officinalis)

July 2015

Microbial and volatile amines changes during processing steps and storage in optimal and abuse temperatures of semipreserved cuttlefish and their efficiency for quality was investigated. After osmotic treatment the initial microbial contamination and the total volatile basic nitrogen (TVBN) decreased sharply. The microbiota changed to Gram positive bacteria in which species of Bacillus and Staphylococcus were dominant....

Author(s): Ibrahima Cissé, Manuela Hernandez-Herrero, Artur X. Roig-Sagues and Buenaventura Guamis Lopes

Cultivation and nutritional studies of an edible mushroom from North Brazil

July 2015

The vertical mycelial growth to investigate the feasibility of Pleurotus ostreatoroseus DPUA 1720 production in lignocellulosic Amazonic residues was evaluated. Mycelial development was carried out in cupuaçu exocarp (Theobroma grandiflorum Willd Former Spreng Schum), açai seed (Euterpe oleracea) and sawdust as substrates. Each residue was supplemented with rice bran, crown and pineapple peel. The average...

Author(s): Tamiris Rio Branco da Fonseca, Taciana de Amorim Silva, Mircella Marialva Alecrim, Raimundo Felipe da Cruz Filho and Maria Francisca Simas Teixeira

Occurrence of begomoviruses in cotton-vegetable agro ecosystem in India

July 2015

Begomoviruses are the important pathogens of variety of crop plants and are responsible for causing huge economic losses. It is assumed that cotton vegetable agro eco system plays a vital role in the complex etiology of the diseases caused by begomoviruses. Therefore, the present work was planned to study the relationship among begomoviruses infecting cotton, chilli, radish, tomato and papaya. For this purpose, the...

Author(s): S. I. Kaur, S. S. Kang and A. Sharma

Phosphate solubilizing rhizospheric bacterial communities of different crops of Korea District of Chhattisgarh, India

July 2015

Occurrence of Phosphate Solubilizing Bacteria (PSB) in the rhizospheric soil of different crops of Korea District of Chhattisgarh, India was studied. Phosphate solubilizing bacteria colonize the plant roots, affect plant growth positively and add nutrients to the soil. A good number of PSB were isolated, purified and identified from twenty-five different representative soil samples collected from five blocks of Korea...

Author(s): Swati Rose Toppo and Preeti Tiwari

Effects of practices of maize farmers and traders in Ghana on contamination of maize by aflatoxins: Case study of Ejura-Sekyeredumase Municipality

July 2015

Contamination of maize by aflatoxins is of major concern to governments and the international community because high degrees of aflatoxin in food render the food unsafe for human consumption. The disposal of such foods also constitutes an economic loss in food production. This paper reports the findings of a study conducted during the 2013 minor maize farming season in Ejura-Sekyeredumase Municipality in the Ashanti...

Author(s): Joseph Oppong Akowuah, Lena Dzifa Mensah, Chian Chan and Anthony Roskilly

In vitro antagonistic activity of Pseudomonas spp. against Rhizoctonia soloni

July 2015

The present research work deals with the in-vitro study of antagonistic activity of Pseudomonas spp. against plantpathogenic fungi Rhizoctonia soloni. The bacterial strains were isolated from rhizospheric soil of Korea district of Chhattisgarh. A total of 28 bacterial cultures were isolated from 25 representative soil samples collected from five blocks of Korea district of Chhattisgarh, out of which four were identified...

Author(s): Swati Rose Toppo and Preeti Tiwari

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